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Evaluation of growth and reproduction as indicators of soil metal toxicity to the Collembolan, Sinella curviseta

Insect Science 2009年第1期16卷 57-63页

作者： Jie Xu Xin Ke Paul Henning Krogh Yin Wang Yong-Ming Luo Jing Song institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China department of Terrestrial Ecology National Environmental Research Institute University of Aarhus Vejlsevej 25 DK-8600 Silkeborg Denmark Soil and Environment Bioremediation research Centre Institute of Soil Sciences Chinese Academy of Sciences Nanjing China

Laboratory studies evaluated the sensitivity of Sinella curviseta Brook （Collembola： Entomobryidae） to selected heavy metals （Cu, Pb and Zn）. Survival, reproduction and growth of S. curviseta were determined in a 4-week exposure test in an agricultural soil amended with metals to concentrations of 100, 200, 400, 800, 1 600 and 3 200 mg/kg. Results showed reduction in adult survival and reproductive failure at the highest concentrations （3 200 mg/kg） of Cu and Zn. EC50reproduction values for Cu and Zn were approximately 442 and 2 760 mg/kg, respectively. Application of Pb at all levels resulted in large numbers of progeny and no significant mortality compared to controls. Adult growth rate decreased for all metal treatments compared to the controls, suggesting that metals affect S. curviseta metabolism and result in slower growth. We showed that reproduction is a slightly more sensitive parameter than growth. Since a growth test needs fewer juveniles and takes less time than a reproduction test, we conclude that the two parameters are complementary and could be used for a better ecotoxicological evaluation of contaminant levels. However, relative growth and reproduction sensitivities should be tested with more chemicals before a growth test is accepted as a faster sublethal test than a reproduction test.

关键词： Collembolan copper growth lead reproduction Sinella curviseta soil pollution toxicity testing zinc

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A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway

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Chinese Journal of Oceanology and Limnology 2009年第3期27卷 587-593页

作者：刘小金王文成王洪江张兰兰刘乐乾王跃军王春波 department of Pharmacology Medical College of Qingdao University Medical College Taishan University department of biological sciences East Tennessee State University Yellow Sea Fisheries research institute Chinese Academy of Fishery Sciences

A novel marine active polypeptide （PCF）, isolated from the gonochoric Chinese scallop, Chlamys farreri, has potential antioxidant and anti-apoptotic activity against ultraviolet irradiation. We investigated whether UVB-induced HaCaT cell apoptosis occurs via the mitochondrial pathways Apaf-1/caspase-9 and Smac/XIAP/caspase-3. We then investigated the molecular mechanisms controlling the anti-apoptotic effect of PCE Pre-treatment with PCF and caspase-9 inhibitor significantly inhibited UVB-induced apoptosis in HaCaT cells based on a DNA fragmentation assay and Hoechst 33258 staining The expression of Apaf-1 and the cleavage of procaspase-9 were dose-dependently reduced by 1.42-5.96 mmol/L PCF pretreatment in UVB-irradiated HaCaT cells. This was followed by inhibition of cleavage of procaspase-3, whose activation induced cell apoptosis. Meanwhile, PCF significantly and dose-dependently enhanced the activation of ATPase. Furthermore, we demonstrated that PCF strongly inhibited the release of Smac from the mitochondria to cytosol by reducing the degradation of XIAP dose-dependently. We conclude that the protective effect of PCF against UVB irradiation in HaCaT cells may be attributed to the inhibition of the Apaf-1/caspase-9 and Smac/XIAP/caspase-3 apoptotic signaling pathways.

关键词： polypeptide from Chlamysfarreri (PCF) UVB apoptosis Smac/XIAP Apaf-1/caspase-9

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Reclassification of Oligodon ningshaanensis Yuan, 1983(Ophidia:Colubridae) into a New Genus, Stichophanes gen. nov. with Description on Its Malacophagous Behavior

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Asian Herpetological research 2014年第3期5卷 137-149页

作者： Xiaohe WANG Kevin MESSENGER Ermi ZHAO Chaodong ZHU Chengdu institute of Biology Chinese Academy of Sciences department of biological and Environmental sciences Alabama A and M University institute of Zoology Chinese Academy of Sciences

