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Health 2012年第9期4卷 660-666页

作者： Javier García-Ferreyra Pedro Valdivieso Milton Zambrano Jorge Carpio fertilab laboratory of assisted reproduction Lima Perú laboratory of assisted reproduction Alcival Hospital Guayaquil Ecuador

Purpose: Evaluate the effect of preincubation of oocytes prior to IVF or ICSI cycles with embryo transfer at blastocyst stage. Methods: Retrospective non randomized study based on secondary analysis of data. Setting: laboratory of assisted reproduction at the Alcivar Hospital. Patients: One hundred-eighteen cycles of IVF and ICSI were analyzed in the present study. The evaluated groups were formed for those patients whose oocytes, after retrieval, were inseminated at 1-3 h (Group I) or 4-6 h (Group II). Results: There was no difference in fertilization rate (83.6% and 78.1%), Day 3 cleavage rate (95.1% and 97.1%), and blastocyst formation (31.1% and 39.1%) for groups I and II respectively. Clinical pregnancy rates (PR: 53.0% vs 22.9%) and implantation rates (IR: 38.1% vs 13.0%) were significantly higher in group II versus group I, respectively (P < 0.05). Conclusions: Preincubation of oocytes before insemination is a factor which raises the PR and IR after the blastocyst transfer.

关键词： Oocytes Embryo Development Maturation Blastocyst ART

Advances in preimplantation genetic diagnosis/screening

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Science China(Life Sciences) 2014年第7期57卷 665-671页

作者： YAN LiYing WEI Yuan HUANG Jin ZHU XiaoHui SHI XiaoDan XIA Xi YAN Jie LU CuiLing LIAN Ying LI Rong LIU Ping QIAO Jie Department of Obstetrics and Gynecology Peking University Third Hospital Key laboratory of assisted reproduction ministry of education Beijing Key laboratory of Reproductive Endocrinology and assisted reproduction

Preimplantation genetic diagnosis(PGD)gives couples who have a high risk of transmitting genetic disorders to their baby the chance to have a healthy offspring through embryo genetic analysis and *** genetic screening(PGS)is an effective method to select euploid embryos that may prevent repeated implantation failure or ***,how and to whom PGS should be provided is a controversial *** first successful case of PGD of a human being was reported in 1990,and there have been tremendous improvements in this technology since *** embryo biopsy and genetic technologies have been improved dramatically,which increase the accuracy and expand the indications of PGD/PGS.

关键词： preimplantation genetic diagnosis preimplantation genetic screening indications biopsy methods array comparative genomic hybridization next-generation sequencing

Sperm origins and concentration do not impact the clinical outcomes in intracytoplasmic sperm injection cycles

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Asian Journal of Andrology 2018年第5期20卷 454-458页

作者： Cen Yang Ze-Hong Zhou Dan-Ni Zheng Xiao-Fei XU Jin Huang Ying Lian Jie Qiao Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital Beijing 100191 China Beijing Key laboratory of Reproductive Endocrinology and assisted reproduction Technology Beijing 100191 China Key laboratory of assisted reproduction Ministry Of Education Beijing 100191 China

In the present study, we evaluated the impact of sperm origins and concentration on the clinical outcomes of intracytoplasmic sperm injection （ICSI） cycles. A total of 1201 IC51 cycles were retrospectively analyzed for male azoospermia or oligozoospermia between January 2015 and December 2015 in the Peking University Third Hospital. Patients were divided into three groups （Group 1 vs Group 2/3; surgically extracted sperm vs ejaculated sperms）： Group 1 included 343 ICSI cycles and Group 2 analyzed 388 cycles on semen with sperm concentration 〈5 × 10^6 m1-1 （severe oligozoospermia group）. Group 3 included 470 cycles with sperm concentration between 5×10^6 m1-1 and 15 × 10^6 m1-1 （mild oligozoospermia group）. Fertilization rates, clinical pregnancy rates, and live birth rates were analyzed and compared among groups of different semen origins and concentrations on the oocyte retrieval day. Group 2 showed a lower fertilization rate than Group 3 （62.9%± 21.6% vs 66.8% ± 22.1%, P 〈 0.05）. There were no statistically significant differences in clinical pregnancy rate per transfer （51.3%, 46.7%, and 50.0%, respectively）, live birth rate per transfer （44.4%, 40.9%, and 41.4%, respectively）, accumulative live birth rate （58.3%, 51.0%, and 52.1%, respectively）, twin birth rate （18.4%, 10.6%, and 12.6%, respectively）, and birth defects rate （0, 0.3%, and 0.2%, respectively） among three groups. The results of this study indicated that sperm origins and concentration do not imDact the clinical outcomes in ICSI cycles.

