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Regeneration of rat corpora cavernosa tissue by transplantation of CD133＋ cells derived from human bone marrow and placement of biodegradable gel sponge sheet

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Asian Journal of Andrology 2017年第2期19卷 203-207页

作者： Shogo Inoue Katsutoshi Miyamoto Shunsuke Shinmei Koichi Shoji Jun Teishima Kazuhiro Sentani Wataru Yasui Akio Matsubara Department of Urology Hiroshima University Institute of Biomedical and Health Sciences Hiroshima Japan Department of Molecular Pathology Hiroshima University Institute of Biomedical and Health Sciences Hiroshima Japan

The objective is to develop an easier technique for regenerating corpora cavernosa tissue through transplantation of human bone marrow-derived CD133＋ cells into a rat corpora cavernosa defect model. We excised 2 mm× 2 mm squares of the right corpora cavernosa of twenty-three 8-week-old male nude rats. AIginate gel sponge sheets supplemented with 1 × 10^4 CD133＋ cells were then placed over the excised area of nine rats. Functional and histological evaluations were carried out 8 weeks later. The mean intracavernous pressure/mean arterial pressure ratio for the nine rats （0.34258 ± 0.0831） was significantly higher than that for eight rats with only the excision （0.0580±0.0831, P = 0.0238） and similar to that for five rats for which the penis was exposed, and there was no excision （0.37228±0.1051, P = 0.8266）. Immunohistochemical analysis revealed that the nine fully treated rats had venous sinus-like structures and quantitative reverse transcription polymerase chain reaction analysis of extracts from their alginate gel sponge sheets revealed that the amounts of mRNA encoding the nerve growth factor （NGF）, and vascular endothelial growth factor （VEGF） were significantly higher than those for rats treated with alginate gel sheets without cell supplementation （NGF： P= 0.0309; VEGF： P〈 0.0001）. These findings show that transplantation of CD133＋ cells accelerates functional and histological recovery in the corpora cavernosa defect model.

关键词： alginate gel sponge sheet CD133+ cell corpora cavernosa

Ammonium Removal from Ammonium Rich Solution by Bio Char

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Journal of Environmental Protection 2022年第1期13卷 171-182页

作者： Harada Hiroyuki Akagi Kotaro Endar Hidayat Department of Environmental Science Prefectural University of Hiroshima Shobara Hiroshima Japan

When biochar made from waste pallet was added to treated livestock wastewater, the total nitrogen and ammonium ion concentrations decreased, with removal rates over 10 days of about 60% and 30%, respectively. Bacteria were isolated with high ammonium removal ability and they were identified based on their 16S rRNA gene sequences. Anaerobic denitrifying Cronobacter spp. was isolated from the biochar used for water purification. When each strain was cultured in a liquid medium containing ammonium sulfate (initial ammonium concentration 30 mg/L), the highest ammonium removal rates were 83.8% - 96.5%. Organic acids were more effective than carbohydrates as sole carbon sources for nitrogen removal from wastewater. The absorbance at 660 nm increased with nitrogen removal, indicating that cells proliferated, so it was presumed that ammonium was taken up by assimilation.

关键词： Livestock Wastewater Ammonium Removal Rate Cronobacter spp. 16S rRNA

River system in Japan from a landscape ecological aspect

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Journal of Environmental Sciences 2003年第2期15卷 160-166页

作者： Nobukazu NAKAGOSHI Masahito INOUE Graduate School for International Development and Cooperation Hiroshima UniversityGraduate School for International Development and Cooperation Hiroshima University 1-5-1 Kagamiyama Higashi-Hiroshima 739-8529 Japan1-5-1 Kagamiyama Higashi-Hiroshima 739-8529 Japan

The objective of this study was to elucidate characteristics associated with rivers by classification of major rivers in Japan into several types based on riparian conditions. As the results of principal component analysis (PCA) with use of parameters reflecting forms and artificial alterations of respective rivers, four major components such as “comprehensive riparian size', “intactness of water front', “continuity of streams' and “simplicity of landform in river basin' were extracted. Subsequently, cluster analysis was performed based on principal component scores, leading to successful classification of major rivers into 6 types. These findings disclosed that (1) the extracted principal components provide effective viewpoint for classification of rivers; (2) distribution of respective classes indicates area properties; and (3) the employed quantitative procedures were found effective for classification of major rivers.

