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Rickettsia-like organism infection associated with mass mortalities of blood clam, Tegillarca granosa, in the Yueqing Bay in China

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Acta Oceanologica Sinica 2012年第1期31卷 106-115页

作者： ZHU Zewen XU Ting HE Zhongyang WU Xinzhong WU Liuji MENG Qingguo Laboratory of Marine Life Science and Technology College of Animal SciencesZhejiang UniversityHangzhou 310029China fisheries technical extension center of zhejiang province Hangzhou 310012China

A series of mass mortalities of the cultured blood clam, Tegillarca granosa, occurred in the Yueqing Bay of China from 2005 to 2009. An obligate intracellular prokaryote, designated as rickettsia-like organism （RLO）, was frequently found in the moribund or dead blood clam sample during ultra- structural examination. These organisms were usually round, ellipsoid or occasionally dumbbell- shaped, ranged from approximately 0.28 to 0.71 #m in size and had a trilaminar cell wall. Two reproductive modes of organisms, transverse binary fission and budding, were observed. The or- ganisms were able to form eosinophilic inclusions. Most inclusions were found within epithelial and connective tissues of the mantle, gills and digestive tube. The biological and morphological char- acteristics indicate that these organisms may belong to the family Rickettsiaceae. RLOs exhibited significant pathogenicity. Cytopathological examinations revealed extensive necrosis and destruc- tion in the infected cell. The degree of tissue destruction was positively related to the number of RLO inclusions in the tissues, and the cytopathological effects were positively related to the number of intracellular RLO. RLOs and their inclusions were discovered throughout different disease areas and in different time periods. The infection intensity of the RLOs was positively correlated with the mortality rate of clams. Therefore, RLO infection might be associated with mass mortalities of cultured blood clams in the Yueqing Bay.

关键词： blood clam Tegillarca granosa rickettsia-like organism (RLO) inclusions mass mortalities

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Effects of flow velocity on water quality and ammonia excretion in recirculating aquaculture system culturing juvenile largemouth bass(Micropterus salmoides)

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International Journal of Agricultural and Biological Engineering 2022年第5期15卷 213-218页

作者： Runguo Xiao Ganxiang Wang Zhenlei Chen Zhangying Ye Songming Zhu Xueyan Ding Fan Zhou Jianlin Guo Jian Zhao College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310058China Pinghu fisheries Technology Promotion center Jiaxing 314200ZhejiangChina Ocean Academy Zhejiang UniversityZhoushan 316000ZhejiangChina zhejiang fisheries technical extension center Hangzhou 310023China Key Laboratory of Healthy Freshwater Aquaculture Ministry of Agriculture and Rural AffairsKey Laboratory of Fish Health and Nutrition of Zhejiang Province/Zhejiang Institute of Freshwater FisheriesHuzhou 313001Zhejang Chna

Flow velocity plays an important role in recirculating aquaculture systems(RAS)and the growing practice of culturing juvenile largemouth bass(Micropterus salmoides).In this study,the effects of flow velocity on the water quality as well as the ammonia excretion were discussed from the perspective of actual production,and a polynomial model of ammonia nitrogen excretion was established,using the juvenile largemouth *** showed that the range of ammonia nitrogen and nitrite nitrogen decreased with flow velocity increasing,while the number and volume share of large particles *** to the polynomial model,compared with the medium flow velocity(11 cm/s,2.45 body length(bl)/s),the ammonia excretion of juvenile largemouth bass at high(18 cm/s,4.00 bl/s),and low(4 cm/s,0.90 bl/s)flow velocity changed faster with time,and the excretion rate peaked at the 6th hour after feeding,earlier than that under medium flow ***,it is suggested to increase the flow velocity at the 5th hour after feeding and then decreased it at the 10th hour,to ensure better water quality in RAS culturing juvenile largemouth bass.

