Tea and Citrus maxima complex induces apoptosis of human liver cancer cells via PI3K/AKT/mTOR pathway in vitro

作     者：Shuai Wen Ran An Dongli Li Junxi Cao Zhigang Li Wenji Zhang Ruohong Chen Qiuhua Li Xingfei Lai Lingli Sun Shili Sun Shuai Wen;Ran An;Dongli Li;Junxi Cao;Zhigang Li;Wenji Zhang;Ruohong Chen;Qiuhua Li;Xingfei Lai;Lingli Sun;Shili Sun

作者机构：Tea Research InstituteGuangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation&UtilizationGuangzhou510640China School of Biotechnology and Health SciencesWuyi UniversityJiangmen 529020China International Healthcare Innovation Institute(Jiangmen)Jiangmen 529040China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2022年第14卷第3期

页      面：449-458页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the"14th Five-Year Plan"teambuilding projects of Guangdong Academy of Agricultural Sciences[202126TD] Guangdong Basic and Applied Basic Research Foundation[2020A1515011266,2021A1515010958] Guangzhou Science and Technology Plan Projects Key-Area Research and Development Program of Guangdong Province[2020B0202080003] Innovation Fund projects of Guangdong Academy of Agricultural Sciences Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science[R2019PY-JX004] 

主　　题：apoptosis Citrus maxima cv.Tomentosa liver cancer signaling pathway tea 

摘      要：Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and ***(YT-CM),green tea and ***(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and ***(T-CM)were *** effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further ***:National standard method and HPLC were used for active ingredient ***,cell fow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation,apoptosis,and its underlying molecular ***:The content of tea polyphenols,free amino acids,ratio of polyphenols and amino acids,ester catechins,non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of *** in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of *** T-CMs had the effects of inhibiting proliferation,arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells,especially YT-CM and *** blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax,Bcl-2 and Caspase-3/***-CM and GT-CM had better ability to change the signal pathway than ***:In short,T-CMs,which combined different degrees of fermentation tea with ***,were rich in nutrients and biologically active substances.T-CMs,especially YT-CM and GT-CM,are healthy drinks that help to prevent and treat liver cancer.