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Cloning of Immune System-related Gene ApTOLL1 and Its Expression in Nosema-infected Antheraea pernyi

Agricultural biotechnology 2012年第3期1卷 49-51页

作者： Lijun WANG Jinshan XU Zeyang ZHOU Chongqing Key Laboratory of Animal Biology Chongqing Normal University active substances biotechnology research center of Ministry of Education Chongqing Normal University Institute of Sericulture and Systems Biology Southwestern University

[ Objective ] This study aimed to identify the immune system-related TOLL-like receptor family gene of Aherea pernyi, to lay the foundation for further investigating the immune mechanism of Antherea perny/. [ Method] Immune system-related TOLL-like receptor family gene of Antherea perny/was cloned for se- quencing and phylogenetic analysis ; in addition, expression variations of TOLL-like receptor family gene in Antherea pernyi infected with Nosema pernyi and Nosema bombycis were detected to analyze the differences in immunological reactivity of Antherea pernyi to Nosema infection. [ Result] Based on cDNA cloning and sequen- cing, an immune system-related gene fragment was isolated from Antherea pernyi, which is the most homologous to the ToUl gene in Toll signaling pathway of Bom- byx mot/according to the sequencing result and phylogenetic analysis, which is named ApTolll gene. Subsequently, Antherea pernyi pupae were injected respective- ly with Nosema bombycis and Nosema pernyi, fluorescent real-time quantitative PCR analysis showed that ApTolll was abundantly expressed in Antherea pernyi pupa 2 h after Nosema bombycis injection, which began to express in Antherea pernyi pupa 11 h after Nosema pernyi injection, indicating that the immune response time induced by various Nosema strains varies in Toll signaling pathway of Antherea perny/. [ Conclusion ] ApTolll gene of Antherea perny/was first cloned in this study, which provides reference for further investigating the immune mechanism of Antherea pernyi.

关键词： Antheraea pernyi Nosema TOLL-like receptor protein Immune defense

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Identification of microRNA-like RNAs in Ophiocordyceps sinensis

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Science China(Life Sciences) 2019年第3期62卷 349-356页

作者： Wen Zhang Xiaona Li Lina Ma Uzair Urrehman Xilinqiqige Bao Yujing Zhang Chen-Yu Zhang Dongxia Hou Zhen Zhou State Key Laboratory of Pharmaceutical biotechnology Jiangsu Engineering Research Center for microRNA Biology and Biotechnology NJU Advanced Institute for Life Sciences (NAILS) School of Life Sciences Nanjing University The Drug Laboratory of Mongolian Medicine and Pharmacy Institute Inner Mongolia International Mongolian Hospital

Ophiocordyceps sinensis is well known as a traditional Chinese medicine and has widely been used for over 2,000 years to stimulate immune system, decrease blood pressure and to inhibit tumor growth. While miRNAs are increasingly recognized for their roles in post-transcriptional regulation of gene expression in animals and plants, miRNAs in fungi were less studied until the discovery of microRNA-like RNA(milRNA). High-throughput sequencing and bioinformatics approaches were used to identify conserved and novel milRNAs in O. sinensis. 40 conserved milRNAs were identified, while 23 pre-miRNA candidates encoding 31 novel milRNAs were predicted. Furthermore, the potential target genes of milRNAs in human were predicted and gene ontology analysis was applied to these genes. Enrichment analysis of GO-represented biological process showed that target genes of both conserved and novel milRNAs are involved in development, metabolic and immune processes, indicating the potential roles of milRNAs of O. sinensis in pharmacological effects as health food and traditional Chinese medicine. This study is the first report on genome-wide analysis of milRNAs in O. sinensis and it provides a useful resource to further study the potential roles of milRNAs as active components of O. sinensis in health food or traditional Chinese medicine.

