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Long duplication of 18S ribosomal DNA in Cynoglossus lineolatus(Pleuronectiformes： Cynoglossidae)： novel molecular evidencefor unequal crossing over model

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Acta Oceanologica Sinica 2016年第12期35卷 38-50页

作者： GONG Li SHI Wei YANG Min SI Lizhen KONG Xiaoyu guangxi key laboratory of mangrove conservation and utilization Guangxi Mangrove Research Center Guangxi Academy of Sciences Beihai 536000 China key laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Provincial key laboratory of Applied Marine Biology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510000 China University of the Chinese Academy of Sciences Beijing 100049 China

Although 18 S r DNA sequence is extremely conservative, the polymorphism still has been found in few *** the present study, three types(Type A, B and C) of 18 S r DNA sequence coexisted in Cynoglossus lineolatus genome, suggesting a non-concerted evolution process, rather than a strictly concerted evolution *** on the differences of sequence variation, GC content, secondary structure and minimum free energy,Types A and B were speculated as the potential pseudogenes. Additionally, a fascinating finding was a 189-bp duplication of 18 S r DNA in Type A sequence. To our knowledge, this is the first report on such a long duplication in teleostean ribosomal DNA. Compared with several theories accounting for the formation of tandem repeats, the unequal crossing over model was thought to be the most likely mechanism to generate the189-bp duplication of 18 S r DNA. These results not only provide a novel molecular evidence for the unequal crossing over model, but also benefit for the further study on 18 S r DNA in fishes.

关键词： nrDNA Cynoglossus lineolatus tandem repeat pseudogene polymorphism non-concerted evolution

Construction and characterization of a hrpG mutant rendering constitutive expression of hrp genes in Xanthomonas campestris ***

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Progress in Natural Science:Materials International 2006年第5期16卷 480-485页

作者： JIANG Bole, XU Rongqi, LI Xianzhen, WEI Hongyu, BAI Faan, HU Xi, HE Yongqiang and TANG Jiliang ( The guangxi key laboratory of Subtropical Bioresources conservation and utilization,College of Life Science and Technology of guangxi University, Nanning 530004, China) [a]The guangxi key laboratory of Subtropical Bioresources conservation and utilization [b]College of Life Science and Technology of guangxi University Nanning China

The expression of hrp genes in Xanthomonas is regulated by hrpG, an OmpR-type transcriptional activator, and can be induced by artificial mimic medium XVM2, but repressed in rich medium NYG. In this study, a site-specific mutation of hrpG in Xanthomonas campestris pv. campestris (Xcc) was generated by PCR-directed point mutagenesis and was characterized by its phenotype and transcriptional expression analyses. The results indicated that the E45K mutated hrpG rendered all the hrp genes expressing constitutively in the suppression medium NYG. Plant test showed that mutated hrpG enhanced the timing and intensity of hypersensitive reaction (HR) on the nonhost plant pepper, but did not affect the growth of Xcc in both culture medium and plants. The E45K mutation did not cause significant changes of production of exopolysaccharide, extracellular cellulase and amylase, but it inhibited the secretion of extracellular protease. This mutant 8004 is more suitable than the wild type for identifying hrp-dependent effectors in vitro and studying the transcriptional regulation genome-wide.

关键词： hrpG Xanthomonas campestris pv. campestris effector constitutive expression site-specific mutagenesis.

Interaction between Heparin and Fibronectin: Using Quartz Crystal Microbalance with Dissipation, Immunochemistry and Isothermal Titration Calorimetry

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Journal of Wuhan University of Technology(Materials Science) 2015年第5期30卷 1074-1084页

作者：李贵才 WANG Caiping 杨苹 ZHOU Jie ZHU Pingchuan Jiangsu key laboratory of Neuroregeneration Nantong University key laboratory for Advanced Technologies of Materials Ministry of Education Southwest Jiaotong University guangxi key laboratory for Subtropical Bio-Resource conservation and utilization Guangxi University

