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Effects of thidiazuron on the quality and storage properties of mango fruit during postharvest

FOOD QUALITY AND SAFETY 2024年第8期8卷 545-555页

作者： Jian, Tangxin Luo, Cong Wan, Wen Liang, Rongzhen Lu, Tingting Li, Yuze Xie, Fangfang Chen, Canbin Li, Xi Xie, Xiaona He, Xinhua guangxi Univ State Key Lab Conservat & Utilizat Subtrop Agrobi Guangxi Key Lab Agroenvironm & Agroprod Safety Natl Demonstrat Ctr Expt Plant Sci EducColl Agr Nanning Peoples R China Baise Univ Guangxi Key Lab Biol Mango Baise Peoples R China

Thidiazuron (TDZ) is used for the expansion of fruits, but excessive levels of TDZ lead to a decline in fruit quality. The appropriate concentration of TDZ for mango expansion without a decline in quality is not clear. In the present study, four different concentrations (5, 10, 15, and 20 mg/L) of TDZ were applied via spraying on mango plants, and several physiological and biochemical indicators were measured. The results showed that TDZ treatment significantly increased mango fruit size and single-fruit weight. In mango fruit, TDZ treatment decreased the disease index, delayed the increase in the malondialdehyde and H2O2 content, and maintained firmness and antioxidant capacities at a relatively high level during postharvest storage. At the same time, TDZ treatment delayed the decrease in the giberellin, indoleacetic acid and jasmonic acid content in mango, and reduced the accumulation of abscisic acid and ethylene. These trends are consistent with TDZ treatment leading to extension of the shelf life of mango. Furthermore, ethylene biosynthesis, signal transduction, and cell wall dismantling-related genes were investigated. The results indicated that the expression of the MiACS, MiETR2, MiERF113, MiERF010, MiERF054, MiEXP, MiPG14, MiPG21, MiCEL, and MiPEL genes in mango was inhibited under TDZ treatment compared with the control. In summary, TDZ treatment can significantly increase the size and weight of mango fruit and can extend its shelf life. The most suitable concentration is 10-15 mg/L TDZ, which will not affect the quality of mango fruit.

关键词： Mango thidiazuron quality storage properties postharvest

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Mechanism of modified Chaihu Shugan formula in the treatment of epilepsy based on IncRNA-UCA1/miR-187/MAPK8 pathway

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China Medical Abstracts(Internal Medicine) 2024年第1期41卷 13-14页

作者：李瑞暖 guangxi molec biol key lab TCM Prev&TreatGraduate SchGuangxi TCM UnivNanning 530023

Objective To observe the effects of Modified Chaihu Shugan Formula (MCSF) on the behavior of epileptic mice and their regulatory effects on long noncoding RNAUCA1(lncRNA-UCA1),microRNA-187(miR-187) and recombinant mitogen activated protein kinase 8(MAPK8).Methods Totally 80 mice were randomly divided into normal group,model group (pilocarpine hydrochloride 180 mg/kg).

关键词： epilepsy Chaihu UCA1

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THE　STUDY　OF　STRUCTURE　AND　DYNAMICS　OF　DINUCLEAR　COMPLEXES　［Mo（VI）］_2－EDTA　AND　［Mo（VI）］_2－EGTABY^1HNMR

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Chinese Chemical Letters 1996年第6期7卷 567-568页

作者： Rui Fang SONG Fei LI Guang Min LIU You Gang MAO Fei DU(key laboratory for molecular Spectra & Structure. Institute ofTheoretical Chemistry, Jinn University, Changchun. 130023) key lab. molec. Spectra/Structure Institute of Theoretical Chemistry Jilin University Changchun 130023

The 1H and 13C NMR spectra of the dinuclear complexes of molybdenum(VI) withethylenediaminetetraacetic acid ([Mo(VI)]2-EDTA) and 3,12-bis(carboxymethyl)-6.9-dioxa-3,12-diazatetra-decanedioic acid(tMo(VI)]2-EGTA) at various temperatures were measured. The solutionstructure of the two dinuclear complexes was determined and the possible exchange process of theisomers is suggested

关键词： EDTA STRUCTURE STUDY THE DYNAMICS OF COMPLEXES DINUCLEAR 络合试剂 EGTABY~1HNMR AND Mo VI

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Studies on HEPES Stabilizing M Intermediate of Bacteriorhodopsin

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Chemical Research in Chinese Universities 1995年第3期11卷 215-218页

作者： Fu, Xue-qi Li, Zheng-qiang Wang, Jin-dui Wang, Bao-hui Li, Bo-fu Li, Tie-jin JILIN UNIV NATL KEY LAB ENZYME ENGNCHANGCHUN 130023PEOPLES R CHINA JILIN UNIV KEY LAB MOLEC SPECTRUM & MOLEC STRUCTCHANGCHUN 130023PEOPLES R CHINA JILIN UNIV DEPT CHEMCHANGCHUN 130023PEOPLES R CHINA

EPES has proved to stabilize M intermediate of Bacteriorhodopsin. Absorp-tion and Raman spectra showed that there was a more stable form of M intermedi-ate in BR-HEPES solution. It was found by SPS that there were two... 详细信息

关键词： Bacteriorhodopsin M intermediate HEPES

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Scavenging Effect of Perylenequinone Hydroxyls on Free Radicals: A Theoretical Study

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Chinese Chemical Letters 1998年第12期9卷 0-0+0-0页

作者： Hong Yu ZHANG (Department of biology, Provincial key laboratory of Animal Resistancebiology, Shandong Teachers’ University, Jinan 250014) Shandong Teachers Univ Dept Biol Provincial Key Lab Anim Resistance Biol Jinan 250014 Peoples R China

The sendempirical method AM1 was used to calculate AHOF values ofperylenequinone (PQ), and HOF was used as a theoretical parameter to predict the scavengingability of Po hydroxyls. Owing to strong intramolecular hydrogen bonds and the electronattracting property of the keto-function, hydroxyls of PQ were so weak in scavenging free radicalsthat the its effect was negligible in photodynandc therapy. However, when Po was substitilted withstrong electron-donating groups, its scavenging activity was enhanced markedly.

