检索结果-南通市图书馆

Knockdown of RCN1 contributes to the apoptosis of colorectal cancer via regulating IP3R1

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2024年第5期48卷 835-845页

作者： XUAN SHI YUFEN WANG CHENYU LI WANGSHU FU XINYUE ZHANG AIXIA GONG Department of Gastroenterology The First Affiliated Hospital of Dalian Medical UniversityDalianChina Department of Gastroenterology The First Affiliated Hospital of Jinzhou Medical UniversityJinzhouChina The first Clinical College Dalian Medical UniversityDalianChina

Background:The incidence of colorectal cancer(CRC)has been increasing in recent ***,the discovery of factors that can assist in alleviating CRC is urgently ***:To identify a potential factor involved in the development of CRC,we screened the upregulated genes in tumor tissues through four datasets from an online *** expression of reticulocalbin 1(RCN1),a Ca2+-binding protein,was upregulated in the four *** on loss-offunction experiments,the effect of RCN1 on cell viability was assessed by Cell Counting Kit-8(CCK-8)*** regulatory effect of RCN1 on apoptosis was evaluated through Annexin V-fluorescein 5-isothiocyanate(FITC)/propidium iodide(PI)staining assay and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL)assay in RKO and SW480 *** of endoplasmic reticulum(ER)stress signaling pathways was confirmed by estimating the phosphorylation and expression of PRKR-like ER kinase(PERK),inositol-requiring kinase-1(IRE1),transcription factor 6(ACT6),and CCAAT/enhancer-binding protein-homologous protein(CHOP).The intracellular Ca2+homeostasis regulated by RCN1 was determined through the detection of Ca2+concentration and mitochondrial membrane potential(MMP)***,whether inositol 1,4,5-trisphosphate receptor type 1(IP3R1)was involved in the regulation of RCN1 in CRC was verified through the depletion of IP3R1 in RKO ***:Knockdown of RCN1 reduced cell viability and facilitated apoptosis in RKO and SW480 *** of PERK and IRE1,activation of ATF6,and upregulation of CHOP were induced by the absence of RCN1,suggesting that the unfolded protein response(UPR)was activated in CRC *** concentration of Ca2+in mitochondria was increased after RCN1 depletion,followed by reduction in the MMP and release of cytochrome c from mitochondria to the cytoplasm in RKO and SW480 ***,it was demonstrated that IP3R1 mediates the effect of RCN1 on apoptosis i

关键词： Reticulocalbin 1 Unfolded protein response IP3R1 Colorectal cancer

Effect of screening colonoscopy frequency on colorectal cancer mortality in patients with a family history of colorectal cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2024年第2期16卷 354-363页

作者： Li Zheng Bin Li Ling Lei Li-Jia Wang Zhi-Ping Zeng Jian-Dong Yang Department of Gastroenterology The First Hospital of NanchangNanchang 330008Jiangxi ProvinceChina Department of General Surgery The First Hospital of NanchangNanchang 330008Jiangxi ProvinceChina

BACKGROUND Colorectal cancer is a common malignant tumor in China,and its incidence in the elderly is increasing *** bowel disease is a group of chronic non-specific intestinal inflammatory diseases,including ulcerative colitis and Crohn’s *** included the clinicopathological and follow-up data of patients with colorectal cancer who underwent laparoscopic colectomy or open colectomy at our Gastrointestinal Department between January 2019 and December *** indicators,oncological indicators,and survival rates were compared between the *** results of 104 patients who met the above criteria were extracted from the database(laparoscopic colectomy group=63,open colectomy group=41),and there were no statistically significant differences in the baseline data or follow-up time between the two *** Intraoperative blood loss,time to first ambulation,and time to first fluid intake were significantly lower in the laparoscopic colectomy group than in the open colectomy *** differences in overall mortality,tumor-related mortality,and recurrence rates between the two groups were not statistically significant,and survival analysis showed that the differences in the cumulative overall survival,tumor-related survival,and cumulative recurrence-free rates between the two groups were not statistically *** In elderly patients with colorectal cancer,laparoscopic colectomy has better short-term outcomes than open colectomy,and laparoscopic colectomy has superior long-term survival outcomes compared with open colectomy.

