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Chinese Journal of Chemistry 2011年第8期29卷 1764-1768页

作者：张金振赵静周金慧薛晓峰李熠吴黎明陈芳 bee Products Quality Supervision and Testing center Institute of Apicultural Research Chinese Academy of Agricultural Sciences Beijing 100093 China

Amantadine （AMA） is an anti-viral drug used in apiculture to protect honeybee against the sacbrood virus （Morator aetatulae）. This study described a reliable high-performance liquid chromatographic （HPLC） method for analyzing AMA in honey using a solid-phase extraction （SPE） cartridge （Plexa PCX） for purification, 4-fluoro-7- nitro-2,1,3-benzoxadiazole （NBD-F） as a pre-column derivatization agent, and fluorometric detection （λex =470 nm, λem=530 nm）. The chromatographic separation was performed on an XDB C18 column （150×4.6 mm i.d.） using 0.1% trifluoroacetic acid/acetonitrile （35 ; 65, V/V） as the mobile phase at a flow rate of 1.0 mLomin 1 with a run time of 20 min. Under these optimal conditions, a linear relationship was observed in the range of 0.025--1.0μg·mL-1 with a good correlation coefficient （0.998） and low limit of detection （0.0080 μg·g-1）, the recoveries were all above 90%, and the intra-day and inter-day precision （RSD） ranged from 3.4%--5.1%.

关键词： amantadine honey solid-phase extraction high-performance liquid chromatographic (HPLC),fluorometric detection

Effects of Imidacloprid and Varroa destructor on survival and health of European honey bees, Apis mellifera

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Insect Science 2017年第3期24卷 467-477页

作者： Pendo M. Abbo Joshua K. Kawasaki Michele Hamilton Steven C. Cook Gloria DeGrandi-Hoffman Wen Feng Li Jie Liu Yan Ping Chen Department of Biomedical Engineering Columbia University New York New York Department of Microbiology & Molecular Biology Brigham Young University Provo Utah USDA-ARS bee research Laboratory Beltsville Maryland USDA-ARS Carl Hayden bee research center Tucson Arizona USA College of bee Science Fujian Agriculture and Forestry University Fuzhou China

There has been growing concern over declines in populations of honey bees and other pollinators which are a vital part to our food security. It is imperative to identify factors responsible for accelerated declines in bee populations and develop solutions for reversing bee losses. While exact causes of colony losses remain elusive, risk factors thought to play key roles are ectoparasitic mites Varroa destructor and neonicotinoid pesticides. The present study aims to investigate effects of a neonicotinoid pesticide Imidacloprid and Varroa mites individually on survivorship, growth, physiology, virus dynamics and immunity of honey bee workers. Our study provides clear evidence that the exposure to sublethal doses of Imidacloprid could exert a significantly negative effect on health and survival of honey bees. We observed a significant reduction in the titer ofvitellogenin （Vg）, an egg yolk precursor that regulates the honey bees development and behavior and often are linked to energy homeostasis, in bees exposed to lmidacloprid. This result indicates that sublethal exposure to neonicotinoid could lead to increased energy usage in honey bees as detoxification is a energy-consuming metabolic process and suggests that Vg could be a useful biomarker for measuring levels of energy stress and sublethal effects of pesticides on honey bees. Measurement of the quantitative effects of different levels of Varroa mite infestation on the replication dynamic of Deformed wing virus （DWV）, an RNA virus associated with Varroa infestation, and expression level of immune genes yields unique insights into how honey bees respond to stressors under laboratory conditions.

关键词： Deformed wing virus honey bees Imidacloprid innate immunity Varroa

Identification of Cyprus propolis composition and evaluation of its antimicrobial and antiproliferative activities

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Food Bioscience 2023年第1期51卷 439-446页

作者： Sual Tatlısulu Erkay Ozgor Department of Bioengineering Faculty of EngineeringCyprus International UniversityCyprus Department of Molecular Biology and Genetics Faculty of Art and ScienceCyprus International UniversityCyprus Cyprus bee and bee Products research center Cyprus International UniversityCyprus

