检索结果-南通市图书馆

Flux Balance Analysis Reveals Potential Anti-HIV-1 Metabolic Targets

在线全文

学校读者我要写书评

暂无评论

Infectious Diseases & Immunity 2024年第2期4卷 61-68页

作者： Runpeng Han Fei Luo Haisheng Yu Yajun Yan Yan Gong Conghua Xie Liang Cheng Department of Radiation and Medical Oncology Medical Research InstituteZhongnan Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China Frontier Science Center for Immunology and Metabolism Zhongnan Hospital of Wuhan UniversityState Key Laboratory of VirologyWuhan UniversityWuhan 430071China Guangzhou Eighth People's Hospital Guangzhou Medical UniversityGuangzhou 510440China Department of Infectious Diseases Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Biological Repositories Zhongnan Hospital of Wuhan UniversityWuhan 430071China Wuhan research Center for Infectious Diseases and Cancer Chinese Academy of Medical SciencesWuhan 430071China

Background:Human immunodeficiency virus type 1(HIV-1)remains a persistent global health challenge.Therefore,a continuous exploration of novel therapeutic strategies is essential.A comprehensive understanding of how HIV-1 utilizes the cellular metabolism machinery for replication can provide insights into new therapeutic approaches.Methods:In this study,we performed a flux balance analysis using a genome-scale metabolic model(GEM)integrated with an HIV-1 viral biomass objective function to identify potential targets for anti–HIV-1 interventions.We generated a GEM by integrating an HIV-1 production reaction into CD4+T cells and optimized for both host and virus optimal states as objective functions to depict metabolic profiles of cells in the status for optimal host biomass maintenance or for optimal HIV-1 virion production.Differential analysis was used to predict biochemical reactions altered optimal for HIV-1 production.In addition,we conducted in silico simulations involving gene and reaction knock-outs to identify potential anti–HIV-1 targets,which were subsequently validated by human phytohemagglutinin(PHA)blasts infected with HIV-1.Results:Differential analysis identified several altered biochemical reactions,including increased lysine uptake and oxidative phosphorylation(OXPHOS)activities in the virus optima compared with the host optima.In silico gene and reaction knock-out simulations revealed de novo pyrimidine synthesis,and OXPHOS could serve as potential anti–HIV-1 metabolic targets.In vitro assay confirmed that targeting OXPHOS using metformin could suppress the replication of HIV-1 by 56.6%(385.4±67.5 pg/mL in the metformintreated group vs.888.4±32.3 pg/mL in the control group,P<0.001).Conclusion:Our integrated host-virus genome-scale metabolic study provides insights on potential targets(OXPHOS)for anti-HIV therapies.

关键词： HIV-1 Flux balance analysis Genome-scale metabolic models Viral biomass objective function Therapeutic target

The neurovascular unit-on-a-chip:modeling ischemic stroke to stem cell therapy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration research 2024年第7期19卷 1431-1432页

作者： Seonghun Kim Minjun Kim Gerald A.Grant Wonjae Lee Department of Neurosurgery Duke University School of MedicineDurhamNCUSA Department of Neurobiology Duke University School of MedicineDurhamNCUSA Duke institute for Brain Sciences Duke University School of MedicineDurhamNCUSA Duke Cancer institute Duke University School of MedicineDurhamNCUSA

The neurovascular unit and stem cell therapy in ischemic stroke:Ischemic stroke,accounts for approximately 85% of all stroke incidents and is a major global health burden.It is the leading cause of disability and death worldwide,posing immense societal and economic challenges due to the long-term care required for stro ke survivors and the significant healthcare costs associated with its treatment and management(Amarenco et al.,2009).

