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Pedosphere 2024年第2期34卷 424-437页

作者： Wanlin LI Yan XIAO college of Agro-grassland Science Nanjing Agricultural UniversityNanjing 210095China

Microplastic pollution is a global and ubiquitous environmental problem in the oceans as well as in the terrestrial *** examined the fate of microplastic polystyrene(MPS)beads in experimental soil in the presence and absence of symbiotic arbuscular mycorrhizal fungi(AMF)and simulated acid rain(SAR)to determine whether the combinations of these three factors altered the growth of white clover Trifolium *** found that MPS,SAR,or AMF added singly to soil did not alter *** growth or *** contrast,MPS and AMF together significantly reduced shoot biomass,while SAR and MPS together significantly reduced soil available phosphorus independent of AMF *** polystyrene,AMF,and SAR together significantly reduced soil NO_(3)^(-)-*** mycorrhizal fungi added singly also enriched the beneficial soil bacteria(genus Solirubrobacter),while MPS combined with AMF significantly enriched the potential plant pathogenic fungus *** mycorrhizal fungi inoculation with MPS increased the abundance of soil hydrocarbon degraders independent of the presence of *** addition,the abundance of soil nitrate reducers was increased by MPS,especially in the presence of AMF and ***,SAR alone increased the abundance of soil pathogens within the fungal community including antibiotic *** findings indicate that the coexistence of MPS,SAR,and AMF may exacerbate the adverse effects of MPS on soil and plant health.

关键词： acid deposition arbuscular mycorrhizal fungi symbiosis hydrocarbon degradation microbial alpha diversity microplastic pollution pathogen

Historical tillage promotes grass-legume mixtures establishment and accelerates soil microbial activity and organic carbon decomposition

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Journal of Arid Land 2024年第7期16卷 910-924页

作者： ZHOU Jiqiong GONG Jinchao WANG Pengsen SU Yingying LI Xuxu LI Xiangjun LIU Lin BAI Yanfu MA Congyu WANG Wen HUANG Ting YAN Yanhong ZHANG Xinquan Department of grassland Science College of Grassland Science&TechnologySichuan Agricultural UniversityChengdu 611130China Yunnan Sheep Breeding and Extension Center Kunming 655204China

Perennial grass-legume mixtures have been extensively used to restore degraded grasslands,increasing grassland productivity and forage *** is crucial for seedbed preparation and sustainable weed management for the establishment of grass-legume ***,a common concern is that intensive tillage may alter soil characteristics,leading to losses in soil organic carbon(SOC).We investigated the plant community composition,SOC,soil microbial biomass carbon(MBC),soil enzyme activities,and soil properties in long-term perennial grass-legume mixtures under two different tillage intensities(once and twice)as well as in a fenced grassland(FG).The establishment of grass-legume mixtures increased plant species diversity and plant community coverage,compared with *** with once tilled grassland(OTG),twice tilled grassland(TTG)enhanced the coverage of high-quality leguminous forage species by 380.3%.Grass-legume mixtures with historical tillage decreased SOC and dissolved organic carbon(DOC)concentrations,whereas soil MBC concentrations in OTG and TTG increased by 16.0%and 16.4%,respectively,compared with *** significantly decreased the activity of N-acetyl-β-D-glucosaminidase(NAG)by 72.3%,whereas soil enzymeβ-glucosidase(βG)in OTG and TTG increased by 55.9%and 27.3%,respectively,compared with *** analysis indicated a close association of the increase in MBC andβG activities with the rapid decline in *** result suggested that MBC was a key driving factor in soil carbon storage dynamics,potentially accelerating soil carbon cycling and facilitating biogeochemical *** establishment of grass-legume mixtures effectively improves forage quality and boosts plant diversity,thereby facilitating the restoration of degraded *** tillage assists in establishing legume-grass mixtures by controlling weeds,it accelerates microbial activity and organic carbon *** findings provide a foundation for understanding the

