检索结果-南通市图书馆

Pien Tze Huang Inhibits Migration and Invasion of Hepatocellular Carcinoma Cells by Repressing PDGFRB/YAP/CCN2 Axis Activity

引用

Chinese Journal of Integrative Medicine 2024年第2期30卷 115-124页

作者： LUO Zhi-yi TIAN Qi CHENG Niang-mei LIU Wen-han YANG Ye CHEN Wei ZHANG Xiang-zhi ZHENG Xiao-yuan CHEN Ming-sheng ZHUANG Qiu-yu ZHAO Bi-xing LIU co.g-sheng LIU Xiao-long LI Qin WANG Ying-chao The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhou(350025)China fujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development Zhangzhou Pien Tze Huang Pharmaceutical Co.Ltd.ZhangzhouFujian Province(363099)China co.lege of Biological Science and Engineering and Mengchao Med-X Center Fuzhou UniversityFuzhou(350116)China Department of Internal Medicine Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhou(350025)China

Objective:To investigate the effects of Pien Tze Huang(PZH) on the migration and invasion of HCC cells and underlying molecular ***:Cell co.nting kit-8(CCK-8) was applied to evaluate the cell viabilities of SMMC-7721,SK-Hep-1,C3A and HL-7702(6 × 10^(3)cells/well) co.incubated with different co.centrations of PZH(0,0.2,0.4,0.6,0.8 mg/mL) for 24 ***,wound healing assay,CCK-8and Annexin V-FITC/PI staining were co.ducted to investigate the effects of PZH on the migration,invasion,proliferation and apoptosis of SK-Hep-1 and SMMC-7721 cells(650 μg/mL for SK-Hep-1 cells and 330 μg/mL for SMMC-7721 cells),*** vivo,lung metastasis mouse model co.structed by tail vein injection of HCC cells was used for evaluating the anti-metastasis function of ***-Hep-1 cells(10^(6)cells/200 μL per mice) were injected into B-NDG mice via tail *** 8 mice were randomly divided into PZH and co.trol groups,4 mice in each *** 2-d inoculation,mice in the PZH group were administered with PZH(250 mg/kg,daily) and mice in the co.trol group received only vehicle(PBS) from the 2nd day after xenograft to day *** analysis based on RNA-seq was subsequently used for deciphering anti-tumor mechanism of *** real-time polymerase chain reaction(qRT-PCR) and Western blot were applied to verify RNA-seq *** reporter assay was performed to examine the transcriptional activity of yes-associated protein(YAP).Results:PZH treatment significantly inhibited the migration,invasion,proliferation and promoted the apoptosis of HCC cells in vitro and in vivo(Pco.nective tissue growth factor(CCN2),N-cadherin,vimentin and matrix metallopeptidase 2(MMP2,P<0.01).Meanwhile,the phosphorylatio

关键词： PienTzeHuang hepatocellular carcinoma RNA-seq Hippo yes-associated protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phase separation of Nur77 mediates XS561-induced apoptosis by promoting the formation of Nur77/Bcl-2 co.densates

引用

Acta Pharmaceutica Sinica B 2024年第3期14卷 1204-1221页

作者： Xiaohui Chen Meichun Gao Yongzhen Xia Xin Wang Jingbo Qin Hongying He Weirong Liu Xiaowei Zhang Shuangzhou Peng Zhiping Zeng Ying Su Xiaokun Zhang School of pharmaceutical Science Fujian Provincial Key Laboratory of Innovative Drug Target ResearchXiamen UniversityXiamen 361002China NucMito pharmaceuticals co. Ltd.Xiamen 361000China Department of Clinical Laboratory the Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000China

The orphan nuclear receptor Nur77 is a critical regulator of the survival and death of tumor *** pro-death effect of Nur77 can be regulated by its interaction with Bcl-2,resulting in co.version of Bcl-2 from a survival to *** Bcl-2 is overexpressed in various cancers preventing them from apoptosis and promoting their resistance to chemotherapy,targeting the apoptotic pathway of Nur77/Bcl-2 may lead to new cancer ***,we report our identification of XS561 as a novel Nur77 ligand that induces apoptosis of tumor cells by activating the Nur77/Bcl-2 *** vitro and animal studies revealed an apoptotic effect of XS561 in a range of tumor cell lines including MDA-MB-231 triple-negative breast cancer(TNBC)and MCF-7/LCC2 tamoxifen-resistant breast cancer(TAMR)in a Nur77-dependent *** studies showed XS561 potently induced the translocation of Nur77 from the nucleus to mitochondria,resulting in mitochondria-related ***,XS561-induced accumulation of Nur77 at mitochondria was associated with XS561 induction of Nur77 phase separation and the formation of Nur77/Bcl-2 ***,our studies identify XS561 as a new activator of the Nur77/Bcl-2 apoptotic pathway and reveal a role of phase separation in mediating the apoptotic effect of Nur77 at mitochondria.

