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Journal of Integrative Plant Biology 2016年第6期58卷 577-589页

作者： Guang-Hui Xiao Kun Wang Gai Huang Yu-Xian Zhu The State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Beijing 100871 China Institute for Advanced Studies/College of life Sciences Wuhan University Wuhan 430072 China College of life Sciences Shaanxi Normal University Xi'an 710062 China

Production of b-ketoacyl-Co A, which is catalyzed by 3-ketoacyl-CoA synthase（KCS）, is the first step in very long chain fatty acid（VLCFA） biosynthesis. Here we identified 58 KCS genes from Gossypium hirsutum, 31 from G. arboreum and 33 from G. raimondii by searching the assembled cotton genomes. The gene family was divided into the plant-specific FAE1-type and the more general elo-type. KCS transcripts were widely expressed and 32 of them showed distinct subgenome-specific expressions in one or more cotton tissues/organs studied. Six Gh KCS genes rescued the lethality of elo2Δelo3Δ yeast double mutant,indicating that this gene family possesses diversified *** KCS genes with GA-responsive elements（GAREs） in the promoters were significantly upregulated by gibberellin A_3（GA）.Exogenous GA_3 not only promoted fiber length, but also increased the thickness of cell walls significantly. GAREs present also in the promoters of several cellulose synthase（CesA） genes required for cell wall biosynthesis and they were all induced significantly by GA_3. Because GA treatment resulted in longer cotton fibers with thicker cell walls and higher dry weight per unit cell length, we suggest that it may regulate fiber elongation upstream of the VLCFA-ethylene pathway and also in the downstream steps towards cell wall synthesis.

关键词： 3-Ketoacyl-CoA synthase cotton expression patterns gibberellins very long chain fatty acids

<i>In Situ</i>Hydroxyapatite Synthesis: Influence of Collagen on Its Structural and Morphological Characteristic

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Materials Sciences and Applications 2012年第8期3卷 580-586页

作者： Luis C. Mendes Geysy L. Ribeiro Raphaella C. Marques Instituto de Macromoléculas Professora eloísa Mano (IMA) Universidade Federal do Rio de Janeiro (UFRJ) Rio de Janeiro Brasil

Hydroxyapatite (HA) was synthesized in situ by the precipitation method, with and without the presence of collagen (COLL), to study its influence on HA’s structural and morphological characteristics. The material was characterized by energy dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), wide-angle X-ray diffraction (WAXD), thermogravimetric analysis (TG) and scanning electron microscopy (SEM). The Ca/P molar ratio was influenced by collagen addition—1.89 and 2.38 for samples without and with collagen, respectively. The WAXD pattern revealed better resolution and intensity besides higher crystallinity degree of the HA in the presence of collagen. The photomicrographs showed a strong influence of collagen on the HA morphology.

关键词： Hydroxyapatite Collagen In Situ Precipitation Method Morphology

Strawberry sweetness and consumer preference are enhanced by specific volatile compounds

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Horticulture Research 2021年第1期8卷 870-884页

作者： Zhen Fan Tomas Hasing Timothy S.Johnson Drake M.Garner Michael L.Schwieterman Christopher R.Barbey Thomas A.Colquhoun Charles A.Sims Marcio F.R.Resende Vance M.Whitaker Horticultural Sciences Department University of FloridaIFAS Gulf Coast Research and Education CenterWimaumaFLUSA elo life systems DurhamNCUSA Department of Environmental Horticulture and Plant Innovation Center Institute of Food and Agricultural SciencesUniversity of FloridaGainesvilleFLUSA Plenty Unlimited Inc.South San FranciscoCA 94080USA Food Science and Human Nutrition Department University of FloridaGainesvilleFLUSA Horticultural Sciences Department University of FloridaGainesvilleFLUSA

