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Accelerated mineralization on nanofibers via non-thermal atmospheric plasma assisted glutamic acid templated peptide conjugation

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Regenerative biomaterials 2019年第4期6卷 231-240页

作者： Günnur Onak Ozan Karaman tissue engineering and Regenerative Medicine Laboratory Department of Biomedical Engineering_Izmir Katip C¸elebi University_Izmir 35620Turkey Bonegraft biomaterials Co. Ege University TechnopolisBornova_Izmir 35100Turkey

Surface modification by non-thermal atmospheric plasma(NTAP)treatment can produce significantly higher carboxylic groups on the nanofibers(NF)surface,which potentially can increase biomineralization of NF via promoting glutamic acid(GLU)templated peptide ***,electrospun poly(lactide-co-glycolide)(PLGA)scaffolds were treated with NTAP and conjugated with GLU peptide followed by incubation in simulated body fluids for *** effect of NTAP treatment and GLU peptide conjugation on mineralization,surface wettability and roughness were *** results showed that NTAP treatment significantly increased GLU peptide conjugation which consequently enhanced mineralization and mechanical properties of NTAP treated and peptide conjugated NF(GLU-pNF)compared to neat PLGA NF,NTAP treated NF(pNF)and GLU peptide conjugated NF(GLU-NF).The effect of surface modification on human bone marrow derived mesenchymal stem cells adhesion,proliferation and morphology was evaluated by cell proliferation assay and fluorescent *** demonstrated that cellular adhesion and proliferation were significantly higher on GLU-pNF compared to NF,pNF and *** summary,NTAP treatment could be a promising modification technique to induce biomimetic peptide conjugation and biomineralization for bone tissue engineering applications.

关键词： nanofibers biomineralization non-thermal atmospheric plasma bone tissue engineering

An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs

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Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： Seol-Ha Jeong Jarno Hiemstra Patrick V.Blokzijl Rebeca Damian-Ferrara Danilo Martins dos Santos Jéssica H.L.da Fonseca Min-Ho Kang Jihyun Kim Dilara Yilmaz-Aykut Mei L.L.Cham-Pérez Jeroen Leijten Su Ryon Shin division of engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA Leijten Lab Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnology National Laboratory for Agriculture(LNNA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical-Chemical engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotechnology The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

Ensuring a sufficient oxygen supply is pivotal for the success of bioprinting applications since it fosters tissue integration and natural *** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen levels in imprinted constructs to support the survival of targeted *** oxygen-releasing biomaterials,such as oxygen-generating microparticles(OMPs),have shown promise for enhancing the oxygen supply of microenvironments in injured tissues,whether this approach is scalable for large tissues and whether tissue-specific bioinks with varying OMP concentrations remain printable remain *** study addresses this critical gap by introducing an innovative class of engineered oxygenated bioinks that combine colloidal-based microgels with *** report that incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic polymeric microparticles enables precise modulation of oxygen release while controlling hydrogen peroxide ***,the fabrication of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-phase *** study thoroughly evaluates the fundamental characteristics of the resulting bioink,including its rheological behaviors,printability,shape fidelity,mechanical properties,and oxygen release ***,this study demonstrates the macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal *** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential for new *** findings presented here advance the bioprinting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated *** work thereby offers a promising avenue for the development of functional tissues with e

关键词： 3D bioprinting Bioink Colloidal gels Extrusion printing Oxygen-generating microparticle

Magnesium implant degradation provides immunomodulatory and proangiogenic effects and attenuates peri-implant fibrosis in soft tissues

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Bioactive Materials 2023年第8期26卷 353-369页

作者： Heithem Ben Amara Diana C.Martinez Furqan A.Shah Anna Johansson Loo Lena Emanuelsson Birgitta Norlindh Regine Willumeit-Römer Tomasz Plocinski Wojciech Swieszkowski Anders Palmquist Omar Omar Peter Thomsen Department of biomaterials Institute of Clinical SciencesSahlgrenska AcademyUniversity of GothenburgGothenburgSweden biomaterials Group Materials Design DivisionFaculty of Materials Science and EngineeringWarsaw University of TechnologyWarsawPoland Institute of Metallic biomaterials Helmholtz-Zentrum HereonGeesthachtGermany

