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Agricultural Sciences in China 2010年第8期9卷 1140-1149页

作者： Elya Masya Mohd Fishal Sariah Meon Wong Mui Yun laboratory of Food Crops and floriculture Institute of Tropical AgricultureUniversiti Putra Malaysia

This study is aimed at assessing the ability of two endophytic bacteria originally isolated from healthy oil palm roots, Pseudomonas sp. （UPMP3） and Burkholderia sp. （UPMB3） to induce resistance in susceptible Berangan banana against Fusarium oxysporum race 4 （FocR4）. Increased accumulation of resistance-related enzymes such as peroxidase （PO）, phenylalanine ammonia lyase （PAL）, lignithioglycolic acid （LTGA）, and pathogenesis-related （PR） proteins （ehitinase and β-1,3-glucanase） has been observed in plantlets treated with endophytic bacteria UPMP3 and UPMB3 singly or as mixture under glasshouse conditions. Pre-inoculation of banana plantlets with UPMP3 showed a significant reduction in Fusarium wilt incidence 72 d after challenged inoculation with FocR4. UPMB3 was less effective in suppressing Fusarium wilt compared to UPMP3, whereas, the mixture of both endophytes showed an intermediate effect. Based on these results, it is concluded that UPMP3 could be a promising biological control agent that can trigger resistance against Fusarium wilt in susceptible Berangan banana.

关键词： banana Pseudomonas sp. Burkholderia sp. induce resistance Fusarium wilt

Effect of Seed Bacterization on Plant Growth Response and Induction of Disease Resistance in Chilli

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Agricultural Sciences in China 2009年第8期8卷 963-971页

作者： Yasmeen Siddiqui Sariah Meon laboratory of Food Crops and floriculture Institute of Tropical AgricultureUniversiti Putra Malaysia (UPM)

This study aimed to examine the induction of disease resistance, and growth response in chilli plants elicited by plant growth promoting endophytic bacteria [Pseudomonas aeruginosa （UPMP3）, Burkholderia cepacia （UPMB3）, and Serratia marcescens （UPMS3）]. Seed bacterization with UPMP3 and UPMB3 significantly increased peroxidase （PO）, polyphenol oxidase （PPO）, and phenylalanine ammonia-lyase （PAL） activities. This increase corresponded to greater reduction in pre-and post-emergence damping-off caused by Sclerotium rolfsii. UPMS3 alone or as mixture with UPMP3 and UPMB3 did not show any significant reduction in disease incidence. However, all the isolates tested did not inhibit the seed germination and seedling establishment in chilli.

关键词： endophytic bacteria induced resistance PO PPO PAL Sclerotium rolfsii

Intron-retained alternatively spliced VIN3-LIKE 2 gene from Chimonanthus praecox promotes flowering in transgenic Arabidopsis

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Horticultural Plant Journal 2023年第4期9卷 789-800页

作者： Tian Xu Ning Liu Chunyu Wu Yi Zhang Mingkang Tian Shunzhao Sui Zhineng Li Key laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education) Chongqing Engineering Research Center for FloricultureCollege of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China

Vernalization is a process of acquiring or accelerating the flowering ability by prolonged cold *** INSENSITIVE3(VIN3)is induced by chilling and is extremely important for the vernalization response in Arabidopsis ***,the issue of the induction of the VIN3-LIKE genes in wintersweet(Chimonanthus praecox)has been largely *** the present study,we explored the molecular regulation of the PHD type finger protein-encoding gene CpVIL2 in relation to the growth and development of wintersweet in *** wintersweet,quantitative real-time PCR(qRT-PCR)analysis showed that the relative expression of CpVIL2-As2i(intron-retained alternatively spliced in the second intron)was extremely higher in the pistils than in the other *** the relative CpVIL2-As2i expression in flower buds(FBs)treated at 8°C was higher than that of FBs in December,2016 under natural conditions,which was not detected in non-flowering FBs at 16°*** Arabidopsis,the expression patterns of the CpVIL2-As2i gene were detected at first in CpVIL2-As2i pro::GUS(β-glucuronidase)lines,with predominantly higher expression in flowers and ***,the hormone-induced expression profiles of the CpVIL2-As2i promoter were confirmed using exogenous induction by abscisic acid(ABA)and indole acetic acid(IAA)phytohormones,where the GUS enzyme activity obviously decreased compared with that of *** comparison with Arabidopsis/Col-0,early flowering was detected in ectopic 35S::CpVIL2-As2i ***,these results demonstrated the function of the CpVIL2-As2i gene,at the same time,provided us with new insights into the molecular mechanisms of early flowering and complex regulatory networks of vernalization in wintersweet.

