检索结果-南通市图书馆

Effects of hydroxyproline supplementation in low fish meal diets on collagen synthesis, myofiber development and muscular texture of juvenile Pacific white shrimp(Litopenaeus vannamei)

引用

Animal Nutrition 2024年第2期17卷 428-437页

作者： Menglin Shi Haoming Li Tianyu Chen Bocheng Huang Xiaoyue Li Xiaohui Dong Shuyan Chi Qihui Yang Hongyu Liu Junming Deng Beiping Tan Shuang Zhang Shiwei Xie laboratory of aquatic Nutrition and Feed College of Fisheries Guangdong Ocean University aquatic animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of guangdong Province key laboratory of aquatic Livestock and Poultry Feed Science and Technology in South China Ministry of Agriculture guangdong provincial key lab of aquatic animals disease control and healthy culture

This experiment aimed to evaluate the impact of dietary hydroxyproline(Hyp) supplementation on the muscle quality of juvenile Pacific white shrimp(Litopenaeus vannamei) fed a low fishmeal diet. Six formulated diets included one high fishmeal(HF; 25% fishmeal content) and five low fishmeal diets(10%fishmeal content) with 0%, 0.2%, 0.4%, 0.6% and 0.8% Hyp(LF0, LF2, LF4, LF6 and LF8, respectively). Each diet was assigned to four replicates, and 40 shrimp(0.32 ± 0.00 g) per replicate were fed four times a day for 8 weeks. Dietary Hyp supplementation had little effects on growth performance, but increased the contents of Hyp, prolyl 4-hydroxylases(P4Hs), and collagen. The meat yield, springiness, hardness,chewiness, and cohesiveness of muscle were the highest in the LF4 group among the low fishmeal groups(P < 0.05). Cooking loss and freezing loss of muscle were the lowest in the LF4 group(P < 0.05). Dietary supplementation with 0.4% Hyp increased the myofiber density and decreased the myofiber diameter of muscle(P < 0.05). Supplementation of Hyp in the diet up-regulated the mRNA expression of smyhc5,smyhc15, col1a1, col1a2, igf-1f, tgf-β and tor and down-regulated the mRNA expression of smyhc1, smyhc2,smyhc6a(P < 0.05). Supplementation of Hyp in the diet up-regulated the protein expression of P-4E-BP1,P-AKT, AKT and P-AKT/AKT(P < 0.05). These results suggested that the addition of 0.4% Hyp to low fishmeal diets improved the muscle quality of L. vannamei.

关键词： Hydroxyproline Collagen Muscle quality Litopenaeus vannamei

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Molecular Cloning and Bioinformatics Analysis of sucC Gene of Vibrio alginolyticus Strain HY9901

引用

Asian Agricultural Research 2024年第8期16卷 32-37页

作者： Yingzhu WEI Zhiqing WEI Xuelian LIN Huanying PANG Na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal disease control and healthy culture&key laboratory of control for diseases of aquatic Economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis.[Methods]Based on the sucC gene of *** strain HY9901,specific primers were designed to amplify the full length sequence by PCR and make further analysis.[Results]The theoretical molecular weight of SucC protein was about 41528.45 Da,and the full length was 1167 bp,encoding 388 amino *** has no signal peptide and transmembrane region,and has a variety of functional *** is predicted that it is mainly located in the cytoplasm,and the ubiquitin and lactate modification sites overlap,and it has high gene homology with Vibrio ***α-helix,random coil and extended strand are the main secondary *** similarity between the constructed three-level structure model and the template is high.[Conclusions]This study reveals the structural characteristics and functional potential of SucC protein,and provides a theoretical basis for the study of drug resistance mechanism and prevention strategies.

关键词： Vibrio alginolyticus Gene amplification sucC gene Succinyl-Coa synthetase Protein post-translational modification Bioinformatics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Cloning and Bioinformatics Analysis of msrA Gene from Vibrio alginolyticus Strain HY9901

引用

Agricultural Biotechnology 2024年第4期13卷 1-5,10页

作者： Zhiqing WEI Zhihang CHEN Yingzhu WEI Na WANG Huanying PANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal disease control and healthy culture&key laboratory of control for diseases of aquatic Economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]This study was conducted to understand the structure and function of MsrA protein.[Methods]With Vibrio alginolyticus HY9901 as the object of study,primers were designed to amplify the full-length gene of msrA,and its bioinformatics analysis was carried out.[Results]The full length of msrA gene was 639 bp,encoding 212 amino acids,and its theoretical molecular weight was about 23729.60 *** protein had a stable structure,and it was hydrophobic *** structure of signal peptides at the N terminal of the amino acid sequence was predicted,and it was found that there was no signal peptide cleavage site and no transmembrane *** amino acid sequence of MsrA contained multiple signal binding *** subcellular localization showed that MsrA protein was most likely located in the *** analysis showed that MsrA of *** had high homology with other Vibrio species,and the highest homology with *** the prediction of functional domains,MsrA had the function of methionine sulfoxide *** secondary structure prediction,MsrA contained random coils at a proportion of 46.70%,which was the *** similarity between the tertiary structure model of MsrA and template Q87SW6.1.A was 89.15%.PTM analysis showed that MsrA protein had many PTM modification sites such as phosphorylation and glycosylation sites.[Conclusions]This study provides some reference value for further study on the role of MsrA in bacterial antioxidant stress.

