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Granulocyte-macrophage colony-stimulating factor DNA prime-protein boost strategy to enhance efficacy of a recombinant pertussis DNA vac-cine

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Acta Pharmacologica Sinica 2006年第11期27卷 1487-1494页

作者： Qing-tian LI~(2,3,5)Yong-zhang ZHU~(2,3,5)Jia-you CHU~(2,6)Ke DONG~3 Ping HE~3 Chun-yan FENG~3 Bao-yu HU~3 Shu-min ZHANG~4 Xiao-kui GUO~(3,6)2 Institute of Medical Biology,Peking Union Medical College and Chinese Academy of Medical Sciences,Kunming 650118,China 3 Department of Medical Microbiology and parasitology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China 4 Chinese National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China Institute of Medical Biology Peking Union Medical College and Chinese Academy of Medical Sciences Kunming China Department of Medical Microbiology and parasitology Shanghai Jiao Tong University School of Medicine Shanghai China Chinese National Institute for the Control of Pharmaceutical and Biological Products Beijing China

Aim:To investigate a new strategy to enhance the efficacy of a recombinantpertussis DNA *** strategy is co-injection with cytokine plasmids asprime,and boosted with purified homologous ***:A recombinantpertussis DNA vaccine containing the pertussis toxin subunit 1(PTS 1),fragmentsof the filamentous hemagglutinin(FHA)gene and pertactin(PRN)gene encodingfilamentous hemagglutinin and pertactin were ***/c mice were im-munized with several DNA vaccines and antigen-specific antibodies anti-PTS1,anti-PRN,anti-FHA,cytokines interleukin(IL)-10,IL-4,IFN-γ,TNF-α,and spleno-cyte-proliferation assay were used to describe immune ***:Therecombinant DNA vaccine could elicit similar immune responses in mice as that ofseparate plasmids encoding the 3 fragments,*** immunized withDNA and boosted with the corresponding protein elicited more antibodies thanthose that received DNA as *** particular,when the mice were co-immu-nized with murine granulocyte-macrophage colony-stimulating factor plasmidsand boosted with proteins,all 4 cytokines and the 3 antigen-specific antibodieswere significantly increased compared to the pVAX1 ***-PTS1,anti-FHA,IL-4 and TNF-α elicited in the colony stimulating factor(CSF)prime-proteinboost group showed significant increase compared to all the other ***:This prime and boost strategy has proven to be very useful in im-proving the immunogenicity of DNA vaccines against pertussis.

关键词： granulocyte-macrophage colony-stimulating factor pertussis DNA vaccines

Unsatisfactory Knowledge, Attitude and Practice on Leptospirosis among Army Personnel’s in Northeastern Malaysia

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Health 2020年第3期12卷 281-292页

作者： Siti Sara Y. Aziah B. D. Azwany Y. N. Mohd Nazri S. Zahiruddin W. M. Nabilah A. Siti Asma’ H. Zaliha I. Public Health Division Selangor State Health Department Ministry of Health Selangor Malaysia Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Department of Medical Microbiology & parasitology School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Population Health & Preventive Medicine discipline Faculty of Medicine Universiti Teknologi MARA Selangor Malaysia

Leptospirosis is a re-emerging zoonotic disease caused by infection of the bacteria belongs to Genus leptospira. It occurs in tropical, subtropical and temperate zones. It is also known as occupational-related disease as certain occupations are associated with the occurrence of leptospirosis. Army is identified as one of the high-risk occupations in contracting leptospirosis. This study was done to determine knowledge, attitude and practice (KAP) among army personnel on leptospirosis. A cross sectional study was conducted among 616 army personnel in four based camps in Northeastern Malaysia, who fulfilled the inclusion and exclusion criteria. A validated KAP questionnaire was used, consisting of knowledge, attitude and practice questions. More than half (52.8%) of the respondents had poor knowledge score. For the attitude score, almost the same proportions had satisfactory attitude. The same finding was seen in practice score, whereby those with good practice (54.9%) and bad practice (45.9%). In conclusion, the finding showed that almost all army personnel had unsatisfactory KAP, thus, it is recommended for the army to have a good health education program on leptospirosis among army personnel to protect the army personnel from risk of getting leptospirosis.

