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Differentiation of Sheeppox and Goatpox Viruses by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism

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Virologica Sinica 2012年第6期27卷 353-359页

作者： Gnanavel Venkatesan Vinayagamurthy Balamurugan Revaniah Yogisharadhya Amit Kumar Veerakyathappa Bhanuprakash Division of Virology Indian Veterinary Research InstituteNainital(Distt.)Mukteswar 263138UttarakhandIndia Project Directorate on Animal Disease Monitoring and Surveillance H A FarmHebbalBangalore 560024KarnatakaIndia Indian Veterinary Research Institute H A FarmHebbalBangalore 560024KarnatakaIndia

In the present study,the partial gene sequences of P32 protein,an immunogenic envelope protein of Capripoxviruses (CaPV),were analyzed to assess the genetic relationship among sheeppox and goatpox virus isolates,and restriction enzyme specific PCR-RFLP was developed to differentiate CaPV strains.A total of six goatpox virus (GTPV) and nine sheeppox virus (SPPV) isolates of Indian origin were included in the sequence analysis of the attachment *** sequence analysis revealed a high degree of sequence identity among all the Indian SPPV and GTPV isolates at both nucleotide and amino acid *** analysis showed three distinct clusters of SPPV,GTPV and Lumpy skin disease virus (LSDV) ***,multiple sequence alignment revealed a unique change at G120A in all GTPV isolates resulting in the formation of Dra I restriction site in lieu of EcoR I,which is present in SPPV isolates *** change was unique and exploited to develop restriction enzyme specific PCR-RFLP for detection and differentiation of SPPV and GTPV *** optimized PCR-RFLP was validated using a total of fourteen (n=14) cell culture isolates and twenty two (n=22) known clinical samples of *** Restriction Enzyme specific PCR-RFLP to differentiate both species will allow a rapid differential diagnosis during CaPV outbreaks particularly in mixed flocks of sheep and goats and could be an adjunct/supportive tool for complete gene or virus genome sequencing methods.

关键词：山羊痘病毒限制性片段长度多态性聚合酶链反应 PCR-RFLP技术分化限制性内切酶基因序列系统发育分析

In vitro evaluation of binding efficacy of graded levels of asha feed at different pH conditions to absorb ochratoxin A（OA）and T-2 toxin（T-2）in broiler diets

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In vitro evaluation of binding efficacy of graded levels of ...

The 15th World Veterinary Poultry Congress

作者： Milad Manafi B.Umakantha Department of Poultry Science Veterinary CollegeHebbalBangalore560 024India Department of Poultry Science Veterinary CollegeHebbalBangalore560 024India

＜正＞Introduction Animal feed ingredients and compounded feed,by virtue of their high vital nutrients and moisture contentgenerously support the multiplication of moulds at all stages of food chain ***, harvesting,ha...

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An Effective Mannosylated Chitosan Nanoparticle DNA Vaccine for FMD Virus

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Virologica Sinica 2012年第6期27卷 373-376页

作者： Raj Kishore Nanda Bedaso Mammo Edao Irshad Ahmad Hajam Shanmugam Chandra Sekar Kondabattula Ganesh Veerakyathappa Bhanuprakash Subodh Kishore FMD Quality Control and Quality Assurance Laboratory Indian Veterinary Research InstituteH A FarmHebbalBangalore 560024KarnatakaIndia

Dear Editor,We are reporting you an effective DNA vaccine for FMD virus,and it was prepared using mannosylated chitosan (MC) nanoparticles to form the FMDVpVAC-VP1-OmpA complex *** particles were characterized for their physical properties like morphology,size and charge prior evaluating the actual vaccine delivery effect of *** immunological evaluation indicated that the 20

关键词：复合纳米粒子猪口蹄疫病毒 DNA疫苗壳聚糖糖化 ELISA法物理性质中和抗体

Synthesis, Characterization and Anti-Angiogenic Effects of Novel 5-Amino Pyrazole Derivatives on Ehrlich Ascites Tumor [EAT] Cells in-Vivo

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Journal of Cancer Therapy 2010年第1期1卷 1-9页

