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Analysis of genetic relationship among Arbutus unedo L. genotypes using RAPD and SSR markers

Journal of Forestry Research 2013年第2期24卷 227-236页

作者： Filomena Gomes Rita Costa Maria M. Ribeiro Elisa Figueiredo Jorge M. Canhoto CERNAS Departamento Florestal Escola Superior Agrária Coimbra INRB Instituto Nacional de Recursos Biológicos departamento de Recursos Naturais e desenvolvimento Sustentável Escola Superior Agrária Centre of Functional Ecology Department of Life Sciences University of Coimbra

The strawberry tree （Arbutus unedo L.） is an underutilized, drought tolerant, fire resistant species with a south western distribution in Europe, and with ecological and putative socio-economical impact in Portugal and Mediterranean countries. Our aim was to develop an appropriate set of molecular markers to enable genetic diversity to be assessed and to fingerprint Arbutus unedo genotypes for breeding and conservation purposes in Portugal. Twenty-seven trees from a broad geographic range were screened with 20 random amplified polymorphic DNA （RAPD primers） and 11 microsatellite markers （SSR）. The RAPDs generated 124 bands, 57.3% of which were polymorphic, with an expected heterozygosity of 27%. We cross-amplified 11 SSR primers developed for Vaccinium spp., and 5 were found to be polymorphic in A. unedo, with 75% of expected heterozygosity, a number of alleles of 11.6, a null allele frequency of 7.6% and a polymorphic information content of 71%. Although the SSRs were more polymorphic and informative than the RAPDs, both markers displayed high genetic variability with the gathered data. No geographic pattern was observed in the genetic variation distribution based on both marker systems, and the lack of correlation between genetic and geographical matrices was confirmed by Mantel tests. Likely, no correlation was found between pairwise SSR and RAPD band-sharing matrices. These results and their implications on A. unedo breeding and conservation programs are discussed.

关键词： Ericaceae fingerprinting geographic pattern molecular markers strawberry tree.

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Methodology of Care Humanitude Implementation at an Integrated Continuing Care Unit: Benefits for the Individuals Receiving Care

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Open Journal of Nursing 2020年第10期10卷 960-972页

作者： Liliana Henriques Marília Dourado Rosa Melo João Araújo Mafalda Inácio Instituto Gineste-Marescotti Portugal Via Hominis Leiria Portugal Faculdade de Medicina Universidade de Coimbra Coimbra Portugal escola superior de enfermagem de coimbra Coimbra Portugal Centro de Solidariedade e Cultura de Peniche Leiria Portugal

The increasing number of elderly people with behavioral changes, agitation, aggressiveness, and refusal to receive care imposes new challenges to caregivers, who need to take ownership of innovative care methodologies to better take care of this group of people. Objective: To evaluate the benefits of the Humanitude Care Methodology (HCM) in order to improve the health condition of people admitted in a Portuguese Integrated Continuing Care Unit (UCCI). Methods: An action research study was conducted between September and december 2016. The sample consisted of 33 people who were admitted in this UCCI for at least 90 days. Monthly data collection, during four periods, using the Braden Scale, Morse Scale, Barthel Index and Mini Mental State Examination. The data was processed using the Statistical Package for Social Science, version 17.0. Results: The following decreases were observed: in individuals with cognitive deficit (93.94 percent to 81.82 percent);in individuals considered totally dependent (60.61 percent to 30.30 percent);at risk developing pressure ulcers (PU) (84.85 percent to 51.52 percent);in individuals who had PU (24.24 percent to 15.15 percent);in people with high risk of fall (45.45 percent to 39.39 percent);in the number of individuals with two antipsychotic prescriptions (27.27 percent to 9.09 percent). Conclusions: These results allowed to understand the positive benefits, of implementing the HCM, on the people receiving care, specifically at cognitive level, dependency level in activities of daily living, and the avoidance of physical and mental deterioration.

关键词： Healthcare Quality Humanization of Care Patient-Centered Care Elderly Care dementia

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Mycorrhizal synthesis between Pisolithus arhizus and adult clones of Arbutus unedo in vitro and in nursery

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Journal of Forestry Research 2013年第4期24卷 659-670页

作者： Filomena Gomes Helena Machado Esteban San Martin A.Portugal Jorge M.Canhoto Filomena Gomes.CERNAS Dep.Recursos FlorestaisEscola Superior Agrária Coimbra INIAV Instituto Nacional de Investigao Agráriae VeterináriaIP.Av.República Centre of Functional Ecology Department of Life SciencesUniversity of Coimbra

Arbutoid mycorrhizae were synthesized between adult se- lected clones of Arbutus unedo L. and Pisolithus arhizus. Two micro- propagated clones were tested： ALl, in vitro and C1 （acclimatized plants） in nursery and later in a field trial. In vitro, rooted shoots were trans- ferred to test tubes containing the substrate previously inoculated with mycelium cultured on agar. In the nursery, two inoculation treatments were tested （vegetative inocula or dry sporocarps） and compared to con- trol plants. In the field trial, plants from nursery inoculation treatments were compared and an additional control treatment using seedlings was implemented. Plant height was evaluated 4 months later in the nursery and 20 months later in the field trial.

