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Journal of forestry Research 2018年第2期29卷 310-319页

作者： Bowen Chen Yufei Xiao Junji Li Hailong Liu Hu Chen Jie Jia Nan Chao Ying Gai Xiangning Jiang College of Biological Sciences and Biotechnology Beijing Forestry University Guangxi Key Laboratory of Superior timber trees Resource Cultivation Guangxi Forestry Research Institute

Geranylgeranyl pyrophosphate synthetase(GGPPS) has gained increasing attention as a key enzyme in terpene *** designed specific primers based on plant GGPPS homologs and used reverse transcription polymerase chain reaction(RT-PCR) to obtain and identify Pin GGPPS,a GGPPS gene sequence from Pinus massoniana,using bioinformatics *** PCR analysis of Pin GGPPS expression levels in roots,pine needles,immature stems,and semilignified stems from 6-month-old *** showed that expression levels of Pin GGPPS were highest in pine needles,followed by immature stems and semilignified stems,and lowest in *** we examined the correlation between Pin GGPPS gene expression levels and resin productivity in 20 adult plants for 28 successive days,Pin GGPPS expression levels presented a substantially linear distribution when plotted against their corresponding resin *** summary,we characterized the gene Pin GGPPS for the first time in ***,and established a correlation between Pin GGPPS gene expression levels and resin productivity,suggesting the importance of theory and production practice for ***.

关键词： Gene expression level GGPPS PinGGPPS Pinus massoniana Resin productivity

Transcript Accumulation Dynamics of Phenylpropanoid Pathway Genes in the Maturing Xylem and Phloem of Picea abies during Latewood Formation

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Journal of Integrative Plant Biology 2011年第10期53卷 783-799页

作者： Giovanni Emiliani Maria Laura Traversi Monica Anichini Guido Giachi Alessio Giovannelli trees and timber Institute-National Research Council department of Chemistry "U.Schiff" University of Florence

In temperate regions, latewood is produced when cambial activity declines with the approach of autumnal dormancy. The understanding of the temporal （cambium activity vs dormancy） and spatial （phloem, cambial region, maturing xylem） regulation of key genes involved in the phenylpropanoid pathway during latewood formation represents a crucial step towards providing new insights into the molecular basis of xylogenesis. In this study, the temporal pattern of transcript accumulation of 12 phenylpropanoid genes （PAL1, C4H315, C4H4, 4CL3, 4CL4, HCT1, C3H3, CCoAOMT1, COMT2, COMT5, CCR2） was analyzed in maturing xylem and phloem of Picea abies during latewood formation. Quantitative reverse transcription-polymerase chain reaction analyses revealed a well-defined RNA accumulation pattern of genes involved in the phenylpropanoid pathway during latewood formation. Differences in the RNA accumulation patterns were detected between the different tissue types analyzed. The results obtained here demonstrated that the molecular processes involved in monolignol biosynthesis are not restricted to the cambial activity timeframe but continued after the end of cambium cell proliferation. Furthermore, since it has been shown that lignification of maturing xylem takes place in late autumn, we argue on the basis of our data that phloem could play a key role in the monoliqnol biosynthesis process.

关键词： cambial region gene expression latewood formation xylogenesis.

Plantlet regeneration of adult Pinus massoniana Lamb. trees using explants collected in March and thidiazuron in culture medium

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Journal of forestry Research 2017年第6期28卷 1165-1171页

作者： Yin Wang Ruiling Yao Guangxi Key Laboratory of Superior timber trees Resource Cultivation Guangxi Forestry Research InstituteNanning 530002 Guangxi People’s Republic of China

A protocol for micropropagation using nodal explants from mature Pinus massoniana trees has been developed. Time of explant collection is crucial for the initial success of aseptic culture. Explants collected in early March gave the highest percentage of explant survival (64.5%) and shoot-forming percentage (52.3%). Thidiazuron (TDZ) concentration significantly influenced shoot formation; 4 mu M TDZ was optimum, with 4.8 shoots produced per explant with a mean length of 7.1 cm after 120 days of culture. Regenerated shoots rooted for 60 days in basic medium with 1 mu M NAA were ready for growth in pots. This is the first report on plantlet regeneration in vitro from mature trees of P. massoniana that provides a reliable method for propagating selected elites.