关键词： Colorectal cancer Laparoscopic surgery Open surgery Prognosis Laparoscopic colectomy Open colectomy

MGMT activated by Wnt pathway promotes cisplatin tolerance through inducing slow-cycling cells and nonhomologous end joining in colorectal cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2024年第6期14卷 863-877页

作者： Haowei Zhang Qixin Li Xiaolong Guo Hong Wu Chenhao Hu Gaixia Liu Tianyu Yu Xiake Hu Quanpeng Qiu Gang Guo Junjun She Yinnan Chen Department of General Surgery The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an710061China Center for Gut Microbiome Research Med-X InstituteThe First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an710061China Department of High Talent The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an710061China

Chemotherapy resistance plays a pivotal role in the prognosis and therapeutic failure of patients with colorectal cancer(CRC).Cisplatin(DDP)-resistant cells exhibit an inherent ability to evade the toxic chemotherapeutic drug effects which are characterized by the activation of slow-cycle programs and DNA *** the elements that lead to DDP resistance,O^(6)-methylguanine(O^(6)-MG)-DNA-methyltransferase(MGMT),a DNA-repair enzyme,performs a quintessential *** this study,we clarify the significant involvement of MGMT in conferring DDP resistance in CRC,elucidating the underlying mechanism of the regulatory actions of MGMT.A notable upregulation of MGMT in DDP-resistant cancer cells was found in our study,and MGMT repression amplifies the sensitivity of these cells to DDP treatment in vitro and in ***,in cancer cells,MGMT overexpression abolishes their sensitivity to DDP ***,the interaction between MGMT and cyclin dependent kinase 1(CDK1)inducing slow-cycling cells is attainted via the promotion of ubiquitination degradation of ***,to achieve nonhomologous end joining,MGMT interacts with XRCC6 to resist chemotherapy *** transcriptome data from samples of 88 patients with CRC suggest that MGMT expression is co-related with the Wnt signaling pathway activation,and several Wnt inhibitors can repress drug-resistant *** summary,our results point out that MGMT is a potential therapeutic target and predictive marker of chemoresistance in CRC.

关键词： Colorectal cancer MGMT Chemotherapy resistance Slow-cycling cells Nonhomologous end joining Wnt pathway

LncRNA LOC103694972 promotes fibrosis of NRK-49F cells by regulating STAT3-dependent Smad/CTGF pathway via targeting miR-29c-3p

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2024年第3期48卷 501-511页

作者： YAN LI HUZHI CAI XIAOLING PENG YOUHUI LIU QINGYANG CHEN XIANGDONG LIN XINYU CHEN Department of Vascular and Tumor Intervention The First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of Intensive Care Unit The First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of Nursing Research The First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of Intensivie Care Unit The First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of Endocrinology The First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of Preventive Treatment Center The First Hospital of Hunan University of Chinese MedicineChangsha410007China

Background:Renalﬁbrosis is an important process in the development of chronic kidney *** the pathogenesis andﬁnding effective treatments for renalﬁbrosis is *** study aims to investigate whether a newly discovered long non-coding RNA(lncRNA)called LOC103694972 could be a potential target for treatingﬁbrosis of NRK-49F ***:LncRNA Chip was used to identify differentially expressed lncRNAs between TGF-β1-induced NRK-49F cells and normal *** dual-luciferase assay conﬁrmed the binding between miR-29c-3p and signal transducer and activator of transcription(STAT3),as well as between miR-29c-3p and lncRNA ***-LOC103694972 and miR-29c-3p mimic were then transfected into TGF-β1-induced NRK-49F ***:The study found that LOC103694972 was highly expressed in TGF-β1-induced NRK-49F *** cells exhibited increased cell length and activity compared to the control *** expression levels of Collagen I,α-Smooth muscle actin(α-SMA),and tissue inhibitor of metalloproteinase(TIMP-1)were increased,while matrix Metalloproteinase 2(MMP2)and matrix Metalloproteinase 9(MMP9)expression was ***,transfection with si-LOC103694972 and miR-29c-3p mimics restored cell morphology and reduced cell *** led to a decrease in the levels of Collagen I,α-SMA,and TIMP-1,as well as an increase in MMP2 and MMP9 ***,TGF-β1-induced NRK-49F cells transfected with miR-29c-3p mimics activated the STAT3-Smad3/CTGF ***:Based on theseﬁndings,lncRNA LOC103694972 shows promise as a target for treating renalﬁ*** negatively regulates miR-29c-3p and activates the STAT3-Smad3/CTGF pathway.