关键词： arbutoid mycorrhizae Ericaceae field plants Arbutus unedoor strawberry tree

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A multi-scale modelling framework to guide management of plant invasions in a transboundary context

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Forest Ecosystems 2016年第4期3卷 297-310页

作者： Joao Martins David M.Richardson Renato Henriques Elizabete Marchante Hélia Marchante Paulo Alves Mirijam Gaertner Joao P.Honrado Joana R.Vicente InBIO/CIBIO-Centro de Investigacao em Biodiversidade e Recursos Geneticos Universidade do Porto-Campus Agrario de VairaoRua Padre Armando Quintasn°74485-661 VairaoPortugal Centre for Invasion Biology Department of Botany and ZoologyStellenbosch UniversityMatieland 7602South Africa Institute of Earth Sciences(ICT/University of Minho/CCT) MinhoPortugal Centre for Functional Ecology Department of Life SciencesUniversity of Coimbra3000-456 CoimbraPortugal departamento de Ambiente Escola Superior Agraria de CoimbraInstituto Politecnico de CoimbraBencanta3045-601 CoimbraPortugal Faculdade de Ciencias da Universidade do Porto Ediffcio FC4(Biologia)Rua do Campo Alegres/n4169-007 PortoPortugal Environmental Resource Management department Westlake Conservation OfficeOu Kaapse WegTokai7966 City of Cape TownSouth Africa

Background：Attention has recently been drawn to the issue of transboundary invasions,where species introduced and naturalized in one country cross international borders and become problematic in neighbouring *** modelling frameworks,able to identify the environmental drivers of invasion and forecast the current and future potential distribution of invasive species,are needed to study and manage *** due to the lack of species distribution and environmental data,or assumptions of modelling tools,often constrain the reliability of model ***：We present a multiscale spatial modelling framework for transboundary invasions,incorporating robust modelling frameworks（Multimodel Inference and Ensemble Modelling） to overcome some of the *** framework is illustrated using Hakea sericea Schrad.（Proteaceae）,a shrub or small tree native to Australia and invasive in several regions of the world,including the Iberian *** study scales were considered：regional scale（western Iberia,including mainland Portugal and Galicia） and local scale（northwest Portugal）.At the regional scale,the relative importance of environmental predictors sets was evaluated and ranked to determine the main general drivers for the species distribution,while the importance of each environmental predictor was assessed at the local *** potential distribution of *** was spatially projected for both scale ***：Model projections for western Iberia suggest that a large area is environmentally suitable in both Portugal and *** and landscape composition sets were the most important determinants of this regional distribution of the ***,a geological predictor（schist lithology） was more important in explaining its local-scale ***：After being introduced to Portugal,*** has become a transboundary invader by expanding in parts of Galicia（Spain）.The fact that a larger area is predicted as

关键词： Drivers of invasion Hakea sericea Multimodel inference Transboundary invasion management Species distribution models

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The Patient Satisfaction Scale： Validation of a Portuguese Version in Women Receiving Obstetric Care

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Journal of Health Science 2014年第11期2卷 529-537页

作者： Maria Margarida Santana Fialho S im-Sim Maria Joao Pimenta Marques Claudia Alexandra Canaverde Saruga escola superior de enfermagem Universidade de Evora Evora 7000-811 Portugal Servigo de Obstetricia Hospital do Espirito Santo Evora 7000-811 Portugal

Satisfaction of the obstetric patient is an indicator taken in account in health reports. Previous studies point towards several dimensions. In order to better evaluate health care services, it is curtail to have Portuguese language tools of easy appliance. The aim of the study is to validate the PSS in Portuguese. This is a descriptive study using a convenience sample of ninety one mothers. Participants were primary healthcare clients with children aged of I or less. The analysis regarded sensitivity, reliability, as well as concurrent, discriminant, converging and factorial validity. After Promax rotation, three factors were extracted explaining 82.13% of the variance. Reliability analysis was observed by split-half method showing a Spearman-Brown correlation coefficient of 0.91. Cronbach＇s alpha of the Portuguese PSS total scale and subscales were satisfactory with coefficients between 0.829 and 0.941. Concurrent validity was supported by a continuous variable of support from nurses. Discriminant validity showed in women with a normal vaginal delivery, a higher satisfaction both globally and on the three dimensions. The properties observed at the PSS have shown to be satisfactory. PSS reveals itself as a valid measure of the obstetric client＇s satisfaction.