关键词： flow velocity recirculating aquaculture system juvenile largemouth bass water quality ammonia excretion

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Effects of intelligent feedingmethod on the growth,immunity and stress of juvenile Micropterus salmoides

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Artificial Intelligence in Agriculture 2021年第1期5卷 118-124页

作者： Dan Wei Fengdeng Zhang Zhangying Ye Songming Zhu Daxiong Ji Jian Zhao Fan Zhou Xueyan Ding College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310000China Ocean Academy Zhejiang UniversityZhoushan 316000China zhejiang fisheries technical extension center Hangzhou 310023China

The feeding method is of great significance for aquaculture production and *** recent years,research on intelligent feeding,a method based on fish appetite,has been ***,however,have focused on fish welfare issues that can result from intelligent *** this study,an adaptive feeder based on a practical intelligent feeding method was designed to evaluate whether this intelligent feeding method would impair fish welfare compared to traditional automatic *** results indicated that the amount of residual feed and size inhomogeneity in the traditional group was significantly higher than that in the intelligent *** results of the growth indicator showed that theweight gain rate(WGR)in the intelligent feeding groupwas significantly higher(30.17%)than in the traditional feeding *** no significant differences were observed in the survival rate(SR),the condition factor(CF)and the hepatosomatic index(HSI),the specific growth rate(SGR)in the intelligent feeding group was significantly increased by 8.33%while the feed conversion rate(FCR)was reduced by a remarkable 17.07%compared to the traditional feeding ***,intelligent feeding significantly improved the pepsin activity of the *** terms of immunity and antioxidant capacity,however,the fish in the intelligent feeding group had a significantly lower lysozyme(LZM)level than those fed with the traditional *** superoxide dismutase(SOD),the intelligent group also displayed a lower activity value,although it was not *** intelligent feeding group had 0.14 nmol/mg protein higher malondialdehyde(MDA)activity than the traditional *** stress,although no statistical significance was observed,the cortisol level in the intelligent group was 1.9 ng/ml higher than in the traditional ***,these data suggested that the intelligent feeding method can significantly improve fish growth but may also result in stress and suppress innate immunity.

关键词： Intelligent feeding method Fish welfare Growth Immunity Stress

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Identification of Species in Genus Platycephalus from Seas of China

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Journal of Ocean University of China 2020年第2期19卷 417-427页

作者： CHEN Zhi SONG Na ZOU Jianwei QIN Yan MA Lai GAO Tianxiang College of fisheries and Life Science Hainan Tropical Ocean UniversitySanya 572022China fisheries College Ocean University of ChinaQingdao 266003China Beihai fisheries technical extension Station Beihai 536000China Beihai Southern Ocean Ecological Culture Limited Company Beihai 536000China National Engineering Research center for Marine Aquaculture Zhejiang Ocean UniversityZhoushan 316022China Key Laboratory of Sustainable Utilization of Technology Research for Fishery Resource of zhejiang province Zhoushan 316021China

Platycephalus in Chinese sea area has a high commercial ***,there were mis-identifications in previous *** this study,we fully distinguished and diagnosed all five species,Platycephalus indicus,***,Platycephalus sp.,Platycephalus sp.1 and Platycephalus *** results revealed that *** was overlooked by previous *** sp.1 was misidentified as *** in reality,and Platycephalus sp.2 only existed in the seas of ***,morphological,especially phylogenetic analysis indicated that Platycephalus *** South China Sea differs from all former known species,which might be a new *** identified all Platycephalus species in China seas for the first time,which will contribute to local species identification,biodiversity conservation and sustainable exploitation of Platycephalus species.

关键词： Platycephalus seas of China new species taxonomy phylogenetics

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Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica

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Journal of Oceanology and Limnology 2018年第3期36卷 892-904页

作者： YANG Jingwen XU Yuchao XU Ke PING Hongling SHI Huilai LU Zhenming WU Changwen WANG Tianming National Engineering Research center for Facilitated Marine Aquaculture Marine Science CollegeZhejiang Ocean UniversityZhoushan 316022China Marine fisheries Research Institute of zhejiang province Zhoushan 316022China

Neuropeptide Y(NPY) has a pivotal role in the regulation of many physiological processes. In this study, the gene encoding a NPY receptor-like from the common Chinese cuttlefish Sepiella japonica( SjNPYR-like) was identified and characterized. The full-length SjNPYR-like cDNA was cloned containing a 492-bp of 5′ untranslated region(UTR), 1 182 bp open reading frame(ORF) encoding a protein of 393 amino acid residues, and 228 bp of 3′ UTR. The putative protein was predicted to have a molecular weight of 45.54 kDa and an isoelectric point(pI) of 8.13. By informatic analyses, SjNPYR-like was identified as belonging to the class A G protein coupled receptor(GPCR) family(the rhodopsin-type). The amino acid sequence contained 12 potential phosphorylation sites and five predicted N-linked glycosylation sites. Multiple sequence alignment and 3D structure modeling were conducted to clarify SjNPYR bioinformatics characteristics. Phylogenetic analysis identifies it as an NPYR with identity of 33% to Lymnaea stagnalis NPFR. Transmembrane properties of SjNPYR-like were demonstrated in vitro using HEK293 cells and the p EGFP-N1 plasmid. Relative quantifi cation of SjNPYR-like mRNA level confi rmed a high level expression and broad distribution of SjNPYR-like in various tissues of female S. japonica. In addition, the transcriptional profile of SjNPYR-like in the brain, liver, and ovary during gonadal development was analyzed. The results provide basic understanding on the molecular characteristics of SjNPYR-like and its potentially physical functions.