Breeding crops for improved flavor is challenging due to the high cost of sensory evaluation and the difficulty of connecting sensory experience to chemical *** main goal of this study was to identify the chemical drivers of sweetness and consumer liking for fresh strawberries(Fragaria×ananassa).Fruit of 148 strawberry samples from cultivars and breeding selections were grown and harvested over seven years and were subjected to both sensory and chemical *** panel consisted of at least 100 consumers,resulting in more than 15,000 sensory data points per *** sugars,two acids and 113 volatile compounds were *** liking was highly associated with sweetness intensity,texture liking,and flavor intensity,but not sourness *** least square analyses revealed 20 volatile compounds that increased sweetness perception independently of sugars;18 volatiles that increased liking independently of sugars;and 15 volatile compounds that had positive effects on *** learning-based predictive models including sugars,acids,and volatiles explained at least 25%more variation in sweetness and liking than models accounting for sugars and acids *** compounds such asγ-dodecalactone;5-hepten-2-one,6-methyl;and multiple medium-chain fatty acid esters may serve as targets for breeding or quality control attributes for strawberry products.A genetic association study identified two loci controlling ester production,both on linkage group 6 ***-segregating makers in these regions can be used for increasing multiple esters *** study demonstrates a paradigm for improvement of fruit sweetness and flavor in which consumers drive the identification of the most important chemical targets,which in turn drives the discovery of genetic targets for marker-assisted breeding.

关键词： volatile sugar esters

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声学脉冲识别技术进入触摸屏市场

现代显示 2007年第4期 17-21页

作者： Ken North Henry D'Souza 孙婕石琳 elo触摸系统公司北京科技大学

声学脉冲识别(APR)技术,一种新的弯曲波触摸屏技术,利用“声学指纹识别”法在触摸屏上对触摸位置更精准地定位。

关键词：声学脉冲识别技术弯曲波触摸屏技术声学指纹识别

The Development and Characterization of Polycaprolactone and Titanium Dioxide Hybrids

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Advances in Nanoparticles 2018年第1期7卷 11-27页

作者： Mariana Sato de S. B. Monteiro Maria Inês Bruno Tavares Faculdade de Farmácia Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil Instituto de Macromoléculas Professora eloísa Mano Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil

Organic/Inorganic hybrid materials have been attracting much attention since they combine the advantages of inorganic materials with the properties of organic polymers. Titanium dioxide nanoparticles (TiO2) present good thermal stability, accessibility and catalytic properties. Polycaprolactone (PCL) is a bi-ocompatible and bioresorbable material, which is being examined as biode-gradable packaging materials, controlled drug release carriers and other medical applications. Hybrids based on PCL containing different amounts of titanium dioxide nanoparticles, ranging from 0.05% to 0.35% w/w, were prepared using the solution cast method. These systems were characterized by X-ray diffraction (XRD), infrared spectroscopy (FTIR), low-field nuclear magnetic resonance (NMR), thermogravimetric analysis (TG) and differential scanning calorimetry (DSC). The FTIR analysis confirmed that there was an interaction between the PCL chains and the TiO2 nanoparticles. The XRD and DSC analysis showed that the PCL crystallization was affected by TiO2 incorporation, modifying its semi-crystalline structure to a less ordered structure. When TiO2 nanoparticles were added the values of T1H and T1ρH increased for all hybrids, therefore, their addition produced a new material with less molecular mobility. In the TG analysis, it was observed that the introduction of TiO2 nanoparticles decreased the thermal resistance of PCL. In DSC analysis, the PCL/TiO2 hybrids presented a reduction in the crystallization temperature and degree of crystallinity, except for PCL hybrids containing 0.15% w/w of TiO2 nanoparticles.

关键词： Poly (ε -Caprolactone) Dioxide Titanium Hybrids NMR Relaxometry

Identification of KCS gene family and functional analysis of FAE-like genes from Malania oleifera

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Oil Crop Science 2021年第1期6卷 35-40页

作者： Huan Dai Yanyu Zhang Pei Jin Fengping Song Wei Xu Aizhong Liu Zuqing Meng Tianquan Yang Department of Plant Science Tibet Agriculture and Animal Husbandy CollegeLinzhi860000China College of life Science Northwest UniversityXi’an710069ShaanxiChina Southwest Forestry University Kunming650224China Kunming Institute of Botany Chinese Academy of SciencesKunming650201China