关键词： Chimonanthus praecox PHD finger protein VIL2 Ectopic expression Flowering time Vernalization

Integrated transcriptome and proteome analysis provides insight into chilling-induced dormancy breaking in Chimonanthus praecox

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Horticulture Research 2020年第1期7卷 250-268页

作者： Zhineng Li Ning Liu Wei Zhang Chunyu Wu Yingjie Jiang Jing Ma Mingyang Li Shunzhao Sui Key laboratory of Horticulture Science for Southern Mountains Regions Ministry of EducationChongqing Engineering Research Center for FloricultureCollege of Horticulture and Landscape ArchitectureSouthwest University400715 ChongqingChina

Chilling has a critical role in the growth and development of perennial *** chilling requirement(CR)for dormancy breaking largely depends on the ***,global warming is expected to negatively affect chilling accumulation and dormancy release in a wide range of perennial ***,we used Chimonanthus praecox as a model to investigate the CR for dormancy breaking under natural and artificial *** determined the minimum CR(570 chill units,CU)needed for chilling-induced dormancy breaking and analyzed the transcriptomes and proteomes of flowering and non-flowering flower buds(FBs,anther and ovary differentiation completed)with different *** concentrations of ABA and GA3 in the FBs were also determined using *** results indicate that chilling induced an upregulation of ABA levels and significant downregulation of SHORT VEGETATIVE PHASE(SVP)and FLOWERING LOCUS T(FT)homologs at the transcript level in FBs when the accumulated CR reached 570 CU(IB570)compared to FBs in November(***,CK)and nF16(non-flowering FBs after treatment at 16℃for−300 CU),which suggested that dormancy breaking of FBs could be regulated by the ABA-mediated SVP-FT *** in Arabidopsis was used to confirm the function of candidate genes,and early flowering was induced in 35S::CpFT1 transgenic *** data provide insight into the minimum CR(570 CU)needed for chilling-induced dormancy breaking and its underlying regulatory mechanism in ***,which provides a new tool for the artificial regulation of flowering time and a rich gene resource for controlling chilling-induced blooming.

关键词： breaking artificial analysis

Early selection efficiency for fiber dimensions and their relationships with growth and wood quality for Pinus elliotth *** southern China

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Journal of Forestry Research 2023年第6期34卷 1951-1962页

作者： Jing Wen Min Yi Leiming Dong Lu Zhang Siyu Liu Shenggui Yuan Xiaoxiao Tao Meng Lai Key laboratory of Silviculture College of ForestryJiangxi Agricultural UniversityNanchang 330045People’s Republic of China Beijing floriculture Engineering Technology Research Centre Beijing Botanical GardenBeijing 100093People’s Republic of China

A solid understanding of the efficiency of early selection for fiber dimensions is a prerequisite for breeding slash pine(Pinus elliottii Engelm.)with improved properties for pulp and paper *** correlations between size of fibers,wood quality and growth properties are also *** accomplish effective early selection for size of fibers and evaluate the impact for wood quality traits and ring widths,core samples were collected from360 trees of 20 open-pollinated Pinus elliottii families from three genetic *** were measured by SilviScan,and the age trends for phenotypic values,heritability,early-late genetic correlations,and early selection efficiency for fiber dimensions,such as tangential and radial fiber widths,fiber wall thickness and fiber coarseness,and their correlations with microfibril angle(MFA),modulus of elasticity(MOE),wood density and ring width were *** phenotypic trends were found for tangential and radial fiber widths while fiber coarseness and wall thickness curves were *** trends of heritability based on area-weighted fiber dimensions were *** to moderate heritability from pith to bark(~0.5)was found for all fiber dimension across the three sites except for tangential fiber width and wall thickness at the Ganzhou ***-late genetic correlations were 0.9 after age of 9 years,and early selection for fiber dimensions could be effective due to strong genetic *** results showed moderate to strong positive genetic correlations for modulus of elasticity and density with fiber *** effects on fiber dimensions were weak or moderate when ring width or wood quality traits were selected *** of efficiency for early selection indicated that the optimal age for radial fiber width and fiber coarseness was 6-7 years,while for tangential fiber width and wall thickness was 9-10 years.