The adsorption behavior of heparin and fibronectin was studied by quartz crystal microbalance with dissipation(QCM-D), and the interaction between heparin and fibronectin was evaluated using immunochemistry and isothermal titration calorimetry(ITC) measurement. The results showed that there was competitive adsorption between heparin and fibronectin, and the preadsorption of fibronectin could prevent subsequent heparin adsorption to some extent, and the adsorbed Hep/Fn complex on the surface was in a rigid form. The bioactivity of heparin and fibronectin could be affected by the bulk concentration of each, and both heparin and fibronectin in Hep/Fn complex formed under p H 4 condition displayed larger bioactivity than that formed under p H 7 condition. Moreover, the fibronectin showed more exposed cell-binding sites at the p H value lower than physiological condition. The results of ITC further suggested that the interaction between heparin and fibronectin under p H 4 was stronger than under p H 7, and the complex was also more stable. The study brings forth the detailed interaction between heparin and fibronectin, which will be helpful for better understanding the interaction mechanism of the two biomolecules. The results may be potentially useful for the development of new generation of cardiovascular biomaterials.

关键词： Heparin fibronectin QCM-D immunochemistry isothermal titration calorimetry

Manganese toxicity-induced chlorosis in sugarcane seedlings involves inhibition of chlorophyll biosynthesis

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The Crop Journal 2022年第6期10卷 1674-1682页

作者： Shu Yang Guizhi Ling Qiuyue Li Ke Yi Xinlian Tang Muqing Zhang Xiaofeng Li State key laboratory for conservation and utilization of Subtropical Agro-bioresources/guangxi key laboratory for Sugarcane Biology College of AgricultureGuangxi UniversityNanning 530004GuangxiChina Institute for New Rural Development Guangxi UniversityNanning 530004GuangxiChina

Manganese(Mn)toxicity-induced leaf chlorosis limits crop production in acidic soils,but its underlying mechanisms remain *** effects of excessive Mn on chlorophyll(Chl)biosynthesis in sugarcane(Saccharum officinarum L.)leaves were *** Mn treatment,Chl concentration decreased with Mn accumulation and chlorosis appeared in expanding *** that,levels of the initial Chl precursor 5-aminolevulinic acid(ALA)and its downstream intermediates decreased,whereas magnesium-protoporphyrin IX monomethyl ester(MgPME)*** of Mn in leaves downregulated the ALA biosynthetic gene GluTR(encoding glutamyl-tRNA reductase)and MgPME conversion gene MgPMEC(encoding MgPME cyclase),upregulated the ALA biosynthesis inhibitor FLU(encoding FLUORESCENT),but had no significant effect on the expression of other Chl biosynthetic *** above Mn-induced changes of Chl precursors and expression of corresponding genes commenced before the Chl decline and leaf chlorosis,and were reversed by ALA ***,excessive Mn-induced chlorosis in sugarcane is mediated by a Chl-biosynthesis disorder resulting from the inhibition of ALA synthesis and MgPME conversion.

关键词： Sugarcane Manganese toxicity Chlorosis Chlorophyll biosynthesis Gene expression

Gas-Solid Flow,Combustion Characteristics,and Gas Emissions in a 75 t/h CFB Boiler Based on the CPFD Method

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Journal of Thermal Science 2025年第1期 323-336页

作者： HUANG Xiankun BAI Zhang ZHU Xiaoli WANG Shuoshuo MU Lin GONG Liang College of New Energy China University of Petroleum (East China) key laboratory of Ocean Energy utilization and Energy conservation of Ministry of Education School of Energy and Power EngineeringDalian University of Technology

A 3D simulation using Computational Particle Fluid Dynamics（CPFD） methods was used to calculate coal combustion in a 75 t/h industrial-scale circulating fluidized bed（CFB） *** characteristics,gas-solid flow characteristics,and gaseous pollutant emissions of CFB boilers from combustion ignition to stable operation were systematically evaluated in this *** show that the temperature distribution is relatively uniform throughout the *** the combustion process unfolds within the boiler,the gas composition curve strikingly portrays the inverse correlation between CO2and *** the combustion reaction progresses,it becomes evident that the concentration of CO2progressively increases,while the concentration of O2concurrently *** inverse relationship underscores the fundamental combustion reaction,where carbon-based fuels react with oxygen to produce carbon dioxide and release ***,a comprehensive analysis has revealed that,from ignition to stable combustion,both nitric oxide（NO） and sulfur dioxide（SO2） emissions exhibit a declining *** reduction in pollutant generation is attributed to the improvement in combustion *** complete combustion leads to lower levels of unburned hydrocarbons,which are prone to NO ***,the sulfur content in the fuel is more efficiently oxidized to sulfur trioxide（SO3） or bound in sulfates,reducing *** steady state in the simulation,the SO2mass flow rate varies significantly with the furnace height,gradually increasing from 0.07 kg·s-1at 4 m at the bottom of the furnace to a peak of 0.078 kg·s-1at 8 m in the center,and then decreasing to 0.06 kg·s-1at 20m at the top of the furnace.