关键词： Sepiella japonica NPY receptor-like growth reproduction gene expression

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The Effect of Bamboo Charcoal Application on Soil Nutrients and Heavy Metals in Rice

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Phyton-International Journal of Experimental Botany 2022年第6期91卷 1245-1256页

作者： Guangyue Cai Juanli Tu Songhao Li Yifei Ruan Qifeng Wu Jiasen Wu State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou311300China Jiaxing Vocational&technical College Jiaxing314036China Agriculture and Forestry Technology extension center Hangzhou311300China

Soil heavy metal pollution is becoming more and more *** charcoal application can play an important role in alleviating the toxicity of heavy metals in *** with other biochar,bamboo charcoal has more unique properties and may have a unique effect on heavy metal *** province of Southeastern China is rich in bamboo ***,few studies related to bamboo charcoal application for heavy metal remediation in farmland were *** this study,four treatments with different amounts of bamboo charcoal application were set up through a field experiment,namely BC0(no bamboo charcoal application),BC1(2500 kg⋅ha^(−1)),BC2(5000 kg⋅ha^(−1)),and BC3(10000 kg⋅ha^(−1)),and each treatment was replicated three *** results showed that(1)The application of bamboo charcoal significantly increased the soil pH and organic matter *** with BC0,the pH and organic content of BC3 increased by 7.4%and 17.4%(P0.05).(3)With the increase of bamboo charcoal application,the Cd content in rice gradually decreased,the BC3 treatment significantly decreased by 39.0%(P0.05),compared with BC0.(4)Soil pH,organic matter and Cd in rice seeds were significantly negatively correlated(P<0.01).The heavy metal content in rice does not change with the change of heavy metal content with HCl-extractable state in *** means bamboo charcoal does not reduce heavy metal content in rice by simply declining the heavy metal content with HCl-extractable *** mechanism of action is relatively complicated,and further study is needed.

关键词： Bamboo charcoal rice heavy metal pollution paddy field HCl-extractable heavy metal

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Dual RNA-seq provides novel insight into the roles of dks A from Pseudomonas plecoglossicida in pathogenhost interactions with large yellow croakers(Larimichthys crocea)

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Zoological Research 2020年第4期41卷 410-422页

作者： Lu-Ying Wang Zi-XuLiu Ling-Min Zhao Li-Xing Huang Ying-Xue Qin Yong-Quan Su Wei-Qiang Zheng Fan Wang Qing-Pi Yan fisheries College Jimei UniversityXiamenFujian 361021China State Key Laboratory of Large Yellow Croaker Breeding Ningde Fufa Aquatic Products Co.Ltd.NingdeFujian 352000China Fujian Provincial Fishery technical extension center FuzhouFujian 350003China

Pseudomonas plecoglossicida is a rod-shaped,gram-negative bacterium with *** causes visceral white spot disease and high mortality in Larimichthys crocea during culture,resulting in serious economic *** of transcriptome and quantitative real-time polymerase chain reaction(PCR)data showed that dks A gene expression was significantly up-regulated after 48 h of infection with Epinephelus coioides(log2 FC=3.12,P<0.001).RNAi of five sh RNAs significantly reduced the expression of dks A in ***,and the optimal silencing efficiency was 96.23%.Compared with wild-type strains,the symptoms of visceral white spot disease in *** infected with RNAi strains were reduced,with time of death delayed by 48 h and mortality reduced by 25%.The dks A silencing led to a substantial down-regulation in cellular component-,flagellum-,and ribosome assembly-related genes in ***,and the significant up-regulation of fli C may be a way in which virulence is maintained in *** GO and KEGG results showed that RNAi strain infection in *** led to the down-regulation of inflammatory factor genes in immune-related pathways,which were associated with multiple immune response *** also showed that dks A was a virulence gene in *** with the wild-type strains,RNAi strain infection induced a weaker immune response in ***.

