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Stem cell heterogeneity and regenerative competence:the enormous potential of rare cells

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Neural Regeneration research 2021年第2期16卷 285-286页

作者： Emily A.B.Gilbert Cindi M.Morshead Terrence donnelly centre for cellular and biomolecular research Division of AnatomyDepartment of SurgeryUniversity of TorontoTorontoONCanada Institute of Biomedical and Biochemical Engineering Institute of Medical ScienceUniversity of TorontoKITEToronto Rehabilitation InstituteUniversity Health NetworkTorontoONCanada

Reconstitution of complex multi-tissue organs is one of the most impressive feats of biology and is observed across regeneration-competent vertebrate species,including teleost fish(e.g.,zebrafish),urodeles(e.g.,axolotls and newts),and some *** ability within these species ranges from muscle(including cardiac),skeletal structures,to complex systems such as the brain,spinal cord and parts of the eye which are all capable of structural and functional repair following injury(Tanaka and Ferretti,2009).In stark contrast,re-establishment of multi-tissue structures is very rarely observed following embryogenesis in regeneration-incompetent *** of digit tips is the most dramatic example of mammalian regeneration,but pales in comparison to other species in the animal ***,a complete recapitulation of complex organs or structures in mammals will remain out of reach for a considerable time;however,an improved understanding of regenerat i ve mechanisms would likely enhance the development of novel regenerative medicine *** we focus on the diversity and commonalities of stem cells,which could underlie complex tissue regeneration.

关键词： organs rare potential

Global Landscape of Native Protein Complexes in Synechocystis *** 6803

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Genomics, Proteomics & Bioinformatics 2022年第4期20卷 715-727页

作者： Chen Xu Bing Wang Lin Yang Lucas Zhongming Hu Lanxing Yi Yaxuan Wang Shenglan Chen Andrew Emili Cuihong Wan School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology Central China Normal UniversityWuhan 430079China donnelly centre for cellular and biomolecular research University of TorontoTorontoON M5S 2E8Canada Departments of Biochemistry and Biology Boston UniversityBostonMA 02215USA

Synechocystis *** 6803(hereafter:Synechocystis)is a model organism for studying photosynthesis,energy metabolism,and environmental *** known as the first fully sequenced phototrophic organism,Synechocystis still has almost half of its proteome without functional *** this study,by using co-fractionation coupled with liquid chromatographytandem mass spectrometry(LC-MS/MS),we define 291 multi-protein complexes,encompassing24,092 protein±protein interactions(PPIs)among 2062 distinct gene *** information not only reveals the roles of photosynthesis in metabolism,cell motility,DNA repair,cell division,and other physiological processes,but also shows how protein functions vary from bacteria to higher plants due to changes in interaction *** also allows us to uncover the functions of hypothetical proteins,such as Sll0445,Sll0446,and Sll0447 involved in photosynthesis and cell motility,and Sll1334 involved in regulation of fatty acid *** we present the most extensive PPI data for Synechocystis so far,which provide critical insights into fundamental molecular mechanisms in cyanobacteria.

关键词： Protein–protein interaction Cyanobacterium Co-fractionation Photosynthesis Function prediction

Current Status and Prospect of Stent Placement for May-Thurner Syndrome

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Current Medical Science 2021年第6期41卷 1178-1186页

作者： Qin LIU Fang LIU Ping LÜ Hong-xiao WU Pin YE Yun YOU Zhong YAO Department of Vascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Nuclear Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China donnelly centre for cellular and biomolecular research University of TorontoTorontoOntario M5S 2E8Canada

Stent implantation has been proven to be safe and has become the first-line intervention for May-Thumer syndrome(MTS),with satisfactory mid-term patency rates and clinical *** research has demonstrated that catheter-directed thrombolysis is the preferred strategy when MTS is combined with deep vein thrombosis after self-expanding stent ***,the stent used for the venous system was developed based on the experience obtained in the treatment of arterial ***,relatively common corresponding complications may come along later,which include stent displacement,deformation,and *** measures such as adopting a stent with a larger diameter,improving stent flexibility,and increasing stent strength have been employed in order to prevent these *** ideal venous stent is presently being evaluated and will be introduced in detail in this review.

关键词： May-Thumer syndrome deep vein thrombosis post-thrombotic syndromes stent stent placement

Fibroadhesive scarring of grafted collagen scaffolds interferes with implant–host neural tissue integration and bridging in experimental spinal cord injury

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Regenerative Biomaterials 2019年第2期6卷 75-87页