关键词： XS561 Nur77 Bcl-2 Apoptosis Mitochondria Phase separation TNBC TAMR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

from Gancao Qinlian Extract and Study of Its Efficacy Screening of Anti-inflammatory Active Ingredients from Gancao Qinlian Extract and Study of Its Efficacy

引用

Medicinal Plant 2024年第4期15卷 4-10页

作者： Maixun ZHU Yang ZHANG Yue XU Hongmei TANG Tao WU Yanda ZHANG National Center of Technology Innovation for Pigs Chongqing 402460China Chongqing Academy of Animal Sciences Chongqing 402460China fujian Bedi pharmaceutical co. Ltd.NingdeFujian402460China

[Objectives]To establish the chromatographic fingerprint of Gancao Qinlian Extracts(GQE)and reveal the possible material basis of the anti-inflammatory effect of GQE by the co.relation analysis between the fingerprint chromatographic peaks of different co.ponents of GQE and its anti-inflammatory activity.[Methods]Ultra-performance liquid chromatography(UPLC)was used to detect the different ingredients of GQE to establish its chromatographic fingerprint and analyze the differences among the three medicine co.ponents;LPS stimulated RAW264.7 cells to co.struct an inflammatory cell *** NO secretion of cells was detected by the Griess *** was used to detect the changes in TNF-αand IL-10 ***-qPCR tested the mRNA expression levels of TNF-αand *** relational analysis was carried out by co.bining fingerprint chromatographic peak data and anti-inflammatory activity data.[Results]The GQE fingerprint was established,34 fingerprint characteristic peaks were calibrated,and 33 related chromatographic peaks were screened *** co.responding chromatographic peaks in the three co.ponents were obtained,and the co.tent of the co.ponents was calculated;the anti-inflammatory results showed that the co.tent of NO,TNF-α,and the expression of TNF-αmRNA in the high and medium-dose groups of GQE were significantly lower than those in the blank group(Pco.tent and TNF-αmRNA expression in the high-dose group of GQE I was co.siderably lower than those in the blank group(Pco.relation degree of the three co.ponents was GQE II>GQE>GQE I,and the characteristic fingerprint peaks 12,15,22,23,28,31,33 may be closely related to the anti-inflammatory effect.[co.clusions]The best co.ponent of the anti-inflammatory effect in GQE is w

关键词： Gancao Qinlian Extracts Anti-inflammatory activity Fingerprint Grey relational analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Deciphering the high-carbonization-temperature triggered enzymatic activity of wool-derived N,S-co.doped carbon nanosheets

引用

Chinese Chemical Letters 2024年第3期35卷 182-188页

作者： Jiachen Zhao Deshuai Yu Jianmin Chen Shihao Lin Yonghua Tang Chaoyu Fan Dongfang Zhou Youhui Lin Department of Physics Research Institute for Biomimetics and Soft MatterFujian Provincial Key Laboratory for Soft Functional Materials ResearchXiamen UniversityXiamen 361005China School of Pharmacy and Medical Technology Putian UniversityPutian 351100China Key Laboratory of Mental Health of the Ministry of Education NMPA Key Laboratory for Research and Evaluation of Drug Metabolism&Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Key Laboratory of pharmaceutical Analysis and Laboratory Medicine(Putian University) Fujian Province UniversityPutian 351100China

It is well-established that high carbonization temperature will trigger the enzyme-like activity of carbon-based ***,the catalytic mechanism is still ambiguous,which hinders the further rational design of nanomaterials as enzyme ***,N,S-rich carbonized wool nanosheets(CWs)were synthesized at different pyrolysis *** expected,only CWs treated with high-temperature possess intrinsic oxidase-and peroxidase-like ***,density functional theory(DFT)calculations demonstrate that graphitic nitrogen and the co.existence of nitrogen and sulfur in the carbon matrix serve as the active sites for the enzyme-like *** importantly,co.bining theoretical calculations and experimental observations,the high-temperature triggered catalytic mechanism can be ascribed to the fact that an appropriate high-temperature maximizes the graphitization degree to a certain extent,at which most of the catalytic active sites are well retained rather than ***,co.pling with excellent photothermal co.version efficiency and catalytic performance,CWs can be applied to photothermal-catalytic cancer therapy under near-infrared region(NIR)light *** believe this work will co.tribute to understanding the catalytic mechanism of carbon-based nanozymes and promote the development of new biomedical and pharmaceutical applications.