The complete mitochondrial cytb gene and the partial nuclear c-mos gene of Oligodon ningshaanensis Yuan, 1983 were sequenced and used for reconstructing the phylogenetic relationship of this taxon amongst alethinophidian snakes. Its strong affinity to the New World subfamily Dipsadinae and the Old World species Thermophis baileyi was inferred. Hemipenial morphology found by authors conflicts with the original description and its similarity with those of the dipsadid snakes is in accordance with our molecular results. Feeding tests show that O. ningshaanensis is a malacophagous predator, which is another matchless character for this species. This behavior is described and compared with other known slug- and snail-feeding snakes. The discovery of the particular position of our subject indicates that erecting a new genus is necessary accommodate this unique species.

关键词： Stichophanes gen. nov. ningshaanensis Dipsadinae molecular phylogeny hemipenial morphology,mollusks -predator

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p53:updates on mechanisms,biology and therapy(Ⅰ)

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Journal of Molecular Cell Biology 2019年第4期11卷 185-186页

作者： Chandra S.Verma Bioinformatics institute Agency for ScienceTechnology and Research(A*STAR)Singapore 138671 department of biological sciences National University of SingaporeSingapore 117543 School of biological sciences Nanyang Technological UniversitySingapore 637551

In July 2017,the 17th International p53 Workshop was held in *** an attendanee of over 300 scientists from all over the world,the meeting was an outstanding success and many new observations about the p53 pathway were discussed for the first *** enthusiasm to gather these in sights into a form that would be available to the whole community led us and the editors of Journal of Molecular Cell Biology to design a special issue of the journal composed of articles and commentaries from some of the key contributors to the meeting.

关键词： p53 mechanisms biology and therapy

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SERKs serve as co-receptors for SYR1 to trigger systemin-mediated defense responses in tomato

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Journal of Integrative Plant Biology 2024年第10期66卷 2273-2287页

作者： Hyewon Cho Dain Seo Minsoo Kim Bo Eun Nam Soyoun Ahn Minju Kang Geul Bang Choon-Tak Kwon Youngsung Joo Eunkyoo Oh department of Life sciences Korea UniversitySeoul 02841Korea research Institute of Basic SciencesSeoul National UniversitySeoul 08826Korea School of biological sciences Seoul National UniversitySeoul 08826Korea Digital Omics research Center Ochang Institute of Biological and Environmental ScienceKorea Basic Science InstituteCheongju 28119Korea department of Smart Farm Science Kyung Hee UniversityYongin 17104Korea

Systemin, the first peptide hormone identified in plants, was initially isolated from tomato(Solanum lycopersicum) leaves. Systemin mediates local and systemic wound-induced defense responses in plants, conferring resistance to necrotrophic fungi and herbivorous insects. Systemin is recognized by the leucine-rich-repeat receptor-like kinase(LRRRLK) receptor SYSTEMIN RECEPTOR1(SYR1), but how the systemin recognition signal is transduced to intracellular signaling pathways to trigger defense responses is poorly understood. Here, we demonstrate that SERK family LRR-RLKs function as coreceptors for SYR1 to mediate systemin signal transduction in tomato. By using chemical genetic approaches coupled with engineered receptors, we revealed that the association of the cytoplasmic kinase domains of SYR1 with SERKs leads to their mutual trans-phosphorylation and the activation of SYR1, which in turn induces a wide range of defense responses. Systemin stimulates the association between SYR1 and all tomato SERKs(SlSERK1,SlSERK3A, and SlSERK3B). The resulting SYR1-SlSERK heteromeric complexes trigger the phosphorylation of TOMATO PROTEIN KINASE 1B(TPK1b), a receptor-like cytoplasmic kinase that positively regulates systemin responses. Additionally,upon association with SYR1, SlSERKs are cleaved by the Pseudomonas syringae effector HopB1, further supporting the finding that SlSERKs are activated by systemin-bound SYR1. Finally, genetic analysis using Slserk mutants showed that SlSERKs are essential for systemin-mediated defense responses. Collectively, these findings demonstrate that the systeminmediated association of SYR1 and SlSERKs activates defense responses against herbivorous insects.

