Construction and characterization of a hrpG mutant rendering constitutive expression of hrp genes in Xanthomonas campestris ***
Construction and characterization of a hrpG mutant rendering constitutive expression of hrp genes in Xanthomonas campestris ***作者机构：[a]The Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization [b]College of Life Science and Technology of Guangxi University Nanning China
出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))
年 卷 期：2006年第16卷第5期
页 面：480-485页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
主 题：hrpG Xanthomonas campestris pv. campestris effector constitutive expression site-specific mutagenesis.
摘 要：The expression of hrp genes in Xanthomonas is regulated by hrpG, an OmpR-type transcriptional activator, and can be induced by artificial mimic medium XVM2, but repressed in rich medium NYG. In this study, a site-specific mutation of hrpG in Xanthomonas campestris pv. campestris (Xcc) was generated by PCR-directed point mutagenesis and was characterized by its phenotype and transcriptional expression analyses. The results indicated that the E45K mutated hrpG rendered all the hrp genes expressing constitutively in the suppression medium NYG. Plant test showed that mutated hrpG enhanced the timing and intensity of hypersensitive reaction (HR) on the nonhost plant pepper, but did not affect the growth of Xcc in both culture medium and plants. The E45K mutation did not cause significant changes of production of exopolysaccharide, extracellular cellulase and amylase, but it inhibited the secretion of extracellular protease. This mutant 8004 is more suitable than the wild type for identifying hrp-dependent effectors in vitro and studying the transcriptional regulation genome-wide.
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