关键词： azoospermia clinical outcomes intracytoplasmic sperm injection oligozoospermia sperm concentration

Dual role for the unfolded protein response in the ovary： adaption and apoptosis

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Protein & Cell 2017年第1期8卷 14-24页

作者： Ning Huang Yang Yu Jie Qiao Beijing Key laboratory of Reproductive Endocrinology and assisted Reproductive Technology and Key laboratory of assisted reproduction Ministry of Education Center of Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital Beijing 100191 China

The endoplasmic reticulum （ER） is the principal organelle responsible for several specific cellular functions including synthesis and folding of secretory or membrane proteins, lipid metabolism, and Ca^2＋ storage. Different physiological as well as pathological stress conditions can, however, perturb ER homeostasis, giving rise to an accumulation of unfolded or misfolded proteins in the ER lumen, a condition termed ER stress. To deal with an increased folding demand, cells activate the unfolded protein response （UPR）, which is initially protective but can become detrimental if ER stress is severe and prolonged. Accumulating evidence demonstrates a link between the UPR and ovarian development and function, including follicular growth and maturation, follicular atresia, and corpus luteum biogenesis. Additionally, ER stress and the UPR may also play an important role in the ovary under pathological conditions. Understanding the molecular mechanisms related to the dual role of unfolded protein response in the ovarian physiology and pathology may reveal the pathogenesis of some reproductive endocrine diseases and provide a new guidance to improve the assisted reproductive technology. Here we review the current literature and discuss concepts and progress in understanding the UPR, and we also analyze the role of ER stress and the UPR in the ovary.

关键词： endoplasmic reticulum stress obesity,ovary follicle ovarian diseases

Multiple factors affecting surgical outcomes and patency rates in use of single-armed two-suture microsurgical vasoepididymostomy： a single surgeon＇s experience with 81 patients

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Asian Journal of Andrology 2016年第1期18卷 129-133页

作者： Kai Hong Lian-Ming Zhao Shi-Xing Xu Wen-Hao Tang Jia-Ming Mao De-Feng Liu Hui Jiang Lu-Lin Ma Jie Qiao Department of Urology Peking University Third Hospital Beijing China Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital Beijing China Key laboratory of assisted reproduction Ministry of Education Beijing China Beijing Key laboratory of Reproductive Endocrinology and assisted reproduction Beijing China

Vasoepididymostomy （VE）, as the most challenging procedure in microsurgeries, is often carried out with a double-armed two-suture technique. In this study, we evaluated the efficacy and safety of the single-armed two-suture VEs on humans and studied the factors that could possibly affect the patency rates. From July 2012 to July 2013, we reviewed 81 patients with consecutive primary epididymal obstruction who underwent single-armed two-suture longitudinal intussusception microsurgical VEs by a single surgeon, Kai Hong （KH）. At the same time, we analyzed seven factors that possibly related to the patency rates. With the single-armed technique, a total of 81 men underwent the microsurgical VEs. Data on 62 patients were completely recorded. 19 patients were lost to follow-up. Mean age was 31 years old. Mean follow-up time was 8.8 （2-17） months. The patency rate was 66.1% （41/62）. Natural pregnancy rate was 34.1% （14/41）. Overall pregnancy rate was 22.6% （14/62）. No severe surgical complications were noted. With logistic regression test analysis, there were two factors related to a higher patency rate： anastomosis sites （P = 0.035） and motile sperm found in the epididymal fluid （P = 0.006）. Motile sperm found in the epididymal fluid were associated with a higher patency rate （OR = 11.80, 95% CI = 1.79, 77.65）. The single-armed two-suture longitudinal VE technique is feasible for microsurgical practice. The patency and pregnancy rates are comparable to the doubled-armed technique. Anastomosis sites and motile sperm found in the epididymal fluid were the most two important factors related to higher patency.

关键词： male infertility microsurgery obstructive azoospermia vasoepididymostomy

Effects of malignancies on fertility preservation outcomes and relevant cryobiological advances

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Science China(Life Sciences) 2020年第2期63卷 217-227页

作者： Dongming Liu Jie Yan Jie Qiao Reproductive Medical Center Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China National Clinical Center for Obstetrics and Gynecology Beijing 100191China Key laboratory of assisted reproduction Ministry of EducationBeijing 100191China Beijing Key laboratory of Reproductive Endocrinology and assisted reproduction Beijing 100191China

A decrease in cancer deaths has resulted in the possibility of child bearing for many young adult cancer survivors. Most antitumor treatment modalities are detrimental to female fertility, and methods for fertility preservation before gonadotoxic treatment, including cryopreservation of oocytes, embryos and ovarian tissue, have therefore been developed. This review focuses on the ovarian function of cancer patients, the safety and efficacy of fertility preservation methods, and the pregnancy outcomes of these patients. Breast cancer and hematological tumors constitute the majority of cancers in reproductive-aged female oncology patients. Ovarian function may not be impacted by breast cancer cells, while in patients with hematological malignancies, decreases in anti-Müllerian hormone and antral follicle counts have been demonstrated. In most cases, patients can undergo ovarian stimulation without delaying treatment, and a new stimulation protocol known as dual stimulation, which may be more efficient, has now been developed. Birth outcomes are also acceptable in cancer patients.

