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Dietary calcium supplementation promotes the accumulation of intramuscular fat

Journal of Animal Science and Biotechnology 2022年第2期13卷 536-550页

作者： Zhiwang Zhang Tingli Pan Yu Sun Siqi Liu Ziyi Song Haojie Zhang Yixing Li Lei Zhou State key laboratory for conservation and utilization of Subtropical Agro-bioresources College of Animal Science and TechnologyGuangxi UniversityGuangxi Zhuang Autonomous RegionNanning 530004People’s Republic of China

Background:In the livestock industry,intramuscular fat content is a key factor affecting meat *** studies have shown that dietary calcium supplementation is closely related to lipid ***,few studies have examined the relationship between dietary calcium supplementation and intramuscular fat ***:Here,we used C2C12 cells,C57BL/6 mice(n=8)and three-way cross-breeding pigs(Duroc×Landrace×Large white)(n=10)to study the effect of calcium addition on intramuscular fat *** vitro,we used calcium chloride to adjust the calcium levels in the medium(2 mmol/L or 3 mmol/L).Then we measured various *** vivo,calcium carbonate was used to regulate calcium levels in feeds(Mice:0.5%calcium or 1.2%calcium)(Pigs:0.9%calcium or 1.5%calcium).Then we tested the mice gastrocnemius muscle triglyceride content,pig longissimus dorsi muscle meat quality and ***:In vitro,calcium addition(3 mmol/L)had no significant effect on cell proliferation,but promoted the differentiation of C2C12 cells into slow-twitch *** supplementation increased triglyceride accumulation in C2C12 *** addition increased the number of mitochondria and also increased the calcium level in the mitochondria and reduced the of key enzymes activity involved inβ-oxidation such as acyl-coenzyme A *** mitochondrial calcium level can alleviate lipid accumulation induced by calcium *** addition,calcium addition also reduced the glycolytic capacity and glycolytic conversion rate of C2C12 *** vivo,dietary calcium supplementation(1.2%)promoted the accumulation of triglycerides in the gastrocnemius muscle of *** calcium supplementation(1.5%)had no effect on pig weight,but significantly improved the flesh color of the longissimus dorsi muscle,reduced the backfat thickness and increased intramuscular fat content in ***,calcium addition had no effect on longissimus dorsi pH,electrical conducti

关键词： C2C12 Calcium Intramuscular fat Meat quality Pig

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A rhizobia strain isolated from root nodule of gymno-sperm Podocarpus macrophyllus

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Science China(Life Sciences) 2007年第2期50卷 228-233页

作者： HUANG BaoLing, LU ChengQun, WU Bo & FAN LiQin 1 Forestry College, guangxi University, Nanning 530004, China 2 guangxi key laboratory of Subtropical Bioresource conservation and utilization, Nanning 530004, China 1. Forestry College Guangxi University Nanning 530004 China 2. guangxi key laboratory of Subtropical Bioresource conservation and utilization Nanning 530004 China

Symbiotic nitrogen fixation of rhizobia and leguminous plants is considered as the most important biologic nitrogen fixation system on earth. Symbiotic nodulation of gymnosperm Podocarpus macro-phyllus and rhizobia has never been reported. In this study, 11 endophytic bacteria strains were isolated from root nodules of P. macrophyllus and its variation P. macrophyllus var. maki. The plant infection tests on these strains indicated that the isolated strains could be nodulated on P. macrophyllus plants, and weak nitrogenase activity of nodules was found in acetylene reduction method. According to the physiological and biochemical characteristics of the 11 strains, GXLO 02 was selected as the representative strain. 16S rDNA full-length sequence analysis of GXLO 02 confirmed that the representative strain GXLO 02 belongs to Rhizobium sp.

关键词： Podocarpus macrophyllus P. macrophyllus var. maki rhizobia physiological and biochemical characteristics 16S rDNA sequence

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Characterization of the Promoter of a Homolog of Maize MADS-Box Gene m18

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Journal of Integrative Agriculture 2014年第11期13卷 2330-2345页

作者： QIN Hui-juan PAN Hong FAN Xian-wei WU Qiao LI You-zhi key laboratory for Microbial and Plant Genetic Engineering Ministry of Education/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Ministry of Science and Technology/College of Life Science and Technology Guangxi University