Propolis is a raw natural product produced by Apis mellifera for hive *** have also investigated propolis in terms of different biological activities such as antimicrobial and anticancer effects for many *** activities depend on the chemical composition of propolis and the composition is changed according to the botanical origin and sampling *** this study,13 propolis samples were collected from different locations in Cyprus and ethanolic extraction of Cyprus Propolis(EECP)composition was analysed by GC-MS *** total,there are 78 compounds were found in *** concentrations(200,100 and 50μg/ml)of EECP treated with breast cancer cell lines(MCF-7 and MDA-MB-231)at 24 h and 48 h for determined antiproliferation *** EECP,except EECP5 and EECP6,inhibit proliferation activity at all *** between exception samples and all others is the deficiency of 8-β*** concentrations of EECP also treated with nosocomial bacteria and results showed EECPs prevented proliferation on Escherichia coli and Pseudomonas aeruginosa while no effect observed on Klebsiella *** situation might be due to the fact that *** is *** conclusion,it has been determined that Cyprus propolis,which contains different active compounds,especially 8-βH-cedran-8-ol,is a potential product against infectious diseases and breast cancer proliferation.

关键词： Propolis GC-MS MCF-7 MDA-MB-231 E.coli P.Aeruginosa

Next generation sequencing of Apis mellifera syriaca identifies genes for Varroa resistance and beneficial bee keeping traits

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Insect Science 2016年第4期23卷 579-590页

作者： Nizar Haddad Ahmed Mahmud Batainh Osama Suleiman Migdadi Deepti Saini Venkatesh Krishnamurthy Sriram Parameswaran Zaid Alhamuri bee research Department National Center for Agricultural Research and Extension P.O.Box 639-Baqa 19381 jordanian bee research Station National Center for Agricultural Research and Extension Irbid Jordan research and Development Unit Genotypic Technology (P) Ltd. #259 Apurva 2nd Floor 4th Cross R.M. V. 2nd Stage 80 Feet Road Bangalore 560 094 India

Apis mellifera syriaca exhibits a high degree of tolerance to pests and pathogens including varroa mites. This native honey bee subspecies of Jordan expresses behavioral adaptations to high temperature and dry seasons typical of the region. However, persistent honey bee imports of commercial breeder lines are endangering local honey bee population. This study reports the use of next-generation sequencing （NGS） technology to study the A. m. syriaca genome and to identify genetic factors possibly contributing toward mite resistance and other favorable traits. We obtained a total of 46.2 million raw reads by applying the NGS to sequence A. m. syriaca and used extensive bioinformatics approach to identify several candidate genes for Varroa mite resistance, behavioral and immune responses char- acteristic for these bees. As a part of characterizing the functional regulation of molecular genetic pathway, we have mapped the pathway genes potentially involved using information from Drosophila melanogaster and present possible functional changes implicated in responses to Varroa destructor mite infestation toward this. We performed in-depth functional annotation methods to identify -600 candidates that are relevant, genes involved in pathways such as microbial recognition and phagocytosis, peptidoglycan recognition protein family, Gram negative binding protein family, phagocytosis receptors, serpins, Toll signaling pathway, Imd pathway, Tnf, JAK-STAT and MAPK pathway, heamatopioesis and cellular response pathways, antiviral, RNAi pathway, stress factors, etc. were selected. Finally, we have cataloged function-specific polymorphisms between A. mellifera and A. m. syriaca that could give better understanding of varroa mite resistance mechanisms and assist in breeding. We have identified immune related embryonic development （Cactus, Relish, dorsal, Ank2, baz）, Varroa hygiene （NorpA2, Zasp, LanA, gasp, impl3） and Varroa resistance （Pug, pcmt, elk, elf3-s10, Dscam2, Dhc64C, gro,

关键词： Apis mellifera syriaca breeding conservation next-generation sequencing(NGS) varroa mite resistance

Comparative susceptibility and immune responses of Asian and European honey bees to the American foulbrood pathogen, Paenibacillus larvae

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Insect Science 2019年第5期26卷 831-842页

作者： Sasiprapa Krongdang Jay D.Evans Yanping Chen Wannapha Mookhploy Panuwan Chantawannakul 1bee Protection Laboratory Department of BiologyFaculty of ScienceChiang Mai UniversityChiang MaiThailand Interdisciplinary Program in Biotechnology Graduate SchoolChiang Mai UniversityChiang MaiThailand USDA-ARS Bee Research LaboratoryBeltsvilleMarylandUSA Environmental Science research center Faculty of ScienceChiang Mai UniversityChiang MaiThailand

