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Susceptibility to Fluconazole and Ketoconazole of Candida spp. Isolated from Primary and Episodic Vulvovaginites by E-Test (São paulo, SP, Brazil)

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Open Journal of Obstetrics and Gynecology 2016年第12期6卷 689-697页

作者： Humberto Fabio Boatto Manoel João Batista Castello Girão Elaine Cristina Francisco Alexandre paulo Machado Maria Sayonara de Moraes Olga Fischman Department of Gynecology Faculty of Medicine Federal University of Sao Paulo-UNIFESP Sao Paulo Brazil Department of Microbiology Immunology and Parasitology Federal University of Sao Paulo-UNIFESP Sao Paulo Brazil Department of Basic Sciences in health Faculty of Medicine Federal University of Mato Grosso-UFMT Sao Paulo Brazil dermatologist of sao paulo state secretary of health Sao Paulo Brazil

Purpose: To evaluate the profile of in vitro susceptibility of yeasts isolated from cases of primary and espisodic vulvovaginitis to two antifungal agents. Methods: 40 Candida isolates from episodes of vulvovaginal candidiasis were identified by classic methodologies. The susceptibility testing of the in vitro fluconazole and ketoconazole activity against the isolates was accessed by E-test. Results: C. albicans was the most common species identified in 70% of the occurrences followed by C. glabrata (20%), C. tropicalis (7.5%), and C. guilliermondii (2.5%). In the susceptibility profile to antifungal agents, 12.5% and 16.7% of the isolates obtained from primary and episodic vulvovaginal candidiasis were resistant to fluconazole, respectively. To ketoconazole, we found that 6.25% and 12.5% of the isolates respectively from primary vulvovaginal candidiasis (PVVC) and episodic vulvovaginal candidiasis (EVVC) had high MIC values. Conclusions: E-test is a reliable method for the susceptibility testing of Candida spp. due to its simplicity, reproducibility, and lack of specialized equipment. Resistant strains and non-albicans species were verified more in cases of EVVC than in PVVC. Clinical and mycological cure of patients with episodic vulvovaginal candidiasis or complicated cases occurred after prolonged treatment and sometimes with multiple antifungals use.

关键词： Antifungal Susceptibility Candida Candidiasis Vulvovaginal E-Test Non-Albicans Species

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<i>In Vitro</i>Effects of Photodynamic Therapy with Three Photosensitizers on <i>Candida</i>spp from Complicated Vulvovaginitis and Asymptomatic Women

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Open Journal of Medical Microbiology 2021年第4期11卷 283-297页

作者： Humberto Fabio Boatto Maria Sayonara de Moraes Alexandre paulo Machado Manoel Joã o Batista Castelo Girã o Gilglécia Novaes Pereira Santana Fernanda Aparecida Vieira Fernandes Orion Sant’anna Motter Borba Olga Fischman Gompertz Faculty of Medicine University of Mogi das Cruzes Mogi das Cruzes S&#227 o paulo Brazil dermatologist of sao paulo state secretary of health Sao Paulo Brazil Department of Basic health Sciences Faculty of Medicine Federal University of Mato Grosso Cuiabá Brazil Departament of Ginecology Faculty of Medicine Federal University of S&#227 o paulo S&#227 o paulo Brazil Departament of Microbiology Imunology e Parasitology Federal University of S&#227 o paulo S&#227 o paulo Brazil

Aim: To evaluate photodynamic therapy (PDT) in vitro to reduce the growth of Candida spp, and its synergy with the antifungals fluconazole and ketoconazole for inhibition of resistant, DDS and susceptible isolates from asymptomatic carriers and with complicated vulvovaginitis. Methods: Between 2017 and 2020, we evaluated 230 women with vulvovaginal candidiasis and 400 asymptomatic. We obtained 130 positive cultures for Candida spp from vulvovaginitis and 94 asymptomatic. Yeasts were characterized by classical and molecular tests. Sensitivity to fluconazole and ketoconazole was evaluated by E-test. We used photodynamic light through blue LED, wavelengths between 450 to 470 nm, power of 260 mW, energy fluence of 270 J/cm2, for 15 minutes over all colonies of Candida spp. Methylene blue (MB) at 450 mg/mL, 2% gentian violet (VG) and 50

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Organic Coffee under Perspective of Energy Efficiency

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Journal of Agricultural Science and Technology(B) 2014年第1期4卷 15-20页

作者： Patricia Helena Nogueira Turco Osmar de Carvalho Bueno: Maura Seiko Tsutsui Esperancini Flavia Mariade Mello Bliska Department of Decentralization of Development Agriculture Secretary of S6o Paulo State Sao Paulo Agency of AgribusinessTechnology Campinas 13012-970 Sao Paulo Brazil Department of Economics and Rural Sociology Faculty of Agricultural Sciences of Botucatu Universidade Jfilio de MesquitaFilho Paulista State University Rua Josd Barbosa de Barros Botucatu 18603-970 Sao Paulo Brazil Agriculture secretary of sao paulo state Sao Paulo Agency of Agribusiness Technology Coffee Centre Agronomic InstituteCampinas 13012-970 Sao Paulo Brazil