Maize （Zea mays L.） is one of the world’s major food crops, and often suffers from tremendous yield loss caused by abiotic stresses. The MADS-box genes are known to play versatile roles in plants, controlling plant responses to multiple abiotic stresses. However, understanding of regulation of their expressions by the conventional loss-of-function approach is very dififcult. So far, regulation of MADS-box gene expression is little known. The best approach to retrieve expression regulation of this category of genes is to characterize expression of their promoters. In this study, the promoter of a homolog （GenBank accession no. EC864166） of maize MADS-box gene m18 was cloned by way of genome-walking PCR, named Pro66. Predicative analysis indicated that Pro66 contains more than one TATA box and multiple cis-acting environmental conditions-responsive elements （ECREs）. Pro66 could drive expression of theβ-glucuronidase （GUS）-encoding gene in maize, and heterologous expression of GUS in red pepper stressed by water deifcit, salt, copper, iron deifciency, heat, cold, and grown under short and long photoperiods, echoing predicative ECREs. Conclusively, maize MADS-box gene m18 likely plays versatile functions in maize response to multiple abiotic stresses due to the promoter with multiple cis-acting elements. The complex arrangement of multiple cis-acting elements in the promoter features meticulously regulated expression of m18. The results give informative clues for heterologous utilisation of the promoters in monocot and dicot species. The copy of the ECREs and heterologous expression of the promoter in dicot species are also discussed.

关键词： maize MADS-box gene promoter stress transcription

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Identification of Major Locus Bph35 Resistance to Brown Planthopper in Rice

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Rice science 2020年第3期27卷 237-245页

作者： ZHANG Yuexiong QIN Gang MA Qianqian WEI Minyi YANG Xinghai MA Zengfeng LIANG Haifu LIU Chi LI Zhenjing LIU Fang HUANG Dahui LI Rongbai College of Agriculture and State key laboratory for conservation and utilization of Subtropical Agro-Bioresources Guangxi UniversityNanning 530007China Rice Research Institute Guangxi Academy of Agricultural SciencesNanning 530007China

An introgression line RBPH660,derived from wild rice Oryza rufipogon,showed stable resistance to brown planthopper(BPH).Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/*** using the bulked segregant analysis(BSA)-seq method,two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660,respectively.A major resistance locus,designated as Bph35 accounting for 51.27%of the phenotypic variation with a LOD score of 42.51,was mapped to the candidate region of chromosome 4 between In Del(insertion-deletion)markers PSM16 and R4 *** fine mapping of Bph35,one simple sequence repeat and three newly developed In Del markers were used to screen the ***,the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism(SNP)variant sites between the resistant and susceptible *** of these genes,Os04 g0193950,encoding a putative NB-ARC(nucleotidebinding adaptor shared by APAF-1,R proteins and CED-4)and LRR(leucine-rich repeat)domain protein with nine non-synonymous SNP substitutions in its coding sequence regions,might be the candidate gene for *** findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice.

关键词： rice brown planthopper Bph35 bulked segregant analysis(BSA)-seq method gene mapping

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The autophagy gene ATG8 affects morphogenesis and oxidative stress tolerance in Sporisorium scitamineum

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Journal of Integrative Agriculture 2019年第5期18卷 1024-1034页

作者： ZHANG Bin CUI Guo-bing CHANG Chang-qing WANG Yi-xu ZHANG Hao-yang CHEN Bao-shan DENG Yi-zhen JIANG Zi-de Department of Plant Pathology/State key laboratory for conservation and utilization of Subtropical Agro-Bioresources South China Agricultural University Integrative Microbiology Research Centre/Guangdong Province key laboratory of Microbial Signals and Disease Control South China Agricultural University College of Life Science and Technology/State key laboratory for conservation and utilization of Subtropical Agro-Bioresources Guangxi University

The basidiomycetous fungus Sporisorium scitamineum causes sugarcane smut that leads to severe economic losses in the major sugarcane growing areas in China,India and Brazil,*** is a conserved pathway in eukaryotes for bulk degradation and cellular recycling,and was shown to be important for fungal cell growth,development,and ***,physiological function of autophagy has not been studied in *** this study,we identified a conserved Atg8 protein,named as SsAtg8 and characterized its *** results showed that autophagy was blocked in the ssatg8Δ mutant,in nitrogen *** ssatg8Δ mutant formed pseudohypha frequently and was hypersensitive to oxidative ***,mating or filamenation was unaffected in the ssatg8Δ mutant in *** we demonstrate that autophagy is dispensable for *** mating/filamentation,while critical for oxidative stress tolerance and proper morphology in sporidial stage.

