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Detection,isolation,and characterization of a novel impurity from several folic acid products

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第7期23卷 597-606页

作者： Qirong SHEN Quan HE Yuanjiang PAN Cuirong SUN co.lege of pharmaceutical Sciences Zhejiang UniversityHangzhou310058China Department of Chemistry Zhejiang UniversityHangzhou310027China Hangzhou Qianyuan Pollen Pharmaceutical Co. Ltd.Hangzhou310018China

Folic acid belongs to the group of water-soluble B vitamins and naturally exists in multiple forms in a wide variety of foods such as legumes,vegetables,liver,and milk(Iyer and Tomar,2009;Lyon et al.,2020).It is involved in many biochemical reactions critical for cell division,such as purine and pyrimidine biosynthesis,DNA/RNA biosynthesis,and amino acid metabolism(Iyer and Tomar,2009).Mammals cannot synthesize folic acid and thus they must acquire it from food.Although folic acid is ubiquitous in foods.

关键词： belongs characterization synthesize

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Molecular breeding of water lily: engineering co.d stress tolerance into tropical water lily

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Horticulture Research 2018年第1期5卷 91-101页

作者： Cuiwei Yu Guirong Qiao Wenmin Qiu Dongbei Yu Shirong Zhou Yan Shen Guanchun Yu Jing Jiang Xiaojiao Han Mingying Liu Liangsheng Zhang Fei Chen Yuchu Chen Renying Zhuo State Key Laboratory of Tree Genetics and Breeding Chinese Academy of Forestry100091BeijingChina Zhejiang Humanities Landscape co. Ltd.Hangzhou Tianjing Aquatic Botanical Garden310000HangzhouZhejiangChina Key Laboratory of Tree Breeding of Zhejiang Province The Research Institute of Subtropical forestryChinese Academy of Forestry311400HangzhouZhejiangChina State Key Laboratory of Eco.ogical Pest co.trol for Fujian and Taiwan Crops Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry University350002FuzhouChina

Water lilies(order Nymphaeales)are rich in both eco.omic and cultural values.They grow into aquatic herbs,and are divided into two eco.ogical types:tropical and hardy.Although tropical water lilies have more ornamental and medicinal values co.pared to the hardy water lily,the study and utilization of tropical water lilies in both landscaping and pharmaceutical use is greatly hindered due to their limited planting area.Tropical water lilies cannot survive the winter in areas beyond 24.3°N latitude.Here,the transgenic pipeline through the pollen-tube pathway was generated for water lily for the first time.To improve co.d stress tolerance of tropical water lilies,a gene enco.ing choline oxidase(co.A)driven by a co.d stress-inducible promoter was transformed into a tropical water lily through the pollen-tube transformation.Six independent transgenic lines were tested for survival rate during two winter seasons from 2015 to 2017 in hangzhou(30.3°N latitude).PCR and southern blot detection revealed that the co.A gene had been integrated into the genome.Reverse transcription PCR showed that co.A gene was induced after co.d stress treatment,and further quantitative real-time PCR revealed different expressions among six 4 lines and line 3 had the highest expression.Multiple physiological experiments showed that after co.d stress treatment,both the co.ductivity and malondialdehyde(MDA)levels from transgenic plants were significantly lower than those of non-transgenic plants,whereas the co.tent of betaine and the activity of superoxide dismutase,catalase,and peroxidase were higher than those from non-transgenic plants.These results suggest that expression of exogenous co.A gene significantly improved the co.d stress tolerance of tropical water lilies through a wide range of physiological alterations.Our results currently expanded a six-latitude cultivating area of the tropical water lilies.These results not only illuminate the bright future for water lily breeding but will also facilitate the functional genomic studies.

关键词： tropical breeding stress

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