关键词： river system landscape ecological aspect Japan

Current treatment strategies and future perspectives for gastrointestinal stromal tumors

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World Journal of Gastrointestinal Pathophysiology 2022年第1期13卷 15-33页

作者： Yoichi Sugiyama Masaru Sasaki Mohei Kouyama Tatsuya Tazaki Shinya Takahashi Atsushi Nakamitsu Department of Gastrointestinal Surgery JA Hiroshima General HospitalHatsukaichi 738-8503HiroshimaJapan Department of Surgery Institute of Biomedical and Health SciencesHiroshima UniversityHiroshima 734-8551Japan

Gastrointestinal stromal tumors(GISTs)are mesenchymal tumors that originate from the gastrointestinal tract,mostly from the *** are derived from the myenteric interstitial cells of Cajal and are caused by several mutations in the c-kit and platelet-derived growth factor receptor ***,GISTs are detected by endoscopic and imaging findings and are diagnosed by *** is the first line of treatment,and if the tumor is relatively small,minimally invasive surgery such as laparoscopy is *** recent years,neoadjuvant therapy has been administered to patients with GISTs that are suspected of having a large size or infiltration to other *** adjuvant imatinib is the standard therapy for high-risk *** is important to assess the risk of recurrence after GIST ***,the effect of tyrosine kinase inhibitor use will vary by the mutation of c-kit genes and the site of ***,information regarding gene mutation is indispensable when considering the treatment policy for recurrent *** article reviews the clinicopathological characteristics of GISTs along with the minimally invasive and multidisciplinary treatment options available for these *** future perspectives for diagnostic and treatment approaches for these tumors have also been discussed.

关键词： Gastrointestinal stromal tumor Minimally invasive surgery Laparoscopic surgery Imatinib Neoadjuvant therapy Risk assessment

A trial of simple and easy health monitoring for power poles by using ‘ipod’

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Science China(Technological Sciences) 2015年第4期58卷 712-719页

作者： ORAI Naoki ICHII Koji ISHII Shiho Graduate School of Engineering Hiroshima University

At present,the regular inspection of power poles is just a visual inspection based on the inspector’s ***,overlook of the deteriorated poles can come *** order to avoid an accident due to the overlook of the deteriorated poles,the authors propose a simple and easy health monitoring method for power poles using‘i Pod’.Since the applicability of‘i Pod’as a vibration sensor in this purpose was not clear,it was examined in the following ***,the authors proposed a tool to fix the‘i Pod’to a power pole,and the performance of‘i Pod’as a vibration measurement device was compared with a conventional acceleration *** a result,it was confirmed that‘i Pod’could give almost the same response with the conventional sensor in terms of the observed predominant ***,vibration measurements were conducted at power poles in a *** a result,the observed predominant frequency corresponded to the calculated 2nd natural frequency of power *** some power poles were supported by some additional wires from the ground,the measurements were successful at almost all the ***,the authors confirmed that the‘i Pod’based health monitoring is possible.

关键词： iPod 健康监测振动传感器电杆试用加速度传感器固有频率目视检查

Cellular Growth Dynamics Affects Allelopathic Activity in Coffee Cell Culture

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American Journal of Plant Sciences 2023年第8期14卷 857-868页

作者： Muchamad Imam Asrori Shinjiro Ogita Graduate School of Comprehensive Scientific Research Prefectural University of Hiroshima Hiroshima Japan

Cellular growth dynamics and allelopathic activity in coffee cell cultures were examined as follows: First, we compared allelopathic activity of seven woody plant calli, Coffea canephora, Derris indica, Ficus carica L., Juniperus conferta, Prunus persica, Punica granatum, and Sonneratia ovata, using a modified “sandwich method bioassay” and found that coffee callus showed the strongest growth inhibition to lettuce seedling nearly 90% of hypocotyl and 96% of root. This coffee callus actively proliferated, with a 21-fold increase during five weeks of subculture, with a growth curve comprising two typical phases: a lag phase of 0 - 2 weeks of culture and an exponential phase of 3 - 5 weeks of culture. Allelopathic activity varied depending on the growth phase of the coffee callus. The strongest allelopathic activity was detected in 1 - 2-week-old callus showing nearly 100% inhibitory effect on lettuce seedling growth. As the allelopathic activity of coffee calli is extremely high, beyond the natural level in coffee leaves and green beans, we focused on analyzing the allelopathic activity of its aqueous extracts using high-performance liquid chromatography. Several prominent peaks, including two reference alkaloids, theobromine and caffeine, which are known allelochemicals in coffee plants, and three distinct unknown peaks were identified at 270 nm in coffee calli during the lag phase (1 - 2 weeks of culture). The higher value of the total phenolic content in the lag phase also suggested a key biosynthetic pathway in relation to the allelopathic activity of coffee callus will be activated in the lag phase.