关键词： tillage grass-legume mixtures fencing grassland microbial biomass carbon β-glucosidase(βG) N-acetyl-β-D-glucosaminidase(NAG)

Phylotranscriptomic discordance is best explained by incomplete lineage sorting within Allium subgenus Cyathophora and thus hemiplasy accounts for interspecific trait transition

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Plant Diversity 2024年第1期46卷 28-38页

作者： Zengzhu Zhang Gang Liu Minjie Li State Key Laboratory of Herbage Improvement and grassland Agro-Ecosystems College of EcologyLanzhou UniversityLanzhou 730000GansuPR China

The transition of traits between genetically related lineages is a fascinating topic that provides clues to understanding the drivers of speciation and *** can be learned about this process from phylogeny-based trait ***,such inference is often plagued by genome-wide gene-tree discordance(GTD),mostly due to incomplete lineage sorting(ILS)and/or introgressive hybridization,especially when the genes underlying the traits appear ***,by collecting transcriptomes,whole chloroplast genomes(cpDNA),and population genetic datasets,we used the coalescent model to turn GTD into a source of information for ILS and employed hemiplasy to explain specific cases of apparent“phylogenetic discordance”between different morphological traits and probable species phylogeny in the Allium *** concatenation and coalescence methods consistently showed the same phylogenetic topology for species tree inference based on single-copy genes(SCGs),as supported by the KS ***,GTD was high across the genomes of ***:~27%e38.9%of the SCG trees were in conflict with the species *** and nuclear incongruence was also *** coalescent simulations indicated that such GTD was mainly a product of *** hemiplasy risk factor calculations supported that random fixation of ancient polymorphisms in different populations during successive speciation events along the *** phylogeny may have caused the character transition,as well as the anomalous cpDNA *** study exemplifies how phylogenetic noise can be transformed into evolutionary information for understanding character state transitions along species phylogenies.

关键词： Hemiplasy Multispecies coalescence Lineage sorting Gene tree discordance Phylotranscriptomics Allium subg.Cyathophora

The Combination of Achnatherum inebrians Extracts and Soil Microorganisms Inhibited Seed Germination and Seedling Growth in Elymus nutans

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 567-580页

作者： Rui Zhang Taixiang Chen Zhenjiang Chen Hao Chen Xuekai Wei Malik Kamran Chunjie Li State Key Laboratory of Herbage Improvement and grassland Agro-Ecosystems Key Laboratory of Animal Husbandry InnovationMinistry of Agriculture and Rural AffairsEngineering Research Center of Grassland AgricultureMinistry of EducationWest Gansu Province Prataculture Technology Innovation CenterCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou730020China Center for grassland Research National Forestry and Grassland AdministrationCaFBeijing100091China

In a greenhouse experiment,the effects of soil microorganisms and extracts of Achnatherum inebrians on the seed germination and seedling growth of Elymus nutans were *** results showed that both the extracts from aboveground and belowground parts of *** signiﬁcantly inhibited the germination rate,germination potential,germination index,vigor index,seedling height,root length,and fresh weight of ***,but increased malondialdehyde content,catalase,peroxidase and superoxide dismutase activity of *** seedlings(p<0.05).The allelopathy of aqueous extracts of the aboveground parts of *** was stronger than that of the *** extracts of the aboveground parts of *** decreased seed germination rate,germination potential,germination index,vigor index,seedling length,root length,and seedling fresh weight by 10.45%-74.63%,24.18%-32.50%,19.03%-73.36%,37.83%-88.41%,21.42%-53.14%,2.65%-40.21%,and 20.45%-61.36%,respectively,and malondialdehyde content,peroxidase,catalase,and superoxide dismutase activity increased by 8.09%-62.24%,27.83%-86.47%,22.90%-93.17%,and 11.15%-75.91%,*** above indexes were higher in live soil than in sterilized *** microorganisms increased the allelopathy of *** seed germination rate,germination potential,germination index,vigor index,seedling length,and seedling fresh weight of *** planted in live soil decreased by 8.22%-48.48%,10.00%-51.85%,8.19%-53.26%,16.43%-60.03%,12.91%-28.81%,and 9.09%-22.86%compared with sterilized soil,*** content,peroxidase,catalase,and superoxide dismutase activity of *** planted in live soil increased by 53.91%-81.06%,15.71%-57.34%,33.33%-86.31%,and 9.78%-52.51%compared with sterilized soil,*** existence of soil microorganisms enhanced the allelopathy of the secondary metabolites of ***.A combination of microorganisms and aqueous extracts from the aboveground parts of *** had the s

