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Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly

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Journal of Molecular Cell biology 2020年第8期12卷 654-665页

作者： Xu Liu Leilei Xu Junying Li Phil Y.Yao Wanjuan Wang Hazrat Ismail Haowei Wang Bryce Liao Zhihong Yang Tarsha Ward Ke Ruan Jianchun Zhang Quan Wu Ping He Xia Ding Dongmei Wang Chuanhai Fu Zhen Dou Feng Yan Wenwen Wang Xing Liu Xuebiao Yao MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical biology CAS Center for Excellence in Molecular Cell ScienceHefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of ChinaHefei 230027China Department of Physiology Morehouse School of MedicineAtlantaGA 30310USA School of Basic Medical Sciences Beijing University of Chinese MedicineBeijing 100029China Department of biology Duke University DurhamNC 27708USA Institute of ProteoGenomics Beijing 100029China Harvard Medical School BostonMA 02115USA

Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase ***,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains *** the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric *** sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during *** real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability *** biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic *** inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the ***,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone *** findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle *** CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis.

关键词： organoids.cell division central spindle CENP-E syntelin PRC1

Nucleic acids analysis

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Science China Chemistry 2021年第2期64卷 171-203页

作者： Yongxi Zhao Xiaolei Zuo Qian Li Feng Chen Yan-Ru Chen Jinqi Deng Da Han Changlong Hao Fujian Huang Yanyi Huang Guoliang Ke Hua Kuang Fan Li Jiang Li Min Li Na Li Zhenyu Lin Dingbin Liu Juewen Liu Libing Liu Xiaoguo Liu Chunhua Lu Fang Luo Xiuhai Mao Jiashu Sun Bo Tang Fei Wang Jianbin Wang Lihua Wang Shu Wang Lingling Wu Zai-Sheng Wu Fan Xia Chuanlai Xu Yang Yang Bi-Feng Yuan Quan Yuan Chao Zhang Zhi Zhu Chaoyong Yang Xiao-Bing Zhang Huanghao Yang Weihong Tan Chunhai Fan Institute of Analytical Chemistry and Instrument for Life Science The Key Laboratory of Biomedical Information Engineering of Ministry of EducationSchool of Life Science and TechnologyXi’an Jiaotong UniversityXi’an 710049China Institute of Molecular Medicine Shanghai Key Laboratory for Nucleic Acid Chemistry and NanomedicineRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative MoleculesInstitute of Translational MedicineShanghai Jiao Tong UniversityShanghai 200240China Cancer Metastasis Alert and Prevention center Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and ChemotherapyState Key Laboratory of Photocatalysis on Energy and EnvironmentCollege of ChemistryFuzhou UniversityFuzhou 350108China CAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China State Key Lab of Food Science and Technology International Joint Research Laboratory for Biointerface and BiodetectionSchool of Food Science and TechnologyJiangnan UniversityWuxi 214122China Faculty of Materials Science and Chemistry Engineering Research Center of Nano-Geomaterials of Ministry of EducationChina University of GeosciencesWuhan 430074China College of Chemistry and Molecular Engineering Biomedical Pioneering Innovation Center(BIOPIC)Beijing Advanced Innovation Center for Genomics(ICG)Peking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China division of physical biology CAS Key Laboratory of Interfacial Physics and TechnologyShanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201800China bioimaging center Shanghai Synchrotron Radiation FacilityZhangjiang LaboratoryShanghai Advanced

Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic *** the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological *** this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.

关键词： nucleic acids analysis signal amplification DNA nanotechnology aptamer biosensing

The power of a healthy lifestyle for cancer prevention:the example of colorectal cancer

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Cancer biology & Medicine 2022年第11期19卷 1586-1597页

作者： Xuechen Chen Jie Ding Hengjing Li Prudence R.Carr Michael Hoffmeister Hermann Brenner division of Clinical Epidemiology and Aging Research German Cancer Research Center(DKFZ)Heidelberg 69120Germany Medical Faculty Heidelberg Heidelberg UniversityHeidelberg 69120Germany School of Public Health and Preventive MedicineMonash UniversityMelbourneVIC 3004Australia German Cancer Consortium(DKTK) German Cancer Research Center(DKFZ)Heidelberg 69120Germany division of Preventive Oncology German Cancer Research Center(DKFZ)and National Center forTumorDiseases(NCT)Heidelberg 69120Germany