关键词： Ophiocordyceps sinensis O.sinensis miRNA milRNA high-throughput sequencing

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Green Electrospun Silk Fibroin Nanofibers Loaded with Cationic Ethosomes for Transdermal Drug Delivery

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Chemical research in Chinese Universities 2021年第3期37卷 488-495页

作者： HONG Huoyan ZHANG Dongdong UN Si HAN Feng WANG Kaili JIANG Di WU Jinglei MO Xiumei WANG Hongsheng Key Laboratory of Science&Technology of Eco-Textile Ministry of EducationShanghai Engineering Research Center of Nano-Biomaterials and Regenerative MedicineCollege of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghai201620P.R.China

Transdermal drug delivery system(TDDS)facilitates the controlled release of active ingredients penetrating through the skin,avoiding the liver first pass *** is a simple process to fabricate ultrafine fibers with a higher specific surface area,making them excellent candidates for drug *** current work,a novel silk fibroin(SF)nanofiber loaded with cationic ethosomes(CEs)was prepared via green *** data of Fourier transform infrared spectroscopy(FTIR)and laser scanning confocal microscopy(LSCM)confirmed the existence of CEs in the SF *** morphology of the nanofibers was not significantly affected by the incorporation of CEs as shown by scanning electron microscopy(SEM)*** CEs-loaded SF nanofibrous patch(CEs-SFnP)showed good cytocompatibility as proved by both cell counting Kit-8(CCK-8)assay and *** doxorubicin hydrochloride(Dox)as a model drug,the transdermal performance of CEs-SFnP was evaluated through Franz diffusion cell against mouse *** results indicated that CEs-SFnP can effectively deliver drug into the skin,with a much higher permeation rate than the normal nanofibers without *** as-spun CEs-SFnP in this study could find promising applications in TDDS.

关键词： Transdermal drug delivery Silk fibroin Ethosome Green electrospinning

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Antioxidant antileishmanial cytotoxic and antimicrobial activities of a local plant Myrtus nivellei from Algeria Sahara

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Asian Pacific Journal of Tropical Biomedicine 2017年第8期7卷 702-707页

作者： Farah Ramdane Rym Essid Nadia Fares Dahmane El Ouassis Sana Aziz Mahfoud Hadj Mahammed Mohamed Didi Ould Hadj Ferid Limam Biogeochemistry Laboratory in Desert Environments Kasdi Merbah University Faculty of Natural and Life Sciences El Oued University Laboratory of Bioactive substances Biotechnology Center of Borj-Cedria Technopole National Institute of Forest research Protection Laboratory of Ecosystem in Arid and Semi Arid Zone Kasdi Merbah University

To study the phytochemical constituents and in vitro biological activities of hydromethanolic extract and fractions from Algerian Sahara Myrtus nivellei (M. nivellei) collected in Hoggar region and to identify the active fraction that can act as an alternative of commonly used antibiotics and as antileishmanial or antioxidant *** screening of M. nivellei aerial parts was realised according to the literature. Extract was firstly prepared by using aqueous methanol then fractionated with ethyl acetate and butanol solvents. Total phenolics, tannis and flavonoids, of the hydromethanolic extract and their fractions were determined by Folin-Ciocalteu method as gallic acid equivalents and by aluminium chloride as rutin equivalent respectively. Extract and fractions were tested for their antimicrobial and antiparasital activities against standard bacteria using agar diffusion method and two kinds of leishmania visceral and cutaneous. The antioxidant activities were realized using phosphomolybdenum, FRAP and DPPH *** phytochemical screening exhibited the presence of flavonoids, tannins, saponins, and alkaloids. The experimental results showed that plant extract and fractions were high in phenolic compounds and exhibited an important role as antioxidant, antimicrobial and had a moderate antileishmanial *** observations lead us toward more studies in this field, so that we can get more benefits from our local Algerian medicinal plants.

关键词： Phenolic compounds Flavonoids Antioxidant activity Antimicrobial activity Antileishmanial activity Myrtus nivellei

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Echinacoside and Cistanche tubulosa(Schenk) R. wight have male reproductive protection effect by targeting hypothalamic and rogen receptor

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中国药理学与毒理学杂志 2018年第4期32卷 257-258页

作者： Xiao-ying ZHANG Zhi-hui JIANG Xue-mei CHANG Rui-min CAO College of Veterinary Medicine Northwest A&F University research center of Modern biotechnology School of Biotechnology and Food EngineeringAnyang Institute of Technology College of Veterinary Medicine Xinjiang Agricultural University

