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Arylamine N-acetyltransferases: a new inhibitor of apoptosis in HepG2 cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第9期9卷 701-706页

作者： Yu-bin JI1,2, Shi-yong GAO1,2 (1Postdoctoral research Station of the Institute of Materia Medica, center for Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China) (2MOE engineering research center of Natural anticancer drugs, Harbin University of Commerce, Harbin 150076, China) Postdoctoral research Station of the Institute of Materia Medica Center for Life Sciences and Environmental Sciences Harbin University of Commerce Harbin 150076 China MOE engineering research center of Natural anticancer drugs Harbin University

Objective: To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis. Methods: NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry. Results: NAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells. Conclusion: NAT can inhibit apoptosis in HepG2 cells.

关键词： Arylamine N-acetyltransferases (NATs) Apoptosis HepG2 Camptothecin (CAM)

Antihepatocarcinoma Effect of Solanine and Its Mechanisms

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Chinese Herbal Medicines 2012年第2期4卷 126-135页

作者： Yu-bin JI[a][b] Shi-yong GAO[a][b] center for Life Sciences and Environmental Sciences Harbin University of Commerce Harbin 150076 China MOE engineering research center of Natural anticancer drugs Harbin University of Commerce Harbin 150076 China Center for Life Sciences and Environmental Sciences Harbin University of Commerce Harbin 150076 China MOE engineering research center of Natural anticancer drugs Harbin University of Commerce Harbin 150076 China

Objective To explore the antitumor effect of solanine and its *** The in vivo antitumor effect of solanine was observed using models developed through in vivo transplantation of tumor cells;In vitro lines of sensitive antitumor cells were selected from the digestive system using MTT assay;The effect of solanine on cell morphology was observed using transmission electronic microscopy;The morphology of apoptotic cells was observed using Annexin V/PI double staining and laser confocal scanning microscopy(LCSM);The rate of cell apoptosis was measured using Annexin V/PI double staining and flow cytometry;The concentration of intracellular Ca 2+ ([Ca 2+ ]i)was determined using Fluo-3/AM staining and LCSM;The membrane potential of cellular mitochondria was determined using TMRE staining and LCSM;The protein expression of Bcl-2 and Bax was measured using immunological marking and LCSM;And the activity of caspase-3 was measured using the colorimetric *** Solanine could inhibit the growth of tumor weight in S180 tumor-bearing mice and prolong the survival time of H22 tumor-bearing *** assay revealed that HepG2 cells were quite sensitive to solanine because solanine could induce morphological changes in HepG2 cells,with the rate of early apoptosis being 4%,8.5%,and 20.1%,for HepG2 cells treated for 24 h with solanine at concentration of 0.4,2,and 10μg/mL, *** could raise the[Ca 2+ ]i and lower the membrane *** could reduce the protein expression of Bcl-2 while increase that of Bax,thus increasing the activity of *** The obvious antitumor activity of solanine in human hepatocarcinoma is *** inhibitory effect is achieved through solanine decreasing the Bcl-2/Bax ratio,thus increasing[Ca 2+ ]i,which could enhance the enzymatic activity of the caspase family,thus inducing the apoptosis of HepG2 cells.

关键词： apoptosis caspase hepatocarcinoma S180 solanine

Application of in situ/operando characterization techniques in heterostructure catalysts toward water electrolysis

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Nano research 2023年第2期16卷 1984-1991页

作者： Wanyi Liao Shanshan Wang Hong Su Yan Zhang Sichuan engineering research center for Biomimetic Synthesis of Natural drugs School of Life Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China

The key to achieving the breakthrough of hydrogen energy from marginal energy sources in large scale applications lies in the development and design of efficient electrocatalysts for the electrochemical oxidation and reduction of *** unique heterostructure endows the catalyst with a mass of functional interfaces that are decisive for the enhancement of catalyst activity,stability,and reaction *** some cutting-edge reviews have focused on the synthesis strategies,constitution,and applications of heterostructure catalysts,the field still lacks a detailed discussion of the actual reaction processes occurring at the interface,which is detrimental to the understanding of the true catalytic *** on advanced in situ/operando characterization techniques to understand the working mechanism of heterostructure catalysts is essential for rational design of advanced *** this review,we first present the advantages of heterostructure catalysts applied to electrolyzing ***,the application of in situ/operando techniques in probing three aspects of heterostructure catalyst surface reconstruction,reaction mechanism,and the role of each component is highlighted with classical case ***,the current challenges and prospects for the design of heterostructure electrocatalysts are discussed in detail.