关键词： Renal fibrosis lncRNA LOC103694972 lncRNA Chip miR-29c-3p STAT3-Smad3/CTGF

Profibrotic role of the SOX9-MMP10-ECM biosynthesis axis in the tracheal fibrosis after injury and repair

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第5期11卷 360-373页

作者： Lei Gu Anmao Li Chunyan He Rui Xiao Jiaxin Liao Li Xu Junhao Mu Xiaohui Wang Mingjin Yang Jinyue Jiang Yang Bai Xingxing Jin Meiling Xiao Xia Zhang Tairong Tan Yang Xiao Jing Lin Yishi Li Shuliang Guo Department of Respiratory and Critical Care Medicine The First Affiliated Hospital of Soochow UniversitySuzhou 215006China Department of Respiratory and Critical Care Medicine The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Infection Disease The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang 325000China

Fibroblast activation and extracellular matrix(ECM)deposition play an important role in the tracheal abnormal repair process and *** a transcription factor,SOX9 is involved in fibroblast activation and ECM ***,the mechanism of how SOX9 regulates fibrosis after tracheal injury remains *** investigated the role of SOX9 in TGF-b1-induced fibroblast activation and ECM deposition in rat tracheal fibroblast(RTF)***9 overexpression adenovirus(Ad-SOX9)and siRNA were transfected into RTF *** found that SOX9 expression was up-regulated in RTF cells treated with ***9 overexpression activated fibroblasts and promoted ECM *** SOX9 inhibited cell proliferation,migration,and ECM deposition,induced G2 arrest,and increased apoptosis in RTF ***-seq and chromatin immunoprecipitation sequencing(ChIP-seq)assays identified MMP10,a matrix metalloproteinase involved in ECM deposition,as a direct target of SOX9,which promotes ECM degradation by increasing MMP10 expression through the Wnt/b-catenin signaling ***,in vivo,SOX9 knockdown ameliorated granulation proliferation and tracheal fibrosis,as manifested by reduced tracheal *** conclusion,our findings indicate that SOX9 can drive fibroblast activation,cell proliferation,and apoptosis resistance in tracheal fibrosis via the Wnt/b-catenin signaling *** SOX9eMMP10 eECM biosynthesis axis plays an important role in tracheal injury and *** SOX9 and its downstream target MMP10 may represent a promising therapeutic approach for tracheal fibrosis.

关键词： Extracellular matrix deposition Fibroblast activation MMP10 SOX9 Tracheal fibrosis

A prognosis model for predicting immunotherapy response of esophageal cancer based on oxidative stress-related signatures

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oncology Research 2024年第1期32卷 199-212页

作者： JING GUO CHANGYONG TONG JIANGUANG SHI XINJIAN LI XUEQIN CHEN Department of Thoracic Surgery The First Affiliated Hospital of Ningbo UniversityNingboChina Department of Chinese Traditional Medicine The First Affiliated Hospital of Ningbo UniversityNingboChina

Oxidative stress(OS)is intimately associated with tumorigenesis and has been considered a potential therapeutic ***,the OS-associated therapeutic target for esophageal squamous cell carcinoma(ESCC)remains *** our study,gene expression data of ESCC and clinical information from public databases were *** LASSO-Cox regression analysis,a risk score(RS)signature map of prognosis was constructed and performed external verification with the GSE53625 *** ESTIMATE,xCell,CIBERSORT,TIMER,and ImmuCellAI algorithms were employed to analyze infiltrating immune cells and generate an immune microenvironment(IM).Afterward,functional enrichment analysis clarified the underlying mechanism of the *** was utilized for forecasting the survival rate of individual ESCC *** a result,we successfully constructed an OS-related genes(OSRGs)model and found that the survival rate of high-risk groups was lower than that of low-risk *** AUC of the ROC verified the strong prediction performance of the signal in these two cohorts *** to independent prognostic analysis,the RS was identified as an independent risk factor for *** nomogram and follow-up data revealed that the RS possesses favorable predictive value for the prognosis of ESCC ***-PCR detection demonstrated increased expression of MPC1,COX6C,CYB5R3,CASP7,and CYCS in esophageal cancer *** conclusion,we have constructed an OSRGs model for ESCC to predict patients’prognosis,offering a novel insight into the potential application of the OSRGs model in ESCC.

关键词： Esophageal carcinoma OS Prognosis Risk markers Immune cell infiltration

Aggressive fluid management may be associated with disease progression in suspected sepsis patients admitted to the intensive care unit: a retrospective cohort study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Emergency Medicine 2024年第1期15卷 52-55页

作者： Miao Bian Zhihao Wang Yanling Chen Yue Sun Hongsen Ji Yutao Wang Li Pang Department of Respiratory Qianwei Hospital of Jilin ProvinceChangchun 130012China Department of Geriatrics Jilin Provincial Geriatric Medicine Clinical Research Centerthe First Hospital of Jilin UniversityChangchun 130021China Department of Emergency the First Hospital of Jilin UniversityChangchun 130021China The first Operating Room the First Hospital of Jilin UniversityChangchun 130021China

Sepsis is a lethal condition characterized by multiple organ dysfunction due to disrupted host responses to severe infections.[1]Aff ected patients often have a Sequential Organ Failure Assessment(SOFA)score≥2.[2]Patients with a SOFA score<2 and at least one of the following were considered as“suspected sepsis”:(1)quick SOFA(qSOFA)score≥2;(2)SOFA score=1;or(3)National Early Warning Score(NEWS)4-6.[3]Compared with studies on fluid resuscitation in sepsis patients,there are few studies on fluid management in patients with suspected ***,we conducted a retrospective cohort study to evaluate the relationship between fluid management and disease progression in suspected sepsis patients.

