Therapeutic Effects of Different Animal Bile Powders on Lipid Metabolism Disorders and Their Composition Analysis

作     者：CHEN Da-xin CHU Jian-feng LIN Shan ZHANG Ling CHEN Hong-wei SUN Zhi-wei XU Jian-feng CAI Qiao-yan WANG Li-li PENG Jun CHEN Da-xin;CHU Jian-feng;LIN Shan;ZHANG Ling;CHEN Hong-wei;SUN Zhi-wei;XU Jian-feng;CAI Qiao-yan;WANG Li-li;PENG Jun

作者机构：Academy of Integrative Medicine Cardiovascular Research InstituteFuzhou(350122)China Fujian Key Laboratory of Integrative Medicine on GeriatricFujian University of Traditional Chinese MedicineFuzhou(350122)China Chen Ke-ji Academic Thought Heritage StudioFuzhou(350122)China Fujian Guizhentang Pharmaceutical Co.Ltd.HuianFujian Province(362142)China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2022年第28卷第10期

页      面：918-923页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Developmental Fund of Chen Keji Integrative Medicine (No.CKJ2013009) Project-Oriented Collaboration with Fujian Guizhentang Pharmaceutical Co.,Ltd.(No.701141002) 

主　　题：bile powders lipid metabolism active ingredient liver 

摘      要：Objective: To compare the therapeutic effect of different animal bile powders on lipid metabolism disorders induced by high-fat diet in rats, and analyze the bioactive components of each animal bile powder. Methods: Sixty Sprague-Dawley rats were randomly divided into 6 groups(n=10): normal diet control group, high-fat diet model group, high-fat diet groups orally treated with bear, pig, cow and chicken bile powders, respectively. Serum biochemical markers from the abdominal aorta in each group were analyzed. Changes in the body weight and liver weight were recorded. Pathohistological changes in the livers were examined. High performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was used to determine the composition of bioactive components in each animal bile powder. Results: Treatment with different types of animal bile powders had different inhibitory effects on high-fat diet-induced increase of body weight and/or liver weight in rats, most notably in bear and pig bile powders(P0.05). High-fat diet induced lipid metabolism disorder in rats, which could be reversed by treatment with all kinds of bile powders. Bear bile and chicken bile showed the most potent therapeutic effect against lipid metabolism disorder. Cow and bear bile effectively alleviated high-fat diet induced liver enlargement and discoloration, hepatocyte swelling, infiltration of inflammatory cells and formation of lipid vacuoles. Bioactive component analysis revealed that there were significant differences in the relative content of taurocholic acid, taurodeoxycholic acid and ursodeoxycholic acid among different types of animal bile. Interestingly, a unique component with molecular weight of 496.2738 Da, whose function has not yet been reported, was identified only in bear bile powder. Conclusions: Different animal bile powders had varying therapeutic effect against lipid metabolism disorders induced by high-fat diet, and bear bile powder demonstrated th