Regeneration of rat corpora cavernosa tissue by transplantation of CD133＋ cells derived from human bone marrow and placement of biodegradable gel sponge sheet
Regeneration of rat corpora cavernosa tissue by transplantation of CD133＋ cells derived from human bone marrow and placement of biodegradable gel sponge sheet作者机构：Department of Urology Hiroshima University Institute of Biomedical and Health Sciences Hiroshima Japan Department of Molecular Pathology Hiroshima University Institute of Biomedical and Health Sciences Hiroshima Japan
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2017年第19卷第2期
页 面：203-207页
核心收录：
主 题：alginate gel sponge sheet CD133+ cell corpora cavernosa
摘 要：The objective is to develop an easier technique for regenerating corpora cavernosa tissue through transplantation of human bone marrow-derived CD133＋ cells into a rat corpora cavernosa defect model. We excised 2 mm× 2 mm squares of the right corpora cavernosa of twenty-three 8-week-old male nude rats. AIginate gel sponge sheets supplemented with 1 × 10^4 CD133＋ cells were then placed over the excised area of nine rats. Functional and histological evaluations were carried out 8 weeks later. The mean intracavernous pressure/mean arterial pressure ratio for the nine rats （0.34258 ± 0.0831） was significantly higher than that for eight rats with only the excision （0.0580±0.0831, P = 0.0238） and similar to that for five rats for which the penis was exposed, and there was no excision （0.37228±0.1051, P = 0.8266）. Immunohistochemical analysis revealed that the nine fully treated rats had venous sinus-like structures and quantitative reverse transcription polymerase chain reaction analysis of extracts from their alginate gel sponge sheets revealed that the amounts of mRNA encoding the nerve growth factor （NGF）, and vascular endothelial growth factor （VEGF） were significantly higher than those for rats treated with alginate gel sheets without cell supplementation （NGF： P= 0.0309; VEGF： P〈 0.0001）. These findings show that transplantation of CD133＋ cells accelerates functional and histological recovery in the corpora cavernosa defect model.
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