关键词： Vibrio alginolyticus Gene cloning MsrA Bioinformatics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Cloning of clpX Gene from Vibrio alginolyticus HY9901 and Its Bioinformatics Analysis

引用

Asian Agricultural Research 2024年第8期16卷 17-22页

作者： Xiaoxin WEN Yuyan HE Jiajie MA Weijie ZHANG Huanying PANG Na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524088China guangdong provincial key laboratory of aquatic Animal disease control and healthy culture&key laboratory of control for diseases of aquatic Economic animals of guangdong Higher Education Institutes Zhanjiang 524088China Chinese Academy of Inspection and Quarantine Beijing 100176China

According to the clpX gene sequence of Vibrio alginolyticus HY9901,a pair of specific primers were designed,and the full length was cloned by PCR and subjected to bioinformatics *** results showed that the clpX gene was 1281 bp in length and encoded 426 amino *** molecular structure formula was C 3842 H 6405 N 1281 O 1598 S 260,with a theoretical protein molecular weight of approximately 1044473.4 kDa and a theoretical pI value of *** clpX gene was predominantly situated within the cytoplasm,exhibiting unstable and hydrophilic protein *** possessed a signal peptide cleavage site,lacked a transmembrane region,and was not associated with any KEGG metabolic ***,it possessed 2 glycine phosphorylation sites,a CAMP-dependent protein kinase phosphorylation site,a C-terminal amidation modification site,6 protein kinase C phosphorylation sites,7 microbody C-terminal target signal sites,and an ATP/GTP *** clpX phylogenetic tree was constructed using the MEGA 5.0 software via the neighbor-joining *** results demonstrated that the clpX of *** exhibited up to 100%affinity with the clpX of Vibrio *** single subunit 3D structure model of the ClpX protein was obtained using the SWISS-MODEL program.A structural and functional analysis of the protein revealed the presence of three distinct ClpX structural and functional *** the prediction of secondary structure,the proportions ofα-helix,random coil,β-sheet and extended strand were 40.38%,37.09%,5.40%and 17.14%,*** analysis of the ClpX protein through the STRING database revealed that the proteins interacting with the ClpX protein were Tig,Atpd,Hflb,Msrb-2,Rpod,Clpp,Clpa,Lon-1,Hfq,and ANP63951.1.A computational analysis of the ClpX protein identified a number of post-translational modification sites,including phosphorylation,acetylation,ubiquitination,glycosylation,methylation,S-palmitoylation,and *** significance of this st

关键词： Vibrio alginolyticus clpX gene Bioinformatics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Amplification and Bioinformatics Analysis of vscN Gene from Vibrio alginolyticus

引用

Asian Agricultural Research 2023年第5期15卷 29-33页

作者： Linlin YIN Wen LI Haiyun FENG Weijie ZHANG Junlin WANG Huanying PANG Na WANG College of Fisheries Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal disease control and healthy culture/key laboratory of control for diseases of aquatic Economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]The paper was to clone and analyze bioinformatics of vscN gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the vscN gene sequence of *** *** full length of vscN gene was amplified by PCR and bioinformatics analysis was performed.[Results]The vscN gene was 1323 bp in total,encoding 440 amino acids,with the theoretical molecular weight of 47.86 kD and the theoretical pI value of *** online prediction showed that there was no signal peptide and no transmembrane region in *** amino acid sequence had 10 N-myristoylation sites,8 phosphorylation sites(2 protein kinase C phosphorylation sites,6 casein kinase II phosphorylation sites),1 amidation site,11 microbody C-terminal target signal sites,1 ATP/GTP binding site motif A(P ring),and 1 ATPaseαandβsubunit specific *** analysis showed that the VscN protein of *** had high homology with that of ***,with a similarity of 95.14%.Phylogenetic tree analysis showed that the VscN of *** was clustered into the same subgroup as that of *** and *** domain analysis of VscN protein showed that it had Pfam and AAA domains,and involved in the regulation of bacterial *** three-dimensional structure model of VscN simulated by SWISS-MODEL software was similar to the structure of flagellate-specific ATPase FliH-FliI complex.[Conclusions]The results lay a foundation for further study on the regulatory mechanism of VscN protein on bacterial virulence.