关键词： Achnatherum inebrians water immersion liquid aqueous leachate precipitate allelopathy Elymus nutans soil microorganisms

Cadaverine and putrescine exposure influence carbon and nitrogen cycling genes in water and sediment of the Yellow River

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Journal of Environmental Sciences 2024年第8期142卷 236-247页

作者： Wanghong Su Qiaoling Yu Jiawei Yang Qian Han Sijie Wang Petr Hedenec Xiaochen Wang Ruijun Wan-Yan Huan Li School of Public Health Lanzhou UniversityLanzhou 730000China 2 Institute for Tropical Biodiversity and Sustainable DevelopmentUniversity Malaysia Terengganu21030Kuala NerusTerengganuMalaysia State Key Laboratory of grassland Agro-ecosystems Center for Grassland MicrobiomeCollege of pastoral agriculture science and technology Lanzhou UniversityLanzhou 730000China

The response patterns of microbial functional genes involved in biogeochemical cycles to cadaver decay is a central topic of recent environmental ***,the response mechanisms and pathways of the functional genes associated with the carbon(C)and nitrogen(N)cycling to cadaveric substances such as cadaverine and putrescine remain *** study explored the variation of functional genes associated with C fixation,C degradation and N cycling and their influencing factors under cadaverine,putrescine and mixed *** results showed only putrescine significantly increased the alpha diversity of C fixation genes,while reducing the alpha diversity of N cycling genes in *** the C cycling,the mixed treatment significantly decreased the total abundance of reductive acetyl-CoA pathway genes(i.e.,acsB and acsE)and lig gene linked to lignin degradation in water,while only significantly increasing the hydroxypropionate-hydroxybutylate cycle(i.e.,accA)gene abundance in *** the N cycling,mixed treatment significantly decreased the abundance of the nitrification(i.e.,amoB),denitrification(i.e.,nirS3)genes in water and the assimilation pathway gene(i.e.,gdhA)in *** factors(i.e.,total carbon and total nitrogen)were all negatively associated with the genes of C and N ***,cadaverine and putrescine exposure may inhibit the pathway in C fixation and N cycling,while promoting C *** findings can offer some new insight for the management of amine pollution caused by animal cadavers.

关键词： Carbon cycling Nitrogen cycling Cadaverine Putrescine Pollution management

Effects of formal credit on pastoral household expense: Evidence from the Qinghai-Xizang Plateau of China

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Journal of Integrative Agriculture 2024年第5期23卷 1774-1785页

作者： Yan Zhang Yi Huang Fan Zhang Zeng Tang State Key Laboratory of Herbage Improvement and grassland Agro-ecosystems College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730020China

Formal credit is critical in agricultural production,allowing more expenditure and productive input,thereby improving farmers'*** pastoral China,formal financial institutions are gradually ***,a limited understanding remains of how formal credit affects herders'household *** on a survey of 544 herders from the Qinghai-Xizang Plateau of China,this study adopted the propensity score matching approach to identify the effect of formal credit on herders'total household expenses,daily expenses,and productive *** results found that average age,grassland mortgage,and other variables significantly affected herders'participation in formal *** credit could significantly improve household expenses,especially productive expenses.A heterogeneity analysis showed that formal credit had a greater impact on the household total expense for those at higher levels of wealth;however,it significantly affected the productive expense of herders at lower wealth ***,the mediating effect indicated that formal credit could affect herders'household income,thus influencing their household ***,this study suggests that policies should improve herders'accessibility to formal credit.

