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Increasing concentrations of dietary threonine, tryptophan, and glycine improve growth performance and intestinal health with decreasing stress responses in broiler chickens raised under multiple stress conditions

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ANIMAL NUTRITION 2024年第3期18卷 145-153页

作者： Kim, Hyun Woo Kim, Jong Hyuk Han, Gi Ppeum Kil, Dong Yong Chung Ang Univ Dept Anim Sci & Technol Anseong 17546 South Korea Chungbuk Natl Univ Dept Anim Sci Cheongju 28644 South Korea

The current study aimed to compare the effects of increasing concentrations of dietary threonine (Thr), tryptophan (Trp), and glycine (Gly) on growth performance, stress biomarkers, and intestinal function in broiler chickens under multiple stress conditions. Five hundred sixty broiler chickens at 21 d old were randomly allotted to 5 treatments with 8 replicates. Birds in a positive control (PC) treatment were raised under low stock density (16.9 birds/m(2) per cage) with recommended environmental conditions, whereas birds in 4 treatments were subjected to multiple stress conditions: a cyclic heat stress of 30 +/- 0.3 degrees C for 10 h and 23 +/- 0.2 degrees C for 14 h per day with high stock density (25.3 birds/m(2) per cage). A basal diet was assigned to both PC and negative control (NC) treatments. Three additional diets were individually formulated to contain double concentrations of digestible Thr, Trp, or Gly + Ser compared with their concentrations in the basal diet. The experiment lasted for 14 d. Results showed that NC treatment had less growth performance (P cell counts (P = 0.018), and trans-epithelial electrical resistance (TEER; P < 0.001), but greater (P = 0.026) feather corticosterone (CORT) concentrations than PC treatment. Thr treatment showed the least (P < 0.001) feed conversion ratio (FCR) among treatments under multiple stress conditions. Thr, Trp, and Gly treatments had less (P = 0.026) feather CORT concentrations, but had greater (P < 0.001) TEER than NC treatment. In conclusion, increasing concentrations of dietary Thr, Trp, or Gly improve the growth performance and intestinal health in broiler chickens with decreasing stress response under multiple stress conditions.

关键词： Broiler chicken Glycine Multiple stress Threonine Tryptophan MEAT QUALITY HEAT-STRESS BEHAVIORAL-RESPONSES OXIDATIVE STATUS SUPPLEMENTATION MICROFLORA INTEGRITY PROTEIN METABOLISM EXPRESSION

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Changes of uPA and uPA-R expression in the cornea after alkali burn

Changes of uPA and uPA-R expression in the cornea after alka...

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中国细胞生物学学会第八届会员代表大会暨学术大会

作者：严军杨恬李国平张艺曾益军杨进 Department of cell biology Third Military Medical University Chongqing 400038 P R China dept.of cell biology Third Military Medical Univ

In order to investigate the expression levels of uPA and uPA-R in corneal repair after alkali burn, we first established the corneal alkali burn models in vitro and in vivo, then we performed immunocytochemistry(ICC) of uPA /uPA-R and image analysis and statistical evaluation to determine their expression levels both in vitro and in vivo. Compared with control group, the expressions of uPA and uPA-R after alkali burn had no significant increases till 6h,then increased rapidly from 12h to 24h and reached their maxima at 24h. From 24h their expression levels decreased rapidly. In vivo they rebounded again after 48 hours and attained their second peaks at 96h respectively. After that, their expressions decreased again. TheuPA-positive reaction mainly distributed in the cytoplasm while that of uPA-R mostly distributed on the cellular membrane. Their expression changes were similar to each other, both in vivo and in vitro. In vivo, uPA and uPA-R expressed and gathereid in the basal layer of corneal epithelium. These results suggest that the time phase of 24h after wound is the typical stage associating with the expressional maximum of uPA and uPA-R both in vivo and in vitro. Furthermore, the results also imply that the expression changes of uPA and uPA-R correlate to the wound healing after the corneal alkali burn, uPA and uPA-R coordinate with each other to stimulate the wound healing.