American foulbrood (AFB) disease is caused by Paenibacillus larvae. Currently, this pathogen is widespread in the European honey bee— Apis mellifera. However, little is known about infectivity and pathogenicity of P. lan'ae in the Asiatic cavity-nesting honey bees, Apis cerana. Moreover, comparative knowledge of P. larvae infectivity and pathogenicity between both honey bee species is scarce. In this study, we examined susceptibility, larval mortality, survival rate and expression of genes encoding antimicrobial peptides (AMPs) including defensin, apidaecin, abaecin, and hymenoptaecin in A. mellifera and A. cerana when infected with P. larvae. Our results showed similar effects of P. larvae on the survival rate and patterns of AMP gene expression in both honey bee species when bee larvae are infected with spores at the median lethal concentration (LC5 0 ) for A. mellifera. All AMPs of infected bee larvae showed significant upregulation compared with noninfected bee larvae in both honey bee species. However, larvae of A. cerana were more susceptible than A. mellifera when the same larval ages and spore concentration of P. larvae were used. It also appears that A. cerana showed higher levels of AMP expression than A. mellifera. This research provides the first evidence of survival rate, LC50 and immune response profiles of Asian honey bees, A. cerana, when infected by P. larvae in comparison with the European honey bee, A. mellifera.

关键词： Apis cerana Apis mellifera honey bee immune gene Paenibacillus larvae susceptibility

research progress on distribution,sources,identification,toxicity,and biodegradation of microplastics in the ocean,freshwater,and soil environment

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Frontiers of Environmental Science & Engineering 2022年第1期16卷 5-18页

作者： Qinghui Sun Juan Li Chen Wang Anqi Chen Yanli You Shupeng Yang Huihui Liu Guibin Jiang Yongning Wu Yanshen Li College of Life Science Yantai UniversityYantai 264005China Department of Chemical and Biochemical Engineering University of Western OntarioLondon ON N6A5B9Canada Institute of Apicultural research Chinese Academy of Agricultural SciencesKey Laboratory of Bee Products for Quality and Safety ControlLaboratory of Risk Assessment for Quality and Safety of Bee ProductsBee Product Quality Supervision and Testing CenterMinistry of AgricultureBeijing 100093China Shandong Marine Resource and Environment research Institute Laboratory of Restoration for Marine EcologyYantai 264006China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China NHC Key Laboratory of Food Safety Risk Assessment Chinese Academy of Medical Science Research Unit(2019RU014)China National Center for Food Safety Risk AssessmentBeijing 100017China

Microplastics(MPs)are distributed in the oceans,freshwater,and soil environment and have become major *** are generally referred to as plastic particles less than 5 mm in *** consist of primary microplastics synthesized in microscopic size manufactured production and secondary microplastics generated by physical and environmental *** particles are long-lived pollutants that are highly resistant to environmental *** this review,the distribution and possible sources of MPs in aquatic and terrestrial environments are ***,the adverse effects of MPs on natural creatures due to ingestion have been *** also have summarized identification methods based on MPs particle size and chemical *** control the pollution of MPs,the biodegradation of MPs under the action of different microbes has also been reviewed in this *** review will contribute to a better understanding of MPs pollution in the environment,as well as their identification,toxicity,and biodegradation in the ocean,freshwater,and soil,and the assessment and control of microplastics exposure.

关键词： Microplastics Distribution Toxicity Identification Biodegradation Environment

The Status of Honey Quality Produced in Gedebano Gutazer Wolene,Central Ethhiopia

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Journal of Food Science and Engineering 2020年第3期10卷 110-119页

作者： Akalework Gizaw Asaminew Tassew Desalegn Begna Ministry of Agriculture Addis AbabaP.O.Box 62347Ethiopia Colleges of Agriculture and Environmental Sciences Bahir Dar UniversityP.O.Box 5501Ethiopia holleta bee research center P.O.Box 22Ethiopia