关键词： Carbon-based nanozyme Catalytic mechanism Density functional theory High-temperature carbonization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Therapeutic Effects of Different Animal Bile Powders on Lipid Metabolism Disorders and Their co.position Analysis

引用

Chinese Journal of Integrative Medicine 2022年第10期28卷 918-923页

作者： CHEN Da-xin CHU Jian-feng LIN Shan ZHANG Ling CHEN Hong-wei SUN Zhi-wei XU Jian-feng CAI Qiao-yan WANG Li-li PENG Jun Academy of Integrative Medicine Cardiovascular Research Institute Fuzhou(350122)China fujian Key Laboratory of Integrative Medicine on Geriatric Fujian University of Traditional Chinese MedicineFuzhou(350122)China Chen Ke-ji Academic Thought Heritage Studio Fuzhou(350122)China fujian guizhentang pharmaceutical co. Ltd.HuianFujian Province(362142)China

Objective: To co.pare the therapeutic effect of different animal bile powders on lipid metabolism disorders induced by high-fat diet in rats, and analyze the bioactive co.ponents of each animal bile powder. Methods: Sixty Sprague-Dawley rats were randomly divided into 6 groups(n=10): normal diet co.trol group, high-fat diet model group, high-fat diet groups orally treated with bear, pig, co. and chicken bile powders, respectively. Serum biochemical markers from the abdominal aorta in each group were analyzed. Changes in the body weight and liver weight were reco.ded. Pathohistological changes in the livers were examined. High performance liquid chromatography co.pled with quadrupole time-of-flight tandem mass spectrometry was used to determine the co.position of bioactive co.ponents in each animal bile powder. Results: Treatment with different types of animal bile powders had different inhibitory effects on high-fat diet-induced increase of body weight and/or liver weight in rats, most notably in bear and pig bile powders(Pco.ld be reversed by treatment with all kinds of bile powders. Bear bile and chicken bile showed the most potent therapeutic effect against lipid metabolism disorder. co. and bear bile effectively alleviated high-fat diet induced liver enlargement and disco.oration, hepatocyte swelling, infiltration of inflammatory cells and formation of lipid vacuoles. Bioactive co.ponent analysis revealed that there were significant differences in the relative co.tent of taurocholic acid, taurodeoxycholic acid and ursodeoxycholic acid among different types of animal bile. Interestingly, a unique co.ponent with molecular weight of 496.2738 Da, whose function has not yet been reported, was identified only in bear bile powder. co.clusions: Different animal bile powders had varying therapeutic effect against lipid metabolism disorders induced by high-fat diet, and bear bile powder demonstrated th

关键词： bile powders lipid metabolism active ingredient liver

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Traditional Chinese medicine Pien-Tze-Huang ameliorates LPS-induced sepsis through bile acid-mediated activation of TGR5-STAT3-A20 signalling

引用

Journal of pharmaceutical Analysis 2024年第4期14卷 601-614页

作者： Bei Li Yong Zhang Xinyuan Liu Ziyang Zhang Shuqing Zhuang Xiaoli Zhong Wenbo Chen Yilin Hong Pingli Mo Shuhai Lin Shico.g Wang Chundong Yu State Key Laboratory of Cellular Stress Biology School of Life SciencesXiamen UniversityXiamen361102China fujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development ZhangzhouFujian363000China Xiang'an Hospital of Xiamen University School of MedicineXiamen UniversityXiamen361005China Department of Cardiology Xiamen Key Laboratory of Cardiac ElectrophysiologyXiamen Institute of Cardiovascular DiseasesThe First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen361003China

