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A homeodomain-leucine zipper I transcription factor, MeHDZ14,regulates internode elongation and leaf rolling in cassava(Manihot esculenta Crantz)

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The Crop Journal 2023年第5期11卷 1419-1430页

作者： Xiaoling Yu Xin Guo Pingjuan Zhao Shuxia Li Liangping Zou Wenbin Li Ziyin Xu Ming Peng Mengbin Ruan National key laboratory for tropical Crop Breeding Sanya 572025HainanChina key laboratory of biology and Genetic Resources of tropical crops Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101HainanChina key laboratory for biology and Genetic Resources of tropical crops of hainan Province Hainan Institute for Tropical Agricultural ResourcesHaikou 571101HainanChina Sanya research institute Chinese Academy of Tropical Agricultural SciencesSanya 572025HainanChina College of tropical crops Hainan UniversityHaikou 570228HainanChina

Drought stress impairs plant growth and other physiological functions. MeHDZ14, a homeodomainleucine zipper I transcription factor, is strongly induced by drought stress in various cassava ***, the role of MeHDZ14 in cassava growth regulation has remained unclear. Here we report that MeHDZ14 affected plant height, such that a dwarf phenotype and altered internode elongation were observed in transgenic cassava lines. MeHDZ14 was found to negatively regulate the biosynthesis of lignin. Its overexpression resulted in abaxially rolled leaves. The morphogenesis of leaf epidermal cells was inhibited by overexpression of MeHDZ14, with decreased auxin and gibberellin and increased cytokinin contents. MeHDZ14 was found to regulate many drought-responsive genes, including genes involved in cell wall synthesis and expansion. MeHDZ14 bound to the promoter of caffeic acid 3-Omethyltransferase 1(MeCOMT1), acting as a transcriptional repressor of genes involved in cell wall development. MeHDZ14 appears to act as a negative regulator of internode elongation and epidermal cell morphogenesis during cassava leaf development.

关键词： HD-Zip transcription factor Drought Internode elongation Leaf rolling Cassava

MaDREB1F confers cold and drought stress resistance through common regulation of hormone synthesis and protectant metabolite contents in banana

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Horticulture research 2023年第2期10卷 227-240页

作者： Yi Xu Wei Hu Shun Song Xiaoxue Ye Zehong Ding Juhua Liu Zhuo Wang Jingyang Li Xiaowan Hou Biyu Xu Zhiqiang Jin Haikou Experimental Station Key Laboratory of Genetic Improvement of BananasSanya Research InstituteState Key Laboratory of Biological Breeding for Tropical CropsInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHainanChina hainan key laboratory for Protection and Utilization of tropical Bioresources Hainan Institute for Tropical Agricultural ResourcesChinese Academy of Tropical Agricultural SciencesHainanChina hainan Yazhou Bay Seed laboratory HainanChina hainan key laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions Key Laboratory of Biology and Genetic Resources of Tropical CropsInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHainanChina key laboratory of hainan Province for Postharvest Physiology and Technology of tropical Horticultural Products South Subtropical Crops Research InstituteChinese Academy of Tropical Agricultural SciencesGuangdongChina

Adverse environmental factors severely affect crop *** crop resistance to multiple stressors is an important breeding *** CBFs/DREB1s extensively participate in plant resistance to abiotic stress,the common mechanism underlying CBFs/DREB1s that mediate resistance to multiple stressors remains ***,we show the common mechanism for MaDREB1F conferring cold and drought stress resistance in ***1F encodes a dehydration-responsive element binding protein(DREB)transcription factor with nuclear localization and transcriptional ***1F expression is significantly induced after cold,osmotic,and salt ***1F overexpression increases banana resistance to cold and drought stress by common modulation of the protectant metabolite levels of soluble sugar and proline,activating the antioxidant system,and promoting jasmonate and ethylene *** analysis shows that MaDREB1F activates or alleviates the repression of jasmonate and ethylene biosynthetic genes under cold and drought ***,MaDREB1F directly activates the promoter activities of MaAOC4 and MaACO_(2)0 for jasmonate and ethylene syntheses,respectively,under cold and drought ***1F also targets the MaERF11 promoter to activate MaACO_(2)0 expression for ethylene synthesis under drought ***,our findings offer new insight into the common mechanism underlying CBF/DREB1-mediated cold and drought stress resistance,which has substantial implications for engineering cold-and drought-tolerant crops.