关键词： Army Personnel Leptospirosis Knowledge Attitude Practice

The Impact of Leptospirosis Health Education Module (LHEM) on Changes of Knowledge, Attitude and Practice (KAP) among Army Personnel in Northeastern Malaysia

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Open Journal of Epidemiology 2020年第2期10卷 146-157页

作者： Y. Siti Sara B. D. Aziah Y. N. Azwany S. Mohd Nazri W. M. Zahiruddin A. Nabilah H. Siti Asma’ I. Zaliha Public Health Division Selangor State Health Department Ministry of Health Selangor Malaysia Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Department of Medical Microbiology & parasitology School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Population Health & Preventive Medicine discipline Faculty of Medicine Universiti Teknologi MARA Selangor Malaysia

Community-based interventions in prevention and control of leptospirosis, have been uniformly unsuccessful. Individual counseling and health education play an important role in the prevention and control of the disease. The aim of this study was to compare the effect of intervention on the knowledge, attitude and practice (KAP) score on leptospirosis among the army personnel after receiving a health education given. A validated questionnaire was used to assess knowledge, attitude and practice at pre- and post-intervention. A total of 188 army personnel from the two camps involved in this study with 94 respondents in control and intervention groups respectively. Leptospirosis Health Education Module (LHEM) was given to the intervention group. There was significant improvement in knowledge score before and after intervention done (mean difference = 24.25, 95% CI: 21.93, 26.56;p −14.13, 95% CI: −15.82, −12.45;p −0.72, 1.55;p > 0.05). For the intervention effect, the subjects in intervention group had significantly higher mean attitude score as compared to the subjects in control group (mean difference = −2.12, 95% CI: −2.99, −1.24;p −0.47, 1.98;p > 0.05). For the intervention effect regardless of time, the subjects in intervention group had significantly higher mean practice score as compared to the subjects in control group (mean difference = 4.16, 95% CI: 2.80, 5.52;p < 0.001). The leptospirosis health education module was found to cause improvement in KAP score in the intervention group.

关键词： Army Personnel LHEM Knowledge Attitude Practice

Expression and Comparative Analysis of Genes Encoding Outer Membrane Proteins LipL21,LipL32 and OmpL1 in Epidemic Leptospires

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Acta Biochimica et Biophysica Sinica 2005年第10期37卷 7+650-656页

作者： Xiang-Yan ZHANG~(1#) Yang YU~(2,3#) Ping HE~1 Yi-Xuan ZHANG~(2,3) Bao-Yu HU~1 Yang YANG~1 Yi-Xin NIE~4 Xiu-Gao JIANG~4 Guo-Ping ZHAO~(2,3) Xiao-Kui GUO~(1*) 1 Department of Medical Microbiology and parasitology,Shanghai Jiaotong University,Shanghai 200025,China 2 Research Center of Biotechnology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 3 Chinese National Human Genome Center at Shanghai,Zhangjiang High Tech Park,Shanghai 201203,China 4 Institute for Infectious Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China Department of Medical Microbiology and parasitology Shanghai Jiaotong UniversityShanghai 200025 China Research Center of Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Chinese National Human Genome Center at Shanghai Zhangjiang High Tech ParkShanghai 201203 China Institute for Infectious Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206 China

Leptospiral outer membrane proteins (OMPs) are highly conserved in different species,andplay an essential role in the development of new immunoprotection and serodiagnosis *** genesencoding LipL21,LipL32 and OmpL1 were cloned from the complete genome sequence of Leptospirainterrogans serovar lai strain Lai and expressed in *** comparison analysis revealed that the threegenes were highly conserved among distinct epidemic leptospires,including three major epidemic speciesLeptospira interrogans,Leptospira borgpetersenii and Leptospira weilii,in *** analysis wasfurther performed to scrutinize 15 epidemic Leptospira reference strains using the antisera of the *** immunoblot assay and reverse transcription-polymerase chain reaction demonstrated that thesethree OMPs were conservatively expressed in pathogenic *** strains and other pathogenic ***,the use of these recombinant OMPs as antigens in enzyme-linked immunosorbent assay (ELISA)for serodiagnosis of leptospirosis was *** recombinant LipL32 and OmpL1 proteins showed ahigh degree of ELISA reactivity with sera from patients infected with *** strain Lai and otherpathogenic *** results may contribute to the identification of candidates for broad-rangevaccines and immunodiagnostic antigens in further research.