关键词： Vibrio alginolyticus T3SS VscN Bioinformatics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cloning and Bioinformatics Analysis of hcp Gene in Aeromonas hydrophila

引用

Asian Agricultural Research 2024年第3期16卷 36-40页

作者： Fan LI Xingjun FU Haiyun FENG Shi WANG Weijie ZHANG Huanying PANG Na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal disease control and healthy culture&key laboratory of control for diseases of aquatic Economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]To explore the function of hcp gene in Aeromonas hydrophila.[Methods]A pair of specific primers was designed referring to the hcp gene sequence of *** hcp gene was amplified by PCR,and performed bioinformatics analysis.[Results]The hcp gene had a total length of 1650 bp and encoded 549 amino *** theoretical molecular weight of the protein predicted was about 59476.44 *** predicting the N-terminal signal peptide structure of the amino acid sequence,neither obvious signal peptide cleavage site nor signal peptide was found,and the protein had no transmembrane *** amino acid sequence had a N-glycosylation site,4 protein kinase C phosphorylation sites,7 casein kinase II phosphorylation sites,9 N-myristoylation sites,4 isoprene binding sites,10 microbody C-terminal target signal sites,and an ATP/GTP binding site motif A(P-ring).The amino acid sequence of hcp gene of *** was performed homology analysis with other Aeromonas strains,and it showed higher homology with *** the secondary structure,theα-helix,β-sheet,random coil and extended strand accounted for 45.36%,6.01%,37.52%and 11.11%,*** tertiary structure model consisted of 18α-helix and 22β-*** of protein-protein network interaction demonstrated that the proteins interacting with Hcp protein were AHA_3407,nrfA,nirB-1,nirB-2 and AHA_1112.[Conclusions]Through the bioinformatics prediction results,the basic information of hcp gene of *** is preliminarily understood,and the possible function of this protein is predicted,in order to provide guidance for subsequent vaccine research.

关键词： Aeromonas hydrophila Gene amplification hcp gene Bioinformatics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Amplification and Bioinformatics Analysis of h-ns Gene of Vibrio alginolyticus

引用

Asian Agricultural Research 2023年第4期15卷 29-33页

作者： Ying CHEN Shi WANG Liangchuan CHEN Haiyun FENG Junlin WANG Huanying PANG Na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal disease control and healthy culture&key laboratory of control for diseases of aquatic Economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]To amplify the h-ns gene of Vibrio alginolyticus and analyze it by bioinformatics.[Methods]According to the h-ns gene sequence of *** HY9901,a pair of specific primers were designed and amplified by PCR.[Results]The h-ns gene was 408 bp in length and 135 amino acids were *** predicted theoretical protein molecular weight was about 14.98 kD,and the isoelectric point was *** subcellular localization,SignalP 5.0,TMHMM Server 2.0 and SoftBerry-Psite predictions showed that H-NS was located outside the cell membrane,and the protein was unstable and *** was no signal peptide cleavage site,no transmembrane region and no KEGG metabolic *** amino acid sequence contained three phosphorylation sites,one N-terminal myristoylation site and three microsomal C-terminal target signal *** MEGA 5.0,H-NS phylogenetic tree was constructed by ortho-connection *** results showed that H-NS of *** was closer to H-NS of Vibrio *** SWISS-MODEL,the three-dimensional structure model of H-NS subunit was simulated,which was similar to the crystal structure of Salmonella typhimurium H-NS1-83.[Conclusions]This study lays a foundation for exploring the regulation mechanism of *** H-NS protein on bacterial virulence in the future.

关键词： Vibrio alginolyticus Gene cloning H-NS Bioinformatics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Cloning and Bioinformatics Analysis of cyaA Gene of Vibrio alginolyticus Strain HY9901

引用

Agricultural Biotechnology 2024年第4期13卷 6-10页

作者： Shi WANG Peng ZHOU Yujia ZHANG Weijie ZHANG Na WANG Huanying PANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal disease control and healthy culture&key laboratory of control for diseases of aquatic Economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]This study was conducted to explore the biological functions of cyaA gene of Vibrio alginolyticus.[Methods]With DNA of *** HY 9901 as a template,primers were designed according to the sequence of cyaA gene,and the cyaA gene was amplified by *** analysis was performed.[Results]The cyaA gene of *** HY9901 was 2529 bp in size,and encoded 842 amino *** molecular structure of CyaA protein was C_(4358)H_(6745)N_(1171)O_(1286)S_(35).Its theoretical molecular weight was 97.24167 kDa and the theoretical pI value was *** had no signal peptide and transmembrane *** protein had three N-terminal glycosylation sites,one cAMP and cGMP-dependent protein kinase phosphorylation site,nine protein kinase C phosphorylation sites,nine casein kinase II phosphorylation sites,one tyrosine kinase phosphorylation site,seven N-terminal myristoylation sites,one pentenyl binding site and ten microbody C-terminal localization signal *** localization prediction showed that CyaA protein was mainly located in the nucleus and *** multi-sequence alignment and phylogenetic tree construction,it was concluded that *** had high CyaA homology with other Vibrio *** of *** was clustered with Vibrio fluminensis and Vibrio marinisedimini,and they were closely *** secondary structure of CyaA protein consisted ofα-helixes(43.11%),random coils(38.00%)and extended strands(14.49%).In protein network interaction,it was found that the proteins adjacent to CyaA protein were Crp-2,CpdA,Crr,PtsG-2,ANP67209.1,Crp-1,PykF,Pyk,RelA and Ndk.[Conclusions]This study provides a new idea for formulating strategies for the prevention and control of vibriosis.

