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Feeding behavior and hormoligosis associated with imidacloprid resistance in Asian citrus psyllid,Diaphorina citri

Insect Science 2024年第4期31卷 1211-1221页

作者： Xue Dong Chen Justin George Lauren M.Diepenbrock Hunter Gossett Guoping Liu Jawwad A.Qureshi Lukasz L.Stelinski entomology and Nematology Department University of FloridaCitrus Research and Education CenterLake AlfredFloridaUSA Southern Insect Management Research Unit USDA-ARSStonevilleMississippiUSA entomology and Nematology Department University of FloridaSouthwest Florida Research and Education CenterImmokaleeFloridaUSA

Imidacloprid is a neonicotinoid insecticide used for managing the Asian citrus psyllid,Diaphorina citri Kuwayama,which serves as vector of phytopathogens causing citrus ***,development of resistance to neonicotinoids among populations of *** has coincided with occasional control failures in the *** objectives of this research were to(1)survey current levels of imidacloprid resistance in florida citrus;(2)compare feeding behavior between imidacloprid-resistant and susceptible *** using electrical penetration graph recordings,and(3)investigate the possible amplification of insecticide hormoligosis associated with *** surveys confirmed that the susceptibility of *** populations to imidacloprid has decreased in commercial florida citrus groves compared with a laboratory-susceptible *** 12 generations of selection,resistance to imidacloprid increased by 438 fold compared with the susceptible ***-susceptible *** feeding on citrus exhibited significantly more bouts associated with intercellular pathway(C),phloem penetration(D),phloem salivation(E1),and nonprobing(Np)activities than imidacloprid-resistant ***,there were no differences observed in the frequency or duration of phloem ingestion or xylem feeding between susceptible and resistant *** was no statistical difference in fecundity between resistant and susceptible ***,the fecundity of imidacloprid-susceptible female *** treated with a sublethal concentration of imidacloprid(LC_(25))increased significantly compared with controls,while such hormoligosis was less pronounced among imidacloprid-resistant *** results suggest that imidacloprid-resistant psyllids may cease feeding sooner than susceptible counterparts following sublethal exposure to this insecticide,indicative of a behavioral resistance mechanism.

关键词： artificial selection electrical penetration graph feeding behavior hormoligosis insecticide resistance sublethal exposure

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Synergistic effects of the entomopathogenic fungus Isaria javanica and low doses of dinotefuran on the efficient control of the rice pest Sogatella furcifera

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Journal of Integrative Agriculture 2024年第2期23卷 621-638页

作者： Tingting Zhou Qian Zhao Chengzhou Li Lu Ye Yanfang Li Nemat OKeyhani Zhen Huang Department of entomology College of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China Guangdong Provincial Key laboratory of High Technology for Plant Protection Plant Protection Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510642China Department of Microbiology and Cell Science Institute of Food and Agricultural SciencesUniversity of FloridaFL 32611USA

The rice planthopper,Sogatella furcifera,is a piercing-sucking insect pest of rice,Oryza *** is responsible for significant crop yield losses,and has developed moderate to high resistance to several commonly used chemical *** investigated the effects of the insect fungal pathogen Isaria javanica,alone and in combination with the chemical insecticide dinotefuran,on *** under both laboratory and field *** results show that *** displays high infection efficiency and mortality for different stages of ***,reducing adult survival,female oviposition and ovary *** bioassays showed that the combined use of *** with a low dose(4-16 mg L^(-1))of dinotefuran resulted in higher mortality in *** than the use of *** or dinotefuran *** combined treatment also had more significant effects on several host enzymes,including superoxide dismutase,catalase,peroxidase,and prophenol oxidase *** field trials,*** effectively suppressed populations of rice planthoppers to low levels(22-64%of the level in untreated plots).Additional field experiments showed synergistic effects,i.e.,enhanced efficiency,for the control of *** populations using the combination of a low dose of ***(1×10^(4) conidia mL^(-1))and a low dose of dinotefuran(~4.8-19.2%of normal field use levels),with control effects of>90%and a population level under 50 insects per 100 hills at 3-14 days *** findings indicate that the entomogenous fungus *** offers an attractive biological control addition as part of the integrated pest management(IPM)practices for the control of rice plant pests.

