Cloing and Bioinformatics Analysis of ndk Gene from Vibrio alginolyticus

作     者：Yujia ZHANG Shi WANG Jian ZHONG Weijie ZHANG Xing XIAO Zhiqing WEI Huanying PANG Na WANG 

作者机构：College of FisheriesGuangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture/Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education InstitutesZhanjiang 524025China Zhanjiang CustomsZhanjiang 524022China Chinese Academy of Inspection and QuarantineBeijing 100176China 

出 版 物：《Asian Agricultural Research》 (亚洲农业研究（英文）)

年 卷 期：2023年第15卷第7期

页      面：30-34页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean University National Natural Science Foundationof China(32073015) Natural Science Foundation of Guangdong Province(2021A1515011078) Undergraduate Innovation and Entrepreneurship Train-ing Program of Guangdong Ocean University(CXXL2022005) UndergraduateInnovation Team of Guangdong Ocean University(CCTD201802) 

主　　题：Vibrio alginolyticus Gene cloning ndk Bioinformatics analysis 

摘      要：[Objectives]The paper was to clone and analyze bioinformatics of ndk gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the ndk gene sequence of *** *** full length of ndk gene was amplified by PCR and bioinformatics analysis was *** 5.0 software was used to construct NDK phylogenetic tree by neighbor-joining ***-MODEL program was used to obtain the three-dimensional structural model of single subunit from NDK protein.[Results]The ndk gene,molecular structural formula C702H1094N192O214S7,was 426 bp in total,encoding 141 amino acids,with the theoretical molecular weight of 15.87199 kD and the theoretical pI value of *** prediction results of protein subcellular localization,SignalP 5.0,TMHMM Server 2.0 and SoftBerry-Psite showed that NDK mainly existed in the cytoplasm,and the protein was unstable and *** was neither signal peptide cleavage site,nor transmembrane region and KEGG metabolic *** amino acid sequence had two protein kinase C phosphorylation sites,a casein kinaseⅡphosphorylation site,a N-myristoylation site,three microbody C-terminal target signal sites,and a nucleoside diphosphate kinase active *** analysis showed that the NDK of *** had high homology with that of ***,with a similarity of 98.58%.Analysis of the structural functional domain revealed that the protein had one NDK structural functional *** prediction results of secondary structure showed that theα-helix,random coil,β-sheet and extended strand accounted for 53.19%,28.37%,7.09%and 11.35%,*** of NDK protein via STRING database demonstrated that the proteins interacting with NDK protein were NrdA,NrdB,GmK,CmK,TmK,PyrG,PyrH,RelA,FolE and SpoT.[Conclusions]The study plays a positive role in the prevention and control of vibriosis and the improvement of the current aquaculture environment.