关键词： cancer patients fertility preservation controlled ovarian stimulation safety

Reduction in sperm aneuploidy levels in severe oligoasthenoteratospermic patients after medical therapy： a preliminary report

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Asian Journal of Andrology 2012年第4期14卷 591-598页

作者： Giorgio Cavallini Maria Cristina Magli Andor Crippa Anna Pia Ferraretti Luca Gianaroli Medicitalia Andrological Section Outpatient Clinic of Ferrara Ferrara 44100 Italy laboratory of assisted reproduction Societa Italiana di Medicina della Riproduzione (SISMER) Bologna 40138 Italy Operative Unit of assisted reproduction Societa Italiana di Medicina della Riproduzione (SISMER) Bologna 40138 Italy

The objective of this study was to investigate whether medical therapy can reduce sperm aneuploidy levels and irfiprove the results of intracytoplasmic sperm injection （ICSI） in patients with severe idiopathic oligoasthenoteratospermia （OAT）. Thirty-three infertile couples requiring ICSI because of severe idiopathic OAT after at least one unsuccessful ICSI cycle were considered. Semen parameters （concentration, motility and morphology）, the percentage of aneuploid sperm and the results of ICSI （the number of oocytes fertilized, embryos transferred, biochemical pregnancies, clinical pregnancies and live births） were compared before and after a 3-month course of treatment with L-carnitine 1 g given twice per day＋acetyI-L-carnitine 500 mg given twice per day＋one 30-mg cinnoxicam tablet every 4 days. Aneuploidy was assessed using fluorescent in situ hybridisation （FISH） performed on chromosomes X, Y, 13, 15, 16, 17, 18, 21 and 22. The results showed that 22 of the 33 patients had a reduced frequency of aneuploid sperm and improved sperm morphology after treatment （group 1）, and 11 showed no change （group 2）. The numbers of biochemical pregnancies, clinical pregnancies and live births were significantly higher in group I than in group 2. No significant difference was found between the groups regarding the numbers of oocytes fertilized and embryos transferred. The side effects were negligible. The numbers of ICSI pregnancies and live births in severe idiopathic OAT patients improved with a course of L-carnitine, acetyI-L-carnitine and cinnoxicam.

关键词： acetyI-L-carnitine cinnoxicam ICSl L-carnitine male infertility oligoasthenoteratospermia sperm aneuploidy

APPLICATION OF GENETIC DEAFNESS GENE CHIP FOR DETECTION OF GENE MUTATION OF DEAFNESS IN PREGNANT WOMEN

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Journal of Otology 2014年第2期9卷 97-100页

作者： CHANG Liang ZHONG Su ZHAO Nan LIU Ping ZHAO Yangyu QIAO Jie Department of Obstetrics and Gynecology Peking University Third Hospital Key laboratory of assisted reproduction Ministry of Education Beijing Key laboratory of Reproductive Endocrinology and assisted Reproductive Technology

Objective The study is to identify the carrier rate of common deafness mutation in Chinese pregnant women via detecting deafness gene mutations with gene chip. Methods The pregnant women in obstetric clinic without hearing impairment and hearing disorders family history were selected. The informed consent was signed. Peripheral blood was taken to extract genom- ic DNA. Application of genetic deafness gene chip for detecting 9 mutational hot spot of the most common 4 Chinese deafness genes, namely GJB2 （35delG, 176del16bp, 235delC, 299delAT）, GJB3 （C538T） ,SLC26A4 （ IVS72A〉G, A2168G） and mito- chondrial DNA 12S rRNA （A1555G, C1494T） . Further genetic testing were provided to the spouses and newborns of the screened carriers. Results Peripheral blood of 430 pregnant women were detected, detection of deafness gene mutation carri- ers in 24 cases（4.2%）, including 13 cases of the GJB2 heterozygous mutation, 3 cases of SLC26A4 heterozygous mutation, 1 cases of GJB3 heterozygous mutation, and 1 case of mitochondrial 12S rRNA mutation. 18 spouses and 17 newborns took further genetic tests, and 6 newborns inherited the mutation from their mother. Conclusion The common deafness genes muta- tion has a high carrier rate in pregnant women group, 235delC and IVS7-2A〉G heterozygous mutations are common.

