检索结果-南通市图书馆

Effect of herpesvirus infection on pancreatic duct cell secretion

World Journal of Gastroenterology 2005年第38期11卷 5997-6002页

作者： Péter Hegyi Balázs rdg Zoltán Rakonczai Jr Tamás Takács János Lonovics Annamária Szabolcs Réka Sári András Tóth Julius G Papp András Varró Mária K Kovács Mike A Gray Barry E Argent Zsolt Boldogki First Department of medicine Department of Biology division of Cardiovascular Pharmacology School of Cell and molecular Biosciences

AIM： To examine the effect of acute infection caused by herpesvirus （pseudorabies virus, PRV） on pancreatic ductal secretion. METHODS： The virulent Ba-DupGreen （BDG） and non- virulent Ka-RREp01acgfp （KEG） genetically modified strains of PRV were used in this study and both of them contain the gene for green fluorescent protein （GFP）. Small intra/ interlobular ducts were infected with BDG virus （10^7 PFU/mL for 6 h） or with KEG virus （10^10 PFU/mL for 6 h）, while non-infected ducts were incubated only with the culture media. The ducts were then cultured for a further 18 h. The rate of HCO3 secretion [base efflux -λ（B）] was determined from the buffering capacity of the cells and the initial rate of intracellular acidification （1） after sudden blockage of basolateral base loaders with dihydro-4,4,- diisothiocyanatostilbene-2,2,-disulfonic acid （500 μmol/L） and amiloride （200 μmol/L）, and （2） after alkali loading the ducts by exposure to NH4Cl. All the experiments were performed in HCO3-buffered Ringer solution at 37 ℃ （n = 5 ducts for each experimental condition）. Viral structural proteins were visualized by immunohistochemistry. Virallyencoded GFP and immunofluorescence signals were recorded by a confocal laser scanning microscope.RESULTS： The BDG virus infected the majority of accessible cells of the duct as judged by the appearance of GFP and viral anUgens in the ductal cells. KEG virus caused a similarly high efficiency of infection. After blockage of basolateral base loaders, BDG infection significantly elevated -λB-） 24 h after the infection, compared to the non-infected group. However, KEG infection did not modify -λB-）. After alkali loading the ducts, -λB-） was significantly elevated in the BDG group compared to the control group 24 h after the infection. As we found with the inhibitor stop method, no change was observed in the group KEG compared to the non-infected group. CONCLUSION： Incubation with the BDG or KEG strains of PRV results in an effective i

关键词： Pancreas Hypersecretion Ductal cells Infection Pseudorabies virus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Metabolic effects of intestinal absorption and enterohepatic cycling of bile acids

引用

Acta Pharmaceutica Sinica B 2015年第2期5卷 129-134页

作者： Courtney B.Ferrebee Paul A.Dawson Department of Pediatrics Division of Gastroenterology Hepatology and Nutrition Emory University molecular medicine Graduate Program Wake Forest School of Medicine

The classical functions of bile acids include acting as detergents to facilitate the digestion and absorption of nutrients in the gut. In addition, bile acids also act as signaling molecules to regulate glucose homeostasis, lipid metabolism and energy expenditure. The signaling potential of bile acids in compartments such as the systemic circulation is regulated in part by an efficient enterohepatic circulation that functions to conserve and channel the pool of bile acids within the intestinal and hepatobiliary compartments. Changes in hepatobiliary and intestinal bile acid transport can alter the composition, size,and distribution of the bile acid pool. These alterations in turn can have significant effects on bile acid signaling and their downstream metabolic targets. This review discusses recent advances in our understanding of the inter-relationship between the enterohepatic cycling of bile acids and the metabolic consequences of signaling via bile acid-activated receptors, such as farnesoid X nuclear receptor(FXR)and the G-protein-coupled bile acid receptor(TGR5).