关键词： mortality infection yellow

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Eye location, cranial asymmetry, and swimming behavior of different variants of Solea senegalensis

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Aquaculture and fisheries 2020年第4期5卷 182-186页

作者： Jubin Xing Zhonghe Ke Liping Liu Chenhong Li Xiaoling Gong Baolong Bao Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University) Ministry of EducationInternational Research Center for Marine Biosciences at Shanghai Ocean UniversityMinistry of Science and TechnologyNational Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghai201306China Fishery technical extension Station of Zhuji City ZhujiZhejiang311800China

Many flatfish species,such as Scophthalmus maximus,Paralichthys olivaceus,Solea senegalensis,are extensively cultured *** flatfishes,eye migration varies among different species and even within the same *** genetic mechanism underlying eye migration is still unclear,and it is the key to improve metamorphosis in flatfish aquaculture and understand flatfish *** this study,the high frequency of eye location variants in an artificial *** population allowed us to investigate the eye preference during migration;completeness of the eye migration;and other related left-right asymmetric characteristics,such as asymmetrical skull remodeling and lying down on one *** different frequencies of eye preference during migration,completeness of the eye migration,and settling side suggest that they are determined by different genotypes and imply independent evolutionary events during flatfish *** found that the swimming time may be related to the completeness of eye migration,and further the migration of the eye,the more time the fish spent at the sea *** left-right asymmetric degrees of not only the frontal bones and lateral ethmoids but also the jaw bone,including the premaxillary,maxillary,and dentary bones,depend on the completeness of eye mi***,we proposed a hypothesis for the asymmetric origin of flatfish that provides reasonable explanations for the selective advantages of primitive flatfish species.

关键词： Flatfish Eye migration Left/right asymmetry Senegalese sole

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Main Methods Applied in Fertigation Technology

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Asian Agricultural Research 2016年第7期8卷 56-60页

作者： Xijing CHEN Linbing ZHONG Shugen GUO Qi ZHANG Hui XI Institute of Environment ResourceSoil and FertilizerZhejiang Academy of Agricultural Sciences Tonglu County Agricultural Technology extension center of zhejiang province Lanxi City Agricultural Technology extension center of zhejiang province

In order to better promote extension and application of fertigation technology,this paper elaborates major technical issues. It presents pipeline diameter control and increasing the uniformity in selection of methods of irrigation system types and in the process of system design. Besides,it introduces characteristics and use methods of main fertilizer application equipment,filter,and control devices. In addition,it lists amount of fertilizer application and irrigation according to target yield of crops,and describes major attentions for selection of fertilizers,irrigation,and soil moisture control.

关键词： Fertigation Irrigation Fertilizer application Drip irrigation Micro jet irrigation

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Effect of different pruning operations on the plant growth,phytohormones and transcriptome profiles of the following spring tea shoots

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Beverage Plant Research 2022年第1期2卷 85-93页

作者： Lu Lu Wenwen Luo Yanan Zheng Jing Jin Ruyi Liu Yiqing Lv Ying Ye Jianhui Ye Tea Research Institute Zhejiang UniversityHangzhou 310013China Jinhua Department of Economic Specialty Technology Promotion 828 South Shuanglong RoadJinhua 321000China zhejiang Agricultural technical extension center 29 Fengqidong RoadHangzhou 310020China

Pruning as an important agronomic operation plays a crucial role in the regulation of tea plant growth,development and dry tea *** effects of different pruning operations on the plant growth,phytohormones and transcriptome profiles of the following spring tea shoots were ***-treated samples had generally increased median of the weight of 100 buds(two leaves and one bud,15.5−20.5 g)and longer stem diameter(1.7−1.8 mm)whereas shorter shoot length(34.6−59.2 mm)at the stage of two leaves and one bud,compared with unpruned samples(14.0 g,1.7 mm,87.4 mm),among which heavy pruning in mid April and early May greatly accelerated the development of the following spring tea *** levels of phytohormones(auxin,gibberellin 1,gibberellin 3,and trans-zeatin)were significantly increased in the spring buds of tea plants heavily pruned in *** KEGG result indicated that the pathways of plant–pathogen interaction,plant hormone signal transduction and circadian rhythm were regulated by different pruning *** pruning in April or May,without autumn pruning was suitable for producing premium green tea due to the early development and the higher weight of 100 *** study provides scientific guidance to regulate the growth of the following spring tea shoots using plant pruning operations.

关键词： spring operations pruning

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