LncRNA LOC103694972 promotes fibrosis of NRK-49F cells by regulating STAT3-dependent Smad/CTGF pathway via targeting miR-29c-3p

作     者：YAN LI HUZHI CAI XIAOLING PENG YOUHUI LIU QINGYANG CHEN XIANGDONG LIN XINYU CHEN 

作者机构：Department of Vascular and Tumor InterventionThe First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of Intensive Care UnitThe First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of Nursing ResearchThe First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of Intensivie Care UnitThe First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of EndocrinologyThe First Hospital of Hunan University of Chinese MedicineChangsha410007China Department of Preventive Treatment CenterThe First Hospital of Hunan University of Chinese MedicineChangsha410007China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2024年第48卷第3期

页      面：501-511页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：This work was supported by the Hunan Provincial Education Department General Project Research Fund(No.20C1412) the Hunan Graduate Scientific Research Innovation Project(No.CX2018B474) the National Famous Elderly Chinese Medicine Experts Xinyu Chen Inheritance Workshop Construction Project(No.75) 

主　　题：Renal fibrosis lncRNA LOC103694972 lncRNA Chip miR-29c-3p STAT3-Smad3/CTGF 

摘      要：Background:Renalfibrosis is an important process in the development of chronic kidney *** the pathogenesis andfinding effective treatments for renalfibrosis is *** study aims to investigate whether a newly discovered long non-coding RNA(lncRNA)called LOC103694972 could be a potential target for treatingfibrosis of NRK-49F ***:LncRNA Chip was used to identify differentially expressed lncRNAs between TGF-β1-induced NRK-49F cells and normal *** dual-luciferase assay confirmed the binding between miR-29c-3p and signal transducer and activator of transcription(STAT3),as well as between miR-29c-3p and lncRNA ***-LOC103694972 and miR-29c-3p mimic were then transfected into TGF-β1-induced NRK-49F ***:The study found that LOC103694972 was highly expressed in TGF-β1-induced NRK-49F *** cells exhibited increased cell length and activity compared to the control *** expression levels of Collagen I,α-Smooth muscle actin(α-SMA),and tissue inhibitor of metalloproteinase(TIMP-1)were increased,while matrix Metalloproteinase 2(MMP2)and matrix Metalloproteinase 9(MMP9)expression was ***,transfection with si-LOC103694972 and miR-29c-3p mimics restored cell morphology and reduced cell *** led to a decrease in the levels of Collagen I,α-SMA,and TIMP-1,as well as an increase in MMP2 and MMP9 ***,TGF-β1-induced NRK-49F cells transfected with miR-29c-3p mimics activated the STAT3-Smad3/CTGF ***:Based on thesefindings,lncRNA LOC103694972 shows promise as a target for treating renalfi*** negatively regulates miR-29c-3p and activates the STAT3-Smad3/CTGF pathway.