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In vivo volumetric monitoring of revascularization of traumatized skin using extended depth-of-field photoacoustic microscopy

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Frontiers of Optoelectronics 2020年第4期13卷 307-317页

作者： Zhongwen CHENG Haigang MA Zhiyang WANG Sihua YANG MOE key laboratory of laser life science&Institute of laser life science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Faster and better wound healing is a critical medical issue.Because the repair process of wounds is closely related to revascularization,accurate early assessment and postoperative monitoring are very important for establishing an optimal treatment plan.Herein,we present an extended depth-of-field photoacoustic microscopy system(E-DOF-PAM)that can achieve a constant spatial resolution and relatively uniform excitation efficiency over a long axial range.The superior performance of the system was verified by phantom and in vivo experiments.Furthermore,the system was applied to the imaging of normal and trauma sites of volunteers,and the experimental results accurately revealed the morphological differences between the normal and traumatized skin of the epidermis and dermis.These results demonstrated that the E-DOF-PAM is a powerful tool for observing and understanding the pathophysiology of cutaneous wound healing.

关键词： photoacoustic microscopy(PAM) extended depth-of-field traumatized skin

Infrared Spectroscopy of Neutral and Cationic Pyrrolidine Monomer in Supersonic Jet

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Chinese Journal of Chemical Physics 2020年第1期33卷 43-47,I0005,I0006页

作者： Min Xie Zhao-li Zhang Yu Zhang Xiao-nan Sun Fu-fei Sun Yong-jun Hu MOE key laboratory of laser life science&Guangdong Provincial Key Laboratory of Laser Life Science Guangzhou Key Laboratory of Spectral Analysis and Functional ProbesCollege of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Pyrrolidine,a five membered heterocyclic molecule,is widely existing in organism.Herein,infrared spectra of pyrrolidine monomer in neutral and cationic states were obtained by vacuum ultraviolet ionization,infrared photodissociation and time of flight mass spectrometry.Both in neutral and cationic states,it is found that their CH stretching vibration bands are red shifted.In the IR spectrum of neutral pyrrolidine,because the electric dipole moment of NH is small,we have not observed the NH stretching vibration bands.However,the NH stretching vibration band of pyrrolidine is greatly enhanced after ionization,and this band red-shifts compared with the previous experiment.The red shifts of CH stretching vibrations in neutral and cationic states are caused by the negative and positive hyperconjugation,respectively.The enhancement and red shift of the NH stretching band are owing to the ejection of the electrons on the N atom after ionization.Through the calculations,it is found that the acidity of the CH bond is a little stronger than that of NH bond.These kinds of studies would be helpful to understand the intrinsic properties of biomolecules in neutral and cationic states,and to provide reference for the further study of living organic macromolecules.

关键词： IR spectroscopy Hyperconjugation Cationic state CH bond acidity Gas phase

Light-responsive charge-reversal nanovector for high-efficiency in vivo CRISPR/Cas9 gene editing with controllable location and time

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Nano Research 2020年第9期13卷 2399-2406页

作者： Yunxia Wu Judun Zheng Qin Zeng Tao Zhang Da Xing MOE key laboratory of laser life science&Institute of laser life science South China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Controllably and efficaciously localized CRISPR/Cas9 plasmids transfection plays an essential role in genetic editing associated with various key human diseases.We employed near-infrared(NIR)light-responsive CRISPR/Cas9 plasmids delivery via a charge-reversal nanovector to achieve highly efficient and site-specific gene editing.The nanovector with abundant positive charges was fabricated on the basis of an ultraviolet-sensitive conjugated polyelectrolyte coated on an upconversion nanomaterial(UCNP-UVP-P),which can convert into negative charges upon 980 nm light irradiation.Using the as-prepared nanovector,we demonstrated the plasmids could be efficiency transfected into tumor cells(~63%±4%)in a time-contolled manner,and that functional CRISPR/Cas9 proteins could be successfully expressed in a selected NIR-irradiated region.Particularly,this strategy was successfully applied to the delivery of CRISPR/Cas9 gene to tumor cells in vivo,inducing high efficiency editing of the target gene PLK-1 under photolrradiation.Therefore,this precisely controlled gene regulation strategy has the potential to serve as a new paradigm for gene engineering in complex biological systems.