关键词： Nursing obstetric care patient satisfaction instrument development measurement psychometrics.

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Chemical and microbiological characterization of tinctures and microcapsules loaded with Brazilian red propolis extract

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Journal of Pharmaceutical Analysis 2017年第5期7卷 280-287页

作者： Erika Tayse da Cruz Almeida Maria Cristina delgado da Silva José Marcos dos Santos Oliveira Regianne Umeko Kamiya Rodolfo Elleson dos Santos Arruda Danilo Abreu Vieira Valdemir da Costa Silva Pierre Barnabé Escodro Irinaldo Diniz Basílio-Júnior Ticiano Gomes do Nascimento Laboratório de Controle de Qualidade e Análise de Fármacos Medicamentos e AlimentosCurso de FarmáciaEscola de Enfermagem e FarmáciaUniversidade Federal de AlagoasCampus A.C.SimõesBR 104 Norte-Km 97MaceióAL.CEP:57072-970Brazil Laboratório de Controle de Qualidade Microbiológico de Alimentos Faculdade de NutriçãoUniversidade Federal de AlagoasCampus A.C.SimõesBR 104 Norte/Km 97MaceióAL.CEP:57072-970Brazil

The aim of this study was to characterize tinctures and microcapsules loaded with an ethanol extract of red propolis through chemical, physicochemical and microbiological assays in order to establish quality control tools for nutraceutical preparations of red propolis. The markers(isoflavonoids, chalcones, pterocarpans,flavones, phenolic acids, terpenes and guttiferones) present in the tinctures A and B were identified and confirmed using LC/ESI/FTMS/Orbitrap. Four compositions(A, B, C and D) were prepared to contain B tincture of the red propolis with some pharmaceutical excipients and submitted to two drying processes, i. ***-drying and freeze-drying to obtain microcapsules loaded with the red propolis extract. The tinctures and microcapsules of the red propolis were submitted to the total flavonoid content and antioxidant activity *** antibacterial activity and minimum inhibitory concentration(MIC) were tested using Staphylococcus aureus ATCC 25293 and Pseudomonas aeruginosa ATCC 27853 strains. The tinctures and microcapsules presented high flavonoid quantities from 20.50 to 40.79 mg/100 mg of the microcapsules. The antioxidant activity and IC50 were determined for the tinctures A and B(IC50: 6.95 μg/m L and 7.48 μg/m L), the spray-dried microcapsules(IC50: 8.89–15.63 μg/m L) and the freeze-dried microcapsules(IC50: 11.83–23.36 μg/m L). The tinctures and microcapsules were proved to be bioactive against gram-positive and gram-negative bacteria with inhibition halos superior to 10 mm at concentration of 200 μg/m L and MIC values of 135.87–271.74 μg/m L using gram-positive strain and 271.74–543.48 μg/m L using gram-negative strain. The tinctures and microcapsules of the red propolis have a potential application for nutraceutical products.

关键词： Red propolis Tinctures Microcapsules Isoflavonoids LC/ESI/FTMS/Orbitrap Antioxidant activity Antibacterial activity

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SURVEILLANCE ON INTEGRATED PEST MANAGEMENT ON BAIRRADA REGION IN PORTUGAL

SURVEILLANCE ON INTEGRATED PEST MANAGEMENT ON BAIRRADA REGIO...

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The ⅩⅤth International Plant Protection Congress

作者： M.CARDOSO M.NEVES V.BATALHA M.J.CUNHA P.M.MOREIRA escola superior Agrária de coimbra Departamento De Fitotecnia Sector De Proteco Vegetal 3040-316 coimbra Apibairrada -Associao de Proteco E Produo Integrada Da Bairrada Av.Do Cabecinho 3780-203 Anadia escola superior Agrária de coimbra Departamento De Ciências Exactas E Do Ambiente Sector De Matemática E Informática escola superior Agrária de coimbra Departamento De Fitotecnia Sector De Proteco Vegetal 3040-316 coimbra escola superior Agrária de coimbra Departamento De Fitotecnia Sector De Proteco Vegetal 3040-316 coimbra

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Pregnant Women’s Knowledge Gaps about Breastfeeding in Northern Portugal

