Characterization of the voltage-gated sodium channel of the Asian citrus psyllid, Diaphorina citri

作     者：Bin Liu Monique R. Coy Jin-Jun Wang Lukasz L. Stelinski 

作者机构：Entomology and Nematology Department Citrus Research and Education Center University of Florida Florida USA Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing China 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2017年第24卷第1期

页      面：47-59页

核心收录：

学科分类：0710[理学-生物学] 090403[农学-农药学(可授农学、理学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：funded by a grant from the Citrus Research Development Foundation 

主　　题：citrusgreening diseasemanagement knockdownresistance（kdr） target-site insensitivity 

摘      要：The Asian citrus psyllid, Diaphorina citri Kuwayama （Hemiptera： Liviidae）, is an important insect pest of citrus. It is the vector of ＇Candidatus＇ Liberibacter asiaticus, a phloem-limited bacterium that infects citrus, resulting in the disease Huanglongbing （HLB）. Disease management relies heavily on suppression of D. cirri populations with insecticides, including pyrethroids. In recent annual surveys to monitor insecticide resistance, reduced susceptibility to fenpropathrin was identified in several field populations olD. citri. The primary target ofpyrethroids is the voltage-gated sodium channel （VGSC）. The VGSC is prone to target-site insensitivity because of mutations that either reduce pyrethroid binding and/or alter gating kinetics. These mutations, known as knockdown resistance or kdr, have been reported in a wide diversity of arthropod species. Alternative splicing, in combination with kdr mutations, has been also associated with reduced pyrethroid efficacy. Here we report the molecular characterization of the VGSC in D. citri along with a survey of alternative splicing across developmental stages of this species. Previous studies demonstrated that D. citri has an exquisite enzymatic arsenal to detoxify insecticides resulting in reduced efficacy. The results from the current investigation demonstrate that target-site insensitivity is also a potential basis for insecticide resistance to pyrethroids in D. citri. The VGSC sequence and its molecular characterization should facilitate early elucidation of the underlying cause of an established case of resistance to pyrethroids. This is the first characterization of a VGSC from a hemipteran to this level of detail, with the majority of the previous studies on dipterans and lepidopterans.