关键词： outer membrane protein expression comparative analysis epidemic leptospire

Seroprevalence of Leptospirosis among Army Personnel in Northeastern Malaysia

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Advances in Infectious Diseases 2020年第1期10卷 37-45页

作者： Y. Siti Sara B. D. Aziah Y. N. Azwany S. Mohd Nazri W. M. Zahiruddin A. Nabilah H. Siti Asma’ I. Zaliha A. Fairuz Public Health Division Selangor State Health Department Ministry of Health Selangor Malaysia Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Department of Medical Microbiology & parasitology School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Population Health & Preventive Medicine discipline Faculty of Medicine Universiti Teknologi MARA Selangor Malaysia Institute for Medical Research (IMR) Kuala Lumpur Malaysia

Leptospirosis is a zoonotic disease caused by pathogenic Leptospira. The disease affects various high-risk groups including army personnel, who are at risk because of field activities that bring them into close contact with zoonotic reservoirs. This cross-sectional study determined the seroprevalence of leptospirosis among 616 asymptomatic army personnel in Northeastern Malaysia. Sociodemographic data were obtained using validated questionnaires. Serological screening for leptospirosis was performed using an ELISA method and confirmed by microscopic agglutination test (MAT). Samples indeterminate and positive according to ELISA (n = 117) were sent for MAT testing. A MAT titer ≥ 100 was considered positive evidence of exposure to leptospirosis. The overall seroprevalence of leptospirosis among the subjects was 16.2% (95% confidence interval: 13.32, 19.15). The most common reaction obtained with the sera tested by MAT was against a strain of Leptospira spp. isolated from Terengganu (38.3%) followed by L. biflexa serovar Patoc (35.2%). In conclusion, the seroprevalence of leptospirosis among army personnel in Northeastern Malaysia is high.

关键词： Seroprevalence Leptospirosis Army Personnel Northeastern Malaysia

Do Occupational Factors Render Army Service a High-Leptospirosis-Risk Occupation?

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Occupational Diseases and Environmental Medicine 2020年第2期8卷 79-89页

作者： Siti Sara Y. Aziah B. D. Azwany Y. N. Mohd Nazri S. Zahiruddin W. M. Nabilah A. Siti Asma’ H. Zaliha I. Fairuz A. Public Health Division Selangor State Health Department Ministry of Health Selangor Malaysia Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Department of Medical Microbiology & parasitology School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Population Health & Preventive Medicine discipline Faculty of Medicine Universiti Teknologi MARA Selangor Malaysia Institute for Medical Research (IMR) Kuala Lumpur Malaysia

Objective: Leptospirosis is a known zoonotic disease which is closely related to certain high-risk occupational group. This study aimed to identify the risk factors associated with seropositive leptospirosis among army personnel. Methodology: A cross sectional study was conducted involving 616 army personnel using a self-administered questionnaire. The questionnaire consisted of sociodemographic, occupational and environmental data as well as recreational activities history. Serum sample for leptospirosis was taken for screening with IgM Enzyme-linked Immunosorbent Assay (IgM ELISA) test. Positive tests were confirmed by Microscopic Agglutination Test (MAT). Results: All respondents were male, Malays and married with the mean age of 29.28 (7.15) years old. The mean duration of working was 11.50 (7.75). Majority of the respondents were non-officer and riflemen, smokers (455 (73.9%), stayed inside the camp and engaged in recreational activities. The mean duration of employment was 11.50 (7.75) years. The overall seroprevalence of leptospirosis was 16.2% (95% CI: 13.32, 19.15) and the predominant pathogenic serovar identified was Terengganu (38.2%). Those who had lower educational levels were more protective for positive leptospirosis compared to those who have only primary education (OR: 0.32;95% CI: 0.14, 0.76;p = 0.009). Conclusion: Despite being named as one of the high-risk occupations for leptospirosis, this study found no significant occupational factors related to leptospirosis seropositivity among army personnel.

