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Interaction between Heparin and Fibronectin: Using Quartz Crystal Microbalance with Dissipation, Immunochemistry and Isothermal Titration Calorimetry

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Journal of Wuhan University of Technology(Materials Science) 2015年第5期30卷 1074-1084页

作者：李贵才 WANG Caiping 杨苹 ZHOU Jie ZHU Pingchuan Jiangsu key laboratory of Neuroregeneration Nantong University key laboratory for Advanced Technologies of Materials Ministry of Education Southwest Jiaotong University guangxi key laboratory for subtropical bio-resource conservation and utilization Guangxi University

The adsorption behavior of heparin and fibronectin was studied by quartz crystal microbalance with dissipation(QCM-D), and the interaction between heparin and fibronectin was evaluated using immunochemistry and isothermal titration calorimetry(ITC) measurement. The results showed that there was competitive adsorption between heparin and fibronectin, and the preadsorption of fibronectin could prevent subsequent heparin adsorption to some extent, and the adsorbed Hep/Fn complex on the surface was in a rigid form. The bioactivity of heparin and fibronectin could be affected by the bulk concentration of each, and both heparin and fibronectin in Hep/Fn complex formed under p H 4 condition displayed larger bioactivity than that formed under p H 7 condition. Moreover, the fibronectin showed more exposed cell-binding sites at the p H value lower than physiological condition. The results of ITC further suggested that the interaction between heparin and fibronectin under p H 4 was stronger than under p H 7, and the complex was also more stable. The study brings forth the detailed interaction between heparin and fibronectin, which will be helpful for better understanding the interaction mechanism of the two biomolecules. The results may be potentially useful for the development of new generation of cardiovascular biomaterials.

关键词： Heparin fibronectin QCM-D immunochemistry isothermal titration calorimetry

Construction and characterization of a hrpG mutant rendering constitutive expression of hrp genes in Xanthomonas campestris ***

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Progress in Natural Science:Materials International 2006年第5期16卷 480-485页

作者： JIANG Bole, XU Rongqi, LI Xianzhen, WEI Hongyu, BAI Faan, HU Xi, HE Yongqiang and TANG Jiliang ( The guangxi key laboratory of subtropical bioresources conservation and utilization,College of Life Science and Technology of guangxi University, Nanning 530004, China) [a]The guangxi key laboratory of subtropical bioresources conservation and utilization [b]College of Life Science and Technology of guangxi University Nanning China

The expression of hrp genes in Xanthomonas is regulated by hrpG, an OmpR-type transcriptional activator, and can be induced by artificial mimic medium XVM2, but repressed in rich medium NYG. In this study, a site-specific mutation of hrpG in Xanthomonas campestris pv. campestris (Xcc) was generated by PCR-directed point mutagenesis and was characterized by its phenotype and transcriptional expression analyses. The results indicated that the E45K mutated hrpG rendered all the hrp genes expressing constitutively in the suppression medium NYG. Plant test showed that mutated hrpG enhanced the timing and intensity of hypersensitive reaction (HR) on the nonhost plant pepper, but did not affect the growth of Xcc in both culture medium and plants. The E45K mutation did not cause significant changes of production of exopolysaccharide, extracellular cellulase and amylase, but it inhibited the secretion of extracellular protease. This mutant 8004 is more suitable than the wild type for identifying hrp-dependent effectors in vitro and studying the transcriptional regulation genome-wide.

关键词： hrpG Xanthomonas campestris pv. campestris effector constitutive expression site-specific mutagenesis.