关键词： in situ/operando characterization heterostructure reconfiguration reaction mechanism electrochemical water splitting

Material Base of in vivo Invigorating Qi and Enriching Blood of Bazhen Decoction by HPLC-ESI-MS

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Chinese Herbal Medicines 2012年第4期4卷 282-286页

作者： LI Wen-lan BAI Jing SUN Zhi DU Juan JI Yu-bin center of research on Life Sciences and Environmental Sciences Harbin University of Commerce Harbin 150076 China engineering research center of Natural anticancer drugs Ministry of Education Harbin University of Commerce Harbin 150076 China

Objective To identify the in vivo metabolized chemical constituents in Bazhen Decoction(BZD) and to study the relationship between Siwu Decoction(SWD) and Sijunzi Decoction(SJZD) with *** Analysis and comparison were carried out by *** samples after ig administration of preparations such as SWD,SJZD,and BZD,with different ingredients were collected for *** Twenty-two components were detected after ig administration of BZD,and six of them were metabolites and others were original form of the components contained in *** prototype constituents were atractylenolide I,5-hydroxymethyl-furoic acid(5-HMFA),oxypaeoniflorin,atractylenolide III,albiflorin,paeoniflorin,liquiritin,ferulic acid,ligustilide,and ginsenosides Rg1 and *** metabolized constituents were paeonimetabolin I,glycyrrhetic acid monoglucuronide,glycyrrhetinic acid,and ginsenosides Rh1 and *** The results also show that 5-HMFA,oxypaeoniflorin,albiflorin,paeoniflorin,ferulic acid,and ligustilide are common components absorbed into blood existing in both BZD and SWD,which are material base of enriching blood;Atractylenolide I,atractylenolide III,and liquiritin are common ingredients absorbed into blood existing in both BZD and SJZD,and they are material base of invigorating *** results provide basic data for the further studies on the effective components,the effecting mechanism,and the quality control of BZD.

关键词： Bazhen Decoction HPLC-ESI-MS in vivo metabolites prototype components

Diverse strategic approaches en route to Taxol total synthesis

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Chinese Chemical Letters 2022年第12期33卷 4957-4968页

作者： Zexian Li Jianfeng Zheng Wei-Dong Z.Li Sichuan engineering research center for Biomimetic Synthesis of Natural drugs School of Life Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China

Taxol is one of the most famous diterpenoid natural products used in clinical cancer *** and its analogues are popular synthetic targets and have attracted worldwide attention over the past *** research efforts have already been made and ten groups have achieved the total synthesis of Taxol since *** mini-review summarized the recent highlights of divergent strategic approaches towards the chemical synthesis of Taxol's carbocyclic framework bearing a bridged eight-membered ring.

关键词： Taxol Total synthesis Biomimetic synthesis Linear strategy Convergent strategy

Genome editing of PAR2 through targeted delivery of CRISPR-Cas9 system for alleviating acute lung inflammation via ERK/NLRP3/IL-1β and NO/iNOS signalling

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1441-1456页

作者： Xin Zhuo Yue Wu Xiujuan Fu Jianbin Li Yuxin Xiang Xiaoyu Liang Canquan Mao Yuhong Jiang Sichuan engineering research center for Biomimetic Synthesis of Natural drugs School of Life Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China

Excessive and uncontrollable inflammatory responses in alveoli can dramatically exacerbate pulmonary disease progressions through vigorous cytokine releases,immune cell infiltration and protease-driven tissue *** is an urgent need to explore potential drug strategies for mitigating lung ***-activated receptor 2(PAR2)as a vital molecular target principally participates in various inflammatory diseases via intracellular signal ***,it has been rarely reported about the role of PAR2 in lung *** study applied CRISPR-Cas9 system encoding Cas9 and sg RNA(p Cas9-PAR2)for PAR2 knockout and fabricated an anionic human serum albuminbased nanoparticles to deliver p Cas9-PAR2 with superior inflammation-targeting efficiency and stability(TAP/p Cas9-PAR2).TAP/p Cas9-PAR2 robustly facilitated p Cas9-PAR2 to enter and transfect inflammatory cells,eliciting precise gene editing of PAR2 in vitro and in ***,PAR2 deficiency by TAP/p Cas9-PAR2 effectively and safely promoted macrophage polarization,suppressed proinflammatory cytokine releases and alleviated acute lung inflammation,uncovering a novel value of *** also revealed that PAR2-mediated pulmonary inflammation prevented by TAP/p Cas9-PAR2was mainly dependent on ERK-mediated NLRP3/IL-1β and NO/i NOS ***,this work indicated PAR2 as a novel target for lung inflammation and provided a potential nanodrug strategy for PAR2 deficiency in treating inflammatory diseases.