关键词： Pinus elliottii Early selection efficiency Genetic correlation Heritability Fiber dimensions

Positive regulatory role of R2R3 MYBs in terpene biosynthesis in Lilium‘Siberia’

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Horticultural Plant Journal 2023年第5期9卷 1024-1038页

作者： Yanhong Guo Ziyu Guo Jian Zhong Yilin Liang Yuan Feng Peng Zhang Qixiang Zhang Ming Sun State Key laboratory of Efficient Production of Forest Resources Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of EducationSchool of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China

Terpenoids are the main components contributing to the fragrance of Lilium‘Siberia’,and LiTPS2 plays a critical role in the biosynthesis of *** the major terpene synthases in Lilium‘Siberia’have been identified,how these TPS genes are transcriptionally regulated remains elusive in this distinguished *** study aimed to identify transcription factors that regulate the terpene synthesis in Lilium,and disclose the related underlying transcriptional regulation *** this study,we identified three R2R3-MYB TFs—LiMYB1,LiMYB305 and LiMYB330,which were involved in regulating the biosynthesis of terpenes in Lilium‘Siberia’.Quantitative real-time PCR showed spatial and temporal expression patterns consistent with the emission patterns of terpene *** LiMYB1,LiMYB305 and LiMYB330were overexpressed in flowers,the release of some main monoterpenes,such as linalool and ocimene,as well as the expression of TPS genes,especially for LiTPS2,were enhanced.A virus-induced gene silencing(VIGS)assay showed that silencing these three LiMYBs decreased the level of monoterpenes by down-regulating the expression of the TPS *** yeast one-hybrid and transient expression assays indicated that all three LiMYBs could bind to and activate the promoter of ***,the yeast two-hybrid assay verified that LiMYB1 could interact with LiMYB308 and LiMYB330,indicating their synergistic roles in the regulation of floral terpene *** general,these results indicated that LiMYB1,LiMYB305,and LiMYB330 might play essential roles in terpene biosynthesis in Lilium and would provide a new perspective for the transcriptional regulation of volatile terpenes in flowers.

关键词： Lilium R2R3-MYB Expression analysis Terpenes Regulation

Volatile metabolome and transcriptome reveal fragrance release rhythm and molecular mechanisms of Rosa yangii

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Journal of Integrative Agriculture 2023年第7期22卷 2111-2125页

作者： ZHOU Li-jun HUANG Run-huan LIU Ting-han LIU Wei-chao CHEN Yun-yi LÜPei-feng LUO Le PAN Hui-tang YU Chao ZHANG Qi-xiang Beijing Key laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding/National Engineering Research Center for floriculture/Beijing laboratory of Urban and Rural Ecological Environment/Key laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education/School of Landscape Architecture Beijing Forestry UniversityBeijing 100083P.R.China

Rose is a highly significant ornamental plant with substantial edible and medicinal value,cultivated worldwide primarily for perfume ***,Rosa yangii,a new species found in northwestern Yunnan,China,has drawn attention due to its strong sweet scented *** this study,the floral components of *** were extracted at different flowering stages using solid phase micro extraction(SPME)and analyzed through gas chromatography-mass spectrometry(GC-MS).A total of 131 volatile organic compounds(VOCs)were detected from ***,including 69 odor *** production and release of floral VOCs were the highest during the initial-open stage,making it the most suitable time for harvesting as a significant number of floral components were synthesized and *** analysis of the odor activity values(OAV)highlighted several key aromatic ingredients of ***,such as eugenol,methyleugenol,benzeneacetaldehyde and phenylethylalcohol,heptanal,decanal,(E)-2-hexen-1-yl acetate,caryophyllene,and *** and time-order gene co-expression networks(TO-GCN)revealed that VOCs and benzenoids/phenylpropanoids,along with associated genes,played a pivotal role in the overall floral regulatory network of *** and bHLH were identified as the essential regulatory factors governing the regulation of eugenol synthase(EGS)and isoeugenol synthase(IGS),consequently influencing the sweet scent of *** findings of this study provide a scientific foundation for enhancing fragrance through molecular breeding of ornamental ***,the study facilitated the development and utilization of this new plant’s essential oil material in various industries,including food storage,aromatherapy,cosmetic,and perfumery.

关键词： rose sweet fragrance SPME-GC-MS aroma VOCs TO-GCN

SSR Analysis of Genetic Relationship and Classification in Chrysanthemum Germplasm Collection

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Horticultural Plant Journal 2018年第2期4卷 73-82页

作者： LUO Chang CHEN Dongliang CHENG Xi LIU Hua LI Yahui HUANG Conglin Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Sciences Beijing Engineering Research Center of Functional Floriculture Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology

To further test the accuracy and rationality of the Chinese classification system for chrysanthemum(Chrysanthemum morifolium), 10 simple sequence repeat(SSR) markers were used to identify a collection of 88 chrysanthemum and its related genera accessions. In total, 42 effective alleles across 88 accessions were detected; 3 429 bands were obtained by PCR amplification, including 2 630 polymorphic bands. The similarity coefficient ranged from 0.53 to 0.88. Cluster analysis based on UPGMA illustrated that the wild species and large-flower cultivars were first divided into two clusters, then the large-flower cultivars formed five distinct groups according to petal type, indicated that petal type can be a classification criterion. In the wild species cluster, C. vestitum and C. zawadaskii grouped with A. trilobata, suggested that the Ajania genera was closely related to the Chrysanthemum genera. ‘Hangbaiju’, ‘Gongju’ and ‘Chuju’ were grouped together, and ‘Boju’, O. longilobus and C. mongolicum constituted another branch, showed a correlation with geographic region of origin. Population structure analysis was subsequently performed with K values ranging from 2 to 10, and the most likely estimate for the population structure is five subpopulations, which is nearly consistent with the clustering results. Principle component analysis was further performed to verify the classification results. The results showed that these SSR markers are very powerful for studying genetic relationships and will be useful tools in the identification and classification of chrysanthemum.

关键词： chrysanthemum SSR polymorphism genetic relationship classification

The anthocyanin formation of purple leaf is associated with the activation of LfiHY5 and LfiMYB75 in crape myrtle

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Horticultural Plant Journal 2024年第5期10卷 1230-1246页

作者： Lu Feng Ping Shen Xiufeng Chi Yang Zhou Jieru Liu Tangren Cheng Jia Wang Qixiang Zhang Ming Cai Huitang Pan State Key laboratory of Efficient Production of Forest Resources Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China

Purple-leafed plants not only have a higher resistance to biotic and abiotic stresses,but also have higher ornamental *** are vital for leaf color formation,growth and development of purple ***,the molecular mechanism underlying purple leaf formation in Lagerstroemia indica remains *** and transcriptomic analysis of purple-leafed cultivar‘Ebony Embers’and greenleafed cultivar‘Arapahoe’showed that the high expression of anthocyanin structure genes induced hyperaccumulation of cyanidin and pelargonidin derivatives,making the leaves ***5,LfiMYB75 and LfibHLH1 were identified using correlation analysis and weighted gene co-expression network ***‘Arapahoe’‘Ebony Embers’population,LfiHY5 and LfiMYB75 showed significant positive correlation with leaf anthocyanin *** expression of LfiMYB75 and LfiHY5 in tobacco and purple-leafed crape myrtle indicated that the two genes activated anthocyanin *** two-hybrid analysis showed that LfiMYB75 and LfibHLH1 could form a complex that enhanced anthocyanin *** monohybrid and dual-luciferase assays confirmed that LfiHY5 activated the expression of LfiMYB75,to activate the transcription of anthocyanin structural genes LfiCHS and ***,there were three alleles of LfiHY5 in crape myrtle,and the different sequences had different activation effects on *** conclusion,the results showed that LfiHY5 led to upregulate the transcription of LfiMYB75,and LfiMYB75 formed a complex with LfibHLH1,which increased the transcription level of LfiCHS and LfiANS to affect anthocyanin synthesis in crape myrtle.

关键词： Lagerstroemia indica Leaf color Anthocyanin HY5 MYB bHLH

Identification of reference genes provides functional insights into meiotic recombination suppressors in Gerbera hybrida

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Horticultural Plant Journal 2022年第1期8卷 123-132页

作者： Fan Li Ying Cheng Lulin Ma Shenchong Li Jihua Wang floriculture Research Institute Yunnan Academy of Agricultural SciencesNational Engineering Research Center for Ornamental HorticultureKey Laboratory for Flower Breeding of Yunnan ProvinceKunming 650200China School of Agriculture Yunnan UniversityKunming 650504China

Gerbera Hybrida is one of the important cut flowers across the *** novel traits are the primarily market requirements and the breeding targets,mainly determined by the degree of genetic variation after ***,meiotic recombination is highly conserved in most eukaryotes which suppressed the crossover formation and limited the genetic ***,several meiotic recombination suppressors have been identified and characterized in plants,whereas it remains elusive in *** order to characterize the expression patterns of these suppressors in ***,20 candidate reference genes were identified from the transcriptome datasets of ***,and their expression stabilities during plant development were evaluated by geNorm,NormFinder and *** the most stable reference genes were variable in different softwares,comprehensive ranking revealed that PGK2 was the most stable reference gene and GAPDH was the most unstable *** expression patterns of FANCM,FIGL1,RECQ4,RM1,and FLIP further validated that PGK2 was suitable for normalization of gene *** study identified a reliable reference gene for gene expression during meiotic recombination,and provided functional insights into meiotic recombination suppressors in ***.

关键词： erbera hybrida Reference gene Gene expression Meiotic recombination suppressor