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Plasma science and technology 2007年第5期9卷 575-577页

作者： Htwe Htwe YIN 黄环唐世彪马庆力 department of Modern Physics University of Science and Technology of ChinaHefei 230026China Center of Physical Experiment and Teaching School of ScienceUniversity of Science and Technology of ChinaHefei 230026China Electronics department Electronic Engineering InstituteHefei 230038China

Positron annihilation lifetime spectroscopy （PALS） is a powerful technique for the study of free volume in polymers. The lifetime of ortho-positronium （o-Ps）, a bound state of an electron and a positron, can be used to assess the pore size, while the intensity can be used to characterize the number of pores. Based on the values of the long-lived o-Ps components in the lifetime spectra, the radii and fractional free volumes of sulfonated poly （2,6-dimethyl-1,4- phenyleneoxide）（SPPO） membranes with added LiCl and SPPO-PES （Polyethersulfone） blend were compared. Free volume radii in both kinds of membranes are discussed.

关键词： positron annihilation free volume pore size SPPO polymer blend

Towards magnetism in pigeon MagR: Iron- and iron-sulfur binding work indispensably and synergistically

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Zoological Research 2023年第1期44卷 142-152页

作者： Yajie Zhou Tianyang Tong Mengke Wei Peng Zhang Fan Fei Xiujuan Zhou Zhen Guo Jing Zhang Huangtao Xu Lei Zhang Shun Wang Junfeng Wang Tiantian Cai Xin Zhang Can Xie Institutes of Physical science and Information technology Anhui UniversityHefeiAnhui 230039China High Magnetic Field Laboratory Hefei Institutes of Physical ScienceChinese Academy of SciencesScience IslandHefeiAnhui 230031China department of Anatomy Anhui Medical UniversityHefeiAnhui 230032China science Island Branch of graduate School University of Science and Technology of ChinaHefeiAnhui 230036China School of Life sciences Peking UniversityBeijing 100871China International Magnetobiology Frontier Research Center Science IslandHefeiAnhui 230031China department of biological Chemistry and Molecular Pharmacology Harvard Medical School250 Longwood AvenueBostonMA 02115USA

The ability to navigate long distances is essential for many animals to locate shelter,food,and breeding *** sense has evolved in various migratory and homing species to orient them based on the geomagnetic field.A highly conserved ironsulfur cluster assembly protein IscA is proposed as an animal magnetoreceptor(MagR).Iron-sulfur cluster binding is also suggested to play an essential role in MagR magnetism and is thus critical in animal *** the current study,we provide evidence for distinct iron binding and iron-sulfur cluster binding in MagR in pigeons,an avian species that relies on the geomagnetic field for navigation and *** MagR showed significantly higher total iron content from both iron-and ironsulfur binding.Y65 in pigeon MagR was shown to directly mediate mononuclear iron binding,and its mutation abolished iron-binding capacity of the ***,both iron binding and iron-sulfur binding demonstrated synergistic effects,and thus appear to be integral and indispensable to pigeon MagR *** results not only extend our current understanding of the origin and complexity of MagR magnetism,but also imply a possible molecular explanation for the huge diversity in animal magnetoreception.

关键词： Animal magnetoreception Iron-sulfur cluster binding Iron binding Magnetism Magnetoreceptor(MagR)

Optically-generated focused ultrasound for noninvasive brain stimulation with ultrahigh precision

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Light(science & Applications) 2022年第11期11卷 2817-2831页

作者： Yueming Li Ying Jiang Lu Lan Xiaowei Ge Ran Cheng Yuewei Zhan Guo Chen Linli Shi Runyu Wang Nan Zheng Chen Yang Ji-Xin Cheng department of Mechanical Engineering Boston UniversityBostonMA 02215USA graduate Program for Neuroscience Boston UniversityBostonMA 02215USA department of Electrical and Computer Engineering Boston UniversityBostonMA 02215USA department of Chemistry Boston UniversitBostonMA 02215USA department of Biomedical Engineering Boston UniversityBostonMA 02215USA Division of Materials science and Engineering Boston UniversityBostonMA 02215USA department of biological Engineering Massachusetts Institute of TechnologyCambridgeMA 02142USA

High precision neuromodulation is a powerful tool to decipher neurocircuits and treat neurological *** non-invasive neuromodulation methods offer limited precision at the milimeter ***,we report opticallygenerated focused ultrasound(OFUS)for non-invasive brain stimulation with ultrahigh *** is generated by a soft optoacoustic pad(SOAP)fabricated through embedding candle soot nanoparticles in a curved polydimethylsiloxane *** generates a transcranial ultrasound focus at 15 MHz with an ultrahigh lateral resolution of 83μm,which is two orders of magnitude smaller than that of conventional transcranial-focused ultrasound(tFUS).Here,we show effective OFUS neurostimulation in vitro with a single ultrasound *** demonstrate submillimeter transcranial stimulation of the mouse motor cortex in *** acoustic energy of 0.6 mJ/cm?,four orders of magnitude less than that of tFUS,is suffcient for successful OFUS *** offers new capabilities for neuroscience studies and disease treatments by delivering a focus with ultrahigh precision noninvasively.