The study was conducted aiming at evaluating the status of the physicochemical properties of honey produced in Gedebano Gutazer Wolene District of SNNP,Central Ethiopia in 2016/*** evaluate the physicochemical properties of honey,20 samples of honey having 1 kg each was collected purposely from traditional&frame hive at farm-gate level of three different agro-ecological locations&two rural *** results were compared with National,European and International honey quality standard *** the results were compared between different locations,market and farm-gate level as well as between traditional and frame hive honey *** physicochemical parameters of honey quality conducted in the analysis were color,moisture content,HMF(hydroxyl methyl furfural),free acidity,pH,ash,electro-conductivity,sugar content,sucrose&*** to the analysis,except HMF which shows significant(p0.05)differences between *** the results of quality parameters indicated to be within the range of National,European and International quality standard *** mean values of the collected honey samples were:moisture content(18.91%),electro-conductivity(0.65 mS/m),free acid(16.3 meq/kg),HMF(2.63 mg/kg),ash content(0.2 g/100g),sugar content(74.4%),sucrose(1.06%),maltose(0.95%)and the range of honey color was 3.3-3.96 mm p-fund *** results obtained therefore indicate that honey produced in the district shows excellent quality and is free of any adulterants in relation to National,European and International limits so that it can be exported.

关键词： Honey quality physicochemical hive type agro ecology standard

Survival of boll weevil （Coleoptera： Curculionidae） adults after feeding on pollens from various sources

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Insect Science 2007年第6期14卷 503-510页

作者： SHOIL M. GREENBERG GRETCHEN D. JONES FRANK EISCHEN RANDY J. COLEMAN JOHN J. ADAMCZYK, JR. TONG-XIAN LIU MAMOUDOU SETAMOU USDA-ARS KSARC-BIRU Weslaco USDA-ARS APMRU College Station USDA-ARS KSARC-Honey Bee Research Unit Department of Entomology Texas AgriLife Research Texas A&M University System Texas A&M University Kingsville Citrus center Weslaco Texas USA

The survival of overwintering boll weevil, Anthonomus grandis grandis （Boheman）, adults on non-cotton hosts in the Lower Rio Grande Valley （LRGV） of Texas was examined from 2001 to 2006. The success of the Boll Weevil Eradication Program, which was reintroduced into the LRGV in 2005, depends on controlling overwintering boll weevil populations. Laboratory studies were conducted using boll weevil adults that were captured in pheromone traps from September through March. The number of adults captured per trap declined significantly in the field from fall to the beginning of spring （3.5-7.0-fold）. The proportion of trapped males and females did not differ significantly. The mean weight of boll weevil adults captured in September was 13.3 mg, while those of captured adults from November to February were significantly lower and ranged from 6.7 to 7.8 mg. Our results show that boll weevil adults can feed on different plant pollens. The highest longevity occurred when adults were fed almond pollen or mixed pollens （72.6 days and 69.2 days, respectively） and the lowest when they fed on citrus pollen or a non-food source （9.7 days or 7.4 days, respectively）. The highest adult survival occurred on almond and mixed pollens [88.0%-97. 6% after 1st feeding period （10 days）, 78.0%-90.8% after 3rd feeding period （10 days）, 55. 0%-83.6% after 5th feeding period （10 days）, and 15.2%-32.4% after lOth feeding period （10 days）]. The lowest adult survival occurred on citrus pollen [52.0%-56.0% after 1st feeding period （10 days）, 13.3% after 3rd and 5th feeding periods （10 days）, and 0 after 6th feeding period （10 days）]. Pollen feeding is not a behavior restricted to adult boll weevils of a specific sex or physiological state. Understanding how boll weevil adults survive in the absence of cotton is important to ensure ultimate success of eradicating this pest in the subtropics.