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Structural and biochemical basis of FLS2-mediated signal activation and transduction in rice

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Plant Communications 2024年第3期5卷 216-231页

作者： Qiaoqiao Zhao Jinlin Bao Huailong Li Wei Hu Yanqiong Kong Yifeng Zhong Qiang Fu Guolyu Xu Fenmei Liu Xi Jiao Jian Jin Zhenhua Ming State key laboratory for conservation and utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi Key Laboratory for Sugarcane BiologyGuangxi UniversityNanning 530004P.R.China

The receptor-like kinase FLAGELLIN-SENSITIVE 2(FLS2)functions as a bacterialflagellin receptor local-ized on the cell membrane of *** Arabidopsis,the co-receptor BRI1-ASSOCIATED RECEPTOR KI-NASE 1(BAK1)cooperates with FLS2 to detect theflagellin epitopeflg22,resulting in formation of a signaling complex that triggers plant defense ***,the co-receptor responsible for recog-nizing and signaling theflg22 epitope in rice remains to be determined,and the precise structural mecha-nism underlying FLS2-mediated signal activation and transduction has not been *** study pre-sents the structural characterization of a kinase-dead mutant of the intracellular kinase domain of OsFLS2(OsFLS2-KDD1013A)in complex with ATP or ADP,resolved at resolutions of 1.98 A˚and 2.09 A˚,*** analysis revealed that OsFLS2 can adopt an active conformation in the absence of phosphorylation,although it exhibits only weak basal catalytic activity for ***-quent investigations demonstrated that OsSERK2 effectively phosphorylates OsFLS2,which reciprocally phosphorylates OsSERK2,leading to complete activation of OsSERK2 and rapid phosphorylation of the downstream substrate receptor-like cytoplasmic kinases OsRLCK176 and *** mass spectrometry experiments,we successfully identied critical autophosphorylation sites on OsSERK2,as well as sites transphosphorylated by ***,we demonstrated the interaction between OsSERK2 and OsFLS2,which is enhanced in the presence *** evidence suggests that OsRLCK176 and OsRLCK185 may function downstream of the OsFLS2-mediated signaling *** study reveals the molecular mechanism by which OsFLS2 mediates signal transduction pathways in rice and provides a valuable example for understanding RLK-mediated signaling pathways in plants.

关键词： FLS2 active conformation kinase activation transphosphorylation signal transduction

Regulation of eight avr genes by hrpG and hrpX in Xanthomonas campestris pv. campestris and their role in pathogenicity

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Progress in Natural Science:Materials International 2006年第12期16卷 1288-1294页

作者： XU Rongqi~ 1,3 , LI Xianzhen~3, WEI Hongyu~3, JIANG Bole~2, LI Kai~3, HE Yongqiang~ 2,3 , FENG Jiaxun~ 1,3 and TANG Jiliang~ 1,2 **(1. guangxi key laboratory of Subtropical Bioresources conservation and utilization 2. The key laboratory of Ministry of Education for Microbial and Plant Genetic Engineering 3. College of Life Science and Technology, guangxi University, Nanning 530004, China) [a]guangxi key laboratory of Subtropical Bioresources conservation and utilization [b]College of Life Science and Technology Guangxi University Nanning China [c]The key laboratory of Ministry of Education for Microbial and Plant Genetic Engineering

Eight putative avirulence genes in Xanthomonas campestris pv. campestris (Xcc) strain 8004 were characterized by Tn5gusA5 mutagenesis and gene expression analysis. The virulence test of mutants on Chinese radish showed that all mutants in individual avr genes except avrBs2 mutant were not significantly different from the wild type in virulence. The avrBs2 mutant showed reduced virulence and bacterial growth in planta. Gene expression analysis using β-glucuronidase as reporter indicated that avrBs1.1,avrBs1,avrXccB,avrXccC,avrXccE1 were regulated by hrpG, whereas avrXccA1, avrXccA2 and avrBs2 were not. RT-PCR analysis showed that all hrpG-regulated genes except avrBs1 were also regulated by hrpX. In addition, it was demonstrated that avrBs1 was responsible for elicitation of a type III dependent hypersensitive reaction (HR) on nonhost plant pepper ECW-10R, and wild type Xcc 8004 was unable to cause HR on pepper ECW-20R.