关键词： Isaria javanica Sogatella furcifera entomopathogenic fungus white planthopper rice synergistic effect on pest control

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A single degenerated primer significantly improves COX1 barcoding performance in soil nematode community profiling

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Soil Ecology Letters 2024年第2期6卷 31-44页

作者： Yincai Ren Dorota L. Porazinska Quanhui Ma Shuhan Liu Hongmei Li Xue Qing Department of Plant Pathology Nanjing Agricultural UniversityNanjing210095China Department of entomology and Nematology University of FloridaGainesvilleFL32611USA Institute of Grassland Science Key Laboratory of Vegetation Ecology of the Ministry of EducationJilin Songnen Grassland Ecosystem National Observation and Research StationNortheast Normal UniversityChangchun130024China

A new COX1 primer for soil nematode metabarcoding was designed,and this primer outperforms other commonly used COX1 primer pairs in species recovery and quantity of PCR products.•The lack of reference database is the main reason that led to the low species recovery in COX1 metabarcoding.•We expanded current NCBI database by adding 51 newly generated COX1 reference *** nematodes play important roles in soil ecosystems and often serve as bioindicators of soil *** identification of soil nematodes is often difficult due to their limited diagnostic characters and high phenotypic *** barcoding and metabarcoding techniques are promising but lack universal primers,especially for mitochondrial COX1 *** this study a degenerated COX1 forward primer COIFGED was *** primer pair(COIFGED/JB5GED)outperforms other four commonly used COX1 primer pairs in species recovery and quantity of polymerase chain reaction(PCR)*** metabarcoding analysis,the reads obtained from the new primer pair had the highest sequencing saturation threshold and amplicon sequence variant(ASV)diversity in comparison to other COX1 as well as 18S rRNA *** annotation of ASVs suggested the new primer pair initially recovered 9 and 6 out of 25 genera from mock communities,respectively,outperformed other COX1 primers,but underperformed the widely used 18S NF1/18Sr2b primers(16 out of 25 genera).By supplementing the COX1 database with our reference sequences,we recovered an additional 6 mock community species bringing the tally closer to that obtained with 18S *** summary,our newly designed COX1 primers significantly improved species recovery and thus can be supplementary or alternative to the conventional 18S metabarcoding.

关键词： degenerated primers DNA metabarcoding mitochondrial cytochrome oxidase c subunit I gene phylogeny ribosomal RNA gene soil nematodes

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Satyrization and satyrization-resistance in competitive displacements of invasive mosquito species

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Insect Science 2016年第2期23卷 162-174页

作者： Irka Ewa Bargielowski Leon Philip Lounibos florida medical entomology laboratory University of Florida Vero Beach Florida USA

Competitive displacements or reductions of resident populations of insects, often effected by a related species, may be caused by a variety of mechanisms. Satyrization is a form of mating interference in which males of one species mate with females of another species, significantly decreasing their fitness and not generating hybrids. Satyriza- tion has been established to be the probable cause of competitive displacements of resident mosquitoes by invasive species, especially of Aedes aegypti by Aedes albopictus, two important vectors of dengue and chikungunya viruses. Mathematical models predict that even low levels of asymmetric mating interference are capable of producing competitive displacements or reductions. Couplings of virgin Ae. aegypti females with Ae. albopictus males effectively sterilize these females through the monogamizing actions of male ac- cessory gland products, but the converse interspecific mating does not impact the future reproduction ofAe. albopictus females. Populations ofAe. aegypti exposed to satyrization quickly evolve resistance to interspecific mating, which is believed to ameliorate repro- ductive interference from, and promote co-existence with, Ae. albopictus. The evolution of satyrization resistance among Ae. aegypti in laboratory cages is accompanied by fitness costs, such as reduced fecundity and slower receptivity to conspecific males. Cage exper- iments and field observations indicate that Ae. albopictus males are capable of satyrizing females of other species of the Stegomvia subgenus, potentially leading to competitive displacements, and possible extinctions, especially of endemic species on islands. Exam- ination of other examples of reproductive interference in insects reveals few parallels to the mechanism and outcomes of satyrization by Ae. albopictus. We conclude by posing the hypothesis that satyrization may favor the ecological success of Ae. albopictus, and suggest many lines for future research on this phenomenon.