关键词： death unit

Multifunctional RGD coated a single-atom iron nanozyme:A highly selective approach to inducing ferroptosis and enhancing immunotherapy for pancreatic cancer

在线全文

学校读者我要写书评

暂无评论

Nano research 2024年第6期17卷 5469-5478页

作者： Haoqi Pan Xu Chen Mingming Xiao He Xu Jiansheng Guo Zhiyi Lu Dong Cen Xianjun Yu Si Shi Department of Pancreatic Surgery Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Shanghai Pancreatic Cancer institute Shanghai 200032China Pancreatic Cancer institute Fudan UniversityShanghai 200032China Key Laboratory of Advanced Fuel Cells and Electrolyzers Technology of Zhejiang Province Qianwan Institute of CNITECHNingbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China Department of Pathology of Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou 310016China Key Laboratory of Laparoscopic Technology of Zhejiang Province Department of General SurgerySir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China

Nanozyme is a new promising approach to cancer therapy for its ability to induce ferroptosis by activating H_(2)O_(2)via a traditional radical pathway and enhance cancer immunotherapy.However,short half-life period of hydroxyl radical(·OH)results in unsatisfied effectiveness.Herein,we synthesized a single-atom iron nanozyme(Fe-SAzyme),which can activate H_(2)O_(2)via a non-radical pathway to generate Fe-based reactive oxygen species(ROS)(O=FeO_(3)=O)for promoting the ferroptosis of pancreatic cancer cells.This Fe-SAzyme could be specifically phagocytosed by pancreatic cancer cells,increasing ROS levels and inhibiting glutathione(GSH)synthesis,which activates ferroptosis.Tumor magnetic resonance imaging(MRI)showed decreased T2 signal after intravenous injection of RGD@Fe-AC(AC=activated carbon).Moreover,RGD@Fe-AC promoted dendritic cell(DC)maturation,overcame Treg-mediated immunosuppression,activated T cells to trigger adaptive immune responses,and enhanced the efficacy ofα-PD-L1 immunotherapy.Our research demonstrated that RGD@Fe-AC provided a straightforward,easily implemented,and selective approach for pancreatic cancer treatment and immunotherapy.

关键词： single-atom iron nanozyme pancreatic cancer immunotherapy ferroptosis

Oligodendrocytes in central nervous system diseases:the effect of cytokine regulation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration research 2024年第10期19卷 2132-2143页

作者： Chengfu Zhang Mengsheng Qiu Hui Fu Center for Cognition and Brain Disorders The Affiliated Hospital of Hangzhou Normal UniversityHangzhouZhejiang ProvinceChina Department of Psychiatry National Clinical Research Center for Mental Disordersand National Center for Mental DisordersThe Second Xiangya Hospital of Central South UniversityChangshaHunan ProvinceChina institute of Life Sciences Key Laboratory of Organ Development and Regeneration of Zhejiang ProvinceCollege of Life and Environment SciencesHangzhou Normal UniversityHangzhouZhejiang ProvinceChina Key Laboratory of Aging and Cancer Biology of Zhejiang Province School of Basic Medical SciencesHangzhou Normal UniversityHangzhouZhejiang ProvinceChina

Cytokines including tumor necrosis factor, interleukins, interferons, and chemokines are abundantly produced in various diseases. As pleiotropic factors, cytokines are involved in nearly every aspect of cellular functions such as migration, survival, proliferation, and differentiation. Oligodendrocytes are the myelin-forming cells in the central nervous system and play critical roles in the conduction of action potentials, supply of metabolic components for axons, and other functions. Emerging evidence suggests that both oligodendrocytes and oligodendrocyte precursor cells are vulnerable to cytokines released under pathological conditions. This review mainly summarizes the effects of cytokines on oligodendrocyte lineage cells in central nervous system diseases. A comprehensive understanding of the effects of cytokines on oligodendrocyte lineage cells contributes to our understanding of central nervous system diseases and offers insights into treatment strategies.