The energy analysis development in this study contributes to the understanding of the dynamics of the organic coffee productive system, in particular to assess the independence of this system with respect to the use of industrialized input products. Thus, it provides information about the sustainability of that production system. Technical itineraries used in this study consist of energy expenditure made with coffee cultivation, according to the type, source and form of energy inputs, agricultural machines, equipment and labor force used in that production system. The energy expenditure, converted into energy units, quantified the input energy. And the organic coffee production, measured in kilograms of processed coffee beans, was the output energy. Primary data used in this study were obtained from organic coffee producers in the Southern region of Minas Gerais state, Brazil, in 2011. Energy balance identified was positive, since the estimated output energy was 626.465 MJ/ha and the energy expenditure was 112.998 MJ/ha, during the useful life of the crop.

关键词： Energetic balance sustainable production coffee cultivation.

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Oral health of children under chemotherapy treatment

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中国口腔颌面外科杂志 2008年第B5期6卷 206-207页

作者： Luiz E. R. Volpato Femanda V. Bezerra Femanda C. Ferreira Vivian B. Louzada Maria A. A. M. Machado Department of Dentistry for Special Care Patients Cuiabd Dental School University of Cuiaba. Cuiaba Mato Grosso Brazil Cuiaba Dental School University of Cuiaba. Cuiaba Mato Grosso Brazil Cuiaba Dental School University of Cuiaba. Cuiaba Mato Grosso Brazil Center of Dentistry for Special Care Patients Mato Grosso State Secretary of Health. Cuiaba Mato Grosso Brazil Department of Pediatric Dentistry Orthodontics and Collective Health Bauru Dental School Sao Paulo University. Bauru Sao Paulo Brazil

Purpose: To investigate the oral health status of children under chemotherapy and the effect of the treatment for childhood malignancies on oral health and on the presence and fi'equency of oral mucosa lesions。Methods: A total of 59±29 males and 30 females mean age 9.8±2.2 years old) children affected by cancer were evaluated。The children underwent clinical and radiographic examination, were alterations in teeth and soft tissues were recorded。Results: Caries prevalence was registered, and the mean DMFT was 3.0; mean value of Gingival Index was 1.16±0.55; xerostomia was present in 22.0% of the children and spontaneous oral bleeding in 14.0%。Oral mucositis was also present in 14.0% of the children。Conclusion: Oral health of children under chemotherapy treatment for different malignancies should be carefully followed due to the fi'equent manifestations of the therapy's side effects on the oral mucosa and to the difficulties to realize an oral treatment for those children due to their systemic condition。

关键词：口腔健康儿童化学疗法治疗方法

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Impacts of Coffee Production in Agroforestry System for Sustainable Development

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Journal of Agricultural Science and Technology(B) 2013年第8期3卷 535-544页

作者： Flavia Maria de Mello Bliska Patricia Helena Nogueira Turco Antonio Bliska Jfinior Daniel Carvalho Nepomuceno Agronomic Institute Coffee Centre S6o Paulo Agency of Agribusiness Technology Agriculture Secretary of Sao Paulo StateCampinas Sao Paulo 13012-970 Brazil Department of Decentralization of Development Silo Paulo Agency of Agribusiness Technology Agriculture Secretary of SaoPaulo State Campinas Sao Paulo 13012-970 Brazil Faculty of Agricultural Engineering University of Campinas Av. Cdndido Rondon 501-Bar6o Geraldo Campinas S6o Paulo13083-875 Brazil

This study estimates the environmental and socioeconomic impacts of coffee production in the agroforestry system in the settlements of farmers located in the West of the state of sao paulo, Brazil, in the region known as Pontal of Paranapanema, aiming at the development of programs to support the growth of production sustainable coffee and programs to protect the coffee activity before the possible global climate change. The settlements are located in areas with sandy soils and highly degraded by human action. Moreover, the region is considered marginal for the production of coffee, because it is subject to periods of high temperatures, prolonged drought and frost risk, which hinders the sustainability of farms. The environmental and socioeconomic impacts of agroforestry are analyzed through Ambitec-agro system, consisting of a set of indicators aimed at ex-post evaluation of the contribution of technological innovation to environmental, social and economic development of an agricultural activity. The system Ambitec involves: (1) general data collection about the technology; (2) application of questionnaires with adopters selected and insertion of data on impact indicators spreadsheets via platform MS-Excel and to obtain quantitative results and impacts partial indexes and aggregates the impact of technology selected; (3) analysis and interpretation of indices obtained, which vary between -15 and 15. The results indicate that agroforestry management can contribute to: (1) maintaining or increasing the sustainability of traditional coffee-growing regions in the case of sudden climate change; (2) the expansion of the coffee regions now considered unsuitable for the cultivation of coffee; (3) for sustainable regional development.

关键词：区域可持续发展农林复合系统咖啡生产社会经济影响全球气候变化农林业系统可持续性技术创新

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