作者： H. Raju S. Chandrappa M. K. Ramakrishna T. S. Nagamani H. Ananda S. M. Byregowda K. S. Rangappa Department of Studies in Chemistry University of Mysore Manasagangotri India Institute of Animal Health and Veterinary Biologicals Hebbal India

In search of synthetic chemotherapeutic substances capable of inhibiting, retarding, or reversing the process of multi-stage carcinogenesis, we synthesized a series of novel 5-amino pyrazole derivatives 11(a-h) by a nucleophilic substitution reaction and characterized by 1H nuclear magnetic resonance (NMR), liquid chromatography mass spectrometry (LC/MS), Fourier-transform infrared (FTIR), and elemental analysis. These novel compounds were evaluated for their efficacy in inhibiting Ehrlich ascites tumor [EAT] cells in-vivo. In the present study we designed, synthesized, characterized and investigate the anti-angiogenic effects of these compounds, on Ehrlich ascites tumor [EAT] cells in-vivo. The compounds were subsequently tested for their ability to inhibit neovascularisation in chorio allantoin membrane (CAM) model. From the Structure Activity Relationship (SAR) studies, it reveals that, the substitution at N-terminal in pyrazole ring plays key role in the antitumor and anti-angiogenic effects.

关键词： 1H-Pyrazol Aryl Isothiocyanates Ehrlich Ascites Tumor [EAT] Cells Anti-Angiogenesis

高级斑脱土(Ashafeed)在减轻T-2毒素、黄曲霉毒素毒害中的作用

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高级斑脱土(Ashafeed)在减轻T-2毒素、黄曲霉毒素毒害中的作用

第15届世界禽病大会、中国畜牧兽医学会2007年学术年会

作者： Milad Manafi B.Umakantha N.Pirany 王宏俊 Department of Poultry Science Veterinary College hebbal Bangalore 560 024 India Department of Poultry Science Veterinary College hebbal Bangalore 560 024 India Department of Animal Science Agriculture Faculty Tabriz University Tabriz Iran

前言：鸡只大量采食被毒枝菌素污染的饲料后,常会引起生长受阻、免疫力下降,最终导致大量的经济损失。Ashafeed 是一种天然的斑脱土,它的表面积很大,因而具有较强的吸附能力。利用这一特点,Ashafeed

关键词： T-2 Ashafeed

Non-Starch polysacharids degrading enzymes influence the health and economic of production in layer hen’s fed on diets containing high fiber protein sources

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Non-Starch polysacharids degrading enzymes influence the hea...

The 15th World Veterinary Poultry Congress

作者： G.Paradise G.Devegowda H.Khosravinia Department of Poultry Science Veterinary CollegeUASHebbalBangalore-560 032.INDIA. Department of Poultry Science Veterinary CollegeUASHebbalBangalore-560 032.INDIA. Dept.of Technology of Animal Products Agriculture FacultyLorestan UniversityP.B.465Khoramabad-68135Iran

＜正＞Sunflower meal（SFM）and deoiled rice bran（DORB）,are two agro-industrial protein sources with high levels of crude fiber which their annual production exceeds 1 and 15 million tones,respectively,in India.A stu...

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非淀粉多糖降解酶对饲喂含高纤维蛋白原料日粮的产蛋母鸡健康状况和经济效益的影响

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非淀粉多糖降解酶对饲喂含高纤维蛋白原料日粮的产蛋母鸡健康状况...

第15届世界禽病大会、中国畜牧兽医学会2007年学术年会

作者： G.Paradise G.Devegowda H.Khosravinia 戴亚斌 Department of Poultry Science Veterinary College UAS hebbal Bangalore-560 032.India Department of Poultry Science Veterinary College UAS hebbal Bangalore-560 032.India Dept.of Technology of Animal Products Agriculture Faculty Lorestan University P.B.465 Khoramabad-68135 Iran

向日葵粉(SFM)和脱脂稻麸(DORB)是农产品加工业中的2种高纤维蛋白原料,在印度两者的年产量分别超过100和1500万吨。本研究选用288只产蛋量和体重相似的46周龄 BV300来航鸡,以含50％ SFM

关键词：产蛋性能向日葵粉脱脂稻麸非淀粉多糖降解酶

Understanding the Effect of Zinc Oxide(ZnO)With Carbon Black Coupling Agent on Physico-Mechanical Properties in Natural Rubber Matrix