作者： Haktan Altinova Sebastian Hammes Moniek Palm Jose Gerardo-Nava Pascal Achenbach Ronald Deumens Emmanuel Hermans Tobias Fuhrmann Arne Boecker Sabien Geraldine Antonia van Neerven Ahmet Bozkurt Joachim Weis Gary Anthony Brook Department of Neurosurgery RWTH Aachen University HospitalAachenGermany Institute of Neuropathology RWTH Aachen University HospitalAachenGermany Police Headquarters Berlin Medical CommissionBerlinGermany Institute of Neuroscience Universite Catholique de LouvainBrusselsBelgium donnelly centre for cellular&biomolecular research University of TorontoTorontoCanada Department of Hand- Plastic and Reconstructive SurgeryBurn Centre Trauma CentreBG Trauma Centre LudwigshafenUniversity of HeidelbergLudwigshafenGermany Department of Plastic Reconstructive and Hand SurgeryBurn CentreRWTH Aachen University HospitalAachenGermany Department of Plastic AestheticHand and Burn SurgeryHelios University Hospital WuppertalUniversity Witten/HerdeckeWuppertalGermany

Severe traumatic spinal cord injury(SCI)results in a devastating and permanent loss of function,and is currently an incurable *** is generally accepted that future intervention strategies will require combinational approaches,including bioengineered scaffolds,to support axon growth across tissue scarring and cystic ***,we demonstrated that implantation of a microporous type-I collagen scaffold into an experimental model of SCI was capable of supporting functional recovery in the absence of extensive implant–host neural tissue ***,we demonstrate the reactive host cellular responses that may be detrimental to neural tissue integration after implantation of collagen scaffolds into unilateral resection injuries of the adult rat spinal *** demonstrated scattered fibroblast-like cell infiltration throughout the scaffolds as well as the presence of variable layers of densely packed cells,the fine processes of which extended along the graft–host *** reactive astroglial or regenerating axonal profiles could be seen traversing this *** encapsulation-type behaviour around bioengineered scaffolds impedes the integration of host neural tissues and reduces the intended bridging role of the *** of the cellular and molecular mechanisms underpinning this behaviour will be pivotal in the future design of collagen-based bridging scaffolds intended for regenerative medicine.

关键词： spinal cord injury CNS-scarring type-I collagen scaffold astrogliosis fibrosis encapsulation

Principles of Nanoparticle Delivery to Solid Tumors

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Biomedical Engineering Frontiers 2023年第1期4卷 177-186页

作者： Warren C.W.Chan Institute of Biomedical Engineering University of TorontoTorontoON M5S 3G9Canada.Terrence Donnelly Center for Cellular&Biomolecular ResearchUniversity of TorontoTorontoON M53E1Canada.Department of ChemistryUniversity of TorontoTorontoON M5S 3H6Canada

The effective treatment of patients with cancer hinges on the delivery of therapeutics to a tumor *** provide an essential transport *** present 5 principles to consider when designing nanoparticles for cancer targeting:(a)Nanoparticles acquire biological identity in vivo,(b)organs compete for nanoparticles in circulation,(c)nanoparticles must enter solid tumors to target tumor components,(d)nanoparticles must navigate the tumor microenvironment for cellular or organelle targeting,and(e)size,shape,surface chemistry,and other physicochemical properties of nanoparticles influence their transport process to the *** review article describes these principles and their application for engineering nanoparticle delivery systems to carry therapeutics to tumors or other disease targets.

关键词： organs cancer carry

Biomaterials for immunomodulation in wound healing

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Regenerative Biomaterials 2024年第5期3卷 1-22页

作者： Ying Wang Katrina Vizely Chen Yu Li Karen Shen Amid Shakeri Ramak Khosravi James Ryan Smith Eugene Alfonzo II Alteza Yimu Zhao Milica Radisic Institute of Biomedical Engineering University of TorontoTorontoON M5S 3G9Canada Toronto General research Institute University Health NetworkTorontoON M5G 2C4 Canada Department of Chemical Engineering and Applied Chemistry University of TorontoTorontoON M5S 3E5Canada Division of Cardiovascular and Thoracic Surgery Department of SurgeryDuke University Medical CenterDurhamNC 27710USA Terrence donnelly centre for cellular&biomolecular research University of TorontoTorontoON M5S 3E1Canada

The substantial economic impact of non-healing wounds,scarring,and burns stemming from skin injuries is evident,resulting in a financial burden on both patients and the healthcare *** review paper provides an overview of the skin’s vital role in guarding against various environmental challenges as the body’s largest protective organ and associated developments in biomaterials for wound *** first introduce the composition of skin tissue and the intricate processes of wound healing,with special attention to the crucial role of immunomodulation in both acute and chronic *** highlights how the imbalance in the immune response,particularly in chronic wounds associated with underlying health conditions such as diabetes and immunosuppression,hinders normal healing ***,this review distinguishes between traditional wound-healing strategies that create an optimal microenvironment and recent peptide-based biomaterials that modulate cellular processes and immune responses to facilitate wound ***,we highlight the importance of considering the stages of wounds in the healing *** integrating advanced materials engineering with an in-depth understanding of wound biology,this approach holds promise for reshaping the field of wound management and ultimately offering improved outcomes for patients with acute and chronic wounds.