Pien Tze Huang(PZH),a class-1 nationally protected traditional Chinese medicine(TCM),has been used to treat liver diseases such as hepatitis;however,the effect of PZH on the progression of sepsis is ***,we reported that PZH attenuated lipopolysaccharide(LPS)-induced sepsis in mice and reduced LPS-induced production of proinflammatory cytokines in macrophages by inhibiting the activation of mitogen-activated protein kinase(MAPK)and nuclear factor-kappa B(NF-κB)***,PZH stimulated signal transducer and activator of transcription 3(STAT3)phosphorylation to induce the expression of A20,which co.ld inhibit the activation of NF-κB and MAPK *** of the bile acid(BA)receptor G protein-co.pled bile acid receptor 1(TGR5)in macrophages abolished the effects of PZH on STAT3 phosphorylation and A20 induction,as well as the LPS-induced inflammatory response,suggesting that BAs in PZH may mediate its anti-inflammatory effects by activating ***,deprivation of BAs in PZH by cholestyramine resin reduced the effects of PZH on the expression of phosphorylated-STAT3 and A20,the activation of NF-κB and MAPK signalling,and the production of proinflammatory cytokines,whereas the addition of BAs to cholestyramine resin-treated PZH partially restored the inhibitory effects on the production of proinflammatory ***,our study identifies BAs as the effective co.ponents in PZH that activate TGR5-STAT3-A20 signalling to ameliorate LPS-induced sepsis.

关键词： Pien Tze Huang Bile acids Sepsis Inflammation TGR5 STAT3-A20 signalling

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Novel ZIF-8/ZnS hollow polyhedral heterostructures derived from ZIF-8 with enhanced photocatalytic activity for degradation of aflatoxin B1

引用

Progress in Natural Science:Materials International 2023年第5期33卷 575-580页

作者： Junjie Pan Xiaobing Yang Junmei Zhou Wenliang Cheng Kejun Cheng Chemical Biology Center Lishui Institute of Agriculture and Forestry Sciences fujian Provincial Key Laboratory of Eco.Industrial Green Technology Wuyi University Zhejiang Kangning pharmaceutical co. Ltd

Myco.oxins,formed in lots of foods during the production,processing and transportation processes,are the seco.dary metabolites of Fusarium genus,Penicillium and *** B1(AFB1) is co.sidered as one of the most dangerous myco.oxins for human and animals due to its strong carcinogenicity and hepatotoxic ***,degradation of AFB1co.pletely or reduction of AFB1to a low degree co.tent has caused much *** this paper,novel ZIF-8/ZnS hollow polyhedral heterostructures were successfully synthesized by sulphurizing ZIF-8 via solvothermal *** synthesized samples were used as catalysts for AFB1degradation under UV light *** as-synthesized samples were analyzed by XRD,SEM,BET,UV-vis,ESR and so *** results show that parts of ZIF-8 can be sulphurized by TAA to synthesize ZnS and the ZIF-8 particles were etched to form the hollow *** sulphurizing degree of ZIF-8 has great influence on the photocatalytic activity of ZIF-8/*** with ZIF-8/ZnS(50) and ZIF-8/ZnS(150),ZIF-8/ZnS(100) exhibited the best photocatalytic activity and co.ld degrade 98.6 % of *** that,the reactive species of ZIF-8/ZnS(100) were co.firmed to be ·OH and ·O2-.This strategy of co.pling MOFs with semico.ductor provides a facile method to synthesize for catalyst with enhanced photocatalytic activity for degradation of food pollutants produced by aflatoxins.

关键词： Photocatalytic activity

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Bear Bile Powder(熊胆粉) Induces Apoptosis of Human Hepatocellular Carcinoma Cells via Mitochondrion-Dependent Pathway

引用

Chinese Journal of Integrative Medicine 2014年第2期20卷 123-129页

作者：赵锦燕陈志鸿林薇钟小勇陈旭征彭军洪振丰 fujian Academy of Integrative Medicine fujian Key Laboratory of Integrative Medicine on Geriatrics Fujian University of Traditional Chinese Medicine fujian guizhentang pharmaceutical co. Ltd.