A Novel Sesquiterpene Isolated from Stereum sp.8954

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Chinese Chemical Letters 2005年第12期16卷 1615-1617页

作者： Guo Hong LI Ke Qin ZHANG laboratory for conservation and utilization of bio-resource Yunnan University Kunming 650091

Stereumone A, a novel sesquiterpene was isolated from the fermentation broth of Stereum sp. 8954. Its structure was determined to be 5-hydroxy-6,6,9-trimethyl-4,8a-epoxy- 2,3,4a,5,6,7,8a,9-octahydro-4H-naphtho[2,3-b]f... 详细信息

关键词： Stereumone A sesquiterpene Stereum sp

Structural and biochemical basis of FLS2-mediated signal activation and transduction in rice

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Plant Communications 2024年第3期5卷 216-231页

作者： Qiaoqiao Zhao Jinlin Bao Huailong Li Wei Hu Yanqiong Kong Yifeng Zhong Qiang Fu Guolyu Xu Fenmei Liu Xi Jiao Jian Jin Zhenhua Ming State key laboratory for conservation and utilization of subtropical Agro-bioresources College of Life Science and TechnologyGuangxi Key Laboratory for Sugarcane BiologyGuangxi UniversityNanning 530004P.R.China

The receptor-like kinase FLAGELLIN-SENSITIVE 2(FLS2)functions as a bacterialflagellin receptor local-ized on the cell membrane of *** Arabidopsis,the co-receptor BRI1-ASSOCIATED RECEPTOR KI-NASE 1(BAK1)cooperates with FLS2 to detect theflagellin epitopeflg22,resulting in formation of a signaling complex that triggers plant defense ***,the co-receptor responsible for recog-nizing and signaling theflg22 epitope in rice remains to be determined,and the precise structural mecha-nism underlying FLS2-mediated signal activation and transduction has not been *** study pre-sents the structural characterization of a kinase-dead mutant of the intracellular kinase domain of OsFLS2(OsFLS2-KDD1013A)in complex with ATP or ADP,resolved at resolutions of 1.98 A˚and 2.09 A˚,*** analysis revealed that OsFLS2 can adopt an active conformation in the absence of phosphorylation,although it exhibits only weak basal catalytic activity for ***-quent investigations demonstrated that OsSERK2 effectively phosphorylates OsFLS2,which reciprocally phosphorylates OsSERK2,leading to complete activation of OsSERK2 and rapid phosphorylation of the downstream substrate receptor-like cytoplasmic kinases OsRLCK176 and *** mass spectrometry experiments,we successfully identied critical autophosphorylation sites on OsSERK2,as well as sites transphosphorylated by ***,we demonstrated the interaction between OsSERK2 and OsFLS2,which is enhanced in the presence *** evidence suggests that OsRLCK176 and OsRLCK185 may function downstream of the OsFLS2-mediated signaling *** study reveals the molecular mechanism by which OsFLS2 mediates signal transduction pathways in rice and provides a valuable example for understanding RLK-mediated signaling pathways in plants.

关键词： FLS2 active conformation kinase activation transphosphorylation signal transduction

Regulation of eight avr genes by hrpG and hrpX in Xanthomonas campestris pv. campestris and their role in pathogenicity

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Progress in Natural Science:Materials International 2006年第12期16卷 1288-1294页

作者： XU Rongqi~ 1,3 , LI Xianzhen~3, WEI Hongyu~3, JIANG Bole~2, LI Kai~3, HE Yongqiang~ 2,3 , FENG Jiaxun~ 1,3 and TANG Jiliang~ 1,2 **(1. guangxi key laboratory of subtropical bioresources conservation and utilization 2. The key laboratory of Ministry of Education for Microbial and Plant Genetic Engineering 3. College of Life Science and Technology, guangxi University, Nanning 530004, China) [a]guangxi key laboratory of subtropical bioresources conservation and utilization [b]College of Life Science and Technology Guangxi University Nanning China [c]The key laboratory of Ministry of Education for Microbial and Plant Genetic Engineering