关键词： formal credit herders expense Qinghai-Xizang Plateau

Whole-genome resequencing of Hu sheep identifies candidate genes associated with agronomic traits

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Journal of Genetics and Genomics 2024年第8期51卷 866-876页

作者： Liming Zhao Lvfeng Yuan Fadi Li Xiaoxue Zhang Huibin Tian Zongwu Ma Deyin Zhang Yukun Zhang Yuan Zhao Kai Huang Xiaolong Li Jiangbo Cheng Dan Xu Xiaobin Yang Kunchao Han Xiuxiu Weng Weimin Wang State Key Laboratory of Herbage Improvement and grassland Agro-Ecosystems Key Laboratory of Grassland Livestock Industry InnovationMinistry of Agriculture and Rural AffairsEngineering Research Center of Grassland IndustryMinistry of EducationCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhouGansu 730020China Lanzhou Veterinary Research Institute Chinese Academy of Agricultural SciencesLanzhouGansu 730046China college of Animal Science and Technology Gansu Agricultural UniversityLanzhouGansu 730070China

The phenotypic diversity resulting from artificial or natural selection of sheep has made a significant contribution to human *** sheep are a local sheep breed unique to China with high reproductive rates and rapid *** selection signatures have been widely used to investigate the genetic mechanisms underlying phenotypic variation in ***,we conduct whole-genome sequencing of 207 Hu sheep and compare them with the wild ancestors of domestic sheep(Asiatic mouflon)to investigate the genetic characteristics and selection signatures of Hu *** on six signatures of selection approaches,we detect genomic regions containing genes related to reproduction(BMPR1B,BMP2,PGFS,CYP19,CAMK4,GGT5,and GNAQ),vision(ALDH1A2,SAG,and PDE6B),nervous system(NAV1),and immune response(GPR35,SH2B2,PIK3R3,and HRAS).Association analysis with a population of 1299 Hu sheep reveals that those missense mutations in the GPR35(GPR35 g.952651 A>G;GPR35 g.952496 C>T)and NAV1(NAV1 g.84216190 C>T;NAV1 g.84227412 G>A)genes are significantly associated(P<0.05)with immune and growth traits in Hu sheep,*** research offers unique insights into the selection characteristics of Hu sheep and facilitates further genetic improvement and molecular investigations.

关键词： Hu sheep Whole-genome resequencing Selection signature GPR35 NAV1 Single nucleotide polymorphism

A temporal framework for building up of healthy soils

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Frontiers of Agricultural Science and Engineering 2024年第2期11卷 292-296页

作者： Junling ZHANG Jiangzhou ZHANG Yunlong ZHANG Guangzhou WANG State Key Laboratory of Nutrient Use and Management College of Resources and Environmental SciencesNational Academy of Agriculture Green DevelopmentChina Agricultural UniversityBeijing 100193China Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources College of GrasslandResources and EnvironmentInner Mongolia Agricultural UniversityHohhot 010018China Key Laboratory of grassland Management and Utilization Ministry of Agriculture and Rural Affairs of the People’s Republic of ChinaCollege of Grassland Science and TechnologyChina Agricultural UniversityBeijing 100193China

Healthy soils are vital for the diverse services provided by ecosystems to human society~[1], such as provisioning(food,fiber, timber, and fuel), regulation(climate, disease, and natural hazards), waste treatment, nutrient cycling, and cultural services. Soil has a three phase system and the interactions between soil chemical, biological, and physical attributes determine functions(hydraulic, thermal, and gas fluxes).

