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Unraveling betalain suppression in pitaya:insights from co-activator HuMYB9 binding at HuCYP76AD1-1,HuADH1,and HuDODA1 super-enhancers

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Food Quality and Safety 2024年第2期8卷 404-413页

作者： Fangfang Xie Kamran Shah Canbin Chen Irfan Ali Sabir Jiayi Chen Jiaxuan Chen Jianye Chen Yonghua Qin Guangdong Provincial Key laboratory of Postharvest Science of fruits and vegetables/Key laboratory of Biology and genetic improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsCollege of HorticultureSouth China Agricultural UniversityGuangzhouChina Department of Horticulture College of AgricultureGuangxi UniversityNanningChina

Objectives:Myeloblastosis(MYB)proteins,recognized as crucial transcriptional regulators,play an integral role in orchestrating the range of plant *** and Methods:Pitaya(Hylocereus spp.),a distinctive commercially cultivated fruit,stands apart due to its unique betalains,water-soluble nitrogen-enriched pigments that confer its vibrant *** betalain biosynthesis has been extensively explored,the potential infuence of co-activators that might disrupt pigment production remains relatively ***:In this study,we found that HuMYB9,an R2R3-MYB repressor,exhibited a remarkable decreasing during pitaya *** alignment analyses showed conserved R2 and R3 domains within the C-terminal of ***9’s regulatory activities were found to be nuclear localized and it interacted with specifc elements within the promoters of HuDODA1,HuADH1,and HuCYP76AD1-1,thus infuencing the transcriptional activities in ***9 transiently downregulated the expression of key betalain biosynthetic genes with a corresponding effect on the levels of pitaya pulp ***:Our results suggest that HuMYB9 operates as a suppressor,specifcally downregulating the expression of HuCYP76AD1-1,HuDODA1,and HuADH,thereby modulating betalain biosynthesis in ***,our fndings provide invaluable insights into the regulation of betalain accumulation in pitaya.

关键词： Betalains color pigments cactus MYB transcription factor pitaya

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The WRKY transcription factor HpWRKY44 regulates CytP450-like1 expression in red pitaya fruit(Hylocereus polyrhizus)

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Horticulture Research 2017年第1期4卷 192-200页

作者： Mei-nv Cheng Zi-juan Huang Qing-zhu Hua Wei Shan Jian-fei Kuang Wang-jin Lu Yong-hua Qin Jian-ye Chen State Key laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key laboratory of Postharvest Science of fruits and vegetables/Key laboratory of Biology and genetic improvement of Horticultural Crops-South China Ministry of AgricultureCollege of HorticultureSouth China Agricultural UniversityGuangzhou510642China

Red pitaya(Hylocereus polyrhizus)fruit is a high-value,functional food,containing a high level of *** genes potentially related to betalain biosynthesis,such as cytochrome P450-like(CytP450-like),have been identified in pitaya fruit,while their transcriptional regulation remains *** this work,the potential involvement of a WRKY transcription factor,HpWRKY44,in regulating CytP450-like1 expression in pitaya fruit was ***44,a member of the Group 1 WRKY family,contains two conserved WRKY motifs and is localized in the ***44 also exhibits trans-activation *** expression analysis showed that the expression of HpCytP450-like1 and HpWRKY44 increased steadily during pitaya fruit coloration,which corresponded with the production of elevated betalain levels in the ***44 was also demonstrated to directly bind to and activate the HpCytP450-like1 promoter via the recognition of the W-box element present in the ***,our findings indicate that HpWRKY44 transcriptionally activates HpCytP450-like1,which perhaps,at least in part,contributes to betalain biosynthesis in pitaya *** information provided in the current study provides novel insights into the regulatory network associated with betalain biosynthesis during pitaya fruit coloration.

关键词： elevated activation finding

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Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening

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Horticultural Plant Journal 2020年第4期6卷 247-256页

作者： Ming Zhou Shaogui Guo Shouwei Tian Jie Zhang Yi Ren Guoyi Gong Changbao Li Haiying Zhang Yong Xu Key laboratory of Biology and genetic improvement of Horticultural Crops(North China) Beijing Key Laboratory of Vegetable Germplasm ImprovementNational Engineering Research Center for VegetablesBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China