关键词： patients sepsis admitted

Real-Time 3D Ultrasound Imaging System Based on a Hybrid Reconstruction Algorithm

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Electronics 2024年第1期33卷 245-255页

作者： Yifei LYU Yu SHEN Mingbo ZHANG Junchen WANG School of Mechanical Engineering and Automation Beihang University The first Medical Center General Hospital of Chinese PLA

As a safe and convenient imaging technology in clinical routine diagnosis, ultrasound imaging can provide real-time 2D images of internal tissues and organs. To realize real-time 3D image reconstruction, pixel nearest neighbor interpolation(PNN) reconstruction algorithm and Bezier interpolation algorithm are combined into a hybrid reconstruction algorithm. On this basis, a real-time interactive 3D ultrasound imaging system is *** temporal calibration and spatial calibration, the six degrees of freedom poses of 2D ultrasound images can be accurately collected. The 3D volume reconstructed by the proposed 3D reconstruction algorithm is visualized by volume rendering. A multi-thread software system allows parallel operation of data acquisition, 3D reconstruction,volume visualization and other functions. 3D imaging experiments on a 3D printing femur model, a neck phantom and the neck of human volunteers were performed for systematic evaluation. When the reconstruction voxel size was set to be(0.53 mm3, 1.03 mm3, 1.53 mm3), the reconstruction errors of the femur and trachea model were respectively(0.23 mm, 0.31 mm, 0.56 mm) and(0.62 mm, 0.88 mm, 1.41 mm). Clinical feasibility was demonstrated by application of the 3D ultrasound imaging on the neck of human volunteers.

关键词： Interpolation Three-dimensional displays Ultrasonic imaging Imaging Data visualization Reconstruction algorithms Real-time systems

Identification prognostic features related to sphingolipid metabolism and experimental validation of TRIM47 in hepatocellular carcinoma

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2024年第4期48卷 639-651页

作者： JIAN TANG CHENQIANG ZHU YUN CHEN YUNLONG WU MING HE YI ZHOU MINGHUA XIE Department of Intensive Care Unit The First People’s Hospital of Linping DistrictHangzhouChina Department of Pharmacy The First People’s Hospital of Linping DistrictHangzhouChina

Background:The speciﬁc impact of sphingolipid metabolism on developing hepatocellular Carcinoma(HCC)remains *** study aims to explore the relationship between sphingolipid metabolism and HCC prognosis,immune response,and drug ***:Data were obtained from The Cancer Genome Atlas(TCGA)-Hepatocellular Carcinoma(LIHC)and Gene Expression Omnibus(GEO,GSE14520 datasets).47 sphingolipid metabolism genes were obtained from the Kyoto Encyclopedia of Genes and Genomes(KEGG)*** classifying HCC samples using the Non-negative Matrix Factorization(NMF)clustering method,differentially expressed genes were ***,8 risk genes were obtained by univariate analysis,survival random forest reduction and lasso *** expression of 8 risk genes was veriﬁed in ***:8 risk genes were used to construct the Sphingolipid score ***-Sphingolipid score predicted poor prognosis of HCC *** score was associated with immune checkpoints(IL-1B,TLR4,TGFB1,and IL-10),immune cells(Th2,Treg,MDSC,Neutrophil,Fibroblasts and macrophage),and MAPK *** the High-Sphingolipid score group,a signiﬁcantly higher proportion of patients with TP53(p53)mutations was signiﬁcantly higher(56%).Furthermore,patients with a high-Sphingolipid score were predicted to have a higher sensitivity to chemotherapy *** vitro validation showed that compared with normal liver cells LX-2,TRIM47,and S100A9 signiﬁcantly increased in liver cancer cells Hep G2,MHCC-97H,and Hep3B2.1-7,while SLC1A7,LPCAT1,and CFHR4 signiﬁcantly *** TRIM47 reduced the proliferation and promoted *** levels of ceramide synthesis-related indexes(CERS1,CERS6,CERS5,and SPTLC2)increased,and the ACER3 related to catalytic hydrolysis ***:We constructed a sphingolipid metabolism-related prognostic signature(Sphingolipid score)based on 8 risk ***47 may affect the development of liver cancer by regulating the relevant indicators of

关键词： Hepatocellular carcinoma Sphingolipid metabolism TRIM47 Prognosis