关键词： Vibrio alginolyticus cyaA gene Bioinformatics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of Microplastics on Expression of Resistance Genes and Virulence Genes of Vibrio alginolyticus

引用

Asian Agricultural Research 2023年第2期15卷 42-45页

作者： Liangchuan CHEN Zehui SU Haiyun FENG Weijie ZHANG Huanying PANG Na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal disease control and healthy culture&key laboratory of control for diseases of aquatic Economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]To study the effects of microplastics on antibiotic resistance genes and virulence genes of Vibrio alginolyticus,so as to provide a certain reference for controlling marine pollution,curbing the spread of environmental antibiotic resistance genes and virulence genes,formulating environmental policies,and maintaining food safety.[Methods]After adding *** into the artificial seawater,they were divided into three groups,namely blank control group(BLK),polyvinyl chloride microplastic group(PVC group)and polyvinyl alcohol microplastic group(PVA group).Aerated culture experiments were carried out,and the effects of microplastics on the expression of resistance genes and virulence genes of *** were studied by PCR and qPCR methods.[Results]The presence of microplastics significantly changed the resistance gene structure of *** with the control group,the cfxA and cfr resistance genes were detected in the microplastic ***,only PVC group detected blaZ resistance gene,and only PVA group did not detect aaC resistance *** addition,compared with the control group,the expressions of virulence genes in the microplastic group were all down-regulated(P<0.01).[Conclusions]This study provides some reference for curbing the spread of environmental antibiotic resistance genes and virulence genes,formulating environmental policies,and maintaining food safety,but the specific mechanisms of drug resistance and virulence need further research.

关键词： Vibrio alginolyticus Microplastics Resistance genes Virulence genes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cloing and Bioinformatics Analysis of ndk Gene from Vibrio alginolyticus

引用

Asian Agricultural Research 2023年第7期15卷 30-34页

作者： Yujia ZHANG Shi WANG Jian ZHONG Weijie ZHANG Xing XIAO Zhiqing WEI Huanying PANG Na WANG College of Fisheries Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal disease control and healthy culture/key laboratory of control for diseases of aquatic Economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Zhanjiang Customs Zhanjiang 524022China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]The paper was to clone and analyze bioinformatics of ndk gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the ndk gene sequence of *** *** full length of ndk gene was amplified by PCR and bioinformatics analysis was *** 5.0 software was used to construct NDK phylogenetic tree by neighbor-joining ***-MODEL program was used to obtain the three-dimensional structural model of single subunit from NDK protein.[Results]The ndk gene,molecular structural formula C702H1094N192O214S7,was 426 bp in total,encoding 141 amino acids,with the theoretical molecular weight of 15.87199 kD and the theoretical pI value of *** prediction results of protein subcellular localization,SignalP 5.0,TMHMM Server 2.0 and SoftBerry-Psite showed that NDK mainly existed in the cytoplasm,and the protein was unstable and *** was neither signal peptide cleavage site,nor transmembrane region and KEGG metabolic *** amino acid sequence had two protein kinase C phosphorylation sites,a casein kinaseⅡphosphorylation site,a N-myristoylation site,three microbody C-terminal target signal sites,and a nucleoside diphosphate kinase active *** analysis showed that the NDK of *** had high homology with that of ***,with a similarity of 98.58%.Analysis of the structural functional domain revealed that the protein had one NDK structural functional *** prediction results of secondary structure showed that theα-helix,random coil,β-sheet and extended strand accounted for 53.19%,28.37%,7.09%and 11.35%,*** of NDK protein via STRING database demonstrated that the proteins interacting with NDK protein were NrdA,NrdB,GmK,CmK,TmK,PyrG,PyrH,RelA,FolE and SpoT.[Conclusions]The study plays a positive role in the prevention and control of vibriosis and the improvement of the current aquaculture environment.

关键词： Vibrio alginolyticus Gene cloning ndk Bioinformatics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：