Implants made of magnesium(Mg)are increasingly employed in patients to achieve osteosynthesis while degrading in *** Mg implants and Mg^(2+)have been suggested to possess anti-inflammatory properties,the clinically observed soft tissue inflammation around Mg implants is ***,using a rat soft tissue model and a 1-28 d observation period,we determined the temporo-spatial cell distribution and behavior in relation to sequential changes of pure Mg implant surface properties and Mg^(2+)*** to nondegradable titanium(Ti)implants,Mg degradation exacerbated initial *** of Mg degradation products at the tissue-implant interface,culminating at 3 d,actively initiated chemotaxis and upregulated mRNA and protein immunomodulatory markers,particularly inducible nitric oxide synthase and toll-like receptor-4 up to 6 d,yet without a cytotoxic *** vascularization was demonstrated morphologically,preceded by high expression of vascular endothelial growth *** transition to appropriate tissue repair coincided with implant surface enrichment of Ca and P and reduced peri-implant Mg^(2+)*** implants revealed a thinner fibrous encapsulation compared with *** detailed understanding of the relationship between Mg material properties and the spatial and time-resolved cellular processes provides a basis for the interpretation of clinical observations and future tailoring of Mg implants.

关键词： Magnesium Biodegradable implants Inflammation Foreign-body reaction Gene expression Neovascularization

Alginate/gelatin/boron-doped hydroxyapatite-coated Ti implants:in vitro and in vivo evaluation of osseointegration

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Bio-Design and Manufacturing 2023年第3期6卷 217-242页

作者： Ahmet E.Pazarçeviren Zafer Evis Tayfun Dikmen Korhan Altunbas Mustafa V.Yaprakçı Dilek Keskin Aysen Tezcaner Department of engineering Sciences Middle East Technical UniversityAnkaraTurkey Department of Histology and Embryology Afyon Kocatepe UniversityAfyonTurkey Department of Surgery Afyon Kocatepe UniversityAfyonTurkey Center of Excellence in biomaterials and tissue engineering AnkaraTurkey

In this study,boron-doped hydroxyapatite(BHT)-loaded alginate/gelatin-based(A/G)hydrogel coating on Ti was fabricated to support bone integration through triggering osteoinduction,vascularization and ***,highly reproducible,cheap and time-effective BHT was produced,which significantly promoted higher osteogenic and angiogenic maturation,while a mild innate immune response was *** immense potential of BHT was evidenced by the production of a gap-filling A/G/BHT interphase on Ti implants to mimic the osseous extracellular matrix to achieve functional bridging and exert control over the course of innate immune *** initially aminosilanized the implant surface using 3-aminopropyl triethoxysilane,and then coated it with 0.25%w/v alginate with 20 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide to allowthe A/G/BHT pre-gel to disperse evenly and covalently attach on the *** pre-gel was added with 0.2 M NaCl to homogeneously blend BHT in the structure without inducing ionic ***,the coated implants were freeze-dried and *** coated layer demonstrated high cohesive and adhesive strength,and 8-month-long shelf-life at room temperature and normal *** A/G/BHT was able to coat an irregularly shaped Ti *** and endothelial cells thrived on the A/G/BHT,and it demonstrated greatly improved osteogenic and angiogenic ***,A/G/BHT maintained macrophage viability and generated an acute increase in immune response that could be resolved ***,A/G/BHT was shown to induce the robust integration of implant in a rabbit femur osteochondral model within ***,we concluded that A/G/BHT coatings could serve as amultifunctional reservoir,promoting the strong and rapid osseointegration of metallic implants.

关键词： Boron Alginate/gelatin Implant coating Titanium Osteochondral model

Aldehyde-Sodium Alginate and Amino-Gelatin Preparation as Soft tissue Adhesive

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Journal of Donghua University(English Edition) 2014年第4期31卷 503-506页

作者：袁柳耿晓华李家俊孙彬彬莫秀梅 State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua University biomaterials and tissue engineering Laboratory College of ChemistryChemical Engineering and BiotechnologyDonghua University