Watermelon fruit undergoes distinct development stages with dramatic changes during fruit *** date,the molecular mechanics of watermelon ripening remain *** and transcriptome evidences suggested that the ethylene response factor(ERF)gene ClERF069 may be an important candidate factor affecting watermelon fruit *** dissect the roles of ClERF069 in fruit ripening,structure and phylogenetic analysis were performed using the amplified full-length ***-ripening watermelon 97103,non-ripening watermelon PI296341-FR and the RIL population were used to analyze ClERF069 expression dynamics and the correlation with fruit ripening *** results indicated that ClERF069 belongs to ERF family group VI and show high homology(83%identity)to melon ERF069-like ***069 expression in watermelon flesh was negatively correlated with fruit lycopene content and sugar content during fruit ripening *** transgenic evidences indicated that overexpression of 35S:ClERF069 in tomato noticeably delayed the ripening process up to 5.2 ***,β-carotenoid accumulation patterns were altered and ethylene production patterns in transgenic fruits was significantly delayed during fruit *** together,watermelon ethylene response factor ClERF069 was concluded to be a negative regulator of fruit ripening.

关键词： watermelon ethylene response factor ERF transgenic tomato ripening

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A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening

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Horticultural Plant Journal 2017年第5期3卷 190-198页

作者： WEI Wei FAN Zhongqi CHEN Jianye KUANG Jianfei LU Wangjin SHAN Wei Guangdong Provincial Key laboratory of Postharvest Science of fruits and vegetables Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China Ministry of Agriculture/Engineering Research Center of Southern Horticultural Products Preservation Ministry of Education College of Horticulture South China Agricultural University

Plant homeobox domain(PHD)-type transcription factors(TFs) are involved in a variety of biological processes. However, its involvement in commercially important fruit ripening process remains largely unclear. In the present work, the characterization of a PHD-type TF termed MaPHD1 from banana fruit is reported. Multiple alignments of the deduced amino acid sequence revealed that Ma PHD1 showed a high homology with Arabidopsis thaliana Alfin1-like proteins belonging to plant-specific sub-family of PHD finger proteins. MaPHD1 was found localized in the nucleus and exhibited trans-repression ability. It was down-regulated by ethylene and ripening. Electrophoretic Mobility Shift Assay(EMSA) and transient expression analysis demonstrated that Ma PHD1 directly bound to the G-rich motifs in the promoter of Ma XTH6, which is associated with cell wall degradation, and subsequently repressed its expression. These findings suggest that MaPHD1 may be negatively associated with banana fruit ripening, at least in part, by the direct suppression of Ma XTH6. Taken together, these findings provide new insights into the transcriptional regulatory networks of banana fruit ripening.

关键词： banana fruit ripening PHD-type transcription factor cell wall degradation transcriptional regulation

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Inhibitory effect of chitosan on growth of the fungal phytopathogen,Sclerotinia sclerotiorum,and sclerotinia rot of carrot

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Journal of Integrative Agriculture 2015年第4期14卷 691-697页

作者： WANG Qing ZUO Jin-hua WANG Qian NA Yang GAO Li-pu Beijing Vegetable Research Center Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Fruits and Vegetable Storage and Processing/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China)Ministry of Agriculture/Key Laboratory of Urban Agriculture (North)Ministry of Agriculture

The antifungal activity of chitosan on a common fungal phytopathogen, Sclerotinia sclerotiorum, and the control effect on sclerotinia rot of carrot were investigated. Mycelial growth and fungal biomass were strongly inhibited by chitosan. Using propidium iodide stain combined with fluorescent microscopy, the plasma membrane of chitosan-treated S. sclerotiorum mycelia was observed to be markedly damaged. Concomitantly, protein leakage and lipid peroxidation was also found to be significantly higher in chitosan-treated mycelia compared to the control. Chitosan provided an effective control of sclerotinia rot of carrot, with induction of activity of defense-related enzymes including polyphenoloxidase and peroxidase. These data suggest that the effects of chitosan on sclerotinia rot of carrot may be associated with the direct damage to the plasma membrane and lipid peroxidation of S. sclerotiorum, and the elicitation of defense response in carrot.