关键词： Gene chip Hereditary deafness Carrier rate Mutation detection

AMP-activated kinase in human spermatozoa： identification, intracellular localization, and key function in the reRulation of sperm motility

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Asian Journal of Andrology 2017年第6期19卷 707-714页

作者： Violeta Calle-Guisado Ana Hurtado de Llera David Martin-Hidalgo Jose Mijares Maria C Gil Ignacio S Alvarez Maria J Bragado Luis J Garcia-Marin Research Group of Intracellular Signalling and Technology of reproduction (SINTREP) School of Veterinary Medicine University of Extremadura Caceres Spain assisted reproduction Unit at the-Minimally Invasive surgery Center Jesos Uson (CCMIJU) Caceres Spain Norba Clinic Caceres Spain Research Group of reproduction and Embryo Development (REDES) University of Extremadura Badajoz Spain Extremadura Institute of assisted reproduction (IERA) Badajoz Spain.

AMP-activated kinase （AMPK）, a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work＇s aims are AMPK identification, intracellular localization, and their role in human spermatozoa function. Semen was obtained from healthy human donors. Sperm AMPK and phospho-Thr172-AMPK were analyzed by Western blotting and indirect immunofluorescence. High- and low-quality sperm populations were separated by a 40%-80% density gradient. Human spermatozoa motility was evaluated by an Integrated Semen Analysis System （ISAS） in the presence or absence of the AMPK inhibitor compound C （CC）. AMPK is localized along the human spermatozoa, at the entire acrosome, midpiece and tail with variable intensity, whereas its active form, phospho-Thr172-AMPK, shows a prominent staining at the acrosome and sperm tail with a weaker staining in the midpiece and the postacrosomal region. Interestingly, spermatozoa bearing an excess residual cytoplasm show strong AMPK staining in this subcellular compartment. Both AMPK and phospho-Thr172-AMPK human spermatozoa contents exhibit important individual variations. Moreover, active AMPK is predominant in the high motility sperm population, where shows a stronger intensity compared with the low motility sperm population. Inhibition of AMPK activity in human spermatozoa by CC treatment leads to a significant reduction in any sperm motility parameter analyzed： percent of motile sperm, sperm velocities, progressivity, and other motility coefficients. This work identifies and points out AMPK as a new molecular mechanism involved in human spermatozoa motility. Further AMPK implications in the clinical efficiency of assisted reproduction and in other reproductive areas need to be studied.

关键词： AMP-activated kinase human spermatozoa immunolocalization sperm motility sperm quality

The Proteome Landscape of Human Placentas for Monochorionic Twins with Selective Intrauterine Growth Restriction

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Genomics, Proteomics & Bioinformatics 2023年第6期21卷 1246-1259页

作者： Xin-Lu Meng Peng-Bo Yuan Xue-Ju Wang Jing Hang Xiao-Ming Shi Yang-Yu Zhao Yuan Wei Department of Obstetrics and Gynecology Peking University Third HospitalBeijing 100191China Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China Key laboratory of assisted reproduction Ministry of EducationBeijing 100191China Beijing Key laboratory of Reproductive Endocrinology and assisted reproduction Beijing 100191China National Clinical Research Center for Obstetrics and Gynecology Beijing 100191China

In perinatal medicine,intrauterine growth restriction(IUGR)is one of the greatest *** etiology of IUGR is multifactorial,but most cases are thought to arise from placental ***,identifying the placental cause of IUGR can be difficult due to numerous confounding *** IUGR(sIUGR)would be a good model to investigate how impaired placentation affects fetal development,as the growth discordance between monochorionic twins cannot be explained by confounding genetic or maternal ***,we constructed and analyzed the placental proteomic profiles of IUGR twins and normal ***,we identified a total of 5481 proteins,of which 233 were differentially expressed(57 up-regulated and 176 down-regulated)in IUGR *** analysis indicates that these differentially expressed proteins(DEPs)are mainly associated with cardiovascular system development and function,organismal survival,and organismal ***,34 DEPs are significantly enriched in angiogenesis,and diminished placental angiogenesis in IUGR twins has been further elaborately ***,we found decreased expression of metadherin(MTDH)in the placentas of IUGR twins and demonstrated that MTDH contributes to placental angiogenesis and fetal growth in ***,our findings reveal the comprehensive proteomic signatures of placentas for sIUGR twins,and the DEPs identified may provide in-depth insights into the pathogenesis of placental dysfunction and subsequent impaired fetal growth.

关键词： Selective intrauterine growth restriction Placenta Proteome Angiogenesis Metadherin