关键词： synthesis sugar finding

Identification and characterization of MeERF genes and their targets in pathogen response by cassava(Manihot esculenta)

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The Crop Journal 2021年第5期9卷 1145-1153页

作者： Yuhui Hong Yong Xiao Na Song Shousong Zhu Rui Zhao Ke Li Mengting Geng Xiaohui Yu Honggang Wang Wei Xia Yinhua Chen hainan key laboratory for Sustainable Utilization of tropical Bioresource College of Tropical CropsHainan UniversityHaikou 570228HainanChina coconut research institute Chinese Academy of Tropical Agricultural SciencesWenchang 571339HainanChina School of Bioengineering Dalian University of TechnologyDalian 116024LiaoningChina

Cassava,Manihot esculenta Crantz (Me),is a major dietary source of calories for over 700 million people in tropical *** production of cassava is constantly threatened by cassava bacterial blight (CBB),caused by Xanthomonas axonopodis *** (Xam).The gene resources for CBB-resistant breeding of cassava are *** model plant species,ethylene response factors play important roles in response to pathogen *** this study,cassava ethylene response factors (MeERFs) were identified and characterized as the first step in studying their potential for CBB-resistant breeding of *** the cassava genome 155 MeERFs were identified,of which 23 were induced by Xam *** promoter regions of204 genes harbored GCC-box that had the potential to interact with *** 37 transcriptomes derived from Xam infection treatment,four gene co-expression modules for the MeERFs and GCC-box containing genes were *** MeERFs were associated with two GCC-box containing genes:transcription initiation factor TFIIE subunit beta (MeTFIIE),and histone-lysine N-methyltransferase ASHR1 (MeASHR1).Dual-luciferase reporter assays showed that MeERF10 and MeERF58 positively regulated Me TFIIE;MeERF137 negatively regulated Me TFIIE;MeERF10 and MeERF137 positively regulated Me ASHR1;and MeERF35 negatively regulated Me *** four MeERFs may mediate pathogen response by regulating the expression of the two GCC-box containing genes.

关键词： Cassava Ethylene response factor Expression profile Co-expression analysis Pathogen

A novel long non-coding RNA, DIR, increases drought tolerance in cassava by modifying stress-related gene expression

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Journal of Integrative Agriculture 2022年第9期21卷 2588-2602页

作者： DONG Shi-man XIAO Liang LI Zhi-bo SHEN Jie YAN Hua-bing LI Shu-xia LIAO Wen-bin PENG Ming key laboratory of biology and Genetic Resources of tropical crops Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101P.R.China key laboratory for biology and Genetic Resources of tropical crops of hainan Province Hainan Institute for Tropical Agricultural ResourcesHaikou 571101P.R.China Cash crops research institute Guangxi Academy of Agricultural SciencesNanning 530007P.R.China College of tropical crops Hainan UniversityHaikou 570228P.R.China

Cassava is an important tropical cash crop. Severe drought stresses affect cassava productivity and quality, and cause great economic losses in agricultural production. Enhancing the drought tolerance of cassava can effectively improve its yield. Long non-coding RNAs(lncRNAs) are present in a wide variety of eukaryotes. Recently, increasing evidence has shown that lncRNAs play a critical role in the responses to abiotic stresses. However, the function of cassava lncRNAs in the drought response remains largely unknown. In this study, we identified a novel lncRNA, DROUGHT-INDUCED INTERGENIC lncRNA(DIR). Gene expression analysis showed that DIR was significantly induced by drought stress treatment, but did not respond to abscisic acid(ABA) or jasmonic acid(JA) treatments. In addition, overexpression of the DIR gene enhanced proline accumulation and drought tolerance in transgenic cassava. RNA-seq analysis revealed that DIR preferentially affected drought-related genes that were linked to transcription and metabolism. Moreover, RNA pull-down mass spectrometry analysis showed that DIR interacted with 325 proteins. A protein–protein interaction(PPI)analysis found a marked enrichment in proteins associated with the mRNA export and protein quality control ***, these results suggest that DIR and its interacting proteins that regulate mRNA or protein metabolism are involved in mediating the drought stress response. Thus, regulating DIR expression has potential for improving cassava yield under drought conditions.