关键词： Thidiazuron Nodal segment Adventitious root Acclimatization NAA

Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch

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Phyton-International Journal of Experimental Botany 2021年第2期90卷 583-593页

作者： Kuipeng Li Hua Han Yunhui Xie Xiaomei Sun State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing100091China Guangxi Key Laboratory of Superior timber trees Resource Cultivation&Key Laboratory of Central South Fast-Growing timber Cultivation of forestry Ministry of China Guangxi Forestry Research InstituteNanning530002China

Optimization of in vitro adventitious root induction contributes to the development of a large-scale production system of hybrid clone seedlings of larch(Larix spp.).We used orthogonal testing to investigate the factors that affect the rooting of hybrid larch shoots-activated carbon,vitamin B1,sucrose,glycine,glutamic acid(Glu),cysteine,pH,and induction time in the dark and indole-3-butyric acid(IBA).Variance analysis showed that the effects of sucrose concentration on rooting rate,survival rate,and average root number,pH on the average number of roots;and Glu on survival rate were all significant(p<0.05)when compared to the mock *** all of the 45 treatments,the highest rooting rate was 90%,the highest survival rate was 100%,and the highest average root number was *** theoretically optimal procedure of in vitro root induction of hybrid larch shoots is shoot induction on L9 modified medium(pH 6.7)containing 2 mg.L^(-1)naphthalene acetic acid,0.25 mg.L^(-1)IBA,1/3 macroelements,7.5 g.L^(-1)sucrose,and 5 mg.L^(-1)Glu for 8 days,and then root induction on L9 modified medium without auxin.

关键词： Hybrid larch adventitious root orthogonal test variance analysis

Selection of superior families of Pinus massoniana in southern China for large-diameter construction timber

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Journal of forestry Research 2020年第2期31卷 475-484页

作者： Zhangqi Yang Hui Xia Jianhui Tan Yuanheng Feng Yongli Huang Guangxi forestry Research Institute Nanning 530002People’s Republic of China Key Laboratory of Central South Fast-Growing timber Cultivation of forestry Ministry of China Nanning 530002People’s Republic of China Guangxi Key Laboratory of Superior timber trees Resource Cultivation Nanning 530002People’s Republic of China Nanning forestry Research Institute Nanning 530107People’s Republic of China

This study addresses the increasing demand for large-diameter production timber,and considers the time and space variability of half-sib families of Pinus ***,diameter at breast height(DBH)and timber volume of 440 open-pollinated half-sib progeny families were investigated in 14 progeny trials in different years and production *** evaluation of the genetic variation of all half-sib families was carried out during the sustainable rapid growth period and individual volumes were characterized as a major *** analysis showed that there was considerable variance in the growth traits of most families in different years and on different *** variations caused by temporal and spatial changes of the mating system required three selection methods for *** results show that there were differences among the heritabilities of different growth traits by different halfsib *** heritability values of height,DBH and volume were 0.33,0.34 and 0.36,*** superior families were selected in every progeny test,12 were selected in progeny trials by different years and five in different habitat progeny *** superior families(Gui GC553A,Gui GC414A and Gui GC431A)were selected,although in different years and production *** genetic gains of timber volume of these selected r families ranged from 1.20 to 47.00%,which could provide a foundation for superior wood property selection and serve as material for seed improvement and extension in surrounding areas.

关键词： Pinus massoniana Open-pollinated progeny Large-diameter timber Superior family selection Mating system

Efficiency of Bio-Fertilizing as One of the Natural Alternatives to Improve the Growth of Khaya senegalensis and Swietenia mahagoni trees and for Sustainability

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Agricultural Sciences 2024年第2期15卷 292-310页

作者： Safaa A. S. Ghorab Nashwa H. Mohamed Nader A. El-Shanhorey Nabal S. El-Shahat Amr R. Rabie forestry and timber trees department Horticulture Research Institute Agricultural Research Center Ismailia Egypt forestry and timber trees department Horticulture Research Institute Agricultural Research Center Alexandria Egypt Botanical Gardens department Horticulture Research Institute Agricultural Research Center Alexandria Egypt forestry and timber trees department Horticulture Research Institute Agricultural Research Center Giza Egypt