Sodium alginate and gelatin are both remarkable natural biomaterials; they all have been extensively applied in tissue engineering and other relative fields,due to their low price and good biocompatibility. In this paper,we oxidized sodium alginate with sodium periodate to convert 1,2-hydroxyl groups into aldehyde groups to get aldehyde-sodium alginate( A-SA). Gelatin was modified with ethylenediamine( ED) in the presence of water-soluble1-ethyl-3( 3-dimethylaminopropyl) carbodiimide( EDC) to introduce additional amino groups to get amino-gelatin. Upon mixing the A-SA and amino-gelatin aqueous solutions together,a gel rapidly formed based on the Schiff's base reaction between the aldehyde groups in A-SA and the amino groups in *** transform infrared spectroscopy( FTIR) analysis confirmed the characteristic peak of Schiff's base group in the hydrogel. The gelation time measure has confirmed the gelation time is dependent on the aldehyde group content in A-SA and amino group content in amino-gelatin. The fasted hydrogel formation takes place within 30 s. The entire test suggested that this gel could be a promising candidate as soft tissue adhesive.

关键词： oxidized sodium alginate amino-gelatin adhesive agent

Supercritical fluid-assisted controllable fabrication of open and highly interconnected porous scaffolds for bone tissue engineering

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Science China(Life Sciences) 2019年第12期62卷 1670-1682页

作者： Hanxiao Tang Ranjith Kumar Kankala Shibin Wang Aizheng Chen College of Chemical engineering Huaqiao UniversityXiamen 361021China Institute of biomaterials and tissue engineering Huaqiao UniversityXiamen 361021China Fujian Provincial Key Laboratory of Biochemical Technology(Huaqiao University) Xiamen 361021China

Recently tremendous progress has been evidenced by the advancements in developing innovative three-dimensional(3 D)scaffolds using various techniques for addressing the autogenous grafting of bone. In this work, we demonstrated the fabrication of porous polycaprolactone(PCL) scaffolds for osteogenic differentiation based on supercritical fluid-assisted hybrid processes of phase inversion and foaming. This eco-friendly process resulted in the highly porous biomimetic scaffolds with open and interconnected architectures. Initially, a 2^3 factorial experiment was designed for investigating the relative significance of various processing parameters and achieving better control over the porosity as well as the compressive mechanical properties of the scaffold. Then, single factor experiment was carried out to understand the effects of various processing parameters on the morphology of scaffolds. On the other hand, we encapsulated a growth factor, i.e., bone morphogenic protein-2(BMP-2), as a model protein in these porous scaffolds for evaluating their osteogenic differentiation. In vitro investigations of growth factor loaded PCL scaffolds using bone marrow stromal cells(BMSCs) have shown that these growth factor-encumbered scaffolds were capable of differentiating the cells over the control experiments. Furthermore, the osteogenic differentiation was confirmed by measuring the cell proliferation, and alkaline phosphatase(ALP) activity, which were significantly higher demonstrating the active bone growth. Together, these results have suggested that the fabrication of growth factor-loaded porous scaffolds prepared by the eco-friendly hybrid processing efficiently promoted the osteogenic differentiation and may have a significant potential in bone tissue engineering.

关键词： supercritical foaming polycaprolactone bone tissue engineering osteogenic differentiation bone morphogenic protein-2

Probing the mechanosensitivity in cell adhesion and migration: Experiments and modeling

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Acta Mechanica Sinica 2013年第4期29卷 469-484页

作者： Bao-Hua Ji Bo Huo Biomechanics and biomaterials Laboratory School of Aerospace Engineering Beijing Institute of Technology

Cell adhesion and migration are basic physiolog- ical processes in living organisms. Cells can actively probe their mechanical micro-environment and respond to the ex- ternal stimuli through cell adhesion. Cells need to move to the targeting place to perform function via cell migration. For adherent cells, cell migration is mediated by cell-matrix adhesion and cell-cell adhesion. Experimental approaches, especially at early stage of investigation, are indispensable to studies of cell mechanics when even qualitative behaviors of cell as well as fundamental factors in cell behaviors are unclear. Currently, there is increasingly accumulation of ex- perimental data of measurement, thus a quantitative formula- tion of cell behaviors and the relationship among these fun- damental factors are highly needed. This quantitative under- standing should be crucial to tissue engineering and biomed- ical engineering when people want to accurately regulate or control cell behaviors from single cell level to tissue level. In this review, we will elaborate recent advances in the ex- perimental and theoretical studies on cell adhesion and mi- gration, with particular focuses laid on recent advances in experimental techniques and theoretical modeling, through which challenging problems in the cell mechanics are sug- gested.