关键词： light-responsive charge-reversal CRISPR/Cas9 gene editing

Hydrogen-rich water ameliorates neuropathological impairments in a mouse model of Alzheimer's disease through reducing neuroinflammation and modulating intestinal microbiota

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Neural Regeneration Research 2022年第2期17卷 409-417页

作者： Yi-Tong Lin Qing-Qing Shi Lei Zhang Cai-Ping Yue Zhi-Jun He Xue-Xia Li Qian-Jun He Qiong Liu Xiu-Bo Du College of life sciences and Oceanography Guangdong Provincial Key Laboratory for Plant EpigeneticsShenzhen UniversityShenzhenGuangdong ProvinceChina guangdong provincial key laboratory of Biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical EngineeringHealth Science CenterShenzhen UniversityShenzhenGuangdong ProvinceChina Shenzhen Bay laboratory ShenzhenGuangdong ProvinceChina

Hydrogen exhibits the potential to treat Alzheimer's disease. Stereotactic injection has been previously used as an invasive method of administering active hydrogen, but this method has limitations in clinical practice. In this study, triple transgenic(3×Tg) Alzheimer's disease mice were treated with hydrogen-rich water for 7 months. The results showed that hydrogen-rich water prevented synaptic loss and neuronal death, inhibited senile plaques, and reduced hyperphosphorylated tau and neurofibrillary tangles in 3×Tg Alzheimer's disease mice. In addition, hydrogen-rich water improved brain energy metabolism disorders and intestinal flora imbalances and reduced inflammatory reactions. These findings suggest that hydrogen-rich water is an effective hydrogen donor that can treat Alzheimer's disease. This study was approved by the Animal Ethics and Welfare Committee of Shenzhen University, China(approval No. AEWC-20140615-002) on June 15, 2014.

关键词： Alzheimer's disease amyloid-β anti-inflammation bioenergetics gut microbiota hydrogen therapy neurodegenerative disease neurofibrillary tangles

Aza-BODIPY-based phototheranostic nanoagent for tissue oxygen auto-adaptive photodynamic/photothermal complementary therapy

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Nano Research 2022年第1期15卷 716-727页

作者： Meijiao Zhao Qin Zeng Xipeng Li Da Xing Tao Zhang MOE key laboratory of laser life science&Institute of laser life science South China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Tumor oxygen spatial heterogeneity is a critical challenge for the photodynamic inhibition of solid tumors.Development of an intelligent nanoagent to initiate optimal therapeutics according to the localized oxygen levels is an effective settlement.Herein,we report an activatable nanoagent(BDP-Oxide nanoparticles(NPs))to enable the oxygen auto-adaptive photodynamic/photothermal complementaly treatment.Upon the nanoagent accumulated in the tumor region,the low extracellular pH could trigger the disassociation of the nanoagent to release the phototheranostic agent,BDP-Oxide,which will subsequently afford the fluorescence imaging-guided photodynamic oxidation after it gets into the outer oxygen-rich tumors.Along with the penetration deepening in the solid tumor,furthermore,BDP-Oxide could be reduced into BDP by the cytochrome P450(CYP450)enzymes activated in the low oxygen tension regions of inner hypoxic tumors,which will switch on the photothermal and photoacoustic effects.Overall,the BDP-Oxide NPs-enabled photodynamic/photothermal complementary therapy significantly suppressed the solid tumor growth(inhibition rate of 94.8%).This work proposes an intelligent platform to address the oxygen partial pressure for the optimization of cancer phototherapeutics.