Objective:We aimed to directly compare the estimated effects of adherence to a healthy lifestyle with those of risk predisposition according to known genetic variants affecting colorectal cancer(CRC)risk,to support effective risk communication for cancer ***:A healthy lifestyle score(HLS)was derived from 5 lifestyle factors:smoking,alcohol consumption,diet,physical activity,and body *** association of lifestyle and polygenic risk score(PRS)(based on 140 CRC-associated risk loci)with CRC risk was assessed with multiple logistic regression and compared through the genetic risk equivalent(GRE),a novel approach providing an estimate of the effects of adherence to a healthy lifestyle in terms of percentile differences in ***:A higher HLS was associated with lower CRC risk(4,844 cases,3,964 controls).Those adhering to all 5 healthy lifestyle factors had a 62%(95%CI 54%-68%)lower CRC risk than those adhering to≤2 healthy lifestyle *** estimated effect of adherence to all 5 compared with≤2 healthy lifestyle factors was as strong as the effect of having a 79 percentile(GRE 79,95%CI 61-97)lower *** association between a healthy lifestyle and CRC risk was independent of PRS level but was particularly pronounced among those with a family history of CRC in≥1 first-degree relative(P-interaction=0.0013).Conclusions:A healthy lifestyle was strongly inversely associated with CRC *** large GRE indicated that CRC risk determined by polygenic risk may be offset to a substantial extent by adherence to a healthy lifestyle.

关键词： Colorectal cancer healthy lifestyle score polygenic risk score family history genetic risk equivalent

Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization

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Protein & Cell 2021年第10期12卷 810-817页

作者： Yongcun Qu Qi Chen Shanshan Guo Chiyuan Ma Yonggang Lu Junchao Shi Shichao Liu Tong Zhou Taichi Noda Jingjing Qian Liwen Zhang Xili Zhu Xiaohua Lei Yujing Cao Wei Li Wei Li Nicolas Plachta Martin M.Matzuk Masahito Ikawa Enkui Duan Ying Zhang Hongmei Wang State Key Laboratory of Stem Cell and Reproductive biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China division of Biomedical Sciences School of MedicineUniversity of CaliforniaRiversideCA 92521USA Research Institute for Microbial Diseases Osaka UniversitySuitaOsakaJapan Department of Physiology and Cell biology Reno School of MedicineUniversity of NevadaRenoNVUSA Institute of Molecular and Cell biology A*STARSingaporeSin gapore center for Drug Discovery Department of Pathology and ImmunologyBaylor College of MedicineHoustonTX 77030USA division of biology and Biological Engineering California Institute of TechnologyPasadenaCAUSA Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China The Key Laboratory of Cell Proliferation and Regulation biology Ministry of EducationInstitute of Cell BiologyBeijing Normal UniversityBeijing 100875China

Dear Editor,Sperm cooperation has been observed in multiple species(Pizzari and Foster,2008),yet its existenee and benefit for reproductive success in mammals remains ***,combining tissue-clearing with deep three-dimensional(3D)imagi ng,we dem on strate that postcopulatory mouse sperm congregate into unidirectional sperm cooperative clusters at the utero-tubal junction(UTJ),a key physical barrier for passage into the oviduct.

关键词： passage media Foster

Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory drug naproxen

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Plant Communications 2023年第6期4卷 234-243页

作者： Jing Xia Mengjuan Kong Zhisen Yang Lianghanxiao Sun Yakun Peng Yanbo Mao Hong Wei Wei Ying Yongxiang Gao Jiri Friml Jianping Weng Xin Liu Linfeng Sun Shutang Tan MOE Key Laboratory for Cellular Dynamics Hefei National Laboratory for Physical Sciences at the MicroscaleThe First Affiliated Hospital of USTCBiomedical Sciences and Health Laboratory of Anhui ProvinceDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China Institute of Science and Technology Austria(ISTA) Am Campus 13400 KlosterneuburgAustria center for Advanced Interdisciplinary Science and Biomedicine of IHM Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China

The phytohormone auxin plays central roles in many growth and developmental processes in *** of chemical tools targeting the auxin pathway is useful for both plant biology and *** we reveal that naproxen,a synthetic compound with anti-inflammatory activity in humans,acts as an auxin transport inhibitor targeting PIN-FORMED(PIN)transporters in *** experiments indicate that exogenous naproxen treatment affects pleiotropic auxin-regulated developmental *** cellular and biochemical evidence indicates that naproxen suppresses auxin transport,specifically PIN-mediated auxin ***,biochemical and structural analyses confirm that naproxen binds directly to PIN1 protein via the same binding cavity as the indole-3-acetic acid ***,by combining cellular,biochemical,and structural approaches,this study clearly establishes that naproxen is a PIN inhibitor and elucidates the underlying *** use of this compound may advance our understanding of the molecular mechanisms of PIN-mediated auxin transport and expand our toolkit in auxin biology and agriculture.