关键词： antifungal activity carrot chitosan plasma membrane Sclerotinia sclerotiorum

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Functional Characterization of Mi, a Root-knot Nematode Resistance Gene from Tomato （ Lycopersicon esculentum L.）

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Journal of Integrative Plant Biology 2006年第12期48卷 1458-1465页

作者： Ru-Gang Chen Li-Ying Zhang Jun-Hong Zhang Wei Zhang Xue Wang Bo Ouyang Han-Xia Li Zhi-Biao Ye National Key laboratory of Crop genetic improvement Wuhan 430070 China National Center for Vegetable improvement Huazhong Agricultural University Wuhan 430070 China

Root-knot nematodes （Meloidogyne spp.） cause major economic damage to numerous crop species around the world. Plant resistance is the most important attribute that is able to suppress invasion by the rootknot nematodes. In the present study, a candidate root-knot nematode resistance gene （Mi） was isolated from the resistant tomato （Lycopersicon esculentum L.） line RN-1. Expression profiling analysis revealed that this gene was expressed specifically in the roots, stems, and leaves, but not in the flowers or fruits. To verify the real function of this candidate gene, both sense and inteference RNA （RNAi） vectors were constructed. We obtained 31 transgenic plants with between one and seven copies of T-DNA inserts of sense Mi from two nematode-susceptible tomato cultivars as assayed by polymerase chain reaction （PCR） and Southern blotting analysis. Reverse transcription-PCR analysis revealed that expression levels of the Mi gene varied in different transgenic plants. Nematode assays showed that the resistance to root-knot nematodes was significantly improved in some transgenic lines compared with untransformed susceptible controls and that the resistance was heritable in selfed progeny. Loss of function via RNAi further confirmed the role of the Mi gene and the original resistant lines became susceptible to root-knot nematodes.

关键词： Mi gene overexpression RNAi root-knot nematode tomato

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Methionine oxidation and reduction of the ethylene signaling component MaEIL9 are involved in banana fruit ripening

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Journal of Integrative Plant Biology 2023年第1期65卷 150-166页

作者： Lisha Zhu Lin Chen Chaojie Wu Wei Shan Danling Cai Zengxiang Lin Wei Wei Jianye Chen Wangjin Lu Jianfei Kuang State Key laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key laboratory of Postharvest Science of fruits and vegetables/Engineering Research Center of Southern Horticultural Products Preservation Ministry of EducationCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China Key laboratory of Biology and genetic improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China

The ethylene insensitive 3/ethylene insensitive3-like(EIN3/EIL)plays an indispensable role in fruit ***,the regulatory mechanism that links post-translational modification of EIN3/EIL to fruit ripening is largely ***,we studied the expression of 13 MaE IL genes during banana fruit ripening,among which MaE IL9 displayed higher enhancement particularly in the ripening *** with its transcript pattern,abundance of MaE IL9 protein gradually increased during the ripening process,with maximal enhancement in the *** affinity purification(DAP)-seq analysis revealed that MaE IL9 directly targets a subset of genes related to fruit ripening,such as the starch hydrolytic genes MaA MY3D and MaB *** overexpressing MaE IL9 in tomato fruit hastened fruit ripening,whereas transiently silencing this gene in banana fruit retarded the ripening process,supporting a positive role of MaEIL9 in fruit ***,oxidation of methionines(Met-129,Met-130,and Met-282)in MaEIL9 resulted in the loss of its DNA-binding capacity and transcriptional activation ***,we identified MaEIL9 as a potential substrate protein of methionine sulfoxide reductase A MaMsrA4,and oxidation of Met-129,Met-130,and Met-282in MaEIL9 could be restored by ***,our findings reveal a novel regulatory network controlling banana fruit ripening,which involves MaMsrA4-mediated redox regulation of the ethylene signaling component MaEIL9.

关键词： banana EIN3/EIL redox regulation ROS starch degradation

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Transcriptomics-based identification and characterization of genes related to sugar metabolism in‘Hongshuijing’pitaya

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Horticultural Plant Journal 2022年第4期8卷 450-460页

作者： Zhike Zhang Yemiao Xing Muthusamy Ramakrishnan Canbin Chen Fangfang Xie Qingzhu Hua Jianye Chen Rong Zhang Jietang Zhao Guibing Hu Yonghua Qin State Key laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key laboratory of Postharvest Science of fruits and vegetables/Key laboratory of Biology and genetic improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037China Bamboo Research Institute Nanjing Forestry UniversityNanjing 210037China