关键词： Atg8 autophagy fungus Sporisorium scitamineum morphogenesis oxidative stress tolerance

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Matrix metalloproteinase 2 contributes to adult eclosion and immune response in the small hive beetle,Aethina tumida

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Insect Science 2024年第3期31卷 733-747页

作者： Lixian Wu Yajing Xu Liangbin Li Dainan Cao Fang Liu Hongxia Zhao Guangdong key Laboratssory of Animal conservation and Resource utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationInstitute of ZoologyGuangdong Academy of SciencesGuangzhouChina

During the pupal-adult eclosion process of holometabolous insects,the old cuticle is shed and replaced by a completely different new cuticle that requires tanning and expansion,along with extensive extracellular matrix(ECM)*** vertebrates,matrix metalloproteinases(MMPs),a class of zinc-dependent endopeptidases,play key roles in regulating the ECM that surrounds ***,little is known about these extracellular proteinases available in *** small hive beetle(SHB),Aethina tumida,is a widespread invasive parasite of honey *** this study,6 MMP homologs were identified in the SHB *** interference experiments showed that all 6 AtMmps are not required for the larval-pupal transition,only AtMmp2 was essential for pupal-adult eclosion in *** of AtMmp2 resulted in eclosion defects and wing expansion failure,as well as mortality within 3 d of adult *** analysis revealed that knockdown of AtMmp2 significantly increased expression of the Toll and Imd pathways,chitin metabolism,and cross-linking(such as the pro-phenoloxidase activating cascade pathway and the tyrosine-mediated cuticle sclerotization and pigmentation pathway).These data revealed evolutionarily conserved functions of Mmp2 in controlling adult eclosion and wing expansion,also provided a preliminary exploration of the novel function of regulating Toll and Imd pathways,as well as new insights into how MMPs regulate insect development and defense barriers.

关键词： Aethina tumida adult eclosion immune response matrix metalloproteinase RNAinterference

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Roles of fishing and climate change in long-term fish species succession and population dynamics in the outer Beibu Gulf,South China Sea

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Acta Oceanologica Sinica 2019年第10期38卷 1-8页

作者： Xuehui Wang Yongsong Qiu Feiyan Du Weida Liu Dianrong Sun Xiao Chen Weiwen Yuan Yong Chen South China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesGuangzhou 510300China key laboratory of Open-Sea Fishery Development Ministry of Agriculture and Rural AffairGuangzhou 510300China Guangdong Provincial key laboratory of Fishery Ecology and Environment Guangzhou 510300China College of Marine Sciences South China Agricultural UniversityGuangzhou 510642China School of Marine Sciences University of MaineOronoMaine 04469USA guangxi Academy of Sciences Guangxi Mangrove Research CenterGuangxi Key Lab of Mangrove Conservation and UtilizationBeihai 536000China

A prevailing,controversial hypothesis is that fishing pressure has played a greater role than climatic and environmental drivers,in changing fish species succession and biomass fluctuation in the South China Sea(SCS).Based on otter trawl survey data from 1959 to 2010 in the outer Beibu Gulf(OBG),northern SCS,large seasonal and interannual variation is reported for fish species composition,the proportional abundances of dominant taxa,and fish *** additive models are developed to quantify relationships between fish biomass and the external factors of fishing pressure and climate *** pressure proved to be the main driver of sharp declines in demersal fish stocks,with high-value species being replaced by low-value ones over *** decreases in fish biomass during the years of 1993 and 1998 correspond to El Nino events,with climate change possibly the main driver of proportional representation of pelagic species in fisheries trawl *** need to differentiate impacts of fishing and environmental drivers on fish species with different life history strategies is stressed to better understand fish community dynamics.

关键词： fish species succession biomass fluctuation fishing effect climate influence Beibu Gulf northernSouth China Sea

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Aboveground biomass stocks of species-rich natural forests in southern China are influenced by stand structural attributes,species richness and precipitation

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Plant Diversity 2024年第4期46卷 530-536页

作者： Wen-Hao Zeng Shi-Dan Zhu Ying-Hua Luo Wei Shi Yong-Qiang Wang Kun-Fang Cao State key laboratory for conservation and utilization of Subtropical Agro-Bioresources College of ForestryGuangxi UniversityNanning 530004China guangxi key laboratory of Forest Ecology and conservation College of ForestryGuangxi UniversityNanning 530004China key laboratory of National Forestry and Grassland Administration on Biodiversity conservation in Karst Mountainous Areas of Southwestern China School of Life SciencesGuizhou Normal UniversityHuaxi College TownGui'an DistrictGuiyang 550025China