关键词： Cell mechanics ~ Cell adhesion. Cell migration.Modeling ~ Mechanobiology

Photosynthetic co-culture system of algae and human umbilical vein endothelial cells:The effect on alleviating hypoxia and hypoxia/reoxygenation injury

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Biosurface and Biotribology 2024年第2期10卷 76-88页

作者： Donghu Lin Yuanyuan Chen Xinyu Tao Xin Che Shiyu Li Shiyu Cheng Shuxin Qu Institute of Biomedical engineering College of MedicineSouthwest Jiaotong UniversityChengduSichuanChina Key Laboratory of Advanced Technologies of Materials Ministry of Education School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengduSichuanChina School of Life Science and engineering Southwest Jiaotong UniversityChengduSichuanChina

It is a developed photosynthetic co-culture system to alleviate the hypoxia and hypoxia/reoxygenation(H/R)-injured human umbilical vein endothelial cells(HUVECs).The algae,Chlorella vulgaris,were encapsulated to slow their growth while not affecting the photosynthetic oxygen-producing capacity by Layer-by-layer(LbL)using gelatin and sodium alginate as the positive and negative charges materials,***,the photosynthetic co-culture system of HUVECs and self-oxygenating alginate hydrogel(Algae-gel)was constructed in which the optimal ratios between algae and HUVECs were 5:1 and 20:1 for a 2D or 3D co-cultured manner,*** indicated that the 3D cocultured manner of HUVECs needed more O_(2) by the production of algae than it did in a 2D co-cultured *** co-cultured Algae-gel could alleviate hypoxia and the oxidative stress injury of hypoxia and hypoxia/reoxygenation(H/R)-treated HUVECs in the proliferation,intracellular ROS and cellular migratory *** addition,the Algaegel could downregulate the expression of hypoxia-inducible factors 1α(HIF-1α)and vascular endothelial growth factor(VEGF)of hypoxia and H/R-injured HUVECs due to the improvement of hypoxia and H/R *** photosynthetic co-culture system could offer a promising approach for repairing hypoxia and H/R-injured cells or tissue by providing safe and stable O_(2).

关键词： biomaterials biomedical application bionic design regenerative medicine self-assembly tissue engineering

Restoring cellular calcium homeostasis to rescue ER stress by 1,2-bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid acetoxymethyl ester-loaded lipid-mPLGA hybrid-nanoparticles for acute kidney injury therapy

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Chinese Chemical Letters 2024年第3期35卷 337-345页

作者： Jingwen Zhang Jiahui Yan Yanan Wang Hong Liu Xueping Sun Yuchao Gu Liangmin Yu Changcheng Li Jun Wu Zhiyu He Frontiers Science Center for Deep Ocean Multispheres and Earth System and Key Laboratory of Marine Chemistry Theory and TechnologyMinistry of EducationOcean University of ChinaQingdao 266100China College of Chemistry and Chemical engineering Ocean University of ChinaQingdao 266100China School of Medicine and Pharmacy Ocean University of ChinaQingdao 266003China Sanya Oceanographic Institution Ocean University of ChinaQingdao 572024China Bioscience and Biomedical engineering Thrust The Hong Kong University of Science and Technology(Guangzhou)NanshaGuangzhou 511400China division of Life Science The Hong Kong University of Science and TechnologyHong Kong 999077China

Early pathogenesis of ischemia-reperfusion(I/R)-induced acute kidney injury(AKI)is dominated by intracellular calcium overload,which induces oxidative stress,intracellular energy metabolism disorder,inflammatory activation,and a series of pathologic cascaded reactions that are closely intertwined with self-amplifying and interactive feedback loops,ultimately resulting in cell damage and kidney ***,most nanomedicines originate from the perspective of antioxidant stress,which can only quench existing reactive oxide species(ROS)but cannot prevent the continuous production of ROS,resulting in insufficient *** a safe and promising drug,BAPTA-AM is hydrolyzed into BAPTA by intracellular esterase upon entering cells,which can rapidly chelate with overloaded Ca^(2+),restoring intracellular calcium homeostasis,thus inhibiting ROS regeneration at the ***,we designed a KTP-targeting peptide-modified yolk-shell structure of liposome–poly(ethylene glycol)methyl ether-block-poly(L-lactide-co-glycolic)(mPLGA)hybrid nanoparticles(tissue,thereby inhibiting the expression of renal tubular marker kidney injury mole

关键词： BAPTA-AM Calcium overload Acid-responsive AKI ER stress