Evaluation of Efficacy of Stabilizers on the Thermostability of Live Attenuated Thermo-adapted Peste des petits ruminants Vaccines

作     者：Thachamvally Riyesh Vinayagamurthy Balamurugan Arnab Sen Veerakyathappa Bhanuprakash Gnanavel Venkatesan Vinita Yadav Raj Kumar Singh 

作者机构：Division of VirologyIndian Veterinary Research InstituteCampus Mukteswar 263138Nainital DistrictUttarakhandIndia Veterinary Type cultures CentreNational Research Centre on EquinesSirsa RoadHisar 125001HaryanaIndia Project Directorate on Animal Disease Monitoring And Surveillance (PD-ADMAS)HebbalBangalore 560024India National Research Centre on EquinesSirsa RoadHisar 125001HaryanaIndia 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2011年第26卷第5期

页      面：324-337页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 090601[农学-基础兽医学] 08[工学] 09[农学] 0906[农学-兽医学] 083203[工学-农产品加工及贮藏工程] 

基　　金：Supported by grants from Indian Council of Agricultural Research Ministry of Agriculture Government of India New Delhi under the Ad-hoc Scheme(F.No.11-3/2007-GA-II and1-1/2007-ASR-IV) 

主　　题：PPR Thermo-adapted Vaccines Stabilizers Diluents Thermo-stability 

摘      要：In this study, thermo-adapted （Ta） PPR vaccines were assessed for their stability at 25, 37, 40, 42 and 45℃ in lyophilized form using two extrinsic stabilizers {lactalbumin hydrolysate-sucrose （LS） and stabilizer E} and in reconstituted form with the diluents （1 mol/L MgSO4 or 0.85% NaC1）. The lyophilized vaccines showed an expiry period of 24-26 days at 25℃, 7-8 days at 37℃ and 3-4 days at 40℃. LS stabilizer was superior at 42℃ with a shelf-life of 44 h, whereas in stabilizer E, a 40 h shelf-life with a comparable half-life was observed. At 45 ℃, the half-life in stabilizer E was better than LS and lasted for 1 day. Furthermore, the reconstituted vaccine maintained the titre for 48 h both at 4℃ and 25℃ and for 24-30 h at 37℃. As both the stabilizers performed equally well with regard to shelf-life and half-life, the present study suggests LS as stabilizer as a choice for lyophilization with 0.85% NaCldiluent, because it has better performance at higher temperature. These Ta vaccines can be used as alternatives to existing vaccines for the control of the disease in tropical countries as they are effective in avoiding vaccination failure due to the breakdown in cold-chain maintenance, as this vaccine is considerably more stable at ambient temperatures.