关键词： Allelopathic Activity Coffea canephora Callus Growth Phase Sandwich Method Bioassay

Discovery of Paleozoic rocks at northern margin of Sambagawa terrane,eastern Kyushu,Japan:Petrogenesis,U-Pb geochronology and its tectonic implication

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Geoscience Frontiers 2020年第4期11卷 1441-1459页

作者： Kenta Kawaguchi Yasutaka Hayasaka Tomoyuki Shibata Masayuki Komatsu Kosuke Kimura Kaushik Das Department of Earth and Planetary Systems Science Graduate School of ScienceHiroshima University1-3-1 KagamiyamaHigashi-HiroshimaHiroshima739-8526Japan hiroshima Institute of Plate Convergence Region Research 1-3-1 KagamiyamaHigashi-HiroshimaHiroshima739-8526Japan Ehime University 10-13 Dogo-HimataMatsuyamaEhime790-8577Japan

Ordovician diorite-quartz diorite mylonite(Saganoseki quartz diorite) was discovered in Sambagawa metamorphic terrane at the northern margin of Saganoseki Peninsula,Kyushu Island,*** LA-ICP-MS zircon U-Pb geochronology revealed that the intrusion age of Saganoseki quartz diorite was 473.3±3.6 *** rocks show the volcanic arc affinity based on the trace element *** the basis of geochronological and geochemical results,Saganoseki quartz diorite is considered to be a member of the Early Paleozoic igneous rocks of the Kurosegawa tectonic *** quartz diorite is located just south of the Median Tectonic Line(MTL)and is in close contact with the pelitic and psammitic schist without any brittle shear zone.U-Pb ages of detrital zircon grains from two psammitic schists show the estimated sedimentation age of early Late Cretaceous,indicate that these psammitic schists are the member of Sambagawa metamorphic *** with these results and the mode of occurrence in the field,we argue that the Early Paleozoic igneous rocks of the Kurosegawa tectonic zone existed as an upper structural unit of the Sambagawa *** relationship is the key to reconstruct the Mesozoic tectonics of Japan as a part of East Asia,and its evolution through time.

关键词： Outer zone of southwest Japan Sambagawa metamorphic terrane Kurosegawa tectonic zone LA-ICP-MS zircon U-Pb age Saganoseki quartz diorite

Rational double points on Enriques surfaces

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Science China Mathematics 2021年第4期64卷 665-690页

作者： Ichiro Shimada Department of Mathematics Graduate School of ScienceHiroshima UniversityHigashi-Hiroshima 739-8526Japan

We classify,up to some lattice-theoretic equivalence,all possible configurations of rational double points that can appear on a surface whose minimal resolution is a complex Enriques surface.

关键词： Enriques surface rational double points hyperbolic lattice

富含缺陷的非晶态与晶态杂化介孔钴钼纳米片高效析氧催化剂

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Science China Materials 2022年第12期65卷 3470-3478页

作者：何传生胡晓晨王佳卜令正战长宏徐冰妍李雷刚李运超黄小青 State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringXiamen University.Xiamen 361005China College of Chemistry Beijing Normal UniversityBeijing 100875China Graduate School of Integrated Sciences for Life Hiroshima UniversityHigashi-HiroshimaHiroshimaJapan

构建具有丰富缺陷且缺陷浓度可调的二维(2D)介孔金属氧化物(氢氧化物)纳米材料作为高效电催化剂仍然是一个巨大的挑战.本工作开发了一种简便有效的一锅溶剂热路线来合成由晶体相Mo_(4)O_(11)和准非晶相氢氧化钴组成的介孔Mo_(x)-Co-O杂... 详细信息

构建具有丰富缺陷且缺陷浓度可调的二维(2D)介孔金属氧化物(氢氧化物)纳米材料作为高效电催化剂仍然是一个巨大的挑战.本工作开发了一种简便有效的一锅溶剂热路线来合成由晶体相Mo_(4)O_(11)和准非晶相氢氧化钴组成的介孔Mo_(x)-Co-O杂化纳米片.由于短链有机胺在高温下的刻蚀作用,合成过程中在Mo_(x)-Co-O杂化纳米片上原位产生了大量的介孔空穴,增加了材料的电化学比表面积.此外,Mo_(x)-Co-O纳米片的尺寸、介孔和晶相/非晶相纳米片上缺陷(本工作中的氧空位)的浓度可以通过控制Mo/Co摩尔比调节.电化学测试表明,2D介孔Mo_(x)-Co-O纳米片表现出优异的析氧反应活性,其中Mo_(0.2)-Co-O纳米片表现出最高的催化活性(在1 mol L^(−1)KOH中10 mA cm^(−2)处的过电位为276 mV).研究发现,Mo_(0.2)-Co-O纳米片具有合适的d带中心和最高浓度的氧空位,这是促成其优异催化活性的两个主要因素.本工作构建具有丰富缺陷(氧空位)和合适d带中心的二维金属氧化物(氢氧化物)的晶相/非晶杂化纳米材料的方法,为设计更高效的催化剂提供了重要启发.

关键词：纳米材料介孔金属氧化物氧空位析氧反应刻蚀作用电催化剂溶剂热杂化