Eight putative avirulence genes in Xanthomonas campestris pv. campestris (Xcc) strain 8004 were characterized by Tn5gusA5 mutagenesis and gene expression analysis. The virulence test of mutants on Chinese radish showed that all mutants in individual avr genes except avrBs2 mutant were not significantly different from the wild type in virulence. The avrBs2 mutant showed reduced virulence and bacterial growth in planta. Gene expression analysis using β-glucuronidase as reporter indicated that avrBs1.1,avrBs1,avrXccB,avrXccC,avrXccE1 were regulated by hrpG, whereas avrXccA1, avrXccA2 and avrBs2 were not. RT-PCR analysis showed that all hrpG-regulated genes except avrBs1 were also regulated by hrpX. In addition, it was demonstrated that avrBs1 was responsible for elicitation of a type III dependent hypersensitive reaction (HR) on nonhost plant pepper ECW-10R, and wild type Xcc 8004 was unable to cause HR on pepper ECW-20R.

关键词： Xanthomonas campestris pv. campestris avirulence gene virulence expression analysis.

Manganese toxicity-induced chlorosis in sugarcane seedlings involves inhibition of chlorophyll biosynthesis

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The Crop Journal 2022年第6期10卷 1674-1682页

作者： Shu Yang Guizhi Ling Qiuyue Li Ke Yi Xinlian Tang Muqing Zhang Xiaofeng Li State key laboratory for conservation and utilization of subtropical Agro-bioresources/guangxi key laboratory for Sugarcane biology College of AgricultureGuangxi UniversityNanning 530004GuangxiChina Institute for New Rural Development Guangxi UniversityNanning 530004GuangxiChina

Manganese(Mn)toxicity-induced leaf chlorosis limits crop production in acidic soils,but its underlying mechanisms remain *** effects of excessive Mn on chlorophyll(Chl)biosynthesis in sugarcane(Saccharum officinarum L.)leaves were *** Mn treatment,Chl concentration decreased with Mn accumulation and chlorosis appeared in expanding *** that,levels of the initial Chl precursor 5-aminolevulinic acid(ALA)and its downstream intermediates decreased,whereas magnesium-protoporphyrin IX monomethyl ester(MgPME)*** of Mn in leaves downregulated the ALA biosynthetic gene GluTR(encoding glutamyl-tRNA reductase)and MgPME conversion gene MgPMEC(encoding MgPME cyclase),upregulated the ALA biosynthesis inhibitor FLU(encoding FLUORESCENT),but had no significant effect on the expression of other Chl biosynthetic *** above Mn-induced changes of Chl precursors and expression of corresponding genes commenced before the Chl decline and leaf chlorosis,and were reversed by ALA ***,excessive Mn-induced chlorosis in sugarcane is mediated by a Chl-biosynthesis disorder resulting from the inhibition of ALA synthesis and MgPME conversion.

关键词： Sugarcane Manganese toxicity Chlorosis Chlorophyll biosynthesis Gene expression

Evolution and conservation genetics of pangolins

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Integrative Zoology 2024年第3期19卷 426-441页

作者： Tongtong GU Jingyang HU Li YU State key laboratory for conservation and utilization of bio-resource in Yunnan School of Life SciencesYunnan UniversityKunmingChina

Pangolins(Pholidota,Manidae)are classified as an evolutionarily distinct and globally endangered mammal due to their unique morphology(nail-like scales and a myrmecophagous diet)and being the victim of heavy poaching and worldwide *** such,pangolins serve as a textbook example for studying the special phenotypic evolutionary adaptations and conservation genetics of an endangered *** years have demonstrated significant advancements in the fields of molecular genetics and genomics,which have translated to a series of important research achievements and breakthroughs concerning the evolution and conservation genetics of *** review comprehensively presents the hitherto advances in phylogeny,adaptive evolution,conservation genetics,and conservation genomics that are related to pangolins,which will provide an ample understanding of their diversity,molecular adaptation mechanisms,and evolutionary *** addition,we highlight the priority of investigating species/population diversity among pangolins and suggest several avenues of research that are highly relevant for future pangolin conservation.