A field experiment was carried out at Ismailia Research Station, Ismailia Governorate from 2020-2022 to improve the growth of Khaya senegalensis and Swietenia mahagoni by using a combination of mineral fertilizer (NPK) and biological fertilizer (Azotobacter chroococcum, Bacillus megatherium, and Bacillus circulant) as recommended dose under new sandy soils conditions. Split plot designed with four treatments (Control, (50% Mineral fertilizer (M.) + 50% Biological fertilizer (Bio.)), 100% M. and 100% Bio.) of each species. Vegetative growth, leaf area, tree biomass, stored carbon, basal area, tree volume, and in the soil both of microbial account and mineral content were determined. The experimental results showed no significant differences between studied species among the most studied parameters except for Khaya senegalensis which gave the highest significant difference in root biomass and below-stored carbon than Swietenia mahagoni. Evidently, the highest significant growth parameters were 100% mineral fertilizer followed by (50% M. + 50% Bio.) as compared with control. No significant difference between 100% M. and (50% M. + 50% Bio.) of shoot dry biomass (15.19 and 12.02 kg, respectively) and above-stored carbon (0.28 and 0.22 Mt, respectively). Microbial account and mineral content in soil were improved after cultivation of tree species compared to before planting and control, especially with 50% mineral fertilizer and 50% bio-fertilizer treatment. In conclusion, a treatment containing 50% mineral fertilizer and 50% bio-fertilizer has led to the ideal Khaya senegalensis and Swietenia mahagoni growth in sandy soil for cheaper and sustainable.

关键词： Khaya senegalensis Swietenia mahagoni Mineral Fertilizer Bio-Fertilizer Growth Parameters Tree Biomass Stored Carbon Sustainable

Phylogenetic relationships among five species of Armeniaca Scop. (Rosaceae) using microsatellites (SSRs) and capillary electrophoresis

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Journal of forestry Research 2016年第5期27卷 1077-1083页

作者： Dali Fu Lüyi Ma Yue Qin Mengpei Liu Han Zhao Gaopu Zhu Hao Fu College of forestry Beijing Forestry UniversityBeijing 100083 China Non-timber forestry Research and Development Center Chinese Academy of Forestry Zhengzhou 450003 China College of forestry Central South University of Forestry and Technology Changsha 410004 China

The genus Armeniaca Scop. is well known for its popular cultivated edible fruit trees such as Armeniaca vul- garis Lam. and ornamental flowers such as A. mume Sieb. Another species, A. cathayana Fu et al., one of six important dry fruits （kernel-using apricot）, is cultivated for its edible seeds in North China. In the present study, DNA from 70 individuals of A rmeniaca, including 38 of A. cathayana, 18 ofA. vulgaris, 12 ofA. sibirica, 1 ofA. dasycarpa and 1 of A. mume, was extracted and analyzed using microsatellites and capillary electrophoresis. For 20 polymorphic loci selected, 339 alleles and 140.7 effective alleles were detected. The number of alleles per locus ranged from 8 to 28, with an average of 16.95 alleles per locus. The observed heterozygosity （Ho） and the expected heterozygosity （He） ranged from 0.427 to 0.971 and from 0.737 to 0.912, respectively. The polymorphism information content varied from 0.708 to0.905, with an average of 0.827. Based on the genetic similarity among 70 individuals, a UPGMA was used to establish the phylogenetic relationships. The taxonomic positions among five species were clearly revealed, and A. cathayana was more closely related to A. vulgaris than to A. sibirica. The results will provide a scientific basis for research on the taxonomy, germplasm resources and breeding ofArmeniaca, especially for A. cathayana.

关键词： Armeniaca Scop. - Armeniaca cathayana Microsatellite Capillary electrophoresis Phylogenetic relationship Polymorphism Simple sequence repeat

Effects of IBA on the rooting of branch cuttings of Chinese jujube (Zizyphus jujuba Mill.) and changes to nutrients and endogenous hormones

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Journal of forestry Research 2018年第6期29卷 1557-1567页

作者： Fengxia Shao Sen Wang Wen Huang Zhiming Liu Key Laboratory of Cultivation and Protection for Non-Wood Forest trees Key Laboratory of Non-wood Forest Products of State Forestry AdministrationCentral South University of Forestry and Technology department of Biology Eastern New Mexico University