关键词： boll weevil Anthonomus grandis grandis Curculionidae subtropics pollen

Distribution and variability of deformed wing virus of honeybees （Apis mellifera） in the Middle East and North Africa

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Insect Science 2017年第1期24卷 103-113页

作者： Nizar Jamal Haddad Adjlane Noureddine Banan Al-Shagour Wahida Loucif-Ayad Mogbel A. A. El-Niweiri Eman Anaswah Wafaa Abu Hammour Dany El-Obeid Albaba Imad Mohamed A. Shebl Abdulhusien Sehen Almaleky Abdullah Nasher Nagara Walid Mohamed Fouad Bergigui Orlando Yanez Joachim R. de Miranda bee research Department National Center for Agriculture Research and Extension Baq'a Jordan Department of Biology M'hamed Bougare University of Boumerdes ENS Kouba Algeries Laboratory of Applied Animal Biology University Badji-Mokhtar Annaba Algeria Department of bee research Environment Natural Resources and Desertification Research Institute National Centre for Research Khartoum Sudan Faculty of Agriculture and Veterinary Sciences Lebanese University Beirut Lebanon West Bank State of Palestine HalhuI-Hebron District Palestine Department of Plant Protection Suez Canal University Ismailia Egypt Extension Department Qadysia Govemate Agricultural Directorate Iraq Department of Plant Protection Sana'a University Sane'a Yemen National Federation of Tunisian beekeepers Tunis Tunisia Ruchers El Bakri Hay Assalam-Sidi Slimane Rabat Morocco Institute of bee Health Vetsuisse Faculty University of Bern Bern Switzerland Department of Ecology Swedish University of Agricultural Sciences Uppsala Sweden

Three hundred and eleven honeybee samples from 12 countries in the Middle East and North Africa （MENA）（Jordan, Lebanon, Syria, Iraq, Egypt, Libya, Tunisia, Algeria, Morocco, Yemen, Palestine, and Sudan） were analyzed for the presence of deformed wing virus （DWV）. The prevalence of DWV throughout the MENA region was pervasive, but variable. The highest prevalence was found in Lebanon and Syria, with prevalence dropping in Palestine, Jordan, and Egypt before increasing slightly moving westwards to Algeria and Morocco Phylogenetic analysis of a 194 nucleotide section of the DWV Lp gene did not identify any significant phylogenetic resolution among the samples, although the sequences did show consistent regional clustering, including an interesting geographic gradient from Morocco through North Africa to Jordan and Syria. The sequences revealed several clear variability hotspots in the deduced amino acid sequence, which furthermore showed some patterns of regional identity. Furthermore, the sequence variants from the Middle East and North Africa appear more numerous and diverse than those from Europe.

关键词： Apis mellifera deformed wing virus Middle East North Africa prevalence variability

Biased dispersal of Metrioptera bicolor, a wing dimorphic bush-cricket

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Insect Science 2018年第2期25卷 297-308页

作者： Ina Monika Margret Heidinge Silke Hein Heike Feldhaar Hans-Joachim Poethke Field Station Fabrikschleichach University of W（Jrzburg Rauhenebrach Germany W~sseri 8 CH-8816 Hirzel Switzerland Department of Animal Ecology I University of Bayreuth Bayreuth Germany Bavarian State Institute for Viticulture and Horti- culture Bee Research Center An der Steige 15 97206 VeitshSchheim Germany

In the highly fragmented landscape of central Europe, dispersal is of particular importance as it determines the long-term survival of animal populations. Dispersal not only secures the recolonization of patches where populations went extinct, it may also rescue small populations and thus prevent local extinction events. As dispersal involves different individual fitness costs, the decision to disperse should not be random but context- dependent and often will be biased toward a certain group of individuals （e.g., sex- and wing morph-biased dispersal）. Although biased dispersal has far-reaching consequences for animal populations, immediate studies of sex- and wing morph-biased dispersal in orthopterans are very rare. Here, we used a combined approach of morphological and genetic analyses to investigate biased dispersal of Metrioptera bicolor, a wing dimorphic bush-cricket. Our results clearly show wing morph-biased dispersal for both sexes of M. bicolor. In addition, we found sex-biased dispersal for macropterous individuals, but not for micropters. Both, morphological and genetic data, favor macropterous males as dispersal unit of this bush-cricket species. To get an idea of the flight ability ofM. bicolor, we compared our morphological data with that of Locusta migratoria and Schistocerca gregaria, which are very good flyers. Based on our morphological data, we suggest a good flight ability for macropters of M. bicolor, although flying individuals of this species are seldom observed.

关键词： aspect ratio dispersal traits flight muscle ratio Metrioptera bicolor sex-biased dispersal wing load wing morph-biased dispersal