关键词： astrocyte central nervous system disease CXC chemokine cytokine interferonγ interleukin microglia oligodendrocyte oligodendrocyte precursor cell tumor necrosis factorα

Assessment and Optimization of Explainable Machine Learning Models Applied to Transcriptomic Data

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2022年第5期20卷 899-911页

作者： Yongbing Zhao Jinfeng Shao Yan W.Asmann Department of Quantitative health Sciences Mayo ClinicJacksonvilleFL 32224USA The Laboratory of Malaria and Vector research National Institute of Allergy and Infectious DiseasesNational Institutes of HealthRockvilleMD 20852USA

Explainable artificial intelligence aims to interpret how machine learning models make decisions,and many model explainers have been developed in the computer vision field.However,understanding of the applicability of these model explainers to biological data is still lacking.In this study,we comprehensively evaluated multiple explainers by interpreting pre-trained models for predicting tissue types from transcriptomic data and by identifying the top contributing genes from each sample with the greatest impacts on model prediction.To improve the reproducibility and interpretability of results generated by model explainers,we proposed a series of optimization strategies for each explainer on two different model architectures of multilayer perceptron(MLP)and convolutional neural network(CNN).We observed three groups of explainer and model architecture combinations with high reproducibility.Group II,which contains three model explainers on aggregated MLP models,identified top contributing genes in different tissues that exhibited tissue-specific manifestation and were potential cancer biomarkers.In summary,our work provides novel insights and guidance for exploring biological mechanisms using explainable machine learning models.

关键词： Machine learning Model interpretability Gene expression Marker gene Omics data mining

Flora, Structure and Carbon Sequestration of Vegetation in the Southeast of the Mono Biosphere Reserve in Togo Amidst Environmental Challenges

在线全文

学校读者我要写书评

暂无评论

Open Journal of Forestry 2024年第2期14卷 155-181页

作者： Gbétey Kokouvi Akpamou Kossi Adjossou Hodabalo Egbelou Komlanvi Katche Akoete Kossi Hounkpati Kouami Kokou Faculty of Science-Forest research Laboratory (FRL) University of Lome Lome Togo Ministry of the Environment and Forest Resources Lom&#233 Togo National Agricultural Training institute (INFA) of Tove Kpalime Togo

In most Sub-Saharan African countries such as Togo, people’s heavy dependence on ecosystem services is a major factor in accelerating the degradation of natural resources, which are already suffering as a result of climatic factors. This study was initiated to contribute to the sustainable and rational management of forest resources in the south-east of the Mono Biosphere Reserve in Togo. It specifically aims to identify and characterize the flora of the residual forest ecosystems within the reserve through their specific diversity, demographic structure, and carbon sequestration potential. The study was carried out in the forest ecosystems of Avévé. The methodology used was based on the analysis of phytosociological, forestry, ecological, and regeneration inventory data. Overall, the study revealed that the RBMT still has floristically viable habitats, despite the anthropogenic pressures it is subject to revealed a floristic diversity of 160 plant species divided into 52 families and 135 genera. The most represented families are Rubiaceae (29.09%), followed by Fabaceae (27.94%). The most represented species are Mitragyna inermis (Willd.) Kuntze (24.38%), Lecaniodiscus cupanioides Planch. Ex Benth (X) and Lonchocarpus sericeus (Poir.) Kunth (10.93%). The high presence of Mitragyna inermis observed in all the ecological groups identified makes it the characteristic species of the flooded marshy areas of southeast Togo and contributes to the resilience of the ecosystems and populations in the study area. The Shannon index for the formation groups varies between (3.03 and 5.16) bits. Pielou’s equitability varies between (0.43 and 0.63) bits. The overall average density is estimated at 210 stems/ha, with an average diameter of 25.57 ± 21.77 m and an average height of 7.93 ± 3.83 m. The adjustment of the diameter classes of the plant groups to the Weibull distribution gave an “inverted J” shape with coefficient values of less than 1 overall, reflecting the existence of multispecific or uneven-aged stands. Assessment o

关键词： Biodiversity Demographic Structure Carbon Biosphere Reserve Forest Patches Togo

Nonlinear optical microscopy for skin in vivo:Basics,development and applications

维普期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Journal of Innovative Optical health Sciences 2023年第1期16卷 95-113页

作者： Jianhua Zhao Yuan Zhao Zhenguo Wu Yunxian Tian Haishan Zeng Integrative Oncology Department–Imaging Unit BC Cancer research institute VancouverBCCanada Department of Dermatology and Skin Science University of British Columbia and Vancouver Coastal Health Research Institute VancouverBCCanada