关键词： uPA uPA-R cornea alkali burn

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Neural plasticity and adult neurogenesis: the deep biology perspective

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Neural Regeneration Research 2019年第2期14卷 201-205页

作者： Anna Maria Colangelo Giovanni Cirillo Lilia Alberghina Michele Papa Hans V.Westerhoff Laboratory of Neuroscience "R.Levi-Montalcini" Dept.of Biotechnology and Biosciences University of Milano-Bicocca SYSBIO Centre of Systems biology University of Milano-Bicocca NeuroMI Milan Center for Neuroscience University of Milano-Bicocca Laboratory of Morphology of Neuronal Network Department of Public Medicine University of Campania "Luigi Vanvitelli" Synthetic Systems biology and Nuclear Organization University of Amsterdam Molecular Cell Physiology VU University Amsterdam and Infrastructure Systems Biology at NL (ISBE.NL) Amsterdam NL and Systems Biology School for Chemical Engineering and Analytical Science University of Manchester

The recognition that neurogenesis does not stop with adolescence has spun off research towards the reduction of brain disorders by enhancing brain regeneration. Adult neurogenesis is one of the tougher problems of developmental biology as it requires the generation of complex intracellular and pericellular anatomies, amidst the danger of neuroinflammation. We here review how a multitude of regulatory pathways optimized for early neurogenesis has to be revamped into a new choreography of time dependencies. Distinct pathways need to be regulated, ranging from neural growth factor induced differentiation to mitochondrial bioenergetics, reactive oxygen metabolism, and apoptosis. Requiring much Gibbs energy consumption, brain depends on aerobic energy metabolism, hence on mitochondrial activity. Mitochondrial fission and fusion, movement and perhaps even mitoptosis, thereby come into play. All these network processes are interlinked and involve a plethora of molecules. We recommend a deep thinking approach to adult neurobiology.

关键词： neurogenesis adult brain neuroregeneration neuron differentiation nerve growth factor energy homeostasis mitochondria deep biology systems biology

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Performance of Sewage Sludge Treatment and Electricity Generation by Different Configuration Microbial Fuel cells

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Journal of Harbin Institute of Technology(New Series) 2013年第4期20卷 1-6页

作者： Jun-Qiu Jiang Kun Wang Xue-Xuan Peng Qing-Liang Zhao Yun-Shu Zhang Xiu-Dong Zhou dept.of Municipal and Environmental & Engineering Harbin Institute of Technology Institute of Fuzhou Electric Power Design

This paper compared the degradation efficiency of sludge organic matters and electric-production by two typical microbial fuel cells——dual-chamber microbial fuel cell(DMFC)and single chamber air cathode microbial fuel cell(SAMFC),and the variations of sludge protein,polysaccharide and ammonia nitrogen within the systems were also *** results showed that the concentration of sludge soluble chemical oxygen demand,protein and carbohydrate of DMFC are higher than these of SAMFC during the systems operation,while DMFC can achieve a better ammonia nitrogen removal than *** the same operation condition,the stable voltage output of DMFC and SAMFC is 0.61 V and 0.37 V;the maximum power density of DMFC and SAMFC is 2.79 W/m3and 1.25 W/m3;TCOD removal efficiency of DMFC and SAMFC is 34.14%and 28.63%for 12 d,***,DMFC has a higher coulomb efficiency than SAMFC,but both are less than5%.The results showed that DMFC present a better performance on sludge degradation and electric-production.