OBJECTIVE Cistanche deserticola *** is an official plant that grows in arid or semi-arid *** early work demonstrated that Cistanche extracts protect against sperm damage in mice under bisphenol A induced reproductive ***(ECH) is one of the major active *** present study investigated the mechanisms behind the possible protective effects of ECH against oligoasthenospermia in rat and identified the interaction between ECH and *** The distribution of ECH was assayed by HPLC,the quantity and quality of sperm was evaluated and hormone levels were determined by radioimmunosorbent *** levels of androgen receptor(AR) and key steroidogenic-related genes were reduced as determined by Western blotting and qPCR *** interaction between ECH and AR were evaluated by fluorescence localization assay,indirect ELISA and molecular *** ECH significantly increased the quantity of sperm and secretions of luteinizing hormone and *** was distributed to the hypothalamus but not in the *** combined with hypothalamic AR in the pocket of Met-894 and Val-713 to inhibit transfer of AR from the cytoplasm to nuclei in the *** negative feedback of sex hormone *** was inhibited,positive feedback was stimulated to increase the secretion of luteinizing hormone and testosterone subsequently enhancing the quantity of *** significantly alleviated the BPA-induced reproductive damage by increasing testicular superoxide dismutase(SOD),glutathione peroxidase(GSH-PX) and glutathione(GSH);as well as by reducing serum malondialdehyde(MDA).*** not only obviously enhanced the levels of serumLH and T,but it also improved the sperm count and motility compared to the BPA-treated *** *** and ECH protect the BPA induced reproductive *** blocks AR activity in the hypothalamus to increase the quantity of sperm and protect against oligoasthenospermia in rats.

关键词：肉苁蓉植物类固醇药物分析

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Regulating the nanoscale intimacy of metal and acidic sites in Ru/γ-Al_(2)O_(3)for the selective conversions of lignin-derived phenols to jet fuels

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Journal of Energy Chemistry 2022年第3期31卷 576-586,I0015页

作者： Shanshuai Chen Weichen Wang Xue Li Puxiang Yan Wanying Han Tian Sheng Tiansheng Deng Wanbin Zhu Hongliang Wang center of Biomass engineering/College of Agronomy and biotechnology China Agricultural UniversityBeijing 100193China Sanya Institute of China Agricultural University Sanya 572025HainanChina College of Chemistry and Materials Science Anhui Normal UniversityWuhu 241000AnhuiChina Shanxi engineering research center of Biorefinery Institute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001ShanxiChina

Catalytic hydrodeoxygenation(HDO)of biomass-derived oxy-compounds to advanced hydrocarbon fuels usually requires bifunctional catalysts containing metals and acidic *** appropriate tuning of metal and/or acidic active sites at interfaces of bifunctional catalysts can significantly improve catalyst activity and product ***,4-trifuoromethyl salicylic acid(TFMSA),as a hydrothermal stable organic acid,was employed to tailor the bifunctional interface of Ru/γ-Al_(2)O_(3)to enhance the catalytic performance on converting lignin-derived phenols to jet fuel range *** than 80%phenol was converted into cyclohexane at 230°C for 1 h over Ru/γ-Al_(2)O_(3)modified by TFMSA,which was about three times higher than that over unmodified Ru/γ-Al_(2)O_(3).X-ray diffraction(XRD),Transmission electron microscope(TEM),H2 chemisorption,and energy dispersive X-ray spectroscopy(EDS)elemental mapping results indicated that Ru nanoparticles and TFMSA were well distributed onγ-Al_(2)O_(3),and a nanoscale intimacy between Ru and TFMSA was ***,Fourier transform infrared spectroscopy after pyridine adsorption(Py-FT-IR)analysis proved that Brønsted acidic sites on the catalytic interfaces of TFMSA modified Ru/γ-Al_(2)O_(3)had been ***,the kinetic and density functional theory(DFT)results suggested that the synergistic effects of adjacent Ru nanoparticles and acidic sites were crutial for promoting the rate-limiting conversion step of phenol HDO to cyclohexane.

