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De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze theβ-poly(L-malic acid)biosynthesis pathway under the CaCO_(3) addition

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food Science and Human Wellness 2023年第4期12卷 1248-1256页

作者： Genan Wang Haisong Yin Tingbin Zhao Donglin Yang Shiru Jia Changsheng Qiao Key Laboratory of Industrial Fermentation Microbiology(Tianjin University of Science and Technology) Ministry of EducationTianjin 300457China Tianjin engineering research center of Microbial Metabolism and Fermentation Process Control College of BiotechnologyTianjin University of Science and TechnologyTianjin 300457China School of Bioengineering Tianjin Modern Vocational Technology CollegeTianjin 300350China Tianjin Huizhi Biotrans Bioengineering Co. Ltd.Tianjin 300457China

β-Poly(L-malic acid)(PMLA)is a water-soluble biopolymer used in food,medicine and other *** date,the biosynthesis pathway of PMLA has not been fully *** this study,we sequenced the transcriptom e of strain Aureobasidium melanogenum under 20 g/L CaCO_(3) *** resulting sequencing reads were assembled and annotated for the differentially expressed genes(DEGs)analysis and novel transcripts *** result indicated that with the CaCO_(3) addition,the tricarboxylic cycle(TCA)cycle and glyoxylate pathway were up-regulated,and it also found that a non-ribosomal peptide synthetase(NRPS)like protein was highly *** DEGs analysis showed a high expression level of malate dehydrogenase(MDHC)and phosphoenolpyruvate carboxykinase(PCKA)in the CaCO_(3) group,which indicated a cytosolic malate *** speculated that the malate should be transported to or synthesized in the cytoplasm,which was then polymerized to PMLA by the NRPS-like protein,accompanied by the up-regulated TCA cycle providing ATP for the *** on the analysis,we assumed that an NRPS-like protein,the TCA cycle,and the cytosolic malate together are contributing to the PMLA biosynthesis.

关键词： De novo transcriptome analysis β-Poly(L-malic acid) Aureobasidium melanogenum

engineering yeast for bio-production of food ingredients

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Systems Microbiology and Biomanufacturing 2023年第1期3卷 2-11页

作者： Chunyang Cao Jiaoqi Gao Beiwei Zhu Yongjin J.Zhou Division of biotechnology Dalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian 116023People’s Republic of China National engineering research center of Seafood Collaborative Innovation Center of Seafood Deep ProcessingLiaoning Province Collaborative Innovation Center for Marine Food Deep ProcessingSchool of Food Science and TechnologyDalian Polytechnic UniversityDalian 116034People’s Republic of China

The provision of an adequate and high-quality food supply is a challenging issue due to the continued growth of the population and the reduction of arable land *** solve these problems,new and efficient food manufacturing processes need to be developed to meet the needs for healthy and nutritious *** engineering of microorganisms is a feasible approach to alleviate this problem due to its efficient *** thousands of years,yeast has been used as a cell factory for manufacturing bread,beer and *** the development of synthetic biology expands its ability for synthesis of food ingredients,fuels,pharmaceuticals and chemical *** mini review focuses on metabolic engineering of yeast cell factories to synthesize compounds that have been used as food ingredients with highlighting four food flavors.

关键词： Yeast Bio-products Metabolic engineering food ingredients Cell factory

Enhanced cobalamin biosynthesis in Ensifer adhaerens by regulation of key genes with gradient promoters

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synthetic and Systems biotechnology 2022年第3期7卷 941-948页

作者： Sha Xu Zhiqiang Xiao Shiqin Yu Weizhu Zeng Yongming Zhu Jingwen Zhou National engineering Laboratory for Cereal Fermentation Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China School of biotechnology and Key Laboratory of Industrial biotechnology Ministry of EducationJiangnan University1800 Lihu RoadWuxiJiangsu214122China Science center for Future foods Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Provisional research center for Bioactive Product Processing Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Hunan Hongying biotechnology Co.Ltd. 10 Hongying RoadJinshiHunan415400China

Cobalamin is an essential human vitamin widely used in the pharmaceutical,food,and feed additive industries and currently produced by bacteria or *** adhaerens HY-1 is an industrial strain that also produces *** production outputs are poor and the specific synthesis pathways require *** this study,the whole genome sequence of *** HY-1 was generated and annotated,and genes associated with cobalamin biosynthesis were ***,three genes,CobSV,CobQ,and CobW were identified as the most efficient ones for enhancing cobalamin *** transcriptome sequencing of *** HY-1 cells at different growth stages,65 endogenous promoters with different gradient strengths were *** combined expression of different strength promoters and key genes,a high cobalamin-producing recombinant strain,‘hmm’(genotype:PmetH-CobSV-PibpA-CobQ-Pmdh-CobW),was *** production was 143.8 mg/L in shaking flasks,which was 41.0%higher than the original *** production was further enhanced to 171.2 mg/L using fed-batch ***,our data and novel approach provide important references for the analysis of cobalamin synthesis and other metabolites in complex metabolic pathways.