Forests,the largest terrestrial carbon sinks,play an important role in carbon sequestration and climate change *** forest attributes and environmental factors have been shown to impact aboveground biomass,their influence on biomass stocks in species-rich forests in southern China,a biodiversity hotspot,has rarely been *** this study,we characterized the effects of environmental factors,forest structure,and species diversity on aboveground biomass stocks of 30 plots(1 ha each) in natural forests located within seven nature reserves distributed across subtropical and marginal tropical zones in guangxi,*** results indicate that forest aboveground biomass stocks in this region are lower than those in mature tropical and subtropical forests in other ***,we found that aboveground biomass was positively correlated with stand age,mean annual precipitation,elevation,structural attributes and species richness,although not with species *** we compared stands with the same basal area,we found that aboveground biomass stock was higher in communities with a higher coefficient of variation of diameter at breast *** findings highlight the importance of maintaining forest structural diversity and species richness to promote aboveground biomass accumulation and reveal the potential impacts of precipitation changes resulting from climate warming on the ecosystem services of subtropical and northern tropical forests in ***,many natural forests in southern China are not fully ***,their continued growth will increase their carbon storage over time.

关键词： Subtropical forest Marginal tropical forest Aboveground biomass Species diversity Forest structural attribute Environment factor

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Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection

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Journal of Animal Science and Biotechnology 2015年第4期6卷 395-401页

作者： Fanli Meng Hui Li Xiaoli Wang Guangsheng Qin Bj?rn Oback Deshun Shi Animal Reproduction Institute State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesGuangxi University AgResearch Ltd. Ruakura Research CentreReproductive Technologies

Background： Cytoplasmic injection of exogenous DNA into zygotes is a promising technique to generate transgenic livestock. However, it is still relatively inefficient and has not yet been demonstrated to work in buffalo. We sought to improve two key technical parameters of the procedure, namely i） how much linear DNA to inject and ii） when to inject it. For this, we introduced a constitutively expressed enhanced green fluorescent protein （EGFP） plasmid into buffalo zygotes. Results： First, we found that the proportion of EGFP-expressing blastocysts derived from zygotes injected with 20 or 50 ng/pL DNA was significantly higher than from those injected with 5 pg/mL. However, 50 ng/pL exogenous DNA compromised blastocyst development compared to non-injected IVF controls. Therefore the highest net yield of EGFP-positive blastocysts was achieved at 20 ng/pL DNA. Second, zygotes injected early （7-8 h post-insemination [hpi]） developed better than those injected at mid （12-13 hpi） or late （18-19 hpi） time points. Blastocysts derived from early injections were also more frequently EGFP-positive. As a consequence, the net yield of EGFP-expressing blastocysts was more than doubled using early vs late injections （16.4 % vs 7.7 %）. With respect to blastocyst quality, we found no significant difference in cell numbers of EGFP-positive blastocysts vs non-injected blastocysts. Following embryo transfer of six EGFP-positive blastocysts into four recipient animals, two viable buffalo calves were born. Biopsied ear tissues from both buffalo calves were analyzed for transgene presence and expression by Southern blot, PCR and confocal laser scanning microscopy, respectively. This confirmed that both calves were transgenic. Conclusions： Our cytoplasmic injection protocol improved generation of transgenic embryos and resulted in the first transgenic buffalo calves produced by this method.

关键词： Buffalo Cytoplasmic injection Plasmid Transgenic Zygote

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2-D Numerical Simulation of Natural Gas Hydrate Decomposition Through Depressurization by Fully Implicit Method

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China Ocean Engineering 2009年第3期23卷 529-542页

作者：宋永臣梁海峰 key laboratory of Ocean Energy utilization and Energy conservation of Ministry of Education Dalian University of Technology

Natural gas hydrate, as a potential energy resource, deposits in permafrost and marine sediment with large quantities. The current exploitation methods include depressurization, thermal stimulation, and inhibitor injection. However, many issues have to be resolved before the commercial production. In the present study, a 2-D axisymmetric simulator for gas production from hydrate reservoirs is developed. The simulator includes equations of conductive and convective heat transfer, kinetic of hydrate decomposition, and multiphase flow. These equations are discretized based on the finite difference method and are solved with the fully implicit simultaneous solution method. The process of laboratory-scale hydrate decomposition by depressurization is simulated. For different surrounding temperatures and outlet pressures, time evolutions of gas and water generations during hydrate dissociation are evaluated, and variations of temperature, pressure, and multiphase fluid flow conditions are analyzed. The results suggest that the rate of heat transfer plays an important role in the process. Furthermore, high surrounding temperature and low outlet valve pressure may increase the rate of hydrate dissociation with insignificant impact on final cumulative gas volume.

关键词： natural gas hydrate depressurization fully implicit simuhaneoas solution method impact parameters analysis

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