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Organic Polymer Material Research 2022年第2期4卷 15-25页

作者： Koushik Pal Koushik Banerjee Soumya Ghosh Chowdhury Sanjay Kumar Bhattacharyya Rabindra Mukhopadhyay Hari Shankar Singhania Elastomer and Tyre research Institute Plot No.437Hebbal Industrial AreaMysoreKarnataka570016India

Natural rubber(NR)is not only the main compounding ingredient used to make the majority of components of tires as well as other rubber products,as it plays a significant role in ensuring that they operate well and complies with environmental *** applications of NR products are lim-ited to high temperatures due to the revision tendency of NR *** address these issues,the potential engagement of a carbon black(CB)coupling agent(CA)in the presence of metal oxide *** Oxide(ZnO)was investigated in an NR-based *** CA has dual functionality on physicomechanical *** has the ability to reduce hysteresis loss as well as improve anti-reversion properties and these properties thorough-ly depend on the presence of *** ZnO was added to the master formulation,a 65%improvement in reversion properties was *** the other hand,while ZnO fully transferred to the final formulation,bound rubber(BR)content increased by 19%,the difference in storage modulus(ΔG’)is reduced by 22%,cure rate index(CRI)improved by 14%,loss tangent(tanδ)reduced by 18%and slightly improve in elongation at break compared to control ***-gravimetric analysis(TGA)was engaged to understand the thermal stability and degree of purity of CA.A differential Scanning Calorimeter(DSC)was used to detect the phase tran-sition of *** Transform Infrared Spectrum(FTIR)was adopted to detect the presence of carboxyl and amine groups in the CA *** effect,BR content and Transmission Electron Microscope(TEM)were em-ployed to investigate the micro-level dispersion of CB in the natural rubber(NR)matrix.

关键词： Carbon black Natural rubber Carbon black coupling agent Anti-reversion properties

Evaluation of Efficacy of Stabilizers on the Thermostability of Live Attenuated Thermo-adapted Peste des petits ruminants Vaccines

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Virologica Sinica 2011年第5期26卷 324-337页

作者： Thachamvally Riyesh Vinayagamurthy Balamurugan Arnab Sen Veerakyathappa Bhanuprakash Gnanavel Venkatesan Vinita Yadav Raj Kumar Singh Division of Virology Indian Veterinary Research InstituteCampus Mukteswar 263138Nainital DistrictUttarakhandIndia Veterinary Type cultures Centre National Research Centre on EquinesSirsa RoadHisar 125001HaryanaIndia Project Directorate on Animal Disease Monitoring And Surveillance (PD-ADMAS) HebbalBangalore 560024India National Research Centre on Equines Sirsa RoadHisar 125001HaryanaIndia

In this study,thermo-adapted (Ta) PPR vaccines were assessed for their stability at 25,37,40,42 and 45℃ in lyophilized form using two extrinsic stabilizers {lactalbumin hydrolysate-sucrose (LS) and stabilizer E} and in reconstituted form with the diluents (1 mol/L MgSO4 or 0.85% NaCl).The lyophilized vaccines showed an expiry period of 24-26 days at 25℃,7-8 days at 37℃ and 3-4 days at 40℃.LS stabilizer was superior at 42℃ with a shelf-life of 44 h,whereas in stabilizer E,a 40 h shelf-life with a comparable half-life was *** 45℃,the half-life in stabilizer E was better than LS and lasted for 1 ***,the reconstituted vaccine maintained the titre for 48 h both at 4℃ and 25℃ and for 24-30 h at 37℃.As both the stabilizers performed equally well with regard to shelf-life and half-life,the present study suggests LS as stabilizer as a choice for lyophilization with 0.85% NaCl diluent,because it has better performance at higher *** Ta vaccines can be used as alternatives to existing vaccines for the control of the disease in tropical countries as they are effective in avoiding vaccination failure due to the breakdown in cold-chain maintenance,as this vaccine is considerably more stable at ambient temperatures.

关键词：冻干疫苗稳定剂热适应疗效评价 DES 反刍减毒重组疫苗