A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening

作     者：WEI Wei FAN Zhongqi CHEN Jianye KUANG Jianfei LU Wangjin SHAN Wei 

作者机构：Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and VegetablesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China Ministry of Agriculture/Engineering Research Center of Southern Horticultural Products Preservation Ministry of Education College of Horticulture South China Agricultural University 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2017年第3卷第5期

页      面：190-198页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the China Agriculture Research System (Grant No. CARS-32-09) 

主　　题：banana fruit ripening PHD-type transcription factor cell wall degradation transcriptional regulation 

摘      要：Plant homeobox domain(PHD)-type transcription factors(TFs) are involved in a variety of biological processes. However, its involvement in commercially important fruit ripening process remains largely unclear. In the present work, the characterization of a PHD-type TF termed MaPHD1 from banana fruit is reported. Multiple alignments of the deduced amino acid sequence revealed that Ma PHD1 showed a high homology with Arabidopsis thaliana Alfin1-like proteins belonging to plant-specific sub-family of PHD finger proteins. MaPHD1 was found localized in the nucleus and exhibited trans-repression ability. It was down-regulated by ethylene and ripening. Electrophoretic Mobility Shift Assay(EMSA) and transient expression analysis demonstrated that Ma PHD1 directly bound to the G-rich motifs in the promoter of Ma XTH6, which is associated with cell wall degradation, and subsequently repressed its expression. These findings suggest that MaPHD1 may be negatively associated with banana fruit ripening, at least in part, by the direct suppression of Ma XTH6. Taken together, these findings provide new insights into the transcriptional regulatory networks of banana fruit ripening.