Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch

作     者：Kuipeng Li Hua Han Yunhui Xie Xiaomei Sun 

作者机构：State Key Laboratory of Tree Genetics and BreedingKey Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing100091China Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation&Key Laboratory of Central South Fast-Growing Timber Cultivation of Forestry Ministry of ChinaGuangxi Forestry Research InstituteNanning530002China 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)

年 卷 期：2021年第90卷第2期

页      面：583-593页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：This research was funded by The National Key Research and Development Program of China(Project No.2017YFD0600401) 

主　　题：Hybrid larch adventitious root orthogonal test variance analysis 

摘      要：Optimization of in vitro adventitious root induction contributes to the development of a large-scale production system of hybrid clone seedlings of larch(Larix spp.).We used orthogonal testing to investigate the factors that affect the rooting of hybrid larch shoots-activated carbon,vitamin B1,sucrose,glycine,glutamic acid(Glu),cysteine,pH,and induction time in the dark and indole-3-butyric acid(IBA).Variance analysis showed that the effects of sucrose concentration on rooting rate,survival rate,and average root number,pH on the average number of roots;and Glu on survival rate were all significant(p0.05)when compared to the mock *** all of the 45 treatments,the highest rooting rate was 90%,the highest survival rate was 100%,and the highest average root number was *** theoretically optimal procedure of in vitro root induction of hybrid larch shoots is shoot induction on L9 modified medium(pH 6.7)containing 2 mg.L^(-1)naphthalene acetic acid,0.25 mg.L^(-1)IBA,1/3 macroelements,7.5 g.L^(-1)sucrose,and 5 mg.L^(-1)Glu for 8 days,and then root induction on L9 modified medium without auxin.