关键词： LRR-RLK peptide SERK SYR1 systemin

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Continental accretion and incremental deformation in the thermochronologic evolution of the Lesser Caucasus

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Geoscience Frontiers 2019年第6期10卷 2189-2202页

作者： William Cavazza Irene Albino Ghazar Galoyan Massimiliano Zattin Silvia Cattò department of biological Geologicaland Environmental SciencesUniversity of BolognaItaly institute of Geological sciences National Academy of SciencesYerevanArmenia department of Geosciences University of PaduaItaly

Apatite fission-track analysis and thermochronologic statistical modeling of Precambrian-Oligocenc plutonic and metamorphic rocks from the Lesser Caucasus resolve two discrete cooling *** occurred during incremental crustal shortening due to obduction and continental accretion along the margins of the northern branch of the Neotethys.(1)The thermochronometric record of a Late Cretaceous(Turonian-Maastrichtian)cooling/exhumation event,coeval to widespread ophiolite obduction,is still present only in a relatively small area of the upper plate of the Amasia-Sevan-Akera(ASA)suture zone,*** suture marking the final closure of the northern Neotethys during the *** area has not been affected by significant later exhumation.(2)Rapid cooling/exhumation occurred in the Early-Middle Miocene in both the lower and upper plates of the ASA suture zone,obscuring previous thermochronologic signatures over most of the study *** contractional reactivation of the ASA suture zone occurred contemporaneously with the main phase of shortening and exhumation along the Bitlis suture zone marking the closure of the southern branch of the Neotethys and the ensuing ArabiaEurasia *** collisional stress from the Bitlis suture zone was transmitted northward across the Anatolian hinterland,which was left relatively undeformed,and focused along preexisting structural discontinuities such as the eastern Pontides and the ASA suture zone.

关键词： Lesser Caucasus Low-temperature thermochronology Apatite fission-track analysis Structural reactivation Far-field tectonics

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Identification of ISSR markers linked to flowering traits in a representative sample of Eucalyptus cladocalyx

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Journal of Forestry research 2016年第2期27卷 239-245页

作者： Rodrigo Contreras-Soto Paulina Ballesta Eduardo Ruiz Freddy Mora department of Agronomy State University of Maring department of Botany University of Concepción institute of biological sciences University of Talca

Early flowering and flower abundance have long been considered desirable traits in eucalypt breeding programs. In particular, flowers of Eucalyptus cladocalyx provide a nectar source for the production of honey in arid ecosystems. To identify inter-simple sequence repeat （ISSR） markers that are associated with early flowering and flower abundance in the southern Atacama Desert, we used a sample of 47 trees, representing five Australian prove- nances of E. cladocalyx. A unified mixed linear model （which considered the effect of genetic structure and the kinship relationship among trees） revealed that three loci were significantly associated with early flowering, which accounted for 10-16 % of the phenotypic variation, while two loci accounted for 11-13 % in flowering intensity. Locus ISO1-500 bp was associated with both flowering traits. This result is consistent with our previous findings indicating that marker-assisted selection on early flowering should have significant and positive impact on flowering intensity. The application of marker-assisted selection to identify trees that flower early and intensively may increase honey production, a resource that generates additional income for the local farmers of the southern Atacama Desert.

关键词： Eucalyptus cladocalyx ISSR Floweringtraits Marker-assisted selection

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Individual stages of bacterial dichloromethane degradation mapped by carbon and chlorine stable isotope analysis

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Journal of Environmental sciences 2019年第4期31卷 147-160页

作者： Maria L.Torgonskaya Anatoly M.Zyakun Yuri A.Trotsenko Kestutis S.Laurinavichius Steffen Kümmel Stéphane Vuilleumier Hans H.Richnow Laboratory of Radioactive Isotopes G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Laboratory of Mass Spectrometry G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences department of Isotope Biogeochemistry Helmholtz Centre for Environmental Research-UFZ Université de Strasbourg CNRS

