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Differentially expressed miRNAs in anthers may contribute to the fertility of a novel brassica napus genic male sterile line CN12A

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Journal of Integrative agriculture 2020年第7期19卷 1731-1742页

作者： DONg Yun WANg Yi JIN Feng-wei XINg Lijuan FANg Yan ZHANg Zheng-ying ZOU Jun-jie WANg Lei XU Miao-yun Crop Research Institute Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China National Key Facility for Crop gene Resources and genetic Improvementbiotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081P.R.China gansu Provincial Key Lab of Aridland Crop Sciences/gansu Agricultural university Lanzhou 730070.P.R.China

In brassica napus L.(rapeseed),complete genic male sterility(gMS)plays an important role in the utilization of *** microRNAs(miRNAs)play essential regulatory roles during bud development,knowledge of how gMS is regulated by miRNAs in rapeseed is rather *** this study,we obtained a novel recessive gMS system,*** sterile line CN12A has defects in tapetal diferentiation and *** sequencing was employed to examine the expression of miRNAs in the buds of CN12A and the fertile line *** identifed 85 known miRNAs and 120 novel miRNAs that were expressed during rapeseed anther *** comparing the expression levels of miRNAs between CN12A and CN12b,19 and 18 known miRNAs were found to be difrentially expressed in 0.5--1.0 mm buds and in 2.5--3.0 mm buds,*** these,the expression levels of 14 miRNAs were higher and the levels of 23 miRNAs were lower in CN12A compared with *** predicted target genes of these iferentially expressed miRNAS encode protein kinases,F-box domain-containing proteins,MADS-box family proteins,SbP-box gene family members,HD ZIP proteins,floral homeotic protein APETAL A 2(AP2)。and nuclear factor Y,subunit *** targets have previously been reported to be involved in pollen development and male sterility,suggesting that miRNAs might act as regulators of gMS in rapeseed ***,RT-qPCR data suggest that one of the dffrentially expressed miRNAs,bna-miR159,plays a role in tapetal differentiation by regulating the expression of transcription factor bnMYb101 and participates in tapetal degradation and infuences callose degradation by manipulating the expression of *** findings contibute to our understanding of the roles of miRNAs during anther development and the occurrence of gMS in rapeseed.

关键词： brassica napus L. genic male sterility miRNAs targets molecular regulation network

Marginal revenue of land and total factor productivity in Chinese agriculture： Evidence from spatial analysis

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Journal of geographical Sciences 2012年第1期22卷 167-178页

作者： HOU Linke ZHANg Yanjie ZHAN Jinyan Thomas gLAUbEN Institute of geographic Sciences and Natural Resources Research CAS Leibniz Institute of Agricultural Development in Central and Eastern Europe(IAMO) State Key Laboratory of Water Environment Simulation School of EnvironmentBeijing Normal University

This paper attempts to explore the temporal and spatial nature of the marginal revenue of land, total factor productivity （TFP） change and its three components： technical change （TC）, technical efficiency change （TEC） and scale efficiency change （SEC） as seen in Chinese agricultural production from 1995 to 1999. based on county-level data, the study utilized both stochastic frontier and mapping analyses methods. The results show that growth in the marginal revenue of land was diverse across various regions, where most gain occurred in eastern coastal zone, while loss was in Northwest and North China. China has experienced moderate decreases in annual TFP change （–0.26%） with considerable regional variations. Specifically, the administrative intervention in grain production and the deterioration of the agricultural technology diffusion system led to a moderate drop in annual TFP change. County-level mapping analyses took into account interregional variances in TFP and its components. Regarding components of TFP, TEC differences explain the majority of regional dispersions in TFP. As developed areas in China, the Huang-Huai-Hai region and the beijing-Tianjin-Tangshan economic zone face the challenges of land conversion and grain security amidst the process of urbanization.

关键词： TFP change grain production technology efficiency change Chinese agriculture

Tea and Citrus maxima complex induces apoptosis of human liver cancer cells via PI3K/AKT/mTOR pathway in vitro

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Chinese Herbal Medicines 2022年第3期14卷 449-458页

作者： Shuai Wen Ran An Dongli Li Junxi Cao Zhigang Li Wenji Zhang Ruohong Chen Qiuhua Li Xingfei Lai Lingli Sun Shili Sun Tea Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation&UtilizationGuangzhou510640China School of biotechnology and Health Sciences Wuyi UniversityJiangmen 529020China International Healthcare Innovation Institute(Jiangmen) Jiangmen 529040China