关键词： Lignin Phenols Bifunctional catalysts Hydrodeoxygenation Jet fuels

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Antioxidant effect of Lactobacillus fermentum HFY02-fermented soy milk on D-galactose-induced aging mouse model

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Food Science and Human Wellness 2022年第5期11卷 1362-1372页

作者： Tiantian Hu Rui Chen Yu Qian Ke Ye Xingyao Long Kun-Young Park Xin Zhao Chongqing Collaborative Innovation center for Functional Food Chongqing Engineering Research Center of Functional FoodChongqing Engineering Laboratory for Research and Development of Functional FoodChongqing University of EducationChongqing 400067China Department of General Practice The Third People's Hospital of ChengduChengdu 610036China Department of Food Science and biotechnology Cha UniversitySeongnam 13488Korea

This study aimed to investigate the antioxidant effect of soybean milk fermented by a new type of Lactobacillus fermentum(LF-HFY02)by using D-galactose induced aging mice ***,the optimal fermentation conditions was screened out by detecting the effects of different fermentation temperature and time on the active components and antioxidant activity of soybean milk in *** then unfermented soybean milk and the soybean milk fermented by different Lactobacillus was given by gavage to D-galactose-induced aging *** activities of GSH,GSH-Px,SOD,CAT and T-AOC in serum,brain and liver of soybean milk fermented by LF-HFY02 were significantly increased,while the content of MDA and the level of AGEs in hippocampal were significantly decreased compared with D-galactose induced *** more,the mRNA expression of GSH and SOD in mouse liver were obviously up-regulated by soybean milk fermented by *** skin tissue structure of mice in the LF-HFY02 fermented soybean milk group was more complete,the collagen fibers were increased and arranged orderly and liver inflammation has improved compared with the model *** Western blot analysis showed that LF-HFY02 effectively upregulated EGFR,SOD and GSH protein expression in mouse *** findings suggest that LF-HFY02 can effectively prevent D-galactose-induced oxidation and aging in mice,and the effect was even better than that of the Lactobacillus delbruechii *** and vitamin ***,LF-HFY02 may be potentially employed as a probiotic *** conclusion,soybean milk fermented by LF-HFY02 can increase the content of antioxidant factors and the activity of antioxidant enzymes by regulating gene and protein expression,and finally inhibit the process of tissue cell peroxidation,and improve the oxidative damage of mouse skin and *** results could provide a basis for the research and development and industrial production of probiotic-related fermented soybean milk products.

关键词： Lactobacillus fermentum HFY02 Fermented soybean milk D-galactose Antioxidant Aging

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GC-MS analysis and anti-mosquito activities of Juniperus virginiana essential oil against Anopheles stephensi(Diptera: Culicidae)

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Asian Pacific Journal of Tropical Biomedicine 2019年第4期9卷 168-175页

作者： Azar Tahghighi Naseh Maleki-Ravasan Navid Dinparast Djadid Hamzeh Alipour Raziyeh Ahmadvand Fateh Karimian Saeed Yousefinejad Department of Clinical research Pasteur Institute of Iran Malaria and Vector research Group Biotechnology Research Center Pasteur Institute of Iran Department of Parasitology Pasteur Institute of Iran research center for Health Sciences Institute of Health Shiraz University of Medical Sciences Department of Medical Entomology and Vector Control School of Public health Tehran University of Medical sciences research center for Health Sciences Institute of Health Department of Occupational Health Engineering School of Health Shiraz University of Medical Sciences

Objective: To investigate phytochemicals present in the essential oil from aerial parts of eastern red cedar, Juniperus virginiana(J. virginiana) L.(Cupressaceae) and to determine its killing and repellent activities against larvae, pupae, and adults of the Asian malaria mosquito, Anopheles stephensi(Diptera: Culicidae). Methods: J. virginiana essential oil was extracted by hydrodistillation, and its chemical composition was determined by gas chromatography-mass spectrometry. Seven different logarithmic concentrations of J. virginiana essential oils were used in larvicidal and pupicidal assays. J. virginiana essential oils-impregnated bed nets were applied in a designed animal module to test excito-repellent activity against adult mosquitoes. Results: Fourteen constituents corresponding to 99.98% of J. virginiana essential oils were identified. Five main components were terpinen-4-ol(25.21%), camphor(19.89%), E-3-hexen-1-ol(13.30%), γ-terpinene(7.86%), and l-menthone(2.27%). The LC_(50) and LC_(90) values against larvae of the Anopheles stephensi were 11.693 and 66.140 ppm and for pupae were 9.640 and 40.976 ppm, respectively. In excito-repellency assay, J. virginiana essential oilsimpregnated bed nets provided an average of 54.63% protection for guinea pig and 45.37% mortality for the mosquitoes. Conclusions: Four monoterpenes and one leaf alcohol were identified by gas chromatographymass spectrometry. J. virginiana essential oils showed potent larvicidal, pupicidal, adulticidal, and repellent activities against Anopheles stephensi at acceptable concentrations. Evaluation of bioactivity of identified chemicals(alone or in combination) will provide new eco-friendly substances for mosquito-management programs.