关键词： Bile acids Liver Intestine Transporters Lipid metabolism Energy homeostasis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Novel Y-chromosome Short Tandem Repeat Variants Detected Through the Use of Massively Parallel Sequencing

引用

genomics, Proteomics & Bioinformatics 2015年第4期13卷 250-257页

作者： David H.Warshauer Jennifer D.Churchill Nicole Novroski Jonathan L.King Bruce Budowle Institute of Applied Genetics Department of Molecular and Medical Genetics University of North Texas Health Science Center Center of Excellence in genomic medicine Research (CEGMR) King Abdulaziz University

Massively parallel sequencing （MPS） technology is capable of determining the sizes of short tandem repeat （STR） alleles as well as their individual nueleotide sequences. Thus, single nucleotide polymorphisms （SNPs） within the repeat regions of STRs and variations in the pattern of repeat units in a given repeat motif can be used to differentiate alleles of the same length. In this study, MPS was used to sequence 28 forensically-relevant Y-chromosome STRs in a set of 41 DNA samples from the 3 major U.S. population groups （African Americans, Caucasians, and Hispanics）. The resulting sequence data, which were analyzed with STRait Razor v2.0, revealed 37 unique allele sequence variants that have not been previously reported. Of these, 19 sequences were variations of documented sequences resulting from the presence of intra-repeat SNPs or alternative repeat unit patterns. Despite a limited sampling, two of the most frequently-observed variants were found only in African American samples. The remaining 18 variants represented allele sequences for which there were no published data with which to compare. These findings illustrate the great potential of MPS with regard to increasing the resolving power of STR typing and emphasize the need for sample population characterization of STR alleles.

关键词： Y-STR Sequence polymorphism Allele variants Massively parallel sequencing Nextera STRait Razor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The role of BRCA1 in DNA damage response

引用

Protein & Cell 2010年第2期1卷 117-123页

作者： Jiaxue Wu Lin-Yu Lu Xiaochun Yu division of molecular medicine and Genetics Department of Internal MedicineUniversity of Michigan Medical SchoolAnn ArborMI 48109USA

BRCA1 is a well-established tumor suppressor gene,which is frequently mutated in familial breast and ovarian *** gene product of BRCA1 functions in a number of cellular pathways that maintain genomic stability,including DNA damage-induced cell cycle checkpoint activation,DNA damage repair,protein ubiquitination,chromatin remodeling,as well as transcriptional regulation and *** this review,we discuss recent advances regarding our understanding of the role of BRCA1 in tumor suppression and DNA damage response,including DNA damage-induced cell cycle checkpoint activation and DNA damage repair.

关键词： BRCA1 DNA damage response tumorigenesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification and expression profiling of Vigna mungo microRNAs from leaf small RNA transcriptome by deep sequencing

引用

Journal of Integrative Plant Biology 2014年第1期56卷 15-23页

作者： Sujay Paul Anirban Kundu Amita Pal division of Plant Biology Bose Institute Center for genomic Sciences National Autonomous University of Mexico

MicroRNAs （miRNAs） represent a class of small non-coding RNA molecules that play a crucial role in post- transcriptional gene regulation. Several conserved and species-specific miRNAs have been characterized to date, predominantly from the plant species whose genome is well characterized. However, information on the variability of these regulatory RNAs in economically important but genetically less characterized crop species are limited. Vigna mungo is an important grain legume, which is grown primarily for its protein-rich edible seeds, miRNAs from this species have not been identified to date due to lack of genome sequence information. To identify miRNAs from V. mungo, a small RNA library was constructed from young leaves. High- throughput IIlumina sequencing technology and bioinformat- ic analysis of the small RNA reads led to the identification of 66 miRNA loci represented by 45 conserved miRNAs belonging to 19 families and eight non-conserved miRNAs belonging to seven families. Besides, 13 novel miRNA candidates in V. mungo were also identified. Expressior patterns of selected conserved, non-conserved, and nove miRNA candidates have been demonstrated in leaf, stem, ant root tissues by quantitative polymerase chain reaction, ant potential target genes were predicted for most of the conserved miRNAs. This information offers genomic resour ces for better understanding of miRNA mediated post transcriptional gene regulation.

关键词： Illumina microRNA next generation sequencing quantitative polymerase chain reaction target transcript

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Practical fecal calprotectin cut-off value for Japanese patients with ulcerative colitis

引用

World Journal of Gastroenterology 2018年第38期24卷 4384-4392页

作者： Jun Urushikubo Shunichi Yanai Shotaro Nakamura Keisuke Kawasaki Risaburo Akasaka Kunihiko Sato Yosuke Toya Kensuke Asakura Takahiro Gonai Tamotsu Sugai Takayuki Matsumoto division of Gastroenterology Department of Internal Medicine School of Medicine Iwate Medical University division of molecular Diagnostic Pathology Department of Pathology School of Medicine Iwate Medical University