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Open Journal of Obstetrics and Gynecology 2017年第3期7卷 376-385页

作者： Alexandrina Cardoso Abel Paiva e Silva Heimar Marín escola superior de enfermagem do Porto (Porto School of Nursing) Porto Portugal Federal de S&atilde o Paulo University S&atilde o Paulo Brasil

Introduction: To explore Portuguese pregnant women’ knowledge on breastfeeding and to correlate knowledge scores with the socio-demographic variables. Methods: A cross-sectional design was used to assess 621 pregnant women’ knowledge on breastfeeding at three health centers in the North of Portugal. The face-to-face interviewing was used to collected data using a clinical instrument composed by 18 knowledge breastfeeding descriptors. The reliability of the instrument was very good (KR-20 = 0.88). descriptive and inferential statistics were used to examine pregnant women’ knowledge and the relation with socio-demographics variables. Results: Pregnant women were found to be more knowledgeable in benefits of breastfeeding. In 14 knowledge descriptors more than 60% of pregnant women revealed lack of knowledge. The most usual lack of knowledge concerned how to increase lactation and how to deal with breastfeeding complications. The pregnant women more knowledgeable were the oldest, with higher education, and with previous experience on breastfeeding. Discussion: Pregnant women revealed a significant lack of knowledge to support basic breastfeeding decisions. The focus of care should emphasize on the younger, the first-time mothers and less well-educated women as particular vulnerable and poorer prepared groups concerning breastfeeding knowledge.

关键词： Breastfeeding Nursing-Midwife Assessment Pregnant Women Midwifery

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Multicultural care in nursing—From the theoretical paradigm to the subjective experiences in clinical settings

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Open Journal of Nursing 2013年第8期3卷 557-562页

作者： Ana Paula Teixeira de Almeida Monteiro Aida Cruz Mendes Nursing School of coimbra CoimbraPortugal Unidade de Investigacao em Ciencias da Saude-enfermagem(UICISA-E) CoimbraPortugal

Last decade, the geography of immigration in Portugal experienced intense transformations with an increasing inflow of new and diversified migratory groups. An open questionnaire was developed and applied to 22 Portuguese nurses, aiming to analyse personal experiences and significant situations of nursing care in multicultural contexts. The understanding of hospital routines, difficulties in managing physical space due to different cultural standards, body exposure, and body handling in nursing clinical care, emerged as more significant issues in reports of critical incidents focusing on multicultural contexts.

关键词： Multicultural Nursing Mental Health and Culture

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Epidemiological Characteristics, Resistance Patterns and Spread of Gram-Negative Bacteria Related to Colonization of Patients in Intensive Care Units

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Advances in Infectious Diseases 2015年第1期5卷 14-20页

作者： Quésia Souza Damaceno Jacques Nicoli Adriana Cristina Oliveira departamento de enfermagem Básica Escola de Enfermagem da Universidade Federal de Minas Gerais Belo Horizonte Brazil departamento de Microbiologia Universidade Federal de Minas Gerais Belo Horizonte Brazil

Our aim was to determine the epidemiological characteristics, the resistance patterns and the spread of Gram negative bacteria related to colonization of patients in adult Intensive Care Units. Methods: A prospective cohort of patients colonized and/or infected with Gram negative bacteria was conducted at two adult ICUs from hospitals in Brazil (April 2012 to February 2013). Nasal, groin and perineum swabs were performed. Samples were incubated on MacConkey and cetrimide agar (48 h at 37℃) and identification tests (Vitek-BioMérieux), antibiogram (Bauer-Kirby method), Carba NP test, Polymerase Chain Reaction (PCR) and sequencing were performed. The patterns of resistant microorganisms were compared by rep-PCR (Diversilab). Results: There were 53 cases of colonization. In these cases, we identified imipenem-resistant Acinetobacter baumannii (51%), Pseudomonas aeruginosa (32%), Klebsiella pneumoniae ESBL (38%) or imipenem resistant (5.6%). The use of antimicrobials and medical devices were related to colonization (p The resistance patterns expressed by Klebsiella pneumoniae were ESBL (CTX-M, SHV e TEM) and KPC2. A verified profile of Acinetobacter baumannii was related to OXA-23 and OXA-253 (OXA-143 variant). The profiles ESBL and KPC2 expressed by Klebsiella pneumoniae were distributed between the both ICUs. The distribution of OXA-23 and OXA-253 was verified only in one ICU. The similarity of strains ranged from 80% to 95%, highlighting the horizontal transference of these microorganisms.

关键词： Intensive Care Unit Bacterial Drug-Resistance Gram-Negative Aerobic Bacteria Colonization

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