关键词： SOAP stimulation precision

Performance of biological phosphorus removal and characteristics of microbial community in the oxic-settling-anaerobic process by FISH analysis

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Journal of Zhejiang University-science A(Applied Physics & Engineering) 2008年第7期9卷 1004-1010页

作者： Jian-fang WANG Qing-liang ZHAO Wen-biao JIN Shi-jie YOU Jin-na ZHANG School of Municipal and Environmental Engineering Harbin Institute of Technology Harbin 150090 China department of Urban and Civil Engineering Harbin Institute of Technology Shenzhen Graduate School Shenzhen 518055 China

Performance of biological phosphorus removal in the oxic-settling-anaerobic(OSA) process was investigated. Cell staining and fluorescent in situ hybridization(FISH) were used to analyze characteristics and microbial community of sludge. Experimental results showed that phosphorus removal efficiency was near 60% and the amount of biological phosphorus accumulation in aerobic sludge of the OSA system was up to 26.9 mg/g. biological phosphorus removal efficiency was partially inhibited by carbon sources in the continuous OSA system. Contrasted to the OSA system,biological phosphorus removal efficiency was enhanced by 14% and the average total phosphorus(TP) contents of aerobic sludge were increased by 0.36 mg/g when sufficient carbon sources were supplied in batch experiments. Staining methods indicated that about 35% of microorganisms had typical characteristics of phosphorus accumulating organisms(PAOs) . FISH analysis demonstrated that PAOMIX-binding bacteria were predominant microbial communities in the OSA system,which accounted for around 28% of total bacteria.

关键词： Excess sludge reduction biological phosphorus removal Phosphate accumulating organisms (PAOs) DAPI(4' 6'-diamidino-2-phenyl indol dihydrochloride) Fluorescent in situ hybridization (FISH)

Microfluidic Device for Simple Diagnosis of Plant Growth Condition by Detecting miRNAs from Filtered Plant Extracts

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Plant Phenomics 2024年第2期6卷 364-376页

作者： Yaichi Kawakatsu Ryo Okada Mitsuo Hara Hiroki Tsutsui Naoki Yanagisawa Tetsuya Higashiyama Akihide Arima Yoshinobu Baba Ken-ichi Kurotani Michitaka Notaguchi Bioscience and Biotechnology Center Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan graduate School of Bioagricultural sciences Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan department of Molecular and Macromolecular Chemistry Graduate School of EngineeringNagoya UniversityFuro-choChikusa-kuNagoyaAichi 464-8603Japan graduate School of science Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Institute of Transformative Bio-Molecules Nagoya UniversityNagoya 464-8601Japan department of biological sciences Graduate School of ScienceThe University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japan Institute of Nano-Life-Systems Institutes of Innovation for Future SocietyNagoya UniversityFuro-choChikusa-kuNagoya 464-8603Japan department of Biomolecular Engineering Graduate School of EngineeringNagoya UniversityFuro-choChikusa-kuNagoya 464-8603Japan Institute of Quantum Life science National Institutes for Quantum Science and Technology(QST)Anagawa 4-9-1Inage-kuChiba 263-8555Japan department of Botany Graduate School of ScienceKyoto UniversityKitashirakawa Oiwake-choSakyo-kuKyoto 606-8502Japan

Plants are exposed to a variety of environmental stress,and starvation of inorganic phosphorus can be a major constraint in crop *** plants,in response to phosphate deficiency in soil,miR399,a type of microRNA(miRNA),is *** detecting miR399,the early diagnosis of phosphorus deficiency stress in plants can be ***,general miRNA detection methods require complicated experimental ***,simple and rapid miRNA detection methods are required for early plant nutritional diagnosis.

关键词： mirnas plant device simple growth from condition detecting diagnosis extracts

Robust exponential stabilization of nonholonomic wheeled mobile robots with unknown visual parameters

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控制理论与应用（英文版） 2011年第2期9卷 295-301页

作者： Liang, Zhenying Wang, Chaoli department of Control science and Engineering University of Shanghai for Science and Technology Shanghai 200093 China School of science Shandong University of Technology Zibo Shandong 255049 China

The visual servoing stabilization of nonholonomic mobile robot with unknown camera parameters is investigated.A new kind of uncertain chained model of nonholonomic kinemetic system is obtained based on the visual feedback and the standard chained form of type (1,2) mobile ***,a novel time-varying feedback controller is proposed for exponentially stabilizing the position and orientation of the robot using visual feedback and switching strategy when the camera parameters are not *** exponential stability of the closed-loop system is rigorously *** results demonstrate the effectiveness of the method proposed in this paper.