AIM To determine appropriate fecal calprotectin cut-off values for the prediction of endoscopic and histologic remission in Japanese patients with ulcerative colitis(UC). METHODS We performed a cross-sectional observational study of 131 Japanese patients with UC and measured fecal calprotectin levels by fluorescence enzyme immunoassay. The clinical activity of UC was assessed with the partial Mayo score(PMS). Relapse was defined as increase of PMS by 2 points or more in stool frequency or rectal bleeding subscore. The endoscopic and histologic activities of UC were evaluated in 50 patients within a 2-mo period from fecal sampling. Endoscopic activity was determined by Mayo endoscopic subscore, Rachmilewitz endoscopic index, and ulcerative colitis endoscopic index of severity. The histologic grade of inflammation was evaluated with biopsy specimens obtained from the endoscopically most severely inflamed site, according to the scheme by Matts grade and Riley's *** Fecal calprotectin levels varied from 1-20783 μg/g. There was a significant correlation between the partial Mayo score and fecal calprotectin levels(r = 0.548, P < 0.001). In 50 patients who underwent colonoscopy with biopsy, levels were significantly correlated with the Mayo endoscopic subscore(r = 0.574, P < 0.001), Rachmilewitz endoscopic index(r = 0.628, P < 0.001), ulcerative colitis endoscopic index of severity(r = 0.613, P < 0.001), Riley's histologic score(r = 0.400, P = 0.006), and Matts grade(r = 0.586, P < 0.001). Receiver-operating characteristic analyses identified the best cut-off value for the prediction of endoscopic remission as 288 μg/g, with an area under the curve of 0.777 or 0.823, while that for histologic remission was 123 or 125 μg/g, with an AUC of 0.881 or 0918, respectively. Of the 131 study patients, 88 patients in clinical remission were followed up 6 mo. During the follow-up period, 19 patients relapsed. The best fecal calprotectin cut-off value for predicting relapse

关键词： Ulcerative colitis Remission Mucosal healing Colonoscopy Histology Biomarker Feces Calprotectin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pancreatic acinar cell carcinoma: A review on molecular profiling of patient tumors

引用

World Journal of Gastroenterology 2017年第45期23卷 7945-7951页

作者： Ahmad Al-Hader Rami N Al-Rohil Haiyong Han Daniel Von Hoff division of Hematology/Oncology Indiana University School of Medicine Department of Pathology Vanderbilt University School of Medicine molecular medicine division Translational Genomics Research Institute (TGen)

Pancreatic carcinomas with acinar differentiation are rare,accounting for 1%-2% of adult pancreatic tumors; they include pancreatic acinar cell carcinoma(PACC),pancreatoblastoma,and carcinomas of mixed differentiation. Patients with PACC have a prognosis better than pancreatic ductal adenocarcinomas but worse than pancreatic neuroendocrine tumors. Reports of overall survival range from 18 to 47 mo. A literature review on PACCs included comprehensive genomic profiling and whole exome sequencing on a series of more than 70 patients as well as other diagnostic studies including immunohistochemistry. Surgical resection of PACC is the preferred treatment for localized and resectable tumors. The efficacy of adjuvant treatment is unclear. Metastatic PACCs are generally not curable and treated with systemic chemotherapy. They are moderately responsive to chemotherapy with different regimens showing various degrees of response in case reports/series. Most of these regimens were developed to treat patients with pancreatic ductal adenocarcinomas or colorectal adenocarcinomas. Review of PACC's molecular profiling showed a number of gene alterations such as: SMAD4,BRAF,BRCA2,TP53,RB1,MEN1,JAK-1,BRCA-1,BRCA-2,and DNA mismatch repair abnormalities. PACCs had multiple somatic mutations with some targetable with available drugs. Therefore,molecular profiling of PACC should be an option for patients with refractory PACC.

关键词： Pancreatic acinar cell carcinoma molecular profiling Targeted therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regulation of ferroptosis in cancer cells by YAP/TAZ and Hippo pathways:The therapeutic implications

引用

Genes & Diseases 2021年第3期8卷 241-249页

作者： Tianai Sun Jen-Tsan Chi Department of molecular Genetics and Microbiology Duke University School of MedicineDurhamNC27710USA Center for genomic and Computational Biology Duke University School of MedicineDurhamNC27710USA