Objective:In this study,black tea and Citrus maxima(bT-CM),yellow tea and ***(YT-CM),green tea and ***(gT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and ***(T-CM)were *** effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further ***:National standard method and HPLC were used for active ingredient ***,cell fow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation,apoptosis,and its underlying molecular ***:The content of tea polyphenols,free amino acids,ratio of polyphenols and amino acids,ester catechins,non-ester catechins and caffeine in YT-CM and gT-CM was significantly higher than that of *** in vitro antioxidant capacity of YT-CM and gT-CM was also significantly stronger than that of *** T-CMs had the effects of inhibiting proliferation,arresting cell cycle and inducing apoptosis in Hepg2 and bel7402 cells,especially YT-CM and *** blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins bax,bcl-2 and Caspase-3/***-CM and gT-CM had better ability to change the signal pathway than ***:In short,T-CMs,which combined different degrees of fermentation tea with ***,were rich in nutrients and biologically active substances.T-CMs,especially YT-CM and gT-CM,are healthy drinks that help to prevent and treat liver cancer.

关键词： apoptosis Citrus maxima cv.Tomentosa liver cancer signaling pathway tea

Problems and Development Countermeasures of Agricultural Water-Saving Irrigation

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Meteorological and Environmental Research 2018年第3期9卷 101-104,108页

作者： Zhang Dan Liaoning Institute of Water Resources and Hydropower Research Shenyang 110003China College of Water Conservancy Shenyang Agricultural UniversityShenyang 110161China

agriculture is the biggest water user in China,and the development of agricultural water-saving has great significance to the national economy and social development. In this paper,the present situation of water used in agriculture irrigation and water-saving potential were analyzed,and the ＂ bottleneck＂ and main problems existing in water-saving irrigation in China were discussed. From the aspects of engineering investment channels,agricultural water-saving policies and management system,reform of agricultural water price and water right transfer,improvement of farmers＇ water-saving consciousness,and promotion of rural land transfer,suggestions were proposed for the development of China＇s agricultural water-saving in future,which will provide a technical support for the sustainable use of agricultural water resources in China.

关键词： Agricultural water-saving Water-saving potential Agricultural water rights

Effects of Artificial Aging on Physiological Characteristics and Seed Vigor of Medicago ruthenica Seeds

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Animal Husbandry and Feed Science 2009年第6期1卷 38-41页

作者： WANg Xiao-li LI Hong-yan LI Zhi-yong SHI Wen-gui Pratacultural College Gansu Agricultural University Lanzhou 730070 China grassland Research Institute Chinese Academy of Agricultural Sciences Hohhot 010010 China

As shown by the changes in physiological characteristics and seed vigor of three Medicago ruthenica samples during artificial aging process, the germination potential, germination rate, germination index, vigor index and simple vigor index declined with the extension of aging time on the whole. After artificial aging for 6 min, the vigor was higher than that of the control. The conductivity, malondialdehyde content and soluble polysaccharide content of seed leachate increased with the aging time and negatively correlated （ P 〈 0.05） with the seed vigor indicators. The physiological indicators were very significantly correlated （P 〈 0.01 ） with the vigor indicators in the Medicago ruthenica sample from Dorbod Qi, UIanqab City（China）.

关键词： Medic.ago ruthenica Artificial aging Seed vigor Physiological characteristics

Heterologous expression of the glucose oxidase gene in Trichoderma atroviride leads enhanced ability to attack phytopathogenic fungi and induction of plant systemic disease resistance

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浙江大学学报（农业与生命科学版） 2004年第4期30卷 418-418页

作者： Robert L Mach brunner Kurt Matteo Lorito Susanne Zeilinger Rosalia Ciliento Sheridan Woo gene technology group Institute of Chemical Engineering TU-Vienna Getreidemarkt 9/166/5 A-1060 Wien Austria Gene Technology Group Institute of Chemical Engineering TU-Vienna Getreidemarkt 9/166/5 A-1060 Wien AustriaDipartimento di Arboricoltura Botanica e Patologia Vegetale Sezione di. Patologia Vegetale Università degli Studi di Napoli "Federico II" Via Università 100 80050. Portici (NA) Italy Gene Technology Group Institute of Chemical Engineering TU-Vienna Getreidemarkt 9/166/5 A-1060 Wien AustriaDipartimento di Arboricoltura Botanica e Patologia Vegetale Sezione di. Patologia Vegetale Università degli Studi di Napoli "Federico II" Via Università 100 80050. Portici (NA) Italy Dipartimento di Arboricoltura Botanica e Patologia Vegetale Sezione di. Patologia Vegetale Università degli Studi di Napoli "Federico II" Via Università 100 80050. Portici (NA) Italy