The fractionation of carbon and chlorine stable isotopes of dichloromethane(CH_2Cl_2,DCM)upon dechlorination by cells of the aerobic methylotroph Methylobacterium extorquens DM4 and by purified DCM dehalogenases of the glutathione S-transferase family was *** effects for individual steps of the multi-stage DCM degradation process,including transfer across the cell wall from the aqueous medium to the cell cytoplasm,dehalogenase binding,and catalytic reaction,were *** observed carbon and chlorine isotope fractionation accompanying DCM consumption by cell supensions and enzymes was mainly determined by the breaking of C\Cl bonds,and not by inflow of DCM into *** isotope effects of DCM dehalogenation were initially masked in high density cultures,presumably due to inverse isotope effects of non-specific DCM oxidation under conditions of oxygen *** cofactor supply remarkably affected the correlation of variations of DCM carbon and chlorine stable isotopes(Δδ^(13)C/Δδ^(37)Cl),increasing corresponding ratio from 7.2–8.6 to 9.6–10.5 under conditions of glutathione *** suggests that enzymatic reaction of DCM with glutathione thiolate may involve stepwise breaking and making of bonds with the carbon atom of DCM,unlike the uncatalyzed reaction,which is a one-stage process,as shown by quantum-chemical modeling.

关键词： Dichloromethane(DCM) Microbial degradation Aerobic methylotrophic bacteria Dichloromethane dehalogenase Carbon and chlorine isotopic fractionation

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A high-throughput screening assay for eukaryotic elongation factor 2 kinase inhibitors

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Acta Pharmaceutica Sinica B 2016年第6期6卷 557-563页

作者： Ting Xiao Rui Liu Christopher G.Proud Ming-Wei Wang The National Center for Drug Screening and the CAS Key Laboratory of Receptor research Shanghai Institute of Materia Medica Chinese Academy of Sciences department of Pharmacy Medical College of Nanchang University Center for biological sciences Life Sciences Building University of Southampton South Australian Health and Medical research institute

Eukaryotic elongation factor 2 kinase (eEF2K) inhibitors may aid in the development of new therapeutic agents to combat cancer. Purified human eEF2K was obtained from an Escherichia coli expression system and a luminescence-based high-throughput screening (HTS) assay was developed using MH-1 peptide as the substrate. The luminescent readouts correlated with the amount of adenosine triphosphate remaining in the kinase reaction. This method was applied to a large-scale screening campaign against a diverse compound library and subsequent confirmation studies. Nine initial hits showing inhibitory activities on eEF2K were identified from 56,000 synthetic compounds during the HTS campaign, of which, five were chosen to test their effects in cancer cell lines. (C) 2016 Chinese Pharmaceutical Association and institute of Materia Medica, Chinese Academy of Medical sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND

关键词： eEF2K Inhibito high-throughput screening Luminescenc MH-1 peptide

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Light signaling and UV-B-mediated plant growth regulation^FA

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Journal of Integrative Plant Biology 2020年第9期62卷 1270-1292页

作者： Arpita Yadav Deeksha Singh Maneesh Lingwan Premachandran Yadukrishnan Shyam Kumar Masakapalli Sourav Datta department of biological sciences Indian Institute of Science Education and Research(IISER)BhopalBhopalMadhya Pradesh462066India School of Basic sciences Indian Institute of Technology(IIT)MandiKamandHimachal Pradesh175005India

Light plays an important role in plants' growth and development throughout their life cycle. Plants alter their morphological features in response to light cues of varying intensity and quality. Dedicated photoreceptors help plants to perceive light signals of different wave-lengths. Activated photoreceptors stimulate the down-stream signaling cascades that lead to extensive gene expression changes responsible for physiological and developmental responses. Proteins such as ELONGATED HYPOCOTYL5 (HY5) and CONSTITUTIVELY PHOTO-MORPHOGENIC 1 (COP1) act as important factors which modulate light-regulated gene expression, especially during seedling development. These factors function as central regulatory intermediates not only in red, far-red, and blue light pathways but also in the UV-B signaling pathway. UV-B radiation makes up only a minor fraction of sunlight, yet it imparts many positive and negative effects on plant growth. Studies on UV-B perception, signaling, and response in plants has considerably surged in recent times. Plants have developed different strat-egies to use UV-B as a developmental cue as well as to withstand high doses of UV-B radiation. Plants' re-sponses to UV-B are an integration of its cross-talks with both environmental factors and phytohormones. This review outlines the current developments in light sig-naling with a major focus on UV-B-mediated plant growth regulation.

关键词： plant Plants seedling

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