关键词： Aedes aegypti Aedes albopictus mating interference reproductive interfer-ence satyrization

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Cold acclimation increases Asian citrus psyllid Diaphorina citri (Hemiptera: Liviidae) survival during exposure to freezing temperatures

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Insect Science 2022年第2期29卷 531-538页

作者： Xavier Martini Kathi Malfa Dara Stockton Monique JRivera Department of entomology and Nematology North Florida Research and Education CenterQuincyFloridaUSA USDA-ARS U.S.Pacific Basin Agricultural Research CenterHiloHawaiiUSA Department of entomology University of CaliforniaRiversideCaliforniaUSA

The Asian citrus psyllid,Diaphorina citri,is the vector of the pathogenic bacteria that causes Huanglongbing,the most devasting disease affecting citrus *** cultivation of citrus tends to expand northward,the tolerance of psyllids to freezing temperatures needs to be *** mortality of *** to cold temperature has been previously studied,much less is known regarding the acclimation potential of psyllids to cold *** first evaluated cold resistance of *** depending on color morph and ***,we compared mortality of *** at−4°C for 10 hours between unacclimated psyllids,psyllids that were gradually acclimated to cold over 1 or 2 weeks,and psyllids intermittently acclimated also for 1 or 2 *** did not find major differences in cold resistance between color morphs and *** found that after 1 week of gradual acclimation,the survival of psyllids at−4°C increased to 86%,compared to 20%in the control *** did not increase after the second week of gradual *** intermittent acclimation improved survival compared to the control group,it was less effective than gradual acclimation with a survival at 30%and 70%after 1 and 2 weeks of acclimation,respectively,although this difference with gradual acclimation was not significant at week *** data show that gradual cold acclimation allows *** to survive brief periods below *** may serve as a mechanism responsible for increasing northern establishment of this pest.

关键词： cold acclimation cold hardy citrus cold resistance citrus greening Diaphorina citri overwintering

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Anopheles gambiae strain(Ag55)cultured cells originated from Anopheles coluzzii and are phagocytic with hemocyte-like gene expression

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Insect Science 2022年第5期29卷 1346-1360页

作者： Ruchir Mishra Gang Hua Ujwal R.Bagal Donald E.Champagne Michael J.Adang Department of entomology College of Agricultural and Environmental SciencesUniversity of GeorgiaAthensGeorgiaUSA Department of entomology and Nematology University of FloridaGainesvilleFloridaUSA Institute of Bioinformatics University of GeorgiaAthensGeorgiaUSA Department of Biochemistry and Molecular Biology University of GeorgiaAthensGeorgiaUSA

Anopheles gambiae and Anopheles coluzzii are closely related species that are predominant vectors of malaria in ***,*** form M was renamed *** and we now conclude on the basis of a diagnostic PCR-restriction fragment length polymorphism assay that Ag55 cells were derived from *** established an Ag55 cell transcriptome,and KEGG pathway analysis showed that Ag55 cells are enriched in phagosome pathway *** Ag55 transcriptome has an abundance of specific transcripts characteristic of mosquito *** *** bioparticle uptake experiments visualized by fluorescence microscopy and confocal microscopy and quantified by flow cytometry establish the phagocytic competence of Ag55 *** from this investigation of Ag55 cell properties will guide researchers in the use and engineering of the Ag55 cell line to better enable investigations of Plasmodium,other microbes,and insecticidal toxins.