关键词： Protease-activated receptor 2(PAR2) CRISPR-Cas9 Gene editing Inflammation Acute lung inflammation NLRP3 Nanoparticles Drug delivery

Tumor inflammation-specific self-amplifying fluorescent molecular probes induce an anti-tumor immune response

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Science China Chemistry 2025年第1期 273-287页

作者： Shuguang Yang Lijun Hu Hong Xiao Zecong Xiao Xintao Shuai PCFM Lab of Ministry of Education School of Materials Science and Engineering Sun Yat-sen University Department of Ultrasound The Fifth Affiliated Hospital Sun Yat-sen University Department of Medical Ultrasound The Third Affiliated Hospital of Sun Yat-sen University Nanomedicine research center The Third Affiliated Hospital of Sun Yat-sen University

Enhancing the concentration of exogenous molecular drugs within the tumor microenvironment through enzyme-catalyzed polymerization presents a novel strategy for cancer therapy. Nonetheless, the optimization of the catalytic efficiency is often impeded by the inefficient expression of enzymes. Herein, we reported a self-amplifying fluorescent molecular probe, Bis-HTPICG, for photodynamic therapy(PDT) and subsequent PDT-induced immunoreaction. The Bis-HTP-ICG probe possesses a noticeable enzyme-catalyzed polymerization facilitated by myeloperoxidase(MPO), a crucial enzyme secreted by neutrophils at inflammation sites. Upon exposure to laser irradiation, Bis-HTP-ICG showed a high PDT efficacy, inducing an acute inflammatory response that stimulates further recruitment of neutrophils and then elevated MPO secretion. The heightened level of MPO enhances the accumulation of the Bis-HTP-ICG via self-polymerization or binding with intratumoral proteins following MPO enzyme catalysis, instigating a self-amplifying chain reaction cycle involving Bis-HTP-ICG, neutrophils and ***, PDT efficiently incites immunogenic cell death(ICD) in tumor cells, initiating an anti-tumor immune response including dendritic cells(DCs) maturation, T cell proliferation and reprogramming of tumor-associated neutrophils(TANs). This work portrays a promising strategy for self-amplification of fluorescent molecular probes through adjustable enzyme levels,potentially offering a unique avenue to enhance the tumor accumulation of molecular drugs for improved tumor therapy.

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Terbium-doped zinc oxide constructed dual-light-responsive nitric oxide-releasing platform for bacterial keratitis treatment

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Nano research 2023年第1期16卷 849-857页

作者： Yue Sun Wei Zhang Menghui Wang Huijie Liu Qun Li Jiazhe Luo Mengzhen Zhao Shichen Liu Xiaolei Wang School of Chemistry and Chemical engineering Nanchang UniversityNanchang 330088China The National engineering research center for Bioengineering drugs and the Technologies Institute of Translational MedicineNanchang UniversityNanchang 330088China

Bacterial keratitis(BK)is a principal factor contributing to corneal ulcers and ocular complications,and has become a significant killer of global vision *** antibiotic therapy has unsatisfactory therapeutic effect due to short residence time and low utilization rate of drug,and the long-term abuse of antibiotics can easily incur the prevalence of drug-resistant ***,antibiotics are not effective in addressing the excessive inflammatory response caused by ***,we developed a terbium-doped zinc oxide constructed dual-light-responsive nitric oxide(NO)-releasing nanoparticle(ZT@P@B)and modified it on the surface of contact lenses(CL-ZT@P@B)to boost its *** vitro and in vivo experimental results verified that ZT@P@B could simultaneously respond to green light(GL)and 808 nm near-infrared(NIR)without obvious damage to the eyes,releasing reactive oxygen species(ROS)and NO,which could effectively kill bacteria(including drug-resistant strains)and reduce inflammatory *** platform provides a promising strategy for the prevention and treatment of BK.

关键词： zinc oxide nitric oxide dual-light-responsive bacterial keratitis contact lenses

Pan-cancer immunogenic death analysis identifies key roles of CXCR3 and CCL18 in hepatocellular carcinoma

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Genes & Diseases 2024年第2期11卷 568-570页

作者： Jia Guo Xin Sun Guowei Pan Ce Li Xin He Xiaofang Che Zan Teng Xiujuan Qu Yunpeng Liu Bowen Yang Department of Medical Oncology Provincial Key Laboratory of Anticancer Drugs and Biotherapy of Liaoning ProvinceLiaoning Provincial Clinical Research Center for CancerClinical Cancer Research Center of ShenyangThe First Hospital of China Medical UniversityShenyangLiaoning 110001China research center for Universal Health School of Public HealthChina Medical UniversityShenyangLiaoning 110122China

Immunogenic death is a form of programmed cell death that is common in the development and progression of ***,its impact on tumor progression differs depending on the location of the ***,it also plays an important role in antitumor immunity because the process of immunogenic death can release substances that activate immunity.1 Therefore,in this study,the key mechanism of mediating immunogenic death in hepatocellular carcinoma(HCC)was found from the excavation of the immunogenic death characteristics of pan-cancer and the in-depth investigation of its effects on different tumor types(Fig.1A).

关键词： death CXCR3 immunity