关键词： sewage sludge dual-chamber microbial fuel cell(DMFC) single chamber air cathode microbial fuel cell(SAMFC) degradation electricity production

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Precise Genome Engineering with Sequence-Specific Nucleases

Precise Genome Engineering with Sequence-Specific Nucleases

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第十二届全国植物基因组学大会

作者： Dan Voytas dept.of Genetics Cell Biology & Development and Center for Genome EngineeringUniversity of MinnesotaMinneapolisMN 55455USA

Sequence-specific nucleases are powerful tools for the targeted modification of plant *** a founding member of the Zinc Finger Consortium,our group has worked collaboratively to develop efficient methods for the design of zinc finger nucleases(ZFNs) suitable for plant genome *** our zinc finger reagents,we have demonstrated targeted gene modification by homologous recombination in tobacco at frequencies exceeding 1%of transformed *** have also created targeted gene knockouts in Arabidopsis and soybean through targeted DNA cleavage and imprecise repair of the cleaved chromosomes by non-homologous *** ZFN-induced mutations are germinally transmitted,enabling the recov-

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Maximizing aged photovoltaic array power: a computer modelling study

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CLEAN ENERGY 2024年第1期8卷 171-187页

作者： Sibai, Fadi N. Gulf Univ Sci & Technol Elect & Comp Engn Dept Mishref Kuwait

Photovoltaic (PV) modules age with time for various reasons such as corroded joints and terminals and glass coating defects, and their ageing degrades the PV array power. With the help of the PV array numerical model, this paper explores the effects of PV module ageing on the PV array power, and the power gains and costs of rearranging and recabling aged PV modules in a PV array. The numerical PV array model is first revised to account for module ageing, rearrangement and recabling, with the relevant equations presented herein. The updated numerical model is then used to obtain the array powers for seven different PV arrays. The power results are then analysed in view of the attributes of the seven PV array examples. A guiding method to recommend recabling after rearranging aged modules is then proposed, leading to further significant power gains, while eliminating intra-row mismatches. When certain conditions are met, it was shown that recabling PV modules after rearranging them may lead to further significant power gains, reaching 57% and 98% in two considered PV array examples. Higher gains are possible in other arrays. A cost-benefit analysis weighing annual power gains versus estimated recabling costs is also given for the seven considered PV array examples to guide recabling decisions based on technical and economic merits. In the considered examples, recabling costs can be recovered in <4 years. Compared with the powers of the aged arrays, power gains due to our proposed rearranging and recabling the PV arrays ranged between 73% and 131% in the considered examples-well over the gains reported in the literature. Moreover, the cost of our static module rearrangement and recabling method outshines the costs of dynamic reconfiguration methods recently published in the literature.

关键词： solar energy optimizing array power photovoltaic array modelling photovoltaic modules ageing photovoltaic module rearrangement module recabling and reconfiguration PV array mismatch POINT TRACKING MODULE RECONFIGURATION PERFORMANCE SIMULATION IMPACT cell

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Energy model based on solar potential and the production of electrical energy through living plants' photosynthesis

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CLEAN ENERGY 2024年第3期8卷 116-126页

作者： Martinez, Raymond Daniel Rodriguez Univ Metropolitana Fac Engn Dept Ind Prod Caracas 1073 Venezuela

Developing a sustainable energy model is imperative considering the current trend towards decarbonizing sectors worldwide. For this purpose, Venezuela was used as a reference to propose an energy model focused on taking advantage of plant photosynthesis through microbial-vegetable fuel cells together with an agro-photovoltaic system to enhance energy and agricultural production. Energy production from the cells was estimated using an average power density of 264 mW/m(2) over 4% of the areas destined for crops in the entire Venezuelan region, obtaining an annual production of 19.889 GWh/year. In contrast, the energy production of the agro-photovoltaic system was modelled using PVsyst software on 50% of the area used for the cells distributed throughout the states of Anzo & aacute;tegui, Gu & aacute;rico, Monagas and Portuguesa according to their meteorological conditions, solar irradiation and agricultural activity, resulting in 3 703 417 GWh/year. The resulting whole system proved to be able to cover >10 times the installed electricity generation capacity at a national level and, together with the tremendous scalability of the microbial fuel cells, it shows that Venezuela has a high potential for the production and distribution of clean energy. [GRAPHICS]

关键词： ecosystems microbial fuel cell microorganisms renewable sustainability RESOURCES

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Expression of Retroviral Mediated Wild Type p53 Gene in Laryngocarcinoma and Fibrosarcoma cell Lines and Its Inhibitory Effects on Cancer Growth