关键词： Xanthomonas campestris pv. campestris avirulence gene virulence expression analysis.

Polyp-Canal Reconstruction Reveals Evolution Toward Complexity in Corals

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Research 2024年第1期2023卷 417-428页

作者： Yixin Li Xin Liao Xin Wang Yuanchao Li Hongwei Zhao Yunpeng Zhao Junyuan Chen Chunpeng He Zuhong Lu State key laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China State key laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian 116024China guangxi key Lab of mangrove conservation and utilization Guangxi Mangrove Research CenterGuangxi Academy of SciencesBeihai 536000China Hainan Academy of Ocean and Fishery Sciences Haikou 571126China State key laboratory of Marine Resources utilization in South China Sea Hainan UniversityHaikou 570228China Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences39 East Beijing RoadNanjing 210008China

Modern scleractinian corals are classified into robust,complex,and basal clades through comparative molecular ***,only few morphological or biological criteria can systematically determine the evolutionary trajectories of these major scleractinian coral ***,we obtained the structural information of 21 scleractinian coral species representing robust and complex clades:High-resolution micro-computed tomography was used to reconstruct the polyp-canal systems in their colonies and to visualize the dynamic polyp growth *** found that the emergence of mesh-like canals may distinguish representatives of complex and robust *** differences in polyp-canal connections suggest distinct evolutionary trajectories among coral species:The formation of the canal network promoted the development of more complex coral structures,and coral polyps within this network formed calices of very similar volume,following precise axial growth *** influence of individual polyps on the coral colony becomes less significant as coral structures become more complex,and coral species with more complicated polyp-canal systems occupied niches more *** work supplements current evolutionary studies on reef-building corals,providing insight for further studies on coral growth patterns.

关键词： Coral representing directions

Lipid concentration and composition in xylem sap of woody angiosperms from a tropical savanna and a seasonal rainforest

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Plant Diversity 2024年第1期46卷 126-133页

作者： Ling-Bo Huang Xinyi Guan Amy Ny Aina Aritsara Jun-Jie Zhu Steven Jansen Kun-Fang Cao State key laboratory for conservation and utilization of Subtropical Agro-bioresources Guangxi Key Laboratory of Forest Ecology and ConservationGuangxi UniversityDaxuedong Road 100NanningGuangxi 530004China Institute of Botany Ulm UniversityAlbert-Einstein-Allee 11Ulm89081Germany

Lipids may play an important role in preventing gas embolisms by coating nanobubbles in xylem *** studies on xylem sap lipids have been reported for temperate plants,and it remain unclear whether sap lipids have adaptational significance in tropical *** this study,we quantify the lipid composition of xylem sap for angiosperm species from a tropical savanna(seven species)and a seasonal rainforest(five species)using mass *** found that all twelve species studied contained lipids in their xylem sap,including galactolipids,phospholipids and triacylglycerol,with a total lipid concentration ranging from 0.09 to 0.26 nmol/*** was no difference in lipid concentration or composition between plants from the two sites,and the lipid concentration was negatively related to species’open vessel ***,savanna species showed little variation in lipid composition between the dry and the rainy *** results support the hypothesis that xylem sap lipids are derived from the cytoplasm of individual conduit cells,remain trapped inside individual conduits,and undergo few changes in composition over consecutive seasons.A xylem sap lipidomic data set,which includes 12 tropical tree species from this study and 11 temperate tree species from literature,revealed no phylogenetic signals in lipid composition for these *** study fills a knowledge gap in the lipid content of xylem sap in tropical trees and provides additional support for their common distribution in xylem sap of woody *** appears that xylem sap lipids have no adaptive significance.

关键词： Xylem sap Lipids Tropical savanna Tropical rainforest Vessels