Sugar composition not only affects fruit flavor but is also an important determinant of fruit taste and consumer *** this study,changes in the sugar content and sugar-metabolizing enzymes were investigated from different sections of various fruit development phases of‘Hongshuijing’pitaya(Hylocereus monacanthus).Genes related to sugar metabolism were also screened by transcriptome *** results indicated that glucose was the major sugar in mature pitaya fruit,and was mainly regulated by vacuolar acid invertase(VAI)and sucrose synthase(SS)(degradative direction).Sugar accumulation varied in pulp between different sections of the pitaya ***,neutral invertase(NI)and SS(degradative direction)are crucial enzymes for sugar accumulation in *** expression of 17 genes related to sucrose metabolism obtained from seven databases[NCBI non-redundant protein database(Nr),NCBI non-redundant nucleotide sequence database(Nt),EuKaryotic Orthologous Groups(KOG),The Protein Families(Pfam),Kyoto Encyclopedia of Genes and Genomes(KEGG),Swiss-prot,and Gene Ontology(GO)]were analyzed in different pitaya pulp ***1 had the highest relative expression level on the 29th day after pollination(DAP).Positive correlations were found between HpVAI1 expression and VAI activity;HpNI4 and NI activity;HpSS2,HpSS5,and SS activity(synthetic direction),indicating that HpVAI1,HpNI4,and HpSS2 and HpSS5 were involved in the regulation of VAI,NI,and SS(synthetic direction),***1 and HpNI4 regulated sucrose degradation and the accumulation of glucose and fructose,while HpSS2 and HpSS5 regulated sucrose *** results suggest that HpVAI1 plays a key role in sugar metabolism during fruit development of‘Hongshuijing’*** results of this study provide new information about sugar metabolism in pitaya fruit that could help improve fruit quality and the breeding of new cultivars.

关键词： Hylocereus monacanthus Transcriptional analysis Sugar metabolism qRT-PCR Expression analysis

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Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes

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Journal of Integrative Agriculture 2021年第6期20卷 1525-1539页

作者： JI Xiao-hao W ANG Bao-liang WANG Xiao-di W ANG Xiao-long LIU Feng-zhi WANG Hai-bo Research Institute of Pomology Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Germplasm Resources Utilization)Ministry of Agriculture/Key Laboratory of Mineral Nutrition and Efficient Fertilization for Deciduous FruitsLiaoning ProvinceXingcheng 125100P.R.China

Aroma is an important quality trait of grapes and often the focus of consumers,viticulturists and grapevine *** is a hybrid between Vitis vinifera and Vitis labrusca with a strawberry-like scent,while 87-1 is an early-ripening mutant of Muscat hamburg,belonging to Vitis vinifera,with a rose *** this study,we compared their aroma compositions and concentrations during berry development by headspace-SPME combined with gas chromatography-mass spectrometry(GC-MS),and analyzed the expression differences of enzyme-encoding genes in the LOX-HPL,MEP and MVA metabolic pathways by *** esters were detected in Kyoho during the whole berry development and they were abundant after veraison,but no esters were detected in 87-1 *** was the dominant terpene among the 14 terpenes detected in 87-1 berries,while limited amounts of terpenes were detected in Kyoho ***-PCR analysis indicated that the low expression of VvAAT might explain the low content of ester volatiles in 87-1 berries,and the low expression of coding genes in the MEP pathway,especially VvPNLin Ner1,might be the reason for the low content of volatile terpenes in Kyoho *** results from this work will promote our understanding of aroma metabolic mechanisms of grapes,and offer some suggestions for grape aromatic quality improvement.

关键词： grape Kyoho 87-1 aroma LOX-HPL MEP gene expression

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Proteomic Comparison Between Leaves from a Red-Flesh Mutant and Its Wild-Type in Sweet Orange

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Agricultural Sciences in China 2011年第8期10卷 1206-1212页

作者： PAN Zhi-yong DENG Xiu-xin National Key laboratory of Crop genetic improvement Huazhong Agricultural UniversityWuhan 430070P.R.China

The red-flesh mutant Hong Anliu sweet orange is of high nutritional value due to its lycopene *** previous studies on this mutant fruits suggested that photosynthesis and oxidative stress could promote the formation of mutation ***,leaf rather than fruit is the major part for some important biological processes such as *** this study,we analyzed the proteomic alteration in leaves of the red-flesh mutant Hong Anliu *** wild type （WT）.Ten differentially expressed proteins were identified,of which two were involved in photosynthesis,three in oxidative stress,two in defense,and three in *** high up-regulation of photosynthetic proteins proved the hypothesis that enhanced photosynthesis could provide and transport more substrates into mutant fruits for carotenoid *** to the previous results in fruits,anti-oxidative proteins were highly up-regulated in leaves,suggesting the whole plant of Hong Anliu suffered from enhanced oxidative *** involved in defense and metabolism were also identified,and their possible roles in the mutation were discussed.

关键词： sweet orange lycopene accumulation proteomic photosynthesis oxidative stress

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