关键词： Army Leptospirosis Seroprevalence Igm Enzyme-Linked Immunosorbent Assay (ELISA) Microscopic Agglutination Test (MAT)

Real-time PCR diagnosis of Schistosoma japonicum in low transmission areas of China

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Infectious Diseases of Poverty 2018年第1期7卷 87-97页

作者： Pei He Catherine A.Gordon Gail M.Williams Yuesheng Li Yuanyuan Wang Junjian Hu Darren J.Gray Allen G.Ross Donald Harn Donald P.McManus Hunan Institute of Parasitic Diseases YueyangPeople’s Republic of China Molecular parasitology Laboratory Infectious Diseases DivisionQIMR Berghofer Medical Research InstituteBrisbaneAustralia discipline of Epidemiology and Biostatistics School of Population HealthUniversity of QueenslandBrisbaneAustralia Research School of Population Health The Australian National UniversityCanberraAustralia Menzies Health Institute Queensland Griffith UniversityGold CoastQLDAustralia University of Georgia College of Veterinary MedicineAthensGAUSA.

Background:Schistosomiasis in the People’s Republic of China(PRC)can be traced back to *** the past 60 years,the Chinese government has made great efforts to control this persistent disease with elimination slated by 2020 through the implementation of a comprehensive control *** strategy aims to reduce the role of bovines and humans as sources of infection as a pre-requisite for elimination through transmission *** goal of elimination will be achievable only by the implementation of a sustainable surveillance and control system,with sensitive diagnosis a key feature so that the true disease burden is not *** used diagnostics lack the necessary sensitivity to accurately determine the prevalence of Schistosoma japonicum infection in areas with low infection *** is of critical importance to find and treat people and to identify animals with low-level infections if the National Control Programme for China is to achieve schistosomiasis ***:We evaluated a real-time polymerase chain reaction(qPCR)assay using 633 human stool samples collected from five villages in Hunan,Anhui,Hubei,and Jiangxi provinces,and 182 bovine(70 cattle and 112 buffalo)stool samples obtained from four villages in Hunan,Anhui,and Jiangxi provinces in the *** stool samples were subjected to the miracidium hatching test(MHT,a diagnostic procedure used in the National Schistosomiasis Control Programme)and the qPCR *** positive by MHT were subjected to either the Kato-Katz technique for humans,or the formalin-ethyl acetate sedimentation-digestion(FEA-SD)procedure for bovines,to determine infection ***:The qPCR assay exhibited a high level of sensitivity in the detection of *** *** both the human and bovine samples,a significantly higher prevalence was determined using the qPCR assay(11.06%humans,24.73%bovines)than with the MHT(0.93%humans,7.69%bovines).The animal contamination

关键词： Schistosomiasis Schistosoma Japonicum China Real-time PCR Miracidium hatching test Kato-Katz FEA-SD Bovine Human

Estimating the prevalence of Echinococcus in domestic dogs in highly endemic for echinococcosis

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Infectious Diseases of Poverty 2018年第1期7卷 761-769页

作者： Cong-Nuan Liu Yang-Yang Xu Angela M.Cadavid-Restrepo Zhong-Zi Lou Hong-Bin Yan Li Li Bao-Quan Fu Darren J.Gray Archie A.Clements Tamsin S.Barnes Gail M.Williams Wan-Zhong Jia Donald P.McManus Yu-Rong Yang State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary parasitology of Gansu Province/Key Laboratory of Zoonoses of Agriculture Ministry/Lanzhou Veterinary Research Institute CAASLanzhou 730046People’s Republic of China The Human Pathology and Immunology Department Ningxia Medical UniversityYinchuanNingxia Hui Autonomous RegionPeople’s Republic of China Neurosurgery Department Tianjin Xiqing HospitalTianjinPeople’s Republic of China Molecular parasitology Laboratory QIMR Berghofer Medical Research InstituteBrisbaneAustralia Research School of Population Health Australian National UniversityCanberraAustralia School of Population Health Infectious Disease Epidemiology UnitUniversity of QueenslandBrisbaneAustralia Queensland Alliance for Agriculture and Food Innovation University of QueenslandGattonAustralia