关键词： adaptive evolution conservation genetics conservation genomics pangolins phylogeny

Individual variation in buffalo somatic cell cloning efficiency is related to glycolytic metabolism

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Science China(Life Sciences) 2022年第10期65卷 2076-2092页

作者： Chan Luo Zhiqiang Wang Jinling Wang Feng Yun Fenghua Lu Jiayuan Fu Qingyou Liu Deshun Shi State key laboratory for conservation and utilization of subtropical Agro-bioresources Guangxi UniversityNanning 530005China College of Animal Science and Technology Guangxi UniversityNanning 530005China

Mammalian individuals differ in their somatic cell cloning efficiency,but the mechanisms leading to this variation is poorly *** we found that high cloning efficiency buffalo fetal fibroblasts(BFFs)displayed robust energy metabolism,looser chromatin structure,high H3 K9 acetylation and low heterochromatin protein 1α(HP1α)*** cloning efficiency BFFs had more H3 K9 ac regions near to the upstream of glycolysis genes by Ch IP-seq,and involved more openness loci related to glycolysis genes through *** expression of these glycolysis genes was also found to be higher in high cloning efficiency BFFs by q *** key enzymes of glycolysis,PDKs and LDH,were confirmed to be associated with histone acetylation and chromatin openness of *** of low cloning efficiency BFFs with PS48(activator of PDK1)resulted in an increase in the intracellular lactate production and H3 K9 acetylation,decrease in histone deacetylase activity and HP1αexpression,less condensed chromatin structure and more cloning embryos developing to *** results indicate that the cloning efficiency of buffalo somatic cells is associated with their glycolytic metabolism and chromatin structure,and can be improved by increasing glycolytic metabolism.

关键词： buffalo cloning efficiency chromatin openness histone acetylation glycolytic metabolism

Physiological,transcriptome and gene functional analysis provide novel sights into cadmium accumulation and tolerance mechanisms in kenaf

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Journal of Environmental Sciences 2024年第3期137卷 500-514页

作者： Shan Cao Meng Wang Jiao Pan Dengjie Luo Samavia Mubeen Caijin Wang Jiao Yue Xia Wu Qijing Wu Hui Zhang Canni Chen Muzammal Rehman Sichen Xie Ru Li Peng Chen guangxi key laboratory of Agro-environment and Agric-products safety Key Laboratory of Plant Genetics and BreedingCollege of AgricultureGuangxi UniversityNanning 530004China State key laboratory for conservation and utilization of subtropical Agro-bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004China

Kenaf is considered to have great potential for remediation of heavy metals in ***,studies on molecular mechanisms of root Cd accumulation and tolerance are still *** this study,two differently tolerant kenaf cultivars were selected as materials and the physiological and transcriptomic effects were evaluated under Cd *** study showed that 200μmol/L CdCl_(2) treatment triggered the reactive oxygen species(ROS)explosion and membrane lipid *** with the Cd-sensitive cultivar‘Z',the Cd-tolerant cultivar‘F'was able to resist oxidative stress in cells by producing higher antioxidant enzyme activities and increasing the contents of ascorbic acid(AsA)and glutathione(GSH).The root cell wall of‘F'exhibited higher polysaccharide contents under Cd treatment,providing more Cd-binding *** were 3,439 differentially expressed genes(DEGs)that were co-regulated by Cd treatment in two *** biosynthesis and plant hormone signal transduction pathways were significantly enriched by functional annotation *** associated with pectin,cellulose,and hemi-cellulose metabolism were involved in Cd chelation of root cell wall;V-ATPases,ABCC3 and Narmp3 could participated in vacuolar compartmentalization of Cd;PDR1 was responsible for Cd effux;the organic acid transporters contributed to the absorption of Cd in *** genes might have played key roles in kenaf Cd tolerance and Cd ***,HcZIP2 was identified to be involved in Cd uptake and transport in *** findings provide a deeper understanding of the molecular pathways underlying Cd accumulation and detoxification mechanisms in kenaf.

关键词： Kenaf Cd stress Physiological Transcriptome HcZIP2