关键词： soils soil cycling

The Identification of Phenylalanine Ammonia-Lyase(PAL)Genes from Pinus yunnanensis and an Analysis of Enzyme Activity in vitro

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 503-516页

作者： Dejin Mu Lin Chen Heze Wang Zhaoliu Hu Sihui Chen Shi Chen Nianhui Cai Yulan Xu Junrong Tang Key Laboratory of National Forestry and grassland Administration on Biodiversity Conservation in Southwest China Southwest Forestry UniversityKunming650224China college of Forestry Southwest Forestry UniversityKunming650224China

Phenylalanine ammonia lyase(PAL)is the rate-limiting and pivotal enzyme of the general phenylpropanoid path-way,but few reports have been found on PAL genes in Pinus *** the present study,three PAL genes were cloned and identiﬁed from *** seedlings for theﬁrst time,namely,PyPAL-1,PyPAL-2,and *** results indicated that the open-reading frames of PyPAL genes were 2184,2157,and 2385 *** tree analysis revealed that PyPALs have high homology with other known PAL genes in other *** vitro enzymatic analysis showed that all three PyPAL recombinant proteins could catalyze the deamination of L-phenylalanine to form trans-cinnamic acid,but only PAL1 and PAL2 can catalyze the conversion of L-tyrosine toρ-coumaric *** PyPAL genes were expressed in different tissues in 1-year-old ***,and such genes had different expression *** study lays a foundation for further understanding of the biosynthesis of secondary metabolites in ***.

关键词： Pinus yunnanensis phenylalanine ammonia-lyase enzyme activity in vitro functional analysis secondary metabolites

Combining field data and modeling to better understand maize growth response to phosphorus(P) fertilizer application and soil P dynamics in calcareous soils

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Journal of Integrative Agriculture 2024年第3期23卷 1006-1021页

作者： Weina Zhang Zhigan Zhao Di He Junhe Liu Haigang Li Enli Wang School of Biological and Food Processing Engineering Huanghuai UniversityZhumadian 463000China Key Laboratory of Plant-Soil Interactions Ministry of Education/College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources/Key Laboratory of grassland Resource(IMAU) Ministry of Education/College of GrasslandResources and EnvironmentInner Mongolia Agricultural UniversityHohhot 010018China CSIRO Agriculture and Food CanberraACT 2601Australia

We used field experimental data to evaluate the ability of the agricultural production system model (APSIM) to simulate soil P availability,maize biomass and grain yield in response to P fertilizer applications on a fluvo-aquic soil in the North China *** and soil data from a 2-year experiment with three P fertilizer application rates(0,75 and 300 kg P_(2)O_(5) ha^(–1)) were used to calibrate the *** analysis was carried out to investigate the influence of APSIM SoilP parameters on the simulated P availability in soil and maize *** and soil P parameters were then derived by matching or relating the simulation results to observed crop biomass,yield,P uptake and Olsen-P in *** re-parameterized model was further validated against 2 years of independent data at the same *** re-parameterized model enabled good simulation of the maize leaf area index (LAI),biomass,grain yield,P uptake,and grain P content in response to different levels of P additions against both the calibration and validation *** results showed that APSIM needs to be re-parameterized for simulation of maize LAI dynamics through modification of leaf size curve and a reduction in the rate of leaf senescence for modern staygreen maize cultivars in *** P concentration limits (maximum and minimum P concentrations in organs)at different stages also need to be *** results further showed a curvilinear relationship between the measured Olsen-P concentration and simulated labile P content,which could facilitate the initialization of APSIM P pools in the NCP with Olsen-P measurements in future *** remains difficult to parameterize the APSIM SoilP module due to the conceptual nature of the pools and simplified conceptualization of key P transformation processes.A fundamental understanding still needs to be developed for modelling and predicting the fate of applied P fertilizers in soils with contrasting physical and chemical characteristics.

关键词： maize phosphorus availability modeling APSIM maize APSIM SoilP