Objective： To evaluate the effect of Bear Bile Powder （熊胆粉, BBP） on the growth and apoptosis of HepG2 human hepatocellular carcinoma cells, and investigate the possible molecular mechanisms mediating its anti-cancer activity. Methods： HepG2 cells were treated with 0.4-1.0 mg/mL of BBP for 24, 48 and 72 h. The viability of HePG2 cells was determined by MTT assay. Cellular morphology was observed via phase-co.trast microsco.y. Fluorescence-activated cell sorting analysis with Annexin-V/propidium idodide and 5,5＇,6,6＇-tetrachloro- 1 ,1＇,3,3＇-tetraethyl-benzimidazol-carbocyanine iodide （JC-1） staining was performed to determine cell apoptosis and the loss of mitochondrial membrane potential, respectively. Activation of caspase-9 and -3 was evaluated by a co.orimetric assay. Results： The treatment with 0.4-1 mg/mL of BBP for 24, 48, or 72 h respectively reduced cell viability significantly by 7%-60%, 20%-90% or 25%-98%, co.pared with the untreated co.trol cells （P〈0.01）. In addition, BBP treatment induced morphological changes in HepG2 cells. Furthermore, after treated with 0, 0.4, 0.6, 0.8 and 1.0 mg/mL of BBP, apoptosis cells （including early and late apoptotic cells） were 18.0% ± 1.3%, 34.9% ± 2.2%, 33.9% ± 2.8%, 37.4% ± 2.8% and 46.0% ± 2.5%, respectively （P〈0.05）; and the percentage of cells with reduced JC-1 red fluorescence were 6.6% ± 0.8%, 8.5% ± 0.8%, 13.5% ± 1.6%, 17.6%± 2.3% and 46.7% ± 3.6%, respectively （P〈0.01）. Finally, BBP treatment significantly and dose-dependently induced activation of both caspase-9 and caspase-3 in HepG2 cells （P〈0.05）. co.clusions： BBP co.ld inhibit the growth of HepG2 hepatocellular cancer cells through mitochondrion-mediated apoptosis, which may, in part, explain its anti-cancer activity. BBP may be a potential novel therapeutic agent for the treatment of hepatocellular carcinoma.

关键词： Bear Bile Powder hepatocellular carcinoma apoptosis mitochondria

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An Efficient Semi-Synthetic Method to co.struct Docetaxel via Sterically Crowded Linear Side Chain Esterification

引用

Chinese Journal of Chemistry 2013年第1期31卷 31-36页

作者： Xin Shen Jidong Yang Huaxing Zhan Hu Wang Shaohong Wu Zili Chen Shanghai Parling PharmaTech co. Ltd. Suite 2 No. 868 Zhenchen Road Baoshan District Shanghai 200444 China fujian South pharmaceutical co. Ltd. 98# Dong-Xin Road Xuefeng Town Mingxi County Fujian 365200 China Department of Chemistry Renmin University of China Beijing 100872 China

An efficient semi-synthetic method was developed to co.struct docetaxel 1 by using N,N-di-Boc protected lin- ear isoserine derivative 5 as the side chain source, in which, bulky protecting group on the nitrogen atom b... 详细信息

关键词： Docetaxel semi-synthesis lO-deacetylbaccatin III esterification

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Separation of injectable salidroside by co.umn chromatography of macroporous resins for treating myocardial ischemia

引用

Science China Chemistry 2012年第7期55卷 1435-1444页

作者： JU RuiJun HUANG RenJie ZHOU Jia LI RuoJing ZHOU Peng ZHANG ZaoHua XIANG FeiJun XU DongJin LIU WeiXiang MA XingTian ZHANG Qiang LU WanLiang State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Department of Pharmacy Fujian Health CollegeFuzhou 350101China China Academy of Chinese Medical Sciences Beijing 100700China Kangmei pharmaceutical co. Ltd.Puning 515300China

The objective of the present study is to develop a method for large-scale separating and purifying salidroside from rhodiola kirilowii roots and for preparing injectable medicinal *** extract of salidroside was prepared by water-ethanol system,and purified by co.umn chromatography of macroporous *** adsorption and desorption studies were performed on six kinds of macroporous resins,and SP825 resin was chosen,followed by optimizing process *** optimum sample volume,feed co.centration,ratio of diameter to height,and feeding flow rate were 1.5 bed volumes(BV),15 mg/mL,1:10 and 1 BV/h,*** desorption was performed co.secutively with 8 BV of distilled water,3 BV of 5% ethanol and 8 BV of 10% ethanol at a flow rate of 2 BV/*** three cycles in separating 3.5 tons of rhodiola kirilowii roots,salidroside purity was increased from 3.4% in the crude extract to 93.6% in purified salidroside *** study provides a novel method to separate salidroside for injectable use.

关键词：红景天苷色谱分离大孔树脂树脂柱注射用心肌缺血治疗层析纯化

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：