KCS(3-ketoacyl-CoA synthase)is the key enzyme catalyzing the first step of very long chain fatty acid(VLCFA)*** showed that different KCSs possessed different substrate *** oleifera are abundance of VLCFAs in its mature seeds,especially the nervonic acid,which is essential for human *** this study,we identified and characterized 18 KCS genes in *** *** analysis showed that these KCS genes were classified into four subfamilies,including two FAE-like,six KCS-like,eight FDH-like and two *** concentrated on the functional role of two FAE-like genes,Maole003085.T1 and Maole004215.T1 which encoded predicted amino acid residues of 516 and 518 in protein,*** sequence alignment showed that their two proteins contained the known and conserved active sites among FAE-like *** heterologous expression in wild type yeast(Saccharomyces cerevisiae)INVSc1,we found that Maole004215.T1 could produce four new fatty acids including C22:0/C22:1 and C24:0/C24:1,but Maole003085.T1 only produced C22:***,upon heterologous expression in mutant yeast BY4741-△elo3,we found the Maole003085.T1 could produce C24:0 and C26:0,while the Maole004215.T1 could catalyze the formation of fatty acids C24:0,C26:0 and C28:*** results showed Maole003085.T1 and Maole004215.T1 had fatty acid elongation activity in yeast,and possessed different substrate preference in the production of different ***,we found Maole004215.T1 could produce nervonic acid in yeast,which provides molecular basis on the genetic improvement and genic engineering for producing nervonic acid resources by using biotechnological methods.

关键词： Malania oleifera KCS gene Very long chain fatty acid Nervonic acid

Correction: Strawberry sweetness and consumer preference are enhanced by specific volatile compounds

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Horticulture Research 2021年第1期8卷 3067-3067页

作者： Zhen Fan Tomas Hasing Timothy S.Johnson Drake M.Garner Michael L.Schwieterman Christopher R.Barbey Thomas A.Colquhoun Charles A.Sims Marcio F.R.Resende Vance M.Whitaker Horticultural Sciences Department University of Florida IFAS Gulf Coast Research and Education Center Wimauma FL USA elo life systems Durham NC USA Department of Environmental Horticulture and Plant Innovation Center Institute of Food and Agricultural Sciences University of Florida Gainesville FL USA Plenty Unlimited Inc. South San Francisco CA 94080 USA Food Science and Human Nutrition Department University of Florida Gainesville FL USA Horticultural Sciences Department University of Florida Gainesville FL USA

Since the publication of this article 1,the corresponding author discovered that an additional researcher contributed data and should be included as an *** ***(Plenty Unlimited,Inc.,South San Francisco,CA 94080,US)has... 详细信息

关键词： volatile specific compounds

Evaluation of Nevirapine Release Kinetics from Polycaprolactone Hybrids

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Materials Sciences and Applications 2016年第11期7卷 680-701页

作者： Mariana S. S. B. Monteiro Jackeline Lunz Pedro J. Sebastião Maria Inês Bruno Tavares Faculdade de Farmácia Universidade Federal do Rio de Janeiro Centro de Ciências da Saúde Bloco L Cidade Universitária Ilha do Fund&atilde o Rio de Janeiro Brazil Instituto de Macromoléculas Professora eloísa Mano Universidade Federal do Rio de Janeiro Centro de Tecnologia Bloco J Cidade Universitária Ilha do Fund&atilde o Rio de Janeiro Brazil Departamento de Física Instituto Superior Técnico Technical University of Lisbon Lisbon Portugal

The Nevirapine (NVP)/Polycaprolactone (PCL)/Nanoparticles hybrids systems have been developed as a potential platform for drug delivery, by solvent cast, as thin films. NVP, an antiretroviral drug, was included within PCL matrix containing three types of nanoparticles: an organoclay layered silicate Viscogel S7®(3% w/w), hydrophilic silica oxide particles Aerosil®A20 (0.25% w/w) and titanium dioxide particles (0.25% w/w). These systems were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), low-field nuclear magnetic resonance (NMR), ultraviolet-visible spectroscopy (UV), in-vitro dissolution testing and drug release mechanism kinetics. The PCL crystallization was affected by NVP incorporation, modifying its semi-crystalline structure to a less ordered structure. When nanoparticles and NVP were added, the T1H values increased, for PCL, PCL/S7, PCL/ SiO2 and PCL/TiO2 hybrids, suggesting that its addition produced a new material, with less molecular mobility, due to the new intermolecular interactions formation. It can consider a structure formation among the PCL chains, nanoparticles and NVP, with strong forces in the PCL/SiO2/NVP system. The amount of NVP included was around 1.5 ± 0.03 mg/cm2. In the in-vitro dissolution test, the PCL/SiO2/NVP system released the smallest amount of drug and this result could be attributed to the strong intermolecular interaction between the drug and the PCL/SiO2 system. Higuchi’s model was the mathematical model chosen to treat the release data, since this model presented the highest coefficient correlation (r) value. The drug release probably occur by diffusion through the matrix pores, thus, these materials are suitable for sustained release of NVP.