关键词： immunomodulation peptide-based biomaterials innate immunity angiogenesis for wound healing cellular response

A microfluidic platform for continuous monitoring of dopamine homeostasis in dopaminergic cells

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Microsystems & Nanoengineering 2019年第1期5卷 593-605页

作者： Yue Yu Richard P.Sde Campos Seolim Hong Dimitar L.Krastev Siddharth Sadanand Yen Leung Aaron R.Wheeler Institute for Biomaterials and Biomedical Engineering University of Toronto164 College StTorontoON M5s 3G9Canada donnelly centre for cellular and biomolecular research 160 College St.TorontoON M5S 3E1Canada Department of Chemistry University of Toronto80 St George St.TorontoON M5S 3H6Canada Department of Human Biology University of Toronto300 Huron StreetTorontoON M5S 3J6Canada

Homeostasis of dopamine,a classical neurotransmitter,is a key indicator of neuronal *** in the regulation of dopamine is implicated in a long list of neurological disorders,including addiction,depression,and *** existing methods used to evaluate dopamine homeostasis in vitro are inconvenient and do not allow for continuous non-destructive *** response to this challenge,we introduce an integrated microfluidic system that combines dopaminergic cell culture and differentiation with electroanalytical measurements of extracellular dopamine in real-time at any point during an *** used the system to examine the behavior of differentiated SH-SY5Y cells upon exposure to four dopamine transporter ant/agonists(cocaine,ketamine,epigallocatechin gallate,and amphetamine)and study their *** IC_(50)values of cocaine,ketamine,and epigallocatechin gallate were determined to be(average±standard deviation)3.7±1.1μM,51.4±17.9μM,and 2.6±0.8μM,***,we used the new system to study amphetamine-mediated dopamine release to probe the related phenomena of dopamine transporter-mediated reverse-transport and dopamine release from *** propose that this platform,which is the first platform to simultaneously evaluate uptake and release,could be useful to screen for drugs and other agents that target dopaminergic neurons and the function of the dopamine *** broadly,this platform should be adaptable for any application that could benefit from high-temporal resolution electroanalysis combined with multi-day cell culture using small numbers of cells.

关键词： dopamine homeostasis release

Glucose-6-phosphate dehydrogenase(G6PD) deficiency is associated with asymptomatic malaria in a rural community in Burkina Faso

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Asian Pacific Journal of Tropical Biomedicine 2014年第8期4卷 655-658页

作者： Abdoul Karim Ouattara Cyrille Bisseye Bapio Valery Jean Télesphore Elvira Bazie Birama Diarra Tegwindé Rebeca Compaore Florencia Djigma Virginio Pietra Remy Moret Jacques Simpore centre for biomolecular research Pietro Annigoni(CERBA) LABIOGENE UFR/SVT University of Ouagadougou BP 364 OuagadougouBurkina Faso Laboratory of Molecular and cellular Biology(LABMC) University of Science and Technology of Masuku(USTM)BP 943 FrancerilleGabon

Objective:To investigate 4 combinations of mutations responsible for glucose-6—phosphate dehydrogenase(G6PD) deficiency in a rural community of Burkina Faso,a malaria endemic ***:Two hundred individuals in a rural community were genotyped for the mutations A376 G.G202A,A542 T,G680T and T968 C using TaqMan single nucleotide polymorphism assays and polymerase chain reaction followed by restriction fragment length ***:The prevalence of the G6 PD deficiency was 9.5%,in the study *** was significantly higher in men compared to women(14.23%vs 6.0%,P=0.049).The 202A/376 G G6PD Awas the only deficient variant *** falciparum asymptomatic parasitemia was significantly higher among the C6PD-non—deficient persons compared to the G6PD-deficient(P<0.001).The asymptomatic parasitemia was also significantly higher among G(SPI) nondeficient compared to C6PD—heterozygous females(P<0.001).Conclusions:This study showed that the G6 PD A- variant associated with protection against asymptomatic malaria in Burkina Faso is probably the most common deficient variant.

关键词： Polymerase chain reaction Mutations Glucose-6-phosphate dehydrogenase deficiency Asymptomatic malaria Burkina Faso

The influence of nanotopography on cell behaviour through interactions with the extracellular matrix-A review

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Bioactive Materials 2022年第9期7卷 145-159页

作者： Jiajun Luo Matthew Walker Yinbo Xiao Hannah donnelly Matthew J.Dalby Manuel Salmeron-Sanchez centre for the cellular Microenvironment University of GlasgowGlasgowG128LTUK

Nanotopography presents an effective physical approach for biomaterial cell manipulation mediated through material-extracellular matrix *** extracellular matrix that exists in the cellular microenvironment is crucial for guiding cell behaviours,such as determination of integrin ligation and interaction with growth *** interactions with the extracellular matrix regulate downstream mechanotransductive pathways,such as rearrangements in the cytoskeleton and activation of signal *** adsorption onto nanotopography strongly influences the conformation and distribution density of extracellular matrix and,therefore,subsequent cell *** this review,we first discuss the interactive mechanisms of protein physical adsorption on ***,we summarise advances in creating nanotopographical features to instruct desired cell ***,we focus on the cellular mechanotransductive pathways initiated by *** review provides an overview of the current state-of-the-art designs of nanotopography aiming to provide better biomedical materials for the future.

关键词： Nanotopography Cell-material interaction Protein adsorption Biomaterials