Background:Cystic echinococcosis(CE)and alveolar echinococcosis(AE)are highly endemic in Xiji County of Ningxia Hui Autonomous Region(NHAR)in China where the control campaign based on dog de-worming with praziquantel has been undertaken over preceding *** study is to determine the current prevalence of Echinococcus granulosus and *** in domestic dogs and monitor the echinococcosis transmission ***:Study villages were selected using landscape patterns(Geographic Information System,GIS)for Echinococcus transmission“hot spots”,combined with hospital records identifying risk areas for AE and CE.A survey of 750 domestic dogs,including copro-sampling and owner questionnaires,from 25 selected villages,was undertaken in 2012.A copro-multiplex PCR assay was used for the specific diagnosis of *** and *** in the *** analysis,using IBM SPSS Statistics,was undertaken,to compare the prevalence of the two Echinococcus *** dogs between four geographical areas of Xiji by theχ^(2)*** analysis of the combinations of outcomes from the questionnaire and copro-PCR assay data was carried out to determine the significant risk factors for dog ***:The highest de-worming rate of 84.0%was found in the northwest area of Xiji County,and significant differences(P0.05)in the prevalence of *** in dogs from the northwest,southwest,northeast,and southeast of Xiji,but there were significant differences(P<0.05)between dogs infected with *** from the four *** of the other independent variables was statistically ***:The results from this study indicate a high prevalenc

关键词： Domestic dog surveys Dog-copro-multiplex PCR assay Co-endemicity of Echinococcus granulosus and E.multilocularis Xiji County Ningxia hui autonomous region(NHAR) P.R.China

Maintenance of demographic and hematological profiles in a long-lasting dengue fever outbreak: implications for management

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Infectious Diseases of Poverty 2016年第1期5卷 776-786页

作者： Andréia Moreira dos Santos Carmo Rodrigo Buzinaro Suzuki Michele Marcondes Riquena André Eterovic árcia Aparecida Sperança Center for Natural and Human Sciences Universidade Federal do ABCCampus São Bernardo do Campo09606-070 São Bernardo Do CampoSão PauloBrasil Secretaria do Estado da Saúde do Estado de São Paulo Instituto Adolfo LutzCentro de Laboratório Regional VIIISanto André09040-160São PauloBrazil discipline of parasitology Marilia Medical SchoolMarilia 17519-030São PauloBrazil Center for Natural and Human Sciences Universidade Federal do ABCCampus Santo AndréSanto André09210-170São PauloBrazil

Background:Dengue fever(DF)outbreaks present regionally specific epidemiological and clinical *** certain medium-sized cities(100000–250000 inhabitants)of São Paulo State,Brazil,and after reaching an incidence of 150 cases/100000 inhabitants(“epidemiological threshold”),clinical diagnosis indicated dengue virus(DENV)*** this period,other seasonally infectious diseases with symptoms and physical signs mimicking DF can simultaneously occur,with the consequential overcrowding of health care facilities as the principal *** of clinical diagnosis of DF with serological tests may help in avoiding faulty diagnosis in patients,who might later undergo dengue hemorrhagic fever(DHF)and the dengue-shock syndrome(DSS).Furthermore,demographic and hematological profiles of patients are useful in detecting specific early characteristics associated to DF,DHF and ***:From March to June,2007,456 patients from Marilia in northwest São Paulo State who had only been diagnosed for DF by clinical criteria,underwent serologic testing for non-structural 1(NS1)DENV *** results were used in comparative analysis according to demographic(gender,age)and hematological(leukocyte and platelet counts,percentage of atypical lymphocytes)*** patterns were evaluated by subdividing data according to time of initial attendance,using recorded variables as predictors of DENV infection in logistic regression models and ROC ***:Serologic DENV detection was positive in 70.6%of the *** leukocyte and platelet counts were the most important factors in predicting DENV infection(respective medians DENV+=3715 cells/ml and DENV-=6760 cells/ml,and DENV+=134896 cells/ml and DENV-=223872 cells/ml).Furthermore,all demographic and hematological profiles presented a conservative temporal pattern throughout this long-lasting ***:As consistency throughout the epidemic facilitated defining the conservation

关键词： Clinical diagnosis Demographic profile Dengue fever Dengue-outbreak pattern Epidemiological threshold Hematological profile