关键词： boron dipyrromethene(BODIPY) complementary therapy self-adaptive spatial heterogeneity of oxygen

Calcium-dependent activation of CPK12 facilitates its cytoplasm-to-nucleus translocation to potentiate plant hypoxia sensing by phosphorylating ERF-Ⅶ transcription factors

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Molecular Plant 2023年第6期16卷 979-998页

作者： Biao Fan Ke Liao Lin-Na Wang Li-Li Shi Yi Zhang Ling-Jing Xu Ying Zhou Jian-Feng Li Yue-Qin Chen Qin-Fang Chen Shi Xiao State key laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resourceschool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Calcium-dependent protein kinases(CDPKs/CPKs)are key regulators of plant stress signaling that translate calcium signals into cellular responses by phosphorylating diverse substrate proteins.However,the molecular mechanism by which plant cells relay calcium signals in response to hypoxia remains elusive.Here,we show that one member of the CDPK family in Arabidopsis thaliana,CPK12,is rapidly activated during hypoxia through calcium-dependent phosphorylation of its Ser-186 residue.Phosphorylated CPK12 shuttles from the cytoplasm to the nucleus,where it interacts with and phosphorylates the group Ⅶ ethylene-responsive transcription factors(ERF-Ⅶ)that are core regulators of plant hypoxia sensing,to enhance their stabilities.Consistently,CPK12 knockdown lines show attenuated tolerance of hypoxia,whereas transgenic plants overexpressing CPK12 display improved hypoxia tolerance.Nonethelss,loss of function of five ERF-Ⅶ proteins in an erf-vii pentuple mutant could partially suppress the enhanced hypoxia-tolerance phenotype of CPK12-overexpressing lines.Moreover,we also discovered that phosphatidic acid and 14-3-3κ protein serve as positive and negative modulators of the CPK12 cytoplasm-to-nucleus translocation,respectively.Taken together,these findings uncover a CPK12-ERF-Ⅶ regulatory module that is key to transducing calcium signals from the cytoplasm into the nucleus to potentiate hypoxia sensing in plants.

关键词： CPK12 hypoxia sensing cytoplasm-to-nucleus translocation ERF-Ⅶtranscription factors phospha-tidic acid 14-3-3 proteins

A positive feedback regulation of SnRK1 signaling by autophagy in plants

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Molecular Plant 2023年第7期16卷 1192-1211页

作者： Chao Yang Xibao Li Lianming Yang Shunquan Chen Jun Liao Kailin Li Jun Zhou Wenjin Shen Xiaohong Zhuang Mingyi Bai Diane CBassham Caiji Gao guangdong provincial key laboratory of Biotechnology for Plant Development Ministry of Education&Guangdong Provincial Key Laboratory of Laser Life ScienceSchool of Life SciencesSouth China Normal UniversityGuangzhou 510631China guangdong provincial key laboratory of Applied Botany&key laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China Centre for Cell and Developmental Biology and State key laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong KongShatinNewTerritoriesHongKongChina The key laboratory of Plant Development and Environmental Adaptation Biology Ministry of EducationSchool of Life SciencesShandong UniversityQingdao 266237China Department of Genetics Development and Cell Biologylowa State UniversityAmesIAUSA

SnRK1,an evolutionarily conserved heterotrimeric kinase complex that acts as a key metabolic sensor in maintaining energy homeostasis in plants,is an important upstream activator of autophagy that serves as a cellular degradation mechanism for the healthy growth of plants.However,whether and how the autophagy pathway is involved in regulating SnRK1 activity remains unknown.In this study,we identified a clade of plant-specific and mitochondria-localized Fcs-like zinc finger(FLZ)proteins as currently unknown ATG8-interacting partners that actively inhibit SnRK1 signaling by repressing the T-loop phosphorylation of the catalyticαsubunits of SnRK1,thereby negatively modulating autophagy and plant tolerance to energy deprivation caused by long-term carbon starvation.Interestingly,these AtFLZs are transcriptionally repressed by low-energy stress,and AtFLZ proteins undergo a selective autophagy-dependent pathway to be delivered to the vacuole for degradation,thereby constituting a positive feedback regulation to relieve their repression of SnRK1 signaling.Bioinformatic analyses show that the ATG8-FLZ-SnRK1 regulatory axis first appears in gymnosperms and seems to be highly conserved during the evolution of seed plants.Consistent with this,depletion of ATG8-interacting ZmFLZ14 confers enhanced tolerance,whereas overexpression of ZmFLZ14 leads to reduced tolerance to energy deprivation in maize.Collectively,our study reveals a previously unknown mechanism by which autophagy contributes to the positive feedback regulation of SnRK1 signaling,thereby enabling plants to better adapt to stressful environments.