关键词： Anopheles coluzzii transcriptome Anopheles gambiae confocal microscopy flow-cytometry FPKM hemocyte-like phagocytosis

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Characterization of the voltage-gated sodium channel of the Asian citrus psyllid, Diaphorina citri

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Insect Science 2017年第1期24卷 47-59页

作者： Bin Liu Monique R. Coy Jin-Jun Wang Lukasz L. Stelinski entomology and Nematology Department Citrus Research and Education Center University of Florida Florida USA Key laboratory of entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing China

The Asian citrus psyllid, Diaphorina citri Kuwayama （Hemiptera： Liviidae）, is an important insect pest of citrus. It is the vector of ＇Candidatus＇ Liberibacter asiaticus, a phloem-limited bacterium that infects citrus, resulting in the disease Huanglongbing （HLB）. Disease management relies heavily on suppression of D. cirri populations with insecticides, including pyrethroids. In recent annual surveys to monitor insecticide resistance, reduced susceptibility to fenpropathrin was identified in several field populations olD. citri. The primary target ofpyrethroids is the voltage-gated sodium channel （VGSC）. The VGSC is prone to target-site insensitivity because of mutations that either reduce pyrethroid binding and/or alter gating kinetics. These mutations, known as knockdown resistance or kdr, have been reported in a wide diversity of arthropod species. Alternative splicing, in combination with kdr mutations, has been also associated with reduced pyrethroid efficacy. Here we report the molecular characterization of the VGSC in D. citri along with a survey of alternative splicing across developmental stages of this species. Previous studies demonstrated that D. citri has an exquisite enzymatic arsenal to detoxify insecticides resulting in reduced efficacy. The results from the current investigation demonstrate that target-site insensitivity is also a potential basis for insecticide resistance to pyrethroids in D. citri. The VGSC sequence and its molecular characterization should facilitate early elucidation of the underlying cause of an established case of resistance to pyrethroids. This is the first characterization of a VGSC from a hemipteran to this level of detail, with the majority of the previous studies on dipterans and lepidopterans.

关键词： citrusgreening diseasemanagement knockdownresistance(kdr) target-site insensitivity

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Potential of termite-based biomass pre-treatment strategies for use in bioethanol production

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Insect Science 2010年第3期17卷 166-174页

作者： Michael E. Scharf Drion G. Boucias entomology and Nematology Department University of Florida Gainesville Florida USA

When considering the current state of the biorefinery industry, it is readily apparent that industrial cellulose and hemicellulose digestion processes are relatively advanced, whereas enzymatic pre-treatment strategies for biomass delignification and cellulose solubilization are not well developed. The need for efficient biomass pre-treatment strategies presents a significant opportunity for researchers studying lignocellulose digestion in termites and other insects. With an emphasis on industrial biomass pre-treatment, this review provides an overview of：（i） industrial biorefining operations （feedstocks, processing, and economics）; （ii） recent findings from termite research that have revealed candidate enzymes; and （iii） research needs and opportunities for consideration by entomologists working in this area. With respect to research findings, recently identified candidate lignases （laccases, catalases, peroxidases, esterases）, other potentially important detoxification enzymes （cytochrome P450, superoxide dismutase）, and phenolic acid esterases （carboxylesterases） that may assist in hemicellulose solubilization are overviewed. Regarding research needs and opportunities, several approaches for identification of candidate pre-treatment enzymes from upstream, symbiont-free gut regions are also described.