关键词： Uncertain chained form Nonholonomic system Stabilization Visual feedback

Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation:A preliminary study

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Asian Pacific Journal of Tropical Biomedicine 2016年第7期6卷 568-573页

作者： Md.Moklesur Rahman Sarker Long Chiau Ming Md.Zaidul Islam Sarker M Shahabuddin Kabir Choudhuri department of Immunochemistry Division of Pharmaceutical SciencesOkayama University Graduate School of MedicineDentistry and Pharmaceutical Sciences department of Pharmacology Faculty of PharmacyLincoln University College Faculty of Pharmacy Brain Degeneration and Therapeutics GroupPharmaceutical and Life Sciences Co Re Universiti Teknologi MARA department of Pharmaceutical technology Faculty of PharmacyInternational Islamic University Malaysia department of Pharmacy Faculty of Biological ScienceJahangirnagar University

Objective:To assess the immunopotentiality of Ayurvedic polyherbal preparations,"Saribadi" and "Anantamul Salsa".Methods: Freshly prepared BALB/c mice splenocytes were cultured with "Saribadi" or"Anantamul Salsa" treatment [doses of 0.25%, 0.50%, 0.75%, 1.00%, 1.50%, 2.00%,3.00% and 4.00%(v/v)] at 37C for 5 days. The immunoglobulin M(IgM) production and lymphocytes proliferation were determined by ELISA and MTT methods, *** contamination was assessed by treating the preparations with polymyxin ***: The doses of "Saribadi" [0.25%, 0.50%, 0.75% and 1.00%(v/v)] significantly increased IgM productions(0.966, 0.728, 0.695 and 0.615 mg/m L vs. control 0.265 mg/m L)and lymphocytes proliferation [absorbance 0.311, 0.394, 0.372 and 0.334 optical density(OD) vs. control 0.162 OD]. Similarly, the doses of "Anantamul Salsa" [0.50%, 0.75%,1.00% and 1.50%(v/v)] promoted IgM productions(0.933, 0.919, 0.917 and 0.892 mg/m L vs. control 0.502 mg/m L) and the doses of "Anantamul Salsa" [0.50%, 0.75%, 1.00%,1.50%, 2.00%, and 3.00%(v/v)] stimulated lymphocytes proliferation(absorbance 0.395,0.326, 0.440, 0.398, 0.452 and 0.355 OD vs. control 0.199 OD). The activity of "Saribadi"and "Anantamul Salsa" was not retarded by the treatment of preparations with polymyxin ***: Immunomodulatory activity of "Saribadi" and "Anantamul Salsa" was unveiled for the first time. "Saribadi" and "Anantamul Salsa" possess immunostimulating potential acting through the induction of lymphocyte proliferation and IgM *** preparations may be useful in strengthening immune responses. However, further cellular and in vivo studies are required.

关键词： "Saribadi" "Anantamul Salsa" Ayurvedic medicine Immunostimulant Lymphocytes Splenocytes Immunoglobulin M antibody Proliferation Differentiation

Stress-Induced Flowering in Pharbitis—A Review

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American Journal of Plant sciences 2013年第12期4卷 74-79页

作者： Kaede C. Wada Mizuki Yamada Kiyotoshi Takeno department of Applied biological Chemistry Faculty of Agriculture Niigata University Niigata Japan graduate School of science and technology Niigata University Niigata Japan graduate School of science and technology Niigata University Niigata Japan department of Biology Faculty of Science Niigata University Niigata Japan

Many plant species are induced to flower by stress. Stress-induced flowering has been studied mostly in the short-day plant pharbitis (also called Japanese morning glory;Ipomoea nil, formerly Pharbitis nil). In this article, physiological characteristics, the regulation by salicylic acid (SA) and the expression of flowering-related genes in stress-induced flowering in pharbitis are reviewed. Pharbitis flowered under long-days in response to poor nutrition or low temperature. The pharbitis plants induced to flower by stress reached anthesis, fruited and produced fertile seeds. The progeny of the stressed plants developed normally. Grafting experiments indicated that a transmissible flowering stimulus is involved in poor nutrition stress-induced flowering. Aminooxyacetic acid (AOA), a phenylalanine ammonia-lyase (PAL) inhibitor, inhibited the stress-induced flowering, and this inhibition was overcome by SA. Stress induced PAL activity and SA biosynthesis. PnFT2, a pharbitis ortholog of the flowering gene FLOWERING LOCUS T of Arabidopsis thaliana, was expressed when the plants were induced to flower by stress. The overexpression of PnFT2 induced flowering, and PnFT2RNAi inhibited it. AOA inhibited PnFT2 expression induced by stress, and SA eliminated this inhibitory effect. SA enhanced PnFT2 expression under poor nutrition but not under non-stressful conditions. Therefore, stress may induce the production of SA and other unknown factor(s) that may work in combination to induce PnFT2 expression and flowering.