关键词： Perylenequinonoid derivatives antioxidant AM1 method △HOF value

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Role of plasminogen activator and plasminogen activator inhibitor type-1 in luteolysis——a minireview

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Chinese Science Bulletin 1997年第23期42卷 1994-2000页

作者： Liu, YX Chen, YJ Feng, QA Hu, ZY Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

This minireview summarized our recent studies on the role of plasminogen activator (PA) and inhibitor type-1 (PAI-1) in luteolysis. We have demonstrated that (1) both tissue type and urokinase type plasminogen activators (tPA and uPA) and a plasminogen activator inhibitor type-1 (PAI-1) were present in the corpus luteum of rat and rhesus monkey; (2) decrease in progesterone production in corpus luteum was well correlated with a sharp increase in tPA (but not uPA) and PAI-1 secretion; (3) exogenous tPA decreased luteal progesterone synthesis while monoclonal antibodies increased progesterone production; (4) interferon y inhibited luteal progesterone synthesis and stimulated tPA production while LH plus pro-lactin increased progesterone production and decreased tPA (but not uPA) activity in cultured luteal cells; (5) increase in proteolysis in the corpus luteum was also correlated with decrease in progesterone production in mouse. These data suggest that local degradation of extracellular matrix

关键词： tissue type plasminogen activator (tPA) plasminogen activator inhibitor type-1 (PAI-1) Iuteolysis.

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Expression and regulation of plasminogen activator and plasminogen activator inhibitor type-1 in rat epididymis

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Chinese Science Bulletin 1997年第9期42卷 779-783页

作者： Zhou, HM Zhang, T Liu, YX CHINESE ACAD SCI INST ZOOLSTATE KEY LAB REPROD BIOLBEIJING 100080PEOPLES R CHINA

EPIDIDYMIS is the site where spermatozoa released from testis undergo maturation and become capable of fertilizing *** epididymal secretions including enzymes,nutrients and hor-mones are believed to be essential for sperm *** proteolysis contributes toloss or modification of surface molecules of spermatozoa,which is an important aspect of

关键词： plasminogen activator plasminogen activator inhibitor human chorionic gonadotropin/luteinizing hormone epi-didymis.

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Localization and expression of TR3 orphan receptor protein and its mRNA in rat

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Chinese Science Bulletin 1998年第2期43卷 146-149页

作者： Xiaomin Mu Yixun Liu Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

The cellular localization and specific expression of TR3 mRNA in rat testis were investigated by the method of immunohistochemistry and in situ hybridization. It was demonstrated that orphan receptor TR3 was expressed in a significant amount in rat testis and TR3 protein and mRNA were specifically localized in germ cells, suggesting that TR3 may have an important function in regulating rat germ cell development.

关键词： orphan receptor TR3 immunohistochemistry in situ hybridization spermatogenesis.

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Site-specific uv crosslinking of minihelix DNA and TrpRS from Bacillus subtilis

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Chinese Science Bulletin 2001年第16期46卷 1380-1383页

作者： Xu, F Jiang, G Zhang, Y Zhu, LF Jin, YX Wang, DB Chinese Acad Sci Shanghai Inst Biol Sci Inst Biochem & Cell Biol State Key Lab Mol Biol Shanghai 200031 Peoples R China

In order to investigate the recognition mechanism and the relationship between structure and function of minihelix DNA with Tryptophanyl-tRNA Synthetase (TrpRS), TrpRS from Bacillus Subtilis was purified. Four minihelix DNAs were chemically synthesized and the photoreactive reagent s4T was incorporated into three of them at the positions of G73, T72 and T55 corresponding to tRNATrp. The apparatus for uv crosslinking was devised and the parameters for uv crosslinking were optimized. The results indicated that the G73 and T72 base of minihelix DNA interacted with TrpRS directly. The uv crosslinking reaction was improved by the dose of uv irradiation and the concentration of both TrpRS and minihelix DNA.

关键词： uv crosslinking minihelix DNA TrpRS discriminator.

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Non-radioisotopic method for the in vitro measurement of EGF receptor tyrosine kinase

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Chinese Science Bulletin 2001年第8期46卷 683-685页

作者： Gaoxiang Ge Jing Wu Qishui Lin Chinese Acad Sci Shanghai Inst Biol Sci Inst Biochem & Cell Biol State Key Lab Mol Biol Shanghai 200031 Peoples R China

A non-radioisotopic method was developed for the assay of epidermal growth factor receptor (EGFR). A peptide with twenty amino acid residues around Tyr 1173, the major phosphorylation site of EGFR, was cloned as a GST fusion protein and used as substrate. Anti-phosphoty-rosine monoclonal antibody PY99 was used for the determination of the extent of phosphorylation. Both the specificity and the sensitivity were substantially higher than that of the existing method. Km value of the fusion protein is much lower (10 μmol/L) than that of the synthetic peptide (110 μmol/L). The method can be applied to the measurement of the tyrosine kinase activity of c-erb B2 (Neu/HER2).

关键词： EGF receptor tyrosine kinase nonradioisotopic GST in vitro.

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