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Characterization of the infectivity of an Indonesian Zika virus strain in mammalian cell lines

Asian Pacific Journal of Tropical Biomedicine 2024年第5期14卷 215-224页

作者： Justus Thomas Obiajulu Sievers Anom Bowolaksono R.Tedjo Sasmono graduate School of Biology Department of BiologyFaculty of Mathematics and Natural SciencesUniversitas IndonesiaDepok 16424Indonesia Eijkman Research Center for Molecular Biology National Research and Innovation AgencyCibinong 16911Indonesia Cellular and Molecular Mechanisms in Biological System Research group Department of BiologyFaculty of Mathematics and Natural SciencesUniversitas IndonesiaDepok 16424Indonesia

Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell ***:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell *** replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using *** cytometry was used to quantify the infected cells and cell viability was measured using an MTT *** ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining ***:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and *** contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell ***:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 *** on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery.

关键词： Zika virus Infectivity Replication Cell lines In vitro

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A Rare Case of Aortic Valve Endocarditis and Acute Meningitis Due to Haemophilus influenzae

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Case Reports in Clinical Medicine 2024年第6期13卷 207-211页

作者： Zachary M. Visinoni Justin D. Tse Christopher F. Pease Department of graduate Medical Education Internal Medicine Residency Sutter Roseville Medical Center Roseville USA Sutter Medical group Roseville USA

HACEK organisms represent a rare but important group of causative pathogens in endocarditis. These bacteria have historically been associated with culture-negative endocarditis;however, modern laboratory techniques have made this less common. In this case, we present a 74-year-old man who presented with acute onset altered mentation, fever, and sepsis. He was ultimately found to have Haemophilus influenzae meningitis, cerebral empyema, aortic valve endocarditis, psoas myositis, and L2 - L3 diskitis with osteomyelitis. Although HACEK organisms are commonly found in the oropharynx and upper respiratory tract in humans, our patient did not report recent preceding dental or ENT procedures. H. influenzae is responsible for approximately 0.16% of all cases of bacterial endocarditis, representing a very limited subset. Although generally considered low virulent pathogens, this case demonstrates the unusual extent of infection from a HACEK organism, H. influenzae, causing aortic valve endocarditis as well as atypical non-cardiac sequelae, including acute meningitis.

关键词： HACEK Haemophilus influenzae Aortic Valve Endocarditis Bacterial Meningitis Cerebral Empyema

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The effect of glutathione on glucosinolate biosynthesis through the sulfur assimilation pathway in pakchoi associated with the growth conditions

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Horticultural Plant Journal 2024年第2期10卷 473-487页

作者： Biao Zhu Zhile Liang Dan Wang Chaochao He Zhujun Zhu Jing Yang Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable Ministry of Agriculture and Rural AffairsCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang ProvinceCollege of Horticulture ScienceZhejiang A&F UniversityHangzhouZhejiang 311300China

Glucosinolates(GSLs) are a group of nitrogen-and sulfur-containing secondary metabolites, synthesized primarily in members of the Brassicaceae family, that play an important role in food flavor, plant antimicrobial activity, resistance to insect attack, stress tolerance, and human anti-cancer effects. As a sulfur-containing compound, glutathione has a strong connection with GSLs biosynthesis as a sulfur donor or redox system, and exists in reduced(glutathione;GSH) and oxidized(glutathione disulfide;GSSG) forms. However, the mechanism of GSH regulating GSLs biosynthesis remainds unclear. Hence, the exogenous therapy to pakchoi under normal growth condition and sulfur deficiency condition were conducted in this work to explore the relevant mechanism. The results showed that exogenous application of buthionine sulfoximine, an inhibitor of GSH synthesis, decreased the transcript levels of GSLs synthesis-related genes and transcription factors, as well as sulfur assimilation-related genes under the normal growth condition. Application of exogenous GSH inhibited the expression of GSLs synthesis-and sulfur assimilation-related genes under the normal condition, while the GSLs biosynthesis and the sulfur assimilation pathway were activated by exogenous application of GSH when the content of GSH in vivo of plants decreased owing to sulfur deficiency. Moreover,exogenous application of GSSG increased the transcript levels of GSLs synthesis-and sulfur assimilation-related genes under the normal growth condition and under sulfur deficiency. The present work provides new insights into the molecular mechanisms of GSLs biosynthesis underlying glutathione regulation.