关键词： Polycaprolactone Nevirapine Hybrids Modified Release

Effect of Graphene Nanoplatelets Presence on the Thermal and Mechanical Properties of Polypropylene Fibers Produced by Melt Spinning

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材料科学与工程（中英文B版） 2020年第2期10卷 53-63页

作者： Ricardo Rodrigo Ramos Cecci Adriano Alves Passos Nathan Riany Valério Albino Daniel da Silva Vicente Ademir Severino Duarte Maria Inês Bruno Tavares Instituto de Macromoléculas Professora eloísa Mano Universidade Federal do Rio de JaneiroRio de Janeiro 21941-598Brazil SENAI Innovation Institute for Biosynthetic and Fibers SENAI CETIQTRio de Janeiro 20961-020Brazil

In aiming to obtain fibers with enhanced thermal and mechanical properties,graphene based textile fibers with 144 filaments were developed using an approach in which the PP/GnP(polypropylene/graphene nanoplatelets)nanocomposite was employed as conductive material in a fiber with circular cross-section *** kinetics of thermal degradation was evaluated by the Broido method using thermogravimetric analysis(TGA).Activation energy was enhanced from 260.6 kJ·mol^-1 to 337.4 kJ·mol^-1 compared to the neat *** increased the thermal stability of the PP,slowing its degradation by thermal ***,the degree of crystallization declined as the GnP content increased,reducing the tenacity of the yarn,but improving its elastic modulus from 91.9 to 95.9 cN/tex,being a promising alternative to produce smart *** conclusion,it has been confirmed that GnP loading up to 1%(w/w)can be incorporated into polypropylene by melt spinning and that the resulting nanocomposite fibers are suitable for several applications in the textile industry.

关键词： Polymer fibers polypropylene graphene nanoplatelets degradation kinetics

Global Wheat Head Detection(GWHD)Dataset:A Large and Diverse Dataset of High-Resolution RGB-Labelled Images to Develop and Benchmark Wheat Head Detection Methods

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Plant Phenomics 2020年第1期2卷 243-254页

作者： Etienne David Simon Madec Pouria Sadeghi-Tehran Helge Aasen Bangyou Zheng Shouyang Liu Norbert Kirchgessner Goro Ishikawa Koichi Nagasawa Minhajul A.Badhon Curtis Pozniak Benoit de Solan Andreas Hund Scott C.Chapman Frédéric Baret Ian Stavness Wei Guo Arvalis Institut du végétal3 Rue Joseph et Marie Hackin75116 ParisFrance UMR1114 EMMAH INRAECentre PACABâtiment ClimatDomaine Saint-Paul228 Route de l’AérodromeCS 4050984914 Avignon CedexFrance Plant Sciences Department Rothamsted ResearchHarpendenUK Institute of Agricultural Sciences ETH ZurichUniversitätstrasse 28092 ZurichSwitzerland CSIRO Agriculture and Food Queensland Biosciences Precinct306 Carmody RoadSt Lucia4067 QLDAustralia Plant Phenomics Research Center Nanjing Agricultural UniversityNanjingChina Institute of Crop Science National Agriculture and Food Research OrganizationJapan Hokkaido Agricultural Research Center National Agriculture and Food Research OrganizationJapan Department of Computer Science University of SaskatchewanCanada Department of Plant Sciences University of SaskatchewanCanada School of Food and Agricultural Sciences The University of QueenslandGatton4343 QLDAustralia Graduate School of Agricultural and life Sciences The University of Tokyo1-1-1 Midori-choNishitokyo CityTokyoJapan

The detection of wheat heads in plant images is an important task for estimating pertinent wheat traits including head population density and head characteristics such as health,size,maturity stage,and the presence of *** studies have developed methods for wheat head detection from high-resolution RGB imagery based on machine learning ***,these methods have generally been calibrated and validated on limited *** variability in observational conditions,genotypic differences,development stages,and head orientation makes wheat head detection a challenge for computer ***,possible blurring due to motion or wind and overlap between heads for dense populations make this task even more *** a joint international collaborative effort,we have built a large,diverse,and well-labelled dataset of wheat images,called the Global Wheat Head Detection(GWHD)*** contains 4700 high-resolution RGB images and 190000 labelled wheat heads collected from several countries around the world at different growth stages with a wide range of *** for image acquisition,associating minimum metadata to respect FAIR principles,and consistent head labelling methods are proposed when developing new head detection *** GWHD dataset is publicly available at http://***/and aimed at developing and benchmarking methods for wheat head detection.

关键词： Wheat wheat maturity