‘Zhongqiusucui’ jujube secondary shoots were treated with 3-indolebutyric acid(IBA) at three concentrations, 500, 1000 and 1500 mg/L. Results show that IBA could significantly enhance rooting and root characteristics of cuttings and were best with IBA at 1500 mg/L. In the rooting process, the formation of adventitious roots was related to the consumption and accumulation of nutrients(soluble sugars and proteins) and the changes in endogenous hormones in phloem, leaf tips and leaf bases. The rooting of cuttings had a positive correlation with the consumption of soluble sugars during the period of callus formation and with the accumulation of soluble sugars during adventitious root formation and growth. Rooting was positively related to the breakdown of soluble proteins in the phloem when the callus formed, and had a positive correlation with its accumulation during adventitious root formation and growth. Leaf tips and leaf bases showed a reverse trend in changes of soluble protein. However, together with the phloem, leaf tips and leaf bases regulated and controlled the formation and development of adventitious roots. The main activities of soluble proteins exist in the leaf tips as this was the main source of soluble proteins. The relation between rooting and IAA(indole-3-acetic acid) content in phloem was positive and thus a high concentration of IAA could benefit the induction and formation of adventitious roots. However, rooting was negatively related with ABA(abscisic acid) and GA(gibberellic acid) and a high concentration of both could inhibit the induction and formation of adventitious roots. Rooting had a positive correlation with phloem IAA/ABA ratios, and higher ratios could improve rooting. Low concentrations of ZR(zeatin riboside) triggered the induction of adventitious roots, while higher concentrations promoted root *** hormones in leaf tips and bases had an impact on rooting. The activities of endogenous hormones mainly existed in leaf tip

关键词： Chinese jujube Ziziphus jujuba Mill. Rooting of cuttings Soluble sugars Soluble proteins Endogenous hormones

Progress in the Study of Molecular Genetic Improvements of Poplar in China

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Journal of Integrative Plant Biology 2006年第9期48卷 1001-1007页

作者： Shan-Zhi Lin Zhi-Yi Zhang Qian Zhang Yuan-Zhen Lin Key Laboratory for Genetics and Breeding in Forest trees and Ornamental Plants of Ministry of Education Beijing forestry University Beijing 100083 China

The poplar is one of the most economically important and intensively studied tree species owing to its wide application in the timber industry and as a model material for the study of woody plants. The natural resource of poplars in China is replete. Over the past 10 years, the application of molecular biological techniques to genetic improvements in poplar species has been widely studied in China. Recent advances in molecular genetic improvements of poplar, including cDNA library construction, gene cloning and identification, genetic engineering, gene expression, genetic linkage map construction, mapping of quantitative trait loci （QTL） and molecular-assisted selection, are reviewed in the present paper. In addition, the application of modern biotechnology to molecular improvements in the genetic traits of the poplar and some unsolved problems are discussed.

关键词： poplar gene engineering genetic improvement genetic linkage maps molecular marker quantitative trait loci (QTL) mapping

Characteristics of rock fragments in different forest stony soil and its relationship with macropore characteristics in mountain area, northern China

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Journal of Mountain Science 2018年第3期15卷 519-531页

作者： MENG Chen NIU Jian-zhi YIN Zheng-cong LUO Zi-teng LIN Xing-na JIA Jing-wei College of Soil and Water Conservation Beijing Forestry University Beijing Collaborative Innovation Center for Eco-environmental Improvement with forestry and Fruit trees Beijing University of Agriculture department of Geography Texas A&M University College Station

Rock fragments have major effect on soil macropores and water movement. However, the characteristics of rock fragments and their relationship with macropore characteristics remain elusive in forest stony soils in northern mountainous area of China. The objectives of this study are to(1) use Industrial Computed Tomography(CT) scanning to quantitatively analyze rock fragment characteristics in intact soil columns in different forest lands and(2) identify the relationship between characteristics of rock fragments and that of the macropores. Intact soil columns that were 100 mm in diameter and 300 mm long were randomly taken from six local forest stony soils in Wuzuolou Forest Station in Miyun, Beijing. Industrial CT was used to scan all soil column samples, and then the scanned images were utilized to obtain the three-dimensional(3 D) images of rock fragments and macropore structures. Next, theparameters of the rock fragments and macropore structure were measured, including the volume, diameter, surface area, and number of rock fragments, as well as the volume, diameter, surface area, length, angle, tortuosity and number of macropores. The results showed that no significant difference was found in soil rock fragments content in the 10-30 cm layer between mixed forest and pure forest, but in the 0-10 cm soil layer, the rock fragments in mixed forest were significantly less than in pure forest. The number density of macropores has significant negative correlation with the number of rock fragments in the 0-10 cm soil layer, whereas this correlation is not significant in 10-20 cm and 20-30 cm soil layers. The volume density of macropore was not correlated with the volume density of rock fragments, and there is no correlation between the density of macropore surface area and the density of rock fragment surface area. Industrial CT scanning combined with image processing technology canprovide a better way to explore 3 D distribution of rock fragments in soil. The content of

关键词： Rock fragment Macropore Forest stone soil Mountain area Industrial Computed Tomography