Multi-photon microscopy(MPM)and coherent anti-Stokes Raman scattering(CARS)are two advanced nonlinear optical imaging techniques,which provide complementary information and have great potential in combination for noninvasive in vrito biomedical applications.This paper provides a detailed discussion of the basics,development and applications of these technologies for in vrivo skin research,covering the following topics:The principle and advantage of MPM and CARS,instrumentation development for in vino applications,MPM and CARS of normal skin,application of MPM and CARS in skin cancer and disease diagnosis;application of MPM in skin disease intervention,ie.,imaging guided two-photon photothermolysis.

关键词： Nonlinear microscopy multiphoton microscopy coherent anti-Stokes Raman scattering microscopy skin skin cancer multiphoton therapy

The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2024年第1期12卷 295-300页

作者： Liping Shen Lili Zhang Changbin Yin Xiaowan Xu Yangyang Liu Kuocheng Shen He Wu Zhiwen Sun Ke Wang Zhonghu He Xueyong Zhang Chenyang Hao Jian Hou Aoyue Bi Xuebo Zhao Daxing Xu Botao Ye Xuchang Yu Ziying Wang Danni Liu Yuanfeng Hao Fei Lu Zifeng Guo Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China China National Botanical Garden Beijing 100093China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China institute of Crop Sciences Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China University of Chinese Academy of Sciences Beijing 100049China International Maize and Wheat Improvement Center(CIMMYT)China Office c/o CAASBeijing 100081China CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS) Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The effects of TaSus1 on grain number per spike(GNS)also are largely unknown.Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike(FSN).In the present study,we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing.The triple mutants displayed lower FSN,GNS,grain number per spikelet(GNST),and TGW than wild-type plants.In 306 hexaploid wheat accessions,two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS.Introgression of the two alleles into a wheat genetic background confirmed their effects.The alleles differed in geographical distribution among the accessions.

关键词： Domestication selection Fertile spikelet number per spike(FSN) Geographical differentiation Grain number per spike(GNS) TaSus1

Numerical simulation on sand sedimentation and erosion characteristics around HDPE sheet sand barrier under different wind angles

在线全文

学校读者我要写书评

暂无评论

Journal of Mountain Science 2024年第2期21卷 538-554页

作者： ZHANG Kai ZHANG Peili ZHANG Hailong TIAN Jianjin WANG Zhenghui XIAO Jianhua College of Civil Engineering Lanzhou Jiaotong UniversityLanzhou 730000China Key Laboratory of Desert and Desertification Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China

For the safety of railroad operations,sand barriers are utilized to mitigate wind-sand disaster effects.These disasters,characterized by multi-directional wind patterns,result in diverse angles among the barriers.In this study,using numerical simulations,we examined the behavior of High Density Polyethylene(HDPE)sheet sand barriers under different wind angles,focusing on flow field distribution,windproof efficiency,and sedimentation erosion dynamics.This study discovered that at a steady wind speed,airflow velocity varies as the angle between the airflow and the HDPE barrier changes.Specifically,a 90°angle results in the widest low-speed airflow area on the barrier’s downwind side.If the airflow is not perpendicular to the barrier,it prompts a lateral airflow movement which decreases as the angle expands.The windproof efficiency correlates directly with this angle but inversely with the wind’s speed.Notably,with a wind angle of 90°,wind speed drops by 81%.The minimum wind speed is found at 5.1H(the sand barrier height)on the barrier’s downwind side.As the angle grows,the barrier’s windproof efficiency improves,extending its protective reach.Sedimentation is most prominent on the barrier’s downwind side,as the wind angle shifts from 30°to 90°,the sand sedimentation area on the barrier’s downwind side enlarges by 14.8H.As the angle grows,sedimentation intensifies,eventually overtakes the forward erosion and enlarges the sedimentation area.

关键词： Multi-wind direction HDPE sheet sand barrier Numerical simulation Windproof efficiency Sedimentation erosion