Matrix metalloproteinase 2 contributes to adult eclosion and immune response in the small hive beetle,Aethina tumida

作     者：Lixian Wu Yajing Xu Liangbin Li Dainan Cao Fang Liu Hongxia Zhao 

作者机构：Guangdong Key Laboratssory of Animal Conservation and Resource UtilizationGuangdong Public Laboratory of Wild Animal Conservation and UtilizationInstitute of ZoologyGuangdong Academy of SciencesGuangzhouChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2024年第31卷第3期

页      面：733-747页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：funded by GDAS Special Project of Science and Technology Development(2022GDASZH-2022010106) National Natural Science Foundation of China(Grant No.32200405),Guangzhou Basic and Applied Basic Research Foundation(202201010255) Guangdong Basic and Applied Basic Research Foundation(2021A1515012102,2021A1515010630) Science and Technology Planning Project of Yunfu City(2022020425) 

主　　题：Aethina tumida adult eclosion immune response matrix metalloproteinase RNAinterference 

摘      要：During the pupal-adult eclosion process of holometabolous insects,the old cuticle is shed and replaced by a completely different new cuticle that requires tanning and expansion,along with extensive extracellular matrix(ECM)*** vertebrates,matrix metalloproteinases(MMPs),a class of zinc-dependent endopeptidases,play key roles in regulating the ECM that surrounds ***,little is known about these extracellular proteinases available in *** small hive beetle(SHB),Aethina tumida,is a widespread invasive parasite of honey *** this study,6 MMP homologs were identified in the SHB *** interference experiments showed that all 6 AtMmps are not required for the larval-pupal transition,only AtMmp2 was essential for pupal-adult eclosion in *** of AtMmp2 resulted in eclosion defects and wing expansion failure,as well as mortality within 3 d of adult *** analysis revealed that knockdown of AtMmp2 significantly increased expression of the Toll and Imd pathways,chitin metabolism,and cross-linking(such as the pro-phenoloxidase activating cascade pathway and the tyrosine-mediated cuticle sclerotization and pigmentation pathway).These data revealed evolutionarily conserved functions of Mmp2 in controlling adult eclosion and wing expansion,also provided a preliminary exploration of the novel function of regulating Toll and Imd pathways,as well as new insights into how MMPs regulate insect development and defense barriers.