关键词： Pakchoi Glucosinolates Reduced glutathione Oxidized glutathione Sulfur assimilation

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Single-cell transcriptome atlases of soybean root and mature nodule reveal new regulatory programs that control the nodulation process

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Plant Communications 2024年第8期5卷 69-91页

作者： Sergio Alan Cervantes-Perez Prince Zogli Sahand Amini Sandra Thibivilliers Sutton Tennant Md Sabbir Hossain Hengping Xu Ian Meyer Akash Nooka Pengchong Ma Qiuming Yao Michael J.Naldrett Andrew Farmer Olivier Martin Samik Bhattacharya Jasper Klaver Marc Libault Department of agronomy and horticulture University of Nebraska-LincolnLincolnNE 68503USA Division of Plant Science and Technology College of AgricultureFoodand Natural ResourcesUniversity of Missouri-ColumbiaColumbiaMO 65211USA Interdisciplinary Plant group of Missouri-Columbia ColumbiaMO 65211USA School of Computing University of Nebraska-LincolnLincolnNE 68503USA Proteomics and Metabolomics Facility Center for BiotechnologyUniversity of Nebraska-LincolnLincolnNE 68588USA National Center for Genome Resources Santa FeNM 87505USA INRAE UniversiteParis-SaclayInstitut des Sciences des Plantes de Paris SaclayIPS2Batiment 630 Plateau du MoulonRue Noetzlin91192 Gif sur Yvette CedexFrance Resolve Biosciences Monheim am RheinGermany Present address:The School of Plant Sciences University of ArizonaTucsonAZ 85721USA Present address:GALY Co. 100 Hood Park Dr.BostonMA 02126USA

The soybean root system is *** addition to being composed of various cell types,the soybean root system includes the primary root,the lateral roots,and the nodule,an organ in which mutualistic symbiosis with N-fixing rhizobia occurs.A mature soybean root nodule is characterized by a central infection zone where atmospheric nitrogen is fixed and assimilated by the symbiont,resulting from the close cooperation between the plant cell and the *** date,the transcriptome of individual cells isolated from developing soybean nodules has been established,but the transcriptomic signatures of cells from the mature soybean nodule have not yet been *** single-nucleus RNA-seq and Molecular Cartography technologies,we precisely characterized the transcriptomic signature of soybean root and mature nodule cell types and revealed the co-existence of different sub-populations of ***-infected cells in the mature soybean nodule,including those actively involved in nitrogen fixation and those engaged in *** of the single-cell-resolution nodule transcriptome atlas and the associated gene co-expression network confirmed the role of known nodulation-related genes and identified new genes that control the nodulation *** instance,we functionally characterized the role of GmFWL3,a plasma membrane microdomain-associated protein that controls rhizobial *** study reveals the unique cellular complexity of the mature soybean nodule and helps redefine the concept of cell types when considering the infection zone of the soybean nodule.

关键词： soybean root nodule single-cell RNA-seq nanodomains

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Impact of Outdoor Activities in Different Landscape Environments on the Physical and Mental Health of College Students

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Journal of Landscape Research 2024年第4期16卷 63-65,69页

作者： LIU Yi LIANG Fahui WANG Dan LIU Huiqin WANG Peinan SHI Jiaqi College of horticulture and Landscape Architecture Tianjin Agricultural UniversityTianjin 300392China

To investigate whether outdoor activities have an improvement effect on the emotions of young people and the differences in the impact of outdoor activities on the emotions of young people under different landscape environmental conditions,the participating students are asked to engage in outdoor activities in different landscape environments and divided into four *** group is assigned to engage in outdoor activities in four types of landscape areas:open lawn area,low shrub area,dense forest area,and waterfront *** activities include outdoor yoga and making leaf *** results show that outdoor yoga has the best effect on reducing pulse and blood pressure and regulating emotions in open lawn area and waterfront area,followed by leaf mantras in waterfront area and outdoor yoga in dense forest ***,both activities have no significant effect in low shrub area.

关键词： Outdoor activities College student Emotional regulation Landscape environment

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Study on the Biological Characteristics,Pathogenicity Differentiation and Genetic Diversity of Sclerotinia minor Isolated from Different Hosts

Study on the Biological Characteristics,Pathogenicity Differ...