关键词： bioenergy bioethanol biorefining Isoptera lignocellulose renewable energy

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Transcytosis of Junonia coenia densovirus VP4 across the gut epithelium of Spodoptera frugiperda(Lepidoptera:Noctuidae)

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Insect Science 2020年第1期27卷 22-32页

作者： Mariah Kemmerer Bryony C.Bonning Department of entomology Iowa State UniversityAmesIowaUSA Department of entomology and Nematology University of FloridaGainesvilleFloridaUSA

The Junonia coenia densovirus rapidly traverses the gut epithelium of the host lepidopteran without replicating in the gut *** ability of this virus to transcytose across the gut epithelium is of interest for the potential use of virus structural proteins as delivery vehicles for insecticidal peptides that act within the insect hemocoel,rather than in the *** this study,we used fall armyworm,Spodoptera frugiperda to examine the binding of the virus to brush border membrane vesicle proteins by two-dimensional ligand blot *** also assessed the rate of flux of the primary viral structural protein,VP4 fused to eGFP with a proline-rich linker(VP4-P-eGFP)through the gut epithelium ex vivo in an Ussing *** mechanisms involved with transcytosis of VP4-P-eGFP were assessed by use of *** serum albumin(BSA)and eGFP were used as positive and negative control proteins,*** contrast to BSA,which binds to multiple proteins on the brush border membrane,VP4-P-eGFP binding was specific to a protein of high molecular *** flux was significantly higher for VP4-P-eGFP after 2 h than for albumin or eGFP,with rapid transcytosis of VP4-P-eGFP within the first 30 *** contrast to BSA which transcytosed following clathrin-mediated endocytosis,the movement of VP4-P-eGFP was vesicle-mediated but *** specificity of binding combined with the efficiency of transport across the gut epithelium suggest that VP4 will provide a useful carrier for insecticidal peptides active within the hemocoel of key lepidopteran pests including ***.

关键词： BSA gut epithelium JcDNV Lepidoptera transcytosis virus

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Botanicals, selective insecticides, and predators to control Diaphorina citri （Hemiptera： Liviidae） in citrus orchards

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Insect Science 2014年第6期21卷 717-726页

作者： Azhar A. Khan Muhammad Afzal Jawwad A. Qureshi Arif M. Khan Abubakar M. Raza Department of Agricultural entomology University of Sargodha Sargodha Pakistan Department of entomology & Nematology Southwest Florida Research and Education Center Institute of Food and Agricultural Sciences University of Florida Immokalee Florida USA Department of Biotechnology University of Sargodha Sargodha Pakistan

The Asian citrus psyllid （ACP） Diaphorina citri Kuwayama vectors pathogens that cause huanglongbing （HLB） or citrus greening devastating and economically im- portant disease present in most citrus growing regions. Young citrus shoots are required for psyllid reproduction and development. During winter citrus trees produce little or no new growth. Overwintering adults reproduce in spring on newly emerging shoots also attractive to other pests and beneficial insects. Botanicals and relatively selective insecti- cides could help to conserve beneficial insects and reduce pest resistance to insecticides. Sprays of Azadirachtin （Neem）, Tropane （Datura）, Spirotetramat, Spinetoram, and broad- spectrum Imidacloprid were evaluated to control ACP in spring and summer on 10-year-old ＂Kinow＂ Citrus reticulata Blanco trees producing new growth. Psyllid populations were high averaging 5-9 nymphs or adults per sample before treatment application. Nymphs or adults were significantly reduced to 0.5-1.5 per sample in all treatments for 3 weeks, aver- age 61%-83% reduction. No significant reduction in ladybeetles Adalia bipunctata, Ane- glei scardoni, Cheilomenes sexmaculata, and Coccinella septempunctata was observed. Syrphids, spiders and green lacewings were reduced in treated trees except with Tropane. Studies are warranted to assess impact of these predators on ACP and interaction with insecticides. Observed reduction in ACP populations may not be enough considering its reproductive potential and role in the spread of HLB. Follow-up sprays may be required to achieve additional suppression using rotations of different insecticides.

关键词： Asian citrus psyllid biopesticides Coccinellids huanglongbing predators

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