关键词： auxin transport PIN naproxen Arabidopsis NPA

3D assessment of intervertebral disc degeneration in zebrafish identifies changes in bone density that prime disc disease

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Bone Research 2021年第4期9卷 521-536页

作者： Erika Kague Francesco Turci Elis Newman Yushi Yang Kate Robson Brown Mona S.Aglan Ghada A.Otaify Sarnia A.Temtamy Victor L.Ruiz-Perez Stephen Cross C.Patrick Royall P.Eckhard Witten Chrissy L.Hammond School of Physiology Pharmacology and NeuroscienceBiomedical SciencesUniversity of BristolBristolUK School of Physics HH Wills Physics LaboratoryUniversity of BristolBristolUK Centre for Nanoscience and Quantum Information University of BristolBristolUK Bristol Centre for Functional Nanomaterials University of BristolBristolUK Department of Anthropology and Archaeology University of BristolBristolUK Department of Mechanical Engineering University of BristolBristolUK Clinical Genetics Department Human Genetics and Genome Research DivisionCenter of Excellence for Human GeneticsNational Research CentreCairoEgypt lnstituto de Investigaciones Biomedicas de Madridand Ciber de Enfermedades Raras(CIBERER)MadridSpain Wolfson bioimaging Facility Biomedical SciencesUniversity of BristolBristolUK School of Chemistry University of BristolBristolUK Evolutionary Developmental biology Department of BiologyGhent UniversityGhentBelgium

Back pain is a common condition with a high social impact and represents a global health *** disc disease(IVDD)is one of the major causes of back pain;no therapeutics are currently available to reverse this *** impact of bone mineral density(BMD)on IVDD has been controversial,with some studies suggesting osteoporosis as causative for IVDD and others suggesting it as protective for *** studies to evaluate the influence of genetic components of BMD in IVDD could highlight opportunities for drug development and *** taking a holistic 3D approach,we established an aging zebrafish model for spontaneous *** BMD in aging,detected by automated computational analysis,is caused by bone deformities at the ***,aged zebrafish spines showed changes in bone morphology,microstructure,mineral heterogeneity,and increased fragility that resembled *** of the discs recapitulated IVDD symptoms found in humans:the intervertebral ligament(equivalent to the annulus fibrosus)showed disorganized collagen fibers and herniation,while the disc center(nucleus pulposus equivalent)showed dehydration and cellular *** manipulated BMD in young zebrafish by mutating sp7 and cathepsin K,leading to low and high BMD,***,we detected IVDD in both groups,demonstrating that low BMD does not protect against IVDD,and we found a strong correlation between high BMD and *** learning was applied to high-resolution synchrotron\iCJ image data to analyze osteocyte 3D lacunar distribution and morphology,revealing a role of sp7 in controlling the osteocyte lacunar 3D *** findings suggest potential avenues through which bone quality can be targeted to identify beneficial therapeutics for IVDD.

关键词： intervertebral degeneration protective

Developing biolmaging and quantitative methods to study 3D genome

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Frontiers of Electrical and Electronic Engineering in China 2016年第2期4卷 129-147页

作者： Juntao Gao Xusan Yang Mohamed Nadhir Djekide Yang Wang Peng Xi Michael Q. Zhang MOE Key Laboratory of Bioinformatics BioinformatJcs Division and Center for Synthetic & Systems Biology TNLIST Department of Automation Tsinghua University Beijing 100084 China Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China De~)artment of Biological Sciences Center for SystemsBiology.the University of Texas at Dallas Richardson TX 75080-3021USA

The recent advances in chromosome configuration capture （3C）-based series molecular methods and optical super- resolution （SR） techniques offer powerful tools to investigate three dimensional （3D） genomic structure in prokaryotic and eukaryotic cell nucleus. In this review, we focus on the progress during the last decade in this exciting field. Here we at first introduce briefly genome organization at chromosome, domain and sub-domain level, respectively; then we provide a short introduction to various super-resolution microscopy techniques which can be employed to detect gcnome 3D structure. We also reviewed the progress of quantitative and visualization tools to evaluate and visualize chromatin interactions in 3D genome derived from Hi-C data. We end up with the discussion that imaging methods and 3C-based molecular methods are not mutually exclusive -- actually they arc complemental to each other and can be combined together to study 3D genome organization.