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中国植物病理学会2024年学术年会

作者： HAO Baizhen ZHANG Jian ZHAO Jun College of horticulture and Plant Protection Inner Mongolia Agricultural University

Sclerotinia is a fungal disease caused by pathogenic fungi of the genus *** this study,we took Sclerotinia minor strains isolated from both sunflower and potato in different regions of Inner Mongolia as the objects of research and studied their biological characteristics,pathogenicity differentiation,and genetic ***,we isolated and purified 12 and 28 strains of *** separately from both potato and sunflower diseased plants,collected from Chahar Right-Wing Qian Banner of Ulaanchab *** isolates were confirmed to be *** by Koch's ***,the biological characteristics and pathogenicity of the above samples were *** results suggested that the growth rate of the mycelium of *** from potatoes was faster than that of *** from sunflower,but mycelium of *** from potatoes grew rather sparse on the PDA plate than that of isolates from *** was no significant difference on oxalic acid(OA) secretion and PG enzyme activity among all tested *** results of cross-infection suggested that *** from potato had a higher pathogenicity on its isolated host potato than that of the *** from sunflower;but there is no difference on the pathogenicity of all tested isolates on ***,Mycelium compatibility group(MCG) of tested isolates of *** from sunflower showed that the 120 isolates were divided into 21 MCG groups,and the isolates collected from the same sunflower field were distributed within different MCG *** isolates from potatoes were also classified into four different MCG *** results of mating types identification suggested that Inv-was the dominant type among all tested *** analysis results indicated that 26 strains of *** from sunflower and 10 strains of *** from potato s were interspersed in different genetic clusters,indicating the dramatic genetic difference of *** isolated from both sunflower and *** summary,this is

关键词： Sclerotinia minor Biological characteristics Genetic diversity Pathogenicity differentiation Genetic transformation

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α,β-Dehydroamino Acid Synthesis through Proline-Catalyzed Aldol Condensation with a Glycine Schiff Base

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Precision Chemistry 2024年第1期2卷 14-20页

作者： Jun Sawamura Daikan Ieiri Ryo Yazaki Takashi Ohshima graduate School of Pharmaceutical Sciences Kyushu UniversityFukuoka 812-8582Japan

A general protocol for the synthesis ofα,β-dehydroamino acids and their peptides was *** efficiently catalyzed an aldol condensation reaction of a glycine Schiff base with a variety of *** hydroxy group on the benzophenone imine was crucial for high Z/E selectivity and further transimination for protecting group-freeα,β-dehydroamino *** elongation of both the C-and N-terminals highlighted the usefulness of our present protocol.

关键词： α β-dehydroamino acid peptide proline aldol condensation glycine Schiff base transimination

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Effects of biofertilizer and water-saving irrigation interactions on the leaf photosynthesis and plant growth of tomatoes

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International Journal of Agricultural and Biological Engineering 2024年第2期17卷 177-185页

作者： Danyan Chen Yuchun Ai Peizhe Li Yue Dong Hailing Li Changyi Wang Xiaorui Jiang Shilong Li Yan Shen Aijun Dai Yuanyuan Feng College of horticulture Jinling Institute of TechnologyNanjing 210038China Co-Innovation Center for Sustainable Forestry in Southern China College of Forestry and GrasslandCollege of Soil and Water ConservationNanjing Forestry UniversityNanjing 210037China Institute of Agricultural Resources and Environment Jiangsu Academy of Agricultural SciencesNanjing 210014China Agriculture Exchange and Cooperation Center of Shandong Province Jinan 250100China Murdoch Applied Innovation Nanotechnology Research group College of ScienceHealthEngineering and EducationMurdoch UniversityPerthWA 5150Australia

Irrigation and fertilizer interaction is an efficient cultivation management strategy for facility ***,the effects of irrigation and fertilizer management on tomato growth and its physiological factors remain *** this study,two irrigation patterns(W1,conventional irrigation;W2,water-saving irrigation)and four fertilizer application patterns(CF,chemical fertilizer;BOF,biological organic fertilizer;NPK,nutrient compound fertilizer;BOF+NPK)were selected to observe the effects of their interaction on cherry tomato plant growth,leaf photosynthesis and fruit quality through pot *** results showed that W2 treatments promoted plant height growth compared to W1 under the same fertilizer ***,irrigation and fertilizer management had significant effects on net photosynthetic rate,intercellular oxidation concentration,stomatal conductance and transpiration rate at the first sequence flowering and fruiting *** maximum tomato plant height(99.0 cm)was achieved under the irrigation and fertilizer pattern of BOF and W2,along with the highest fruit yield of 1.98 kg/plant,which was approximately 31.1%higher than the minimum yield under the combined CF and W2 *** W2 treatments,the application of either NPK or BOF increased the soluble sugar content of *** structural equation models showed that the soil alkali hydrolyzed nitrogen could directly significantly affect the yield and soluble *** findings suggest that optimization of irrigation-fertilizer interactions positively regulates tomato growth,providing an efficient model for tomato irrigation and fertilizer management and a reference for sustainable development of facility agriculture.