关键词： Flowering Pharbitis PnFT Salicylic Acid Stress

Estradiol agonists inhibit human Lo Vo colorectal-cancer cell proliferation and migration through p53

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World Journal of Gastroenterology 2014年第44期20卷 16665-16673页

作者： Hsi-Hsien Hsu Wei-Wen Kuo Da-Tong Ju Yu-Lan Yeh Chuan-Chou Tu Ying-Lan Tsai Chia-Yao Shen Sheng-Huang Chang Li-Chin Chung Chih-Yang Huang Division of Colorectal Surgery Mackay Memorial Hospital Mackay Junior College of Medicine Nursing and Management department of biological science and technology China Medical University department of Neurological Surgery Tri-Service General Hospital National Defense Medical Center department of Pathology Changhua Christian Hospital Division of Chest Medicine Department of internal Medicine Armed Force Taichung General Hospital National Taiwan Sport University Athletic Training and Health Department department of Nursing Mei Ho University Tsao-Tun Psychiatric Center Department of Health Executive Yuan department of Hospital and Health Care Administration Chia Nan University of Pharmacy and Science graduate Institute of Chinese Medical science China Medical University graduate Institute of Basic Medical science China Medical University department of Health and Nutrition Biotechnology Asia University

AIM: To investigate the effects of 17β-estradiol via estrogen receptors (ER) or direct administration of ER agonists on human colorectal cancer.

关键词： Estrogen Estrogen agonist Estrogen receptors Human colon cancer cell p53

Rapid species identification of pathogenic bacteria from a minute quantity exploiting three-dimensional quantitative phase imaging and artificial neural network

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Light(science & Applications) 2022年第7期11卷 1595-1606页

作者： Geon Kim Daewoong Ahn Minhee Kang Jinho Park DongHun Ryu YoungJu Jo Jinyeop Song Jea Sung Ryu Gunho Choi Hyun Jung Chung Kyuseok Kim Doo Ryeon Chung In Young Yoo Hee Jae Huh Hyun-seok Min Nam Yong Lee YongKeun Park department of Physics Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea KAIST Institute for Health science and technology KAISTDaejeon 34141Republic of Korea Tomocube Inc. Daejeon 34109Republic of Korea Smart Healthcare&Device Research Center Samsung Medical CenterSungkyunkwan University School of MedicineSeoul 06351Republic of Korea graduate School of Nanoscience and technology Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea department of biological sciences Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea department of Emergency Medicine Bundang CHA HospitalSeongnam-siGyeonggi-Do 13496Korea Division of Infectious Diseases Department of Internal MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoul 06351Republic of Korea department of Laboratory Medicine Seoul St.Mary's HospitalCollege of MedicineThe Catholic University of KoreaSeoul 06591Republic of Korea department of Laboratory Medicine and Genetics Samsung Medical CenterSungkyunkwan University School of MedicineSeoul 06351Republic of Korea department of Applied Physics Stanford UniversityStanfordCA 94305USA department of Physics Massachusetts Institute of TechnologyCambridgeMA 02139USA

The healthcare industry is in dire need of rapid microbial identification techniques for treating microbial *** infections are a major healthcare issue worldwide,as these widespread diseases often develop into deadly *** studies have shown that an early appropriate antibiotic treatment significantly reduces the mortality of an infection,this effective treatment is difficult to *** main obstacle to early appropriate antibiotic treatments is the long turnaround time of the routine microbial identification,which includes time-consuming sample ***,we propose a microscopy-based framework that identifies the pathogen from single to few *** framework obtains and exploits the morphology of the limited sample by incorporating three-dimensional quantitative phase imaging and an artificial neural *** demonstrate the identification of 19 bacterial species that cause bloodstream infections,achieving an accuracy of 82.5%from an individual bacterial cell or *** performance,comparable to that of the gold standard mass spectroscopy under a sufficient amount of sample,underpins the effectiveness of our framework in clinical ***,our accuracy increases with multiple measurements,reaching 99.9%with seven different measurements of cells or *** believe that our framework can serve as a beneficial advisory tool for clinicians during the initial treatment of infections.

关键词： artificial neural exploit