关键词： Ensifer adhaerens Cobalamin Genome sequence Transcriptome sequence Promoter Overexpression Combinations

Transcriptomics reveals the effect of ammonia nitrogen concentration on Pseudomonas stutzeri F2 assimilation and the analysis of amtB function

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synthetic and Systems biotechnology 2023年第2期8卷 262-272页

作者： Wei-lai Fu Pei-feng Duan Qiang Wang Ya-xin Liao Yun-shuang Wang Mei-juan Xu Hui-hui Jiang Xian Zhang Zhi-ming Rao The Key Laboratory of Industrial biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122PR China The Key Laboratory of Functional Aquafeed and Culture Environment Control Fujian DBN Huayou Aquatic Science and Technology Co.LtdZhangzhou363500PR China School of Biological and Environmental engineering Chaohu UniversityHefei238024PR China

The biological treatment of wastewater with high concentrations of ammonia nitrogen has become a hot research issue,but there are limited reports on the mechanism of ammonia nitrogen utilization by *** this paper,a transcriptomic approach was used to investigate the differences in gene expression at 500.0 mg/L(Amo 500)and 100.0 mg/L(Amo 100)ammonium concentrations to reveal the mechanism of ammonia nitrogen removal from water by Pseudomonas stutzeri *** transcriptome data showed 1015(459 up-regulated and 556 down-regulated)differentially expressed genes with functional gene annotation related to nitrogen source metabolism,glycolysis,tricarboxylic acid cycle,extracellular polysaccharide synthesis,energy conversion and transmembrane transport,revealing the metabolic process of ammonium nitrogen conversion to biological ni-trogen in *** F2 through *** verify the effect of ammonium transporter protein(AmtB)of cell membrane on assimilation,a *** F2-ΔamtB mutant strain was obtained by constructing a knockout plasmid(pK18mobsacB-ΔamtB),and it was found that the growth characteristics and ammonium removal rate of the mutant strain were significantly reduced at high ammonium *** carbon source components and dissolved oxygen conditions were optimized after analyzing the transcriptome data,and the ammonium removal rate was increased from 41.23%to 94.92%with 500.0 mg/L ammonium *** study of *** F2 transcript level reveals the mechanism of ammonia nitrogen influence on microbial assimilation process and improvement strategy,which provides a new strategy for the treatment of ammonia nitrogen wastewater.

关键词： Ammonia nitrogen Pseudomonas stutzeri F2 Transcriptomic Assimilation amtB

Screening of phenolic compounds in australian grown grapes and their potential antioxidant activities

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food Bioscience 2022年第3期47卷 544-556页

作者： Gia Toan Vo Ziyao Liu Osbert Chou Biming Zhong Colin J.Barrow Frank R.Dunshea Hafiz A.R.Suleria School of Agriculture and food Faculty of Veterinary and Agricultural SciencesThe University of MelbourneParkvilleVIC3010Australia CAS Key Laboratory of Quantitative engineering Biology Synthetic Biochemistry CenterShenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China Centre for Chemistry and biotechnology School of Life and Environmental SciencesDeakin UniversityWaurn PondsVIC3217Australia Faculty of Biological Sciences The University of LeedsLeedsLS2 9JTUK