关键词： 3D Genome quantitative methods bioimaging super resolution

A TOP6BL mutation abolishes meiotic DNA double-strand break formation and causes human infertility

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Science Bulletin 2020年第24期65卷 2120-2129,M0006页

作者： Yuying Jiao Suixing Fan Nazish Jabeen Huan Zhang Ranjha Khan Ghulam Murtaza Hanwei Jiang Asim Ali Yang Li Jianqiang Bao Beibei Zhang Jianze Xu Bo Xu Hafiz Muhammad Jafar Hussain Qumar Zaman Ihsan Khan Ihtisham Bukhari Furhan Iqbal Ayesha Yousaf Sobia Dil Manan Khan Niaz Ahmad Hui Ma Xiaohua Jiang Yuanwei Zhang Qinghua Shi First Affiliated Hospital of University of Science and Technology of China Hefei National Laboratory for Physical Sciences at Microscalethe CAS Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesDivision of Life Sciences and MedicineCAS Center for Excellence in Molecular Cell ScienceCollaborative Innovation Center of Genetics and DevelopmentUniversity of Science and Technology of ChinaHefei 230027China Institute of Pure and Applied biology Zoology DivisionBahauddin Zakariya UniversityMultan 60800Pakistan Shahbaz Sharif District Hospital Multan 60800Pakistan

Meiosis is pivotal for sexual reproduction and fertility. Meiotic programmed DNA double-strand breaks(DSBs) initiate homologous recombination, ensuring faithful chromosome segregation and generation of gametes. However, few studies have focused on meiotic DSB formation in human ***, we report four infertile siblings born to a consanguineous marriage, with three brothers suffering from non-obstructive azoospermia and one sister suffering from unexplained infertility with normal menstrual cycles and normal ovary sizes with follicular activity. An autosomal recessive mutation in TOP6BL was found co-segregating with infertility in this family. Investigation of one male patient revealed failure in programmed meiotic DSB formation and meiotic arrest prior to pachytene stage of prophase *** models carrying similar mutations to that in patients recapitulated the spermatogenic abnormalities of the patient. Pathogenicity of the mutation in the female patient was supported by observations in mice that meiotic programmed DSBs failed to form in mutant oocytes and oocyte maturation failure due to absence of meiotic recombination. Our study thus illustrates the phenotypical characteristics and the genotype-phenotype correlations of meiotic DSB formation failure in humans.

关键词： Programmed meiotic DNA double-strand breaks TOP6BL mutation Meiotic DSB formation failure Human infertility Oocyte maturation failure Meiotic arrest

Re-detectable positive SARS-CoV-2 RNA tests in patients who recovered from COVID-19 with intestinal infection

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Protein & Cell 2021年第3期12卷 230-235页

作者： Wanyin Tao Xiaofang Wang Guorong Zhang Meng Guo Huan Ma Dan Zhao Yong Sun Jun He Lianxin Liu Kaiguang Zhang Yucai Wang Jianping Weng Xiaoling Ma Tengchuan Jin Shu Zhu The First Affiliated Hospital of USTC Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiChina Hefei National Laboratory for physical Sciences at Microscale the CAS Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiChina School of Data Science University of Science and Technology of ChinaHefeiChina CAS Centre for Excellence in Cell and Molecular biology University of Science and Technology of ChinaHefeiChina Key Laboratory for Medical and Health of the 13th Five-Year Plan Anhui Provincial Center for Disease Control and PreventionHefeiChina

Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCoV-2),a novel coronavirus that causes Coronavirus Disease 2019(COVID-19)(Yang and Wang,2020),has spread to more than 200 countries and infected more than 9,000,000 people as of Jun *** of thousands of patients with COVID-19 have recovered and been discharged from ***,there are reports of recovered patients who subsequently tested positive for SARSCoV-2 after discharge(re-detectable positive,RP)(An et al.,2020;Lan et al.,2020),and this has led to increasing focus on the mechanism(s)underlying RP.

关键词： patients acute respiratory