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High Technology Letters 1996年第1期2卷 103-106页

作者：陈晓巍 dept.of Otolaryngology Peking Union Medical College HospitalBeijing 100730P.R.China

The accumulated evidence indicates that head and neck cancers arise due to multiple genetic changes in both dominant oncogenes and tumor suppressor genes. In this report we examined whether the wild-type p53 gene was able to suppress in vitro and in vivo cellular growth of laryngocarcinoma cell line and fibrosarcoma which carried multiple genetic abnormalities. Introduction of retroviral mediated wild-type p53 complementary DNA into laryngocarcinoma cell line (Hep2) and fibrosarcoma cell line (MDAH 041) carrying either a homozygous deletion or a missense in the p53 gene greatly suppressed tumor cell growth and the malignant phenotype of the cancer cells was reversed.

关键词： P53 gene Laryngocarcinoma Fibrosacoma

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酵母中G-蛋白循环系统的数学建模和敏感性分析

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高等学校计算数学学报 2005年第S1期 61-66页

作者：邹秀芬 Tau-Mu Yi 武汉大学数学与统计学院 dept.of Developmental & cell biology University of California at IrvineCA 92697USA

1 引言 G-蛋白是高度保守在所有真核细胞内的重要信号转导分子,通常与受体分子结合负责把细胞外的信号精确无误地传递到细胞内,是细胞感知环境并产生或者切换相关反应的必不可少的分子开关．在动物细胞里,G-蛋白参与广泛的信号转导,如... 详细信息

1 引言 G-蛋白是高度保守在所有真核细胞内的重要信号转导分子,通常与受体分子结合负责把细胞外的信号精确无误地传递到细胞内,是细胞感知环境并产生或者切换相关反应的必不可少的分子开关．在动物细胞里,G-蛋白参与广泛的信号转导,如光、味觉、激素和生长因子等．G蛋白有两种构象:与GTP结合时的激活态和GDP结合时的失活态．通

关键词： two G-protein cycles yeast mathematical model sensitivity.

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Laser-Constructing 3D Copper Current Collector with Crystalline Orientation Selectivity for Stable Lithium Metal Batteries

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ENERGY & ENVIRONMENTAL MATERIALS 2024年第6期7卷 129-139页

作者： Li, Hui Wang, Gang Hu, Jin Li, Jun Huang, Jiaxu Xu, Shaolin Southern Univ Sci & Technol Dept Mech & Energy Engn Shenzhen 518000 Peoples R China

The practical application of lithium (Li) metal anodes in high-capacity batteries is impeded by the formation of hazardous Li dendrites. To address this challenge, this research presents a novel methodology that combines laser ablation and heat treatment to precisely induce controlled grain growth within laser-structured grooves on copper (Cu) current collectors. Specifically, this approach enhances the prevalence of Cu (100) facets within the grooves, effectively lowering the overpotential for Li nucleation and promoting preferential Li deposition. Unlike approaches that modify the entire surface of collectors, our work focuses on selectively enhancing lithiophilicity within the grooves to mitigate the formation of Li dendrites and exhibit exceptional performance metrics. The half-cell with these collectors maintains a remarkable Coulombic efficiency of 97.42% over 350 cycles at 1 mA cm(-2). The symmetric cell can cycle stably for 1600 h at 0.5 mA cm(-2). Furthermore, when integrated with LiFePO4 cathodes, the full-cell configuration demonstrates outstanding capacity retention of 92.39% after 400 cycles at a 1C discharge rate. This study introduces a novel technique for fabricating selective lithiophilic three-dimensional (3D) Cu current collectors, thereby enhancing the performance of Li metal batteries. The insights gained from this approach hold promise for enhancing the performance of all laser-processed 3D Cu current collectors by enabling precise lithiophilic modifications within complex structures.

关键词： copper current collector heat treatment laser processing lithium metal battery selective crystalline orientation ABNORMAL GRAIN-GROWTH LI METAL DEPOSITION TEXTURE ENERGY

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