Grapes(Vitis Vinifera)are one of the world's most utilised and prized *** are a great source of antioxidants due to the abundance of phenolic *** pulp is the main part of the grape being *** pulp of five Australian grown grapes–Dawn Seedless(DS),Calmeria(CG),Golden Globe(GG),Menindee Seedless(MS),and Thompson Seedless(TS)will be qualitatively and quantitatively analysed for its phenolic composition and antioxidant *** general trend followed:GG>TS>MS>DS>CG,and the phenolic content of the pulp also presented a significant positive correlation with the antioxidant *** revealing the highest levels of TPC(4.31±0.11 mg GAE/g)and TFC(0.48±0.09 mg QE/g)while TS were highest in TTC(0.94±0.12 mg CE/g).Similarly,GG exhibited higher antioxidant capacity in DPPH(5.84±0.17 mg AAE/g),FRAP(4.91±0.14 mg AAE/g),and TAC(6.78±0.12 mg AAE/g),while TS revealed highest radical scavenging ability for ABTS(4.56±0.19 mg AAE/g).The LC-ESI-QTOF-MS/MS tentatively identified 78 phenolic compounds consisting of flavonoids(36),phenolic acids(31),lignans(3),stilbenes(1),and other polyphenols(7)in five grapes *** most abundant fruit-based targeted polyphenols were *** had a higher phenolic concentration in HPLC-PDA quantification,particularly for phenolic acids(gallic acid and chlorogenic acid)and flavonoids(kaempferol).Our study provides relatively adequate information on grape pulp's phenolic content and antioxidant capacity,providing confidence and research prospects to promote grape pulp use in different food,pharmaceutical and supplement industries,and even influencing grape cultivation.

关键词： Australian grown grapes Phenolic compounds LC-ESI-QTOF-MS/MS Antioxidant activity HPLC-PDA

Stability of potentially toxic elements in municipal sludge biochars modified by MgCl2 and phosphate

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Waste Disposal and Sustainable Energy 2023年第1期5卷 13-23页

作者： Qiqi Huang Siqi Chen Jinhao Lin Jingzi Beiyuan Jin Wang Juan Liu Yundang Wu Xiaolian Wu Fuhua Li Wenbing Yuan Chengrong Nie School of Environmental and Chemical engineering Foshan UniversityFoshan528000China School of food Science and Technology Foshan UniversityFoshan528000China Foshan engineering and Technology research center for Contaminated Soil Remediation School of Environmental and Chemical EngineeringFoshan UniversityFoshan528000China School of Environmental Science and engineering Key Laboratory of Water Quality and Conservation in the Pearl River DeltaMinistry of EducationGuangzhou UniversityGuangzhou510006China Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management Institute of Eco-Environmental and Soil SciencesGuangdong Academy of SciencesNational-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South ChinaGuangzhou510650China

Municipal wastewater sludge can be pyrolyzed as biochars to better use nutrients and stabilize carbon compared with other typical technologies,such as landfill and ***,sludge-derived biochars might contain large amounts of potentially toxic elements(PTEs),such as Zn,Cu,Cr,Ni,Pb,and *** stability of PTEs in biochars might be improved by higher pyrolytic temperatures,which can be further improved by different ***,PO4-modification at 300°C and Cl-modification at 700°C were carried out,respectively,to enhance the stability of *** leaching tests have been performed to assess the stability of PTEs in biochars,including the synthetic precipitation leaching procedure(SPLP),toxicity characteristic leaching procedure(TCLP),diethylenetriamine pentaacetate(DTPA)extraction,and in vitro simple bioaccessibility extraction test(SBET).The morphological structure,elemental mapping,and mineral formation of the pristine and modified biochars were studied by scanning electron microscopy–energy-dispersive X-ray spectroscopy(SEM–EDS)and X-ray diffraction(XRD).Our results suggested that the leachability,mobility,plant-availability,and bioaccessibility of most PTEs were decreased by pyrolysis,yet the total contents of PTEs were elevated,especially at 700°***,modification by phosphates and MgCl2 enhanced the stability of PTEs in ***,it should be noted that higher bioaccessibility of PTEs was observed in biochars of P-modification than Cl-modification,which is associated with the dissolution of phosphate precipitates under acidic conditions(pH<2).Additionally,Cl-modification leads to higher plant-available Zn and Cu and bioaccessible Zn compared with the unmodified biochar produced at 700°C.

关键词： Biochar modification Sludge biochar Sewage sludge utilization Chlorination Pyrolysis Heavy metals

biotechnology ofα-linolenic acid in oilseed rape(Brassica napus)using FAD2 and FAD3 from chia(Salvia hispanica)

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Journal of Integrative Agriculture 2023年第12期22卷 3810-3815页

作者： XUE Yu-fei Inkabanga Tseke ALAIN YIN Neng-wen JIANG Jia-yi ZHAO Yan-ping LU Kun LI Jia-na DING Yan-song ZHANG Shi-qing CHAI You-rong Integrative Science center of Germplasm Creation in Western China(Chongqing)Science City and Southwest University/Chongqing Key Laboratory of Crop Quality Improvement/College of Agronomy and biotechnology Southwest UniversityChongqing 400715P.R.China engineering research center of South Upland Agriculture Ministry of Education/Academy of Agricultural SciencesSouthwest UniversityChongqing 400715P.R.China Faculty of Agricultural Sciences National Pedagogic University(UPN)Kinshasa 8815D.R.Congo