关键词： snrk1 autophagy ATG8 FLZ carbon starvation

Collagen fiber anisotropy characterization by polarized photoacoustic imaging for just-in-time quantitative evaluation of burn severity

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Photonics Research 2023年第5期11卷 817-828页

作者： ZHENHUI ZHANG WEI CHEN DANDAN CUI JIE MI GEN MU LIMING NIE SIHUA YANG YUJIAO SHI MOE key laboratory of laser life science&Institute of laser life science South China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China School of Medicine South China University of TechnologyGuangzhou 510006China Research Center of Medical sciences Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China

Just-in-time burn severity assessment plays a vital role in burn treatment and care.However,it is still difficult to quantitatively and promptly evaluate burn severity by existing medical imaging methods via initial burn depth measurement since burn wounds are usually dynamically developed.As an elastic skeleton of skin,the degree of conformational changes of collagen fibers caused by overheating can reflect the burn severity in a timelier manner.Herein,the polarized photoacoustic technique(PPAT)for just-in-time quantitative evaluation of burn severity via collagen fiber anisotropy assessment is proposed.First,phantom experiments demonstrate the ability of PPAT for deep imaging in a transport mean free path and accurately quantify changes in microstructural order by thermal damage.Then,the Pearson correlation coefficient of the PPAT in assessing burn severity is shown to be up to 0.95,validated by burn skin samples.The PPAT provides a just-in-time quantitative strategy for burn severity evaluation.

关键词： severity anisotropy polarized

Whole-genome sequencing of leopard coral grouper(Plectropomus leopardus) and exploration of regulation mechanism of skin color and adaptive evolution

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Zoological Research 2020年第3期41卷 328-340页

作者： Yang Yang Li-Na Wu Jing-Fang Chen Xi Wu Jun-Hong Xia Zi-Ning Meng Xiao-Chun Liu Hao-Ran Lin State key laboratory of Biocontrol Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic AnimalsLife Sciences SchoolSun Yat-Sen UniversityGuangzhouGuangdong 510275China State key laboratory of Biocontrol and guangdong provincial key laboratory of Plant Resources School of Life SciencesSun Yat-Sen UniversityGuangzhouGuangdong 510275China Southern laboratory of Ocean science and Engineering ZhuhaiGuangdong 519000China

Leopard coral groupers belong to the Plectropomus genus of the Epinephelidae family and are important fish for coral reef ecosystems and the marine aquaculture industry. To promote future research of this species, a high-quality chromosome-level genome was assembled using PacBio sequencing and Hi-C technology. A 787.06 Mb genome was assembled, with 99.7%(784.57 Mb) of bases anchored to 24 chromosomes. The leopard coral grouper genome size was smaller than that of other groupers, which may be related to its ancient status among grouper species. A total of 22 317 proteincoding genes were predicted. This high-quality genome of the leopard coral grouper is the first genomic resource for Plectropomus and should provide a pivotal genetic foundation for further research. Phylogenetic analysis of the leopard coral grouper and 12 other fish species showed that this fish is closely related to the brown-marbled grouper.Expanded genes in the leopard coral grouper genome were mainly associated with immune response and movement ability, which may be related to the adaptive evolution of this species to its habitat. In addition, we also identified differentially expressed genes(DEGs) associated with carotenoid metabolism between red and brown-colored leopard coral groupers. These genes may play roles in skin color decision by regulating carotenoid content in these groupers.

关键词： Leopard coral grouper Genome Evolution Skin color Immune