Ferroptosis is a novel form of iron-dependent cell death characterized by lipid *** the importance and disease relevance of ferroptosis is gaining recognition,much remains unknown about various genetic and non-genetic determinants of *** signaling pathway is an evolutionarily conserved pathway that responds to various envi-ronmental cues and controls organ size,cell proliferation,death,and self-renewal *** cancer biology,Hippo pathway is a potent tumor suppressing mechanism and its dysregulation contributes to apoptosis evasion,cancer development,metastasis,and treatment *** dysregulation leads to aberrant activation of YAP and TAZ,the two major transcription co-activators of TEADs,that induce the expression of genes triggering tumor-promoting pheno-types,including enhanced cell proliferation,self-renewal and apoptosis *** Hippo pathway is regulated by the cell-cell contact and cellular density/***,fer-roptosis has also been found being regulated by the cellular contact and *** YAP/TAZ activation under low density,while confers apoptosis resistance,renders cancer cells sensitivity to *** findings establish YAP/TAZ and Hippo pathways as novel deter-minants of ***,inducing ferroptosis may have therapeutic potential for YAP/TAZ-activated chemo-resistant and metastatic tumor ***,various YAP/TAZ-targeting treatments under clinical development may confer ferroptosis resistance,limiting the therapeutic efficacy.

关键词： Apoptosis Ferroptosis Hippo pathway Transcriptional coactivator with PDZ-binding motif(TAZ) Yes-associated protein 1(YAP1)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Thai family with hereditary pancreatitis and increased cancer risk due to a mutation in PRSS1 gene

引用

World Journal of Gastroenterology 2005年第11期11卷 1634-1638页

作者： Theeraphong Pho-Iam Wanna Thongnoppakhun Pa-Thai Yenchitsomanus Chanin Limwongse division of molecular Genetics division of Medical Genetics

AIM: To investigate mutation of serine protease 1-cationic trypsinogen (CT, PRSS1) gene in members of a Thai family with hereditary pancreatitis and pancreatic cancer. METHODS: Polymerase chain reaction and direct sequencing were performed to analyze the PRSS1 gene in two members of the family affected by pancreatitis. Allele specific amplification (ASA) method was then developed to detect the mutation of the PRSS1 gene in all available members of the family and normal control subjects. RESULTS: A cytosine (C) to thymine (T) mutation at position 2441 (g.2441C>T) of the PRSS1 gene, which results in a substitution of arginine by cysteine at position 116 (R116C) of CT, was identified by direct sequencing in both clinically affected members of the family but was not found in the unaffected member. This mutation, which might be arising from deamination of methylated cytosine in CpG dinucleotide of codon 116 (CGT>TGT), was also detected by the ASA method in the two affected members and a proband's brother but was not observed in unaffected members and 54 normal control subjects. CONCLUSION: Autosomal dominant pancreatitis with increased cancer risk in the studied Thai family is most likely due to missense (R116C) mutation in the PRSS1 gene.

关键词： PRSS1 Hereditary pancreatitis Pancreatic cancer Thai R116C

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CXCL12/SDF-1α Activates NF-κB and Promotes Oral Cancer Invasion through the Carma3/Bcl10/Malt1 Complex

引用

International Journal of Oral Science 2009年第3期1卷 105-118页

作者： Aasia O. Rehman Cun-yu Wang Laboratory of molecular Signaling Division of Oral Biology and Medicine UCLA School of Dentistry Los Angeles USA

Aim To determine how SDF-1α/CXCR4 activates nuclear factor-kappa B （NF-κB） and promotes oral squamous cell carcinoma （OSCC） *** A lentivirus-based knockdown approach was utilized to deplete gene expression. NF-κB activation was evaluated by Western blot analysis and electrophoretic mobility shift （EMSA）. Results We show that the activation of NF-κB by CXCR4 occurs through the Carma3/Bcl10/Maltl （CBM） complex in OSCC. We found that loss of components of the CBM complex in HNSCC can inhibit SDF-1α induced phosphorylation and degradation of IκBα, while TNFα induced IKK activation remains unchanged. Further, we identified a role for novel and atypical, but not classical, PKCs in activating IKK through CXCR4. Importantly, inhibition of the CBM complex leads to a significant decrease in SDF-1α mediated invasion of OSCC. Conclusion The CBM complex plays a critical role in CXCR4-induced NF-κB activation in OSCC. Targeting molecular components of the NF-κB signaling pathway may provide an important therapeutic opportunity in controlling the progression and metastasis of OSCC mediated by SDF-1α.

关键词： CXCR4 NF-κB head and neck cancer invasion signal transduction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：