关键词： Malaria Pesticide Essential oils Natural products Resistance

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research on Extraction Process of Gallic Acid from <i>Penthorum chinense</i>Pursh by Aqueous Ethanol

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Green and Sustainable Chemistry 2015年第2期5卷 63-69页

作者： Luping Kang Shanbin Yang Yan Peng Jiao Dai Xingchun Ying Pharmaceutical engineering Institute of engineering research center of biotechnology of active Materials (Ministry of Education) in Chongqing Normal University Chongqing China Key Laboratory of Green Synthesis and Applications College of Chemistry Chongqing Normal University Chongqing China

Penthorum chinense Pursh is rich in gallic acid, which has antioxidant, anti-inflammatory, anti-fungal and antitumor activities. In order to optimize their extraction conditions, various extraction parameters were chosen to identify their effects on gallic acid extraction. With extraction amount of gallic acid as index, based on single factor analysis, influence of solid/liquid ratio, ethanol concentration, fetch time and extraction temperature on extraction technology were investigated by orthogonal test. The optimization conditions for gallic acid extraction were determined as follows: ethanol concentration 60%, extraction time 2.5 h, temperature 90°C and solid/liquid ratio 1:30. The corresponding gallic acid content was 4.85%. This optimized extraction process was stable and feasible.

关键词： Penthorum Gallic Acid Extraction Process Orthogonal Design

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Preparation and Characterization of IgY from the Egg Yolk of Hens Immunized with the Venom of Deinagkistrodonacutus

Preparation and Characterization of IgY from the Egg Yolk of...

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第12届生物毒素研究及医药应用学术大会

作者： Qiyi He Wenwen Wang Gang Liu HailongDuan Xian Yang Xiaodong Yu Animal Toxin Group Chongqing Key Laboratory of Animal BiologyChongqing Engineering Research Center of Bioactive SubstanceEngineering Research Center of Active Substance and BiotechnologyMinistry of EducationCollege of Life ScienceChongqing Normal Univrsity

Deinagkistrodonacutus caused a large number of snakebite incidents related to human in southern *** intravenous administration of conventional antivenoms produced in horses or other mammal is the mainstay and the only specific treatment of *** antibodies（IgY）,isolated from chicken egg yolks,are regarded as a new alternative to be applied for the snake *** on the reasons,we produced IgY antibodies against *** venom and assessed its protective *** our studies,two 24-week-old laying white leghorn chickens were immunized intramuscularly in the breast region at multiple sites with 0.293 mg of *** venoms to generate IgY antibodies against the venom *** the first immunization,Chickens received three boost at 21 days apart to raise the level of specific antibodies in egg *** being extracted by caprylic acid and ammonium sulphate precipitation,the crude extracts of antibodies were further purified by affinity *** purity and molecular weight of IgY antibodies was determined by SDS-PAGE and the specificity of antibodies was studied by immunodiffusion *** ELISA and Western blot analysis were used to determine the titer and antigen recognition repertoire of IgY antibodies *** median effective dose（ED50） of anti-Deinagkistrodonacutusvenoms IgY antibodies was 14.14 mg IgY/ kg body weight with the challenge dose as 3LD50.150 ug of IgY antibodies was able to neutralized 50%of 3MHD of *** results indicate that antibodies generated in chicken may probably to be a new star which is superior alternative then conventional immunoglobulins in respect of the treatment for snake envenomation and in the future.

关键词： IgY egg yolk snake venom Deinagkistrodonacutus LD50

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