α-Linolenic acid(ALA,18:3Δ9,12,15)is an essential fatty acid for humans since it is the precursor for the biosynthesis of omega-3 long-chain polyunsaturated fatty acids(LC-PUFA).Modern people generally suffer from deficiency of ALA because most staple food oils are low or lack ALA *** enrichment of ALA in staple oil crops is a promising ***(Salvia hispanica)has the highest ALA content in its seed oil among known oil *** this study,the FAD2 and FAD3 genes from chia were engineered into a staple oil crop,oilseed rape(Brassica napus),via Agrobaterium tumefaciens-mediated transformation of their LP4-2A fusion gene construct driven by the seed-specific promoter P_(NapA).In seeds of T0,T1,and T2 lines,the average ALA contents were 20.86,23.54,and 24.92%,respectively,which were 2.21,2.68,and 3.03 folds of the non-transformed controls(9.42,8.78,and 8.22%),*** highest seed ALA levels of T0,T1,and T2 plants were 38.41,35.98,and 39.19%respectively,which were 4.10-4.77 folds of the respective ***-pathway enzyme genes(BnACCD,BnFATA,BnSAD,BnSCD,BnDGAT1,BnDGAT2,and BnDGAT3)and positive regulatory genes(BnWRI1,BnLEC1,BnL1L,BnLEC2,BnABI3,BnbZIP67,and BnMYB96)were all significantly *** contrast,BnTT1,BnTT2,BnTT8,BnTT16,BnTTG1,and BnTTG2,encoding negative oil accumulation regulators but positive secondary metabolism regulators,were all significantly *** means the foreign ShFAD2-ShFAD3 fusion gene,directly and indirectly,remodeled both positive and negative loci of the whole FA-related network in transgenic *** seeds.

关键词： biotechnology α-linolenic acid oilseed rape(Brassica napus) FAD2 FAD3 chia(Salvia hispanica)

Bio-Review 2021: Inspiring the future of Biochemical engineering

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Chinese Journal of Chemical engineering 2021年第2期34卷 1-3页

作者： Yan Sun Department of Biochemical engineering School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology(Ministry of Education)Tianjin UniversityTianjin 300350China

Biochemical engineering(BCE)discipline had largely developed from fermentation technology studies from 1950s through 1960s when fermentation emerged as the core technology able to address multiple industrial needs ranging from health care products such as antibiotics;food products such as single cell proteins,amino acids,organic acids,and vitamins;liquid fuels and chemicals such as ethanol and acetic acid;and industrial enzymes.

关键词： acetic ethanol vitamin

Microbial synthesis of bacitracin:Recent progress,challenges,and prospects

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synthetic and Systems biotechnology 2023年第2期8卷 314-322页

作者： Jiang Zhu Shiyi Wang Cheng Wang Zhi Wang Gan Luo Junhui Li Yangyang Zhan Dongbo Cai Shouwen Chen State Key Laboratory of Biocatalysis and Enzyme engineering Environmental Microbial Technology Center of Hubei ProvinceCollege of Life SciencesHubei UniversityWuhan430062PR China Hubei Provincial Key Laboratory of Industrial Microbiology Key Laboratory of Fermentation Engineering(Ministry of Education)School of Food and Biological EngineeringHubei University of TechnologyWuhan430068HubeiPR China Lifecome Biochemistry Co.Ltd Nanping353400PR China

Microorganisms are important sources of various natural products that have been commercialized for human medicine and animal *** is an important antibacterial natural product predominantly pro-duced by Bacillus licheniformis and Bacillus subtilis,and it is characterized by a broad antimicrobial spectrum,strong activity and low resistance,thus bacitracin is extensively applied in animal feed and veterinary medicine *** recent years,various strategies have been proposed to improve bacitracin ***,we systematically describe the regulation of bacitracin biosynthesis in genus Bacillus and its associated mechanism,to provide a theoretical basis for bacitracin *** metabolic engineering strategies applied for bacitracin production are explored,including improving substrate utilization,using an enlarged precursor amino acid pool,increasing ATP supply and NADPH generation,and engineering transcription *** also present several approaches of fermentation process optimization to facilitate the industrial large-scale production of ***,the challenges and prospects associated with microbial bacitracin synthesis are discussed to facilitate the establishment of high-yield and low-cost biological factories.

关键词： Bacillus Bacitracin Metabolic regulation Metabolic engineering Fermentation process