关键词： lncRNA RNA-seq drought stress cassava DIR

Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling

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Horticultural Plant Journal 2024年第2期10卷 532-544页

作者： Muhammad Ahsan Altaf Yuanyuan Hao Huangying Shu Weiheng Jin Chuhao Chen Lin Li Yu Zhang Muhammad Ali Mumtaz Huizhen Fu Shanhan Cheng Guopeng Zhu Zhiwei Wang key laboratory for Quality Regulation of tropical Horticultural crops of hainan Province Sanya Nanfan Research InstituteHainan UniversitySanyaHainan 572025China key laboratory for Quality Regulation of tropical Horticultural crops of hainan Province School of HorticultureHainan UniversityHaikouHainan 570228China hainan Yazhou Bay Seed laboratory SanyaHainan 572025China

This study assessed the influence of exogenous ME in the mitigation of cold damage in pepper seedlings. Melatonin(ME) is a dynamic molecule that helps plants cope with stress in several ways. Cold stress(CS) is one of the most important environmental factors that restrict plant growth and yield. Pepper(Capsicum annuum L.) is a valuable commercial crop, highly sensitive to CS. Thus, identifying an efficient strategy to mitigate cold damage is critical for long-term pepper production. For this purpose, the roots of pepper seedlings were pretreated with ME(5 μmol · L^(-1)) and exposed to CS for 7 d. The results indicated that CS suppressed pepper growth, hampered photosynthetic capacity, and damaged root architecture in pepper plants. In contrast, the production of reactive oxygen species(ROS), malondialdehyde(MDA), electrolyte leakage(EL), proline, and soluble sugars were enhanced in plants under CS. ME(5 μmol · L^(-1)) pretreatment reduced the negative effects of CS by recovering plant growth, root traits, gas exchange elements, and pigment molecules compared to CS control treatment. Furthermore, ME application efficiently reduced oxidative stress markers [hydrogen peroxide(H_(2)O_(2)), superoxide ion(O_(2)^(·-)), EL, and MDA] while increasing proline and soluble sugar content in pepper leaves. ME application combined with CS further increased antioxidant enzymes and related gene expression. Collectively, our results confirmed the mitigating potential of ME supplementation for CS by maintaining pepper seedling growth,improving the photosynthesis apparatus, regulating pigments, and osmolyte content.

关键词： Pepper Melatonin Cold stress Antioxidant enzyme Root trait

Association Analysis for Quality Traits in a Diverse Panel of Chinese Sesame(Sesamum indicum L.)Germplasm

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Journal of Integrative Plant biology 2013年第8期55卷 745-758页

作者： Wenliang Wei Yanxin Zhang Haixia Lü Donghua Li Linhai Wang Xiurong Zhang oil crops research institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture

The main objective of this study was to evaluate the potential of a sesame （Sesamum indicum L.） panel for association analysis, and investigate the genetic basis of oil content （OC）, protein content, oleic acid concentration, and linoleic acid concentration using association mapping. A panel of 216 sesame accessions was phenotyped in a multi-environment trial and fingerprinted with 608 polymorphic loci produced by 79 primers, including simple sequence repeats （SSRs）, sequence-related amplified polymorphisms （SRAPs）, and amplified fragment length polymorphisms （AFLPs）. Population structure analysis revealed two subgroups in the population. The Q model performed better for its ability to re-identify associations for the four traits at highly significant P-values compared to the other three mixed models. And a total of 35 and 25 associations for the four traits in 2010 and 2011 were identified, respectively, with the Q model after Bonferroni correction. Among those associations, only one for OC was re-identified in two environments, and several markers associated simultaneously with multiple traits were discovered. These results suggest the power and stability of the Q model for association analysis of nutritional traits in this sesame panel for its slight population stratification and familial relationship, which could aid in dissecting complex traits, and could help to develop strategies for improving nutritional quality.

关键词： Association mapping nutritional trait population structure Sesamum indicum L.

Molecular evidence for blocking erucic acid synthesis in rapeseed(Brassica napus L.) by a two-base-pair deletion in FAE1(fatty acid elongase 1)

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Journal of Integrative Agriculture 2015年第7期14卷 1251-1260页

作者： WU Lei JIA Yan-li WU Gang LU Chang-ming oil crops research institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture

DNA sequences of fatty acid elongase 1 genes FAE1.1 （EA） and FAE1.2 （Ec） were isolated and characterized for 30 com- mercialized low erucic acid rapeseed （LEAR） cultivars in China. Four types of independent mutation leading to low erucic acid trait were found, i.e., a single-base transition （eAl）, a two-base deletion （ec2） and four-base deletion （eC4） as well as single-base transition with a four-base deletion （eA.）. Three genotypes, i.e., eA1eA1eC2eC2, eA1eA1eC4eC4 and ***4ec4 were responsible for LEA content in storage Iipids of different rapeseed cultivars. Most of the LEAR cultivars had a genotype of eA1eA1ec2ec2, which were descended from the first LEAR cultivar, Oro. Yeast expression analysis revealed that two-base-pair （AA） deletion （ec2） at the base sites of 1 422-1 423 in the C genome FAE1 gene resulted in the absence of the condensing enzyme and led to the failure to produce erucic acid. Coexpression of FAE1 and ketoacyI-CoA reductase （KCR） or enoyI-CoA reductase （ECR） was found in high erucic acid rapeseed （HEAR） but not in LEAR （eA1eA1ec2ec2oreA1eA1ec4ec4）. Moreover, KCR and ECR were still coordinately regulated in eA1eA1ec2ec2 or eA1eA1ec4ec4 genotypes, suggesting that the expression of two genes was tightly linked. In addition, specific detection methods were developed by high-resolution melting curve analysis in order to detect eA1 and ec4.

关键词： erucic acid fatty acid elongase 1 natural mutation Brassica napus L.

Comparison of transcriptomes undergoing waterlogging at the seedling stage between tolerant and sensitive varieties of Brassica napus L.

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Journal of Integrative Agriculture 2015年第9期14卷 1723-1734页

作者： ZOU Xi-ling ZENG Liu LU Guang-yuan CHENG Yong XU Jin-song ZHANG Xue-kun key laboratory of biology and Genetic Improvement of oil crops Ministry of Agriculture/Oil Crops Research InstituteChinese Academy of Agricultural Sciences

RNA sequencing of the sensitive GH01 variety of Brassica napus L. seedling roots under 12 h of waterlogging was compared with previously published data of the ZS9 tolerant variety to unravel genetic mechanisms of waterlogging tolerance beyond natural variation. A total of 2 977 genes with similar expression patterns and 17 genes with opposite expression patterns were identiifed in the transcription proifles of ZS9 and GH01. An additional 1 438 genes in ZS9 and 1 861 genes in GH01 showed strain speciifc regulation. Analysis of the overlapped genes between ZS9 and GH01 revealed that waterlogging tolerance is determined by ability to regulate genes with similar expression patterns. Moreover, differences in both gene expression proifles and abscisic acid (ABA) contents between the two varieties suggest that ABA may play some role in waterlogging tolerance. This study identiifes a subset of candidate genes for further functional analysis.

关键词： rapeseed(Brassica napus L.) waterlogging digital gene expression(DGE) roots transcriptome comparative analysis

Genetic dissection of root morphological traits as related to potassium use efficiency in rapeseed under two contrasting potassium levels by hydroponics

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Science China(Life Sciences) 2019年第6期62卷 746-757页

作者： Xiaoling Dun Jiaqin Shi Huiping Liu Jie Wang Xinfa Wang Hanzhong Wang oil crops research institute of the Chinese Academy of Agricultural Sciences/key laboratory of biology and Genetic Improvement of oil crops Ministry of Agriculture

To reveal the genetic basis of potassium use efficiency(KUE) in rapeseed, root morphology(RM), biomass and KUE-related traits were measured in a recombinant inbred line population with 175 F7 lines that were subjected to high-potassium(HK) and low-potassium(LK) treatments by hydroponics. A total of 109 significant QTLs were identified to be associated with the examined traits. Sixty-one of these QTLs were integrated into nine stable QTLs. The higher heritability for RM and biomass traits and lower heritability for KUE-related traits, as well as nine stable QTLs for RM traits and only two for KUE-related traits,suggested that regulating RM traits would be more effective than selecting KUE traits directly to improve KUE by markerassisted selection. Furthermore, the integration of stable QTLs identified in the HK, LK, high-nitrogen(HN) and low-nitrogen(LN) conditions gave 10 QTL clusters. Seven of these clusters were classified into major QTLs that explained 7.4%–23.7% of the total phenotypic variation. Five of the major QTL clusters were detected under all of the treated conditions, and four clusters were specifically detected under the LK and LN conditions. These common and specific QTL clusters may be useful for the simultaneous improvement of multiple traits by marker-assisted selection.

关键词： Brassica napus L. root traits potassium efficiency QTL mapping QTL cluster