关键词： irrigation-fertilization interaction cherry tomato plant height leaf photosynthesis fruit yield and quality

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Revealing the intrinsic mechanisms for accelerating nitrogen removal efficiency in the Anammox reactor by adding Fe(Ⅱ)at low temperature

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Chinese Chemical Letters 2024年第8期35卷 341-348页

作者： Linjing Li Wenlai Xu Jianyong Ning Yaping Zhong Chuyue Zhang Jiane Zuo Zhicheng Pan State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of TechnologyChengdu 610059China State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China National Postdoctoral Research Station Haitian Water GroupChengdu 610213China National Enterprise Technology Center Haitian Water GroupChengdu 610213China Water Safety and Water Pollution Control Engineering Technology Research Center in Sichuan Province Chengdu 610213China Laboratory of Municipal Wastewater Treatment Technology in Sichuan Province Chengdu 610213China Institute of Environment and Ecology Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China

Fe(Ⅱ)is an essential trace element for anaerobic ammonium oxidation bacteria(AAOB)metabolism,and can improve the nitrogen removal efficiency of anaerobic ammonia oxidation(Anammox).Here we oper-ated two identical expanded granular sludge bed(EGSB)reactors at low temperature(15±3℃)for 154 *** 1(R_(1))received additional Fe(Ⅱ)(0.12 mmol/L)during the late startup phase,while reactor 0(R_(0))served as the control and did not receive extra Fe(Ⅱ).Nitrogen removal in R_(1)became stable at 55 d of operation,ten days earlier than R_(0).The nitrogen removal rate(NRR)of R_(1) was 1.64 kg N m^(−3)d^(−1)and its TN removal rate was as high as 89%,while R_(0)only reached 75%.The addition of Fe(Ⅱ)was fur-ther beneficial to aggregation and stability of the granular sludge,and the used sludge of both reactors showed enrichment for AAOB populations compared to the inoculum,for instance,increased abundance of Candidatus-Kuenenia and in particular of Candidatus-Brocadia(from 0.17%to 10.10%in R_(0)and 7.79%in R_(1)).Diverse microbial species and complex microbial network structure in R_(1)compared to R_(0)promoted the coupled denitrogenation by Anammox,dissimilatory nitrate reduction to ammonium(DNRA),nitrate-dependent Fe oxidation(NDFO),and ferric ammonium oxidation(Feammox).In addition,the microbial community in R_(1)was more resistant to short-term low temperature(2-7℃)starvation,illustrating a further positive effect of adding Fe(Ⅱ)during the startup phase of an Anammox reactor.

关键词： Ambient low temperature Anammox Fe(Ⅱ) Extracellular polymeric substances Microbial community Microbial network analysis

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Xuebijing improves intestinal microcirculation dysfunction in septic rats by regulating the VEGF-A/PI3K/Akt signaling pathway

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World Journal of Emergency Medicine 2024年第3期15卷 206-213页

作者： A-ling Tang Yan Li Li-chao Sun Xiao-yu Liu Nan Gao Sheng-tao Yan Guo-qiang Zhang Department of Emergency China-Japan Friendship HospitalBeijing 100029China graduate School Beijing University of Chinese MedicineBeijing 100105China

BACKGROUND:This study aims to explore whether Xuebijing(XBJ) can improve intestinal microcirculation dysfunction in sepsis and its ***:A rat model of sepsis was established by cecal ligation and puncture(CLP).A total of 30 male SD rats were divided into four groups:sham group,CLP group,XBJ + axitinib group,and XBJ *** was intraperitoneally injected 2 h before *** data(blood pressure and heart rate) were *** intestinal microcirculation data of the rats were analyzed via microcirculation ***-linked immunosorbent assay(ELISA) kits were used to detect the serum levels of interleukin-6(IL-6),C-reactive protein(CRP),and tumor necrosis factor-α(TNF-α) in the *** analysis and transmission electron microscopy were used to analyze the injury of small intestinal microvascular endothelial cells and small intestinal mucosa in *** expression of vascular endothelial growth factor A(VEGF-A),phosphoinositide 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(Akt),and phosphorylated Akt(p-Akt) in the small intestine was analyzed via Western ***:XBJ improved intestinal microcirculation dysfunction in septic rats,alleviated the injury of small intestinal microvascular endothelial cells and small intestinal mucosa,and reduced the systemic inflammatory ***,XBJ upregulated the expression of VEGF-A,p-PI3K/total PI3K,and p-Akt/total Akt in the rat small ***:XBJ may improve intestinal microcirculation dysfunction in septic rats possibly through the VEGF-A/PI3K/Akt signaling pathway.

关键词： Sepsis Xuebijing Vascular endothelial growth factor A Microcirculation Rat Phosphoinositide 3-kinase Protein kinase B

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