A transgenic strain of Trichoderma atroviride that expresses the Aspergillus niger glucose oxidase gene goxA under a homologous pathogen-inducible promoter (nag1) has been constructed, with the aim of increasing the ability of this biocontrol agent (bCA) to attack phytopathogenic fungi and enhance plant systemic disease resistance. The sporulation and growth rate of the transgenic progenies were similar to the wild-type strain P1. goxA expression occurred immediately after contact with the plant pathogen, and the glucose oxidase formed was secreted extracellularly. The transformed strain SJ3 4, containing 12-14 copies of the transgene, produced significantly less N-acetyl-glucosaminidase and endochitinase then wild type. However, the ability of its culture filtrate to inhibit the germination of botrytis cinerea spores was increased by about 3-fold. In comparison to P1, the transgenic strain more quickly overgrew and lysed in vitro the pathogens Rhizoctonia solani and Pythium ultimum. In assays in vivo SJ3 4 showed a highly improved biocontrol ability in soil heavily infested with those pathogens, where the wild type was unable to protect the plant and allow seeds to germinate. The Trichoderma-gox was able to induce a much higher level of systemic resistance against the foliar pathogen b. cinerea, as compared to the parent strain. This work demonstrate that i) heterologous genes driven by pathogen-inducible promoters can improve the biocontrol and Induced Systemic Resistance properties of fungal bCAs such as Trichoderma spp., and ii) these microbes can be used as vectors to provide the plant with useful molecules able, for instance, to increase pathogen

关键词：植物致病真菌抗性病害植物异种表达葡萄糖氧化酶基因

Differentially Expressed genes Related to Fertility Conversion in Thermo-sensitive genic Male Sterile(TgMS) Lines of brassica juncea

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Agricultural Science & technology 2017年第10期18卷 1795-1799,1804页

作者： Libo SUI Haiyang ZHAO Lijun AI Liping LI Chuanli ZHANg Chao SUN Tianxiang TANg Liangbin LIN College of Agronomy and biotechnology Yunnan Agricultural University Kunming 650201 China The Center for basic Agricultural Experiments and Teaching Yunnan Agricultural University Kunming 650201 China College of Tropical Crops Yunnan Agricultural University Pu＇er 665000 China

[Objective] This study was performed to screen functional genes related to the fertility conversion of thermo-sensitive genic male sterile （TgMS） lines of brassica juncea L. [Method] A b. juncea TgMS line K121S was selected as the experimental material. The total RNAs were isolated from fertile and sterile pollens at different development stages, including mother cell stage, tetrad stage, tricellular pollen stage and maturity stage. DDRT-PCR was carried out to identify differentially expressed genes. [Result] A total of 44 differentially expressed cDNA fragments were identified with Dot blot. And seven candidate genes related to fertility conversion of K121S were screened out by bLASTN, including callose synthase gene, aldehyde dehydrogenase gene and RNA polymerase I transcription factor RRN3 gene which were differentially expressed at the transcriptional level, H＇-ATPase gene, fructose diphosphate aldolase -class I gene, teucine-rich repeat receptor-Jike serine/threonine- protein kinase gene and alkaline/neutral invertase gene, which were differentially expressed at the post-transcriptional level. [Conclusion] The results of this study will help to explain the molecular mechanism of thermo-sensitive genic male sterility of b. juncea.

关键词： brassica juncea L. Thermo-sensitive male genic sterility DDRT-PCR Fertility conversion related genes

A Review and Recommendations for Promoting Environmentally-Friendly agriculture in Korea

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Journal of Agricultural Science and technology(b) 2017年第3期7卷 139-146页

作者： Chang-gil Kim Song Soo Lim The Korea Rural Economic Institute Naju 58321 Korea Department of Food & Resource Economics College of Life Sciences and Biotechnology Korea University Seoul 02841 Korea

The objective of this study was to provide a macroscopic evaluation review of environmentally-friendly agriculture （EFA） status and then suggest policy recommendations for promoting EFA in Korea. Amid considerable challenges, including food security, many small-scale farms with increasingly aged farm population and growing competition, agri-environmental indicators （AEIs） and EFA certificate performance shed light on the fact that the trend of the state of the environment reversed, albeit with room to improve. Identifying positive contributions to EFA made by target-oriented long-term EFA plans and the direct payment scheme, this paper suggests to enrich the agricultural information systems, a system approach to agri-environmental resource management, and make a transition toward the low inputs farming systems.

关键词： Environmentally-friendly agriculture agri-environmental indicators nutrient balance direct payments low inputs farming system best management practice.

Prehistoric demographic fluctuations in China inferred from radiocarbon data and their linkage with climate change over the past 50,000 years

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Prehistoric demographic fluctuations in China inferred from ...