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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy(PAR-SIM)

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Light(Science & Applications) 2024年第6期13卷 1140-1158页

作者： Xinzhu Xu Wenyi Wang Liang Qiao Yunzhe Fu Xichuan Ge Kun Zhao Karl Zhanghao Meiling Guan Xin Chen Meiqi Li Dayong Jin Peng Xi Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing100871China Wallace H.Coulter dept.of Biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332GAUSA National Biomedical Imaging Center Peking UniversityBeijing100871China Department of Biomedical Engineering College of EngineeringSouthern University of Science and TechnologyShenzhen518055GuangdongChina Airy Technologies Co. Ltd.Beijing100086China Eastern Institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315200China School of Life Science Peking UniversityBeijing100871China Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW2007Australia

Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological *** efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout *** this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate ***,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector *** using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm ***-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure ***,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 *** posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词： hardware illumination resolution

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Exosomes derived from tumor adjacent fibroblasts efficiently target pancreatic tumors

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Acta Pharmaceutica Sinica B 2024年第7期14卷 3009-3026页

作者： Saini Setua Shabia Shabir Poornima Shaji Ana Martinez Bulnes Anupam Dhasmana Swathi Holla Nivesh K.Mittal Nirakar Sahoo Tripti Saini Francesco Giorgianni Mohammad Sikander Andrew E.Massey Bilal B.Hafeez Manish K.Tripathi Vincent P.Diego Meena Jaggi Junming Yue Nadeem Zafar Murali M.Yallapu Stephen W.Behrman Sheema Khan Subhash C.Chauhan Department of Pharmaceutical Sciences College of Pharmacyand College of MedicineUniversity of Tennessee Health Science Center(UTHSC)MemphisTN 36163USA Department of Computer Science Islamic University of Science and TechnologyAwantiporaJ&K 192122India Department of Immunology and Microbiology School of MedicineUniversity of Texas Rio Grande ValleyMcAllenTX 78504USA South Texas Center of Excellence in Cancer Research School of Medicinethe University of Texas Rio Grande ValleyMcAllenTX 78504USA Himalayan School of Biosciences and Cancer Research Institute Himalayan Institute of Medical SciencesSwami Rama Himalayan UniversityDehradun 248016India Plough Center for Sterile Drug Delivery Solutions UTHSCMemphisTN 38104USA Department of biology College of SciencesUTRGVMcAllenTX 78539USA National Institute of Biomedical Imaging and Bioengineering(NIBIB) National Institutes of HealthBethesdaMD 20892USA South Texas Diabetes and Obesity Institute UTRGVMcAllenTX 78504USA dept.of Laboratory Medicine&Pathology University of WashingtonSeattleWA 98195USA Department of Surgery Baptist Memorial Medical EducationBaptist Memorial HospitalMemphisTN 38120USA Baptist Health Sciences University MemphisTN 38104USA

The application of extracellular vesicles,particularly exosomes(EXs),is rapidly expanding in the field of medicine,owing to their remarkable properties as natural carriers of biological *** study investigates utilization of exosomes derived from stromal cells of tumor adjacent normal tissues(NAF-EXs)for personalized medicine,which can be derived at the time of diagnosis by endoscopic ***,we show that exosomes(EXs)derived from NAFs demonstrate differential bio-physical characteristics,efficient cellular internalization,drug loading efficiency,pancreatic tumor targeting and delivery of ***-derived EXs(NAF-EXs)were used for loading ormeloxifene(ORM),a potent anti-cancer and desmoplasia inhibitor as a model *** found that ORM maintains normal fibroblast cell phenotype and renders them incompatible to be triggered for a CAF-like phenotype,which may be due to regulation of Ca^(2+) influx in fibroblast ***-EXs-ORM effectively blocked oncogenic signaling pathways involved in desmoplasia and epithelial mesenchymal transition(EMT)and repressed tumor growth in xenograft mouse *** conclusion,our data suggests preferential tropism of NAF-EXs for PDAC tumors,thus imply feasibility of developing a novel personalized medicine for PDAC patients using autologous NAF-EXs for improved therapeutic outcome of anti-cancer ***,it provides the opportunity of utilizing this biological scaffold for effective therapeutics in combination with standard therapeutic regimen.

关键词： Pancreatic cancer Tumor adjacent normal tissue fibroblasts(NAF) Desmoplasia Extracellular vesicles Exosomes Tumor targeting Ormeloxifene Personalized medicine

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How to package and SEND mRNA: a novel “humanized” vector system based on endogenous retroviruses

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Signal Transduction and Targeted Therapy 2021年第12期6卷 3367-3368页

作者： Kristoffer Riecken Dawid Głów Boris Fehse Research dept.cell&Gene Therapy Dept.of Stem Cell TransplantationUniversity Medical Centre Hamburg-Eppendorf(UKE)Hamburg 20246Germany

A recent publication in Science describes a novel mRNA delivery system termed SEND,entirely consisting of endogenous proteins.1 These virus-like particles(VLPs),able to deliver mRNA of e.g.,Cas9,represent a new class of gene transfer *** development of efficient,specific,and safe vectors for the delivery of nucleic acids and proteins remains one of the biggest challenges in gene therapy,particularly for in vivo *** currently preferred viral vectors have several limitations,including biosafety issues,laborious and expensive manufacture,as well as the impossibility of multiple applications due to the inherent immunogenicity of viral *** contrast,non-viral vectors are much easier to produce on a large scale,but have not yet reached gene transfer rates comparable with viral vectors.

关键词： endogenous system entirely

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Characteristics of the gene encoding pyrroline-5-carboxylate synthase(P5CS) in Vietnamese soybean cultivars(Glycine max ***)

Characteristics of the gene encoding pyrroline-5-carboxylate...

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International Conference on biology, Environment and Chemistry（ICBEC 2010）

作者： Chu Hoang Mau Nguyen Thi Thuy Huong Chu Hoang Lan Nguyen Tuan Anh Le Van Son Chu Hoang Ha dept.of Genetics Faculty of Biology Thainguyen University dept.of Genetics Faculty of Biology Vietnam National University of Science dept.of Environment Faculty of Agricultural Environment Thainguyen University Lab.of Plant cell Biotechnology Institute of Biotechnology

ISBN: (纸本)9789810886424

This research evaluates the ability of six soybean local cultivars and a control cultivar(DT84) to tolerate severe *** this research,SL5 and SL6 have the most stress tolerance while DT84 and SL1 have the least stress *** have isolated P5CS gene as it relates to stress tolerance by using PCR with *** results show that the size of the P5CS gene of the studied cultivars is 2,148 bp,which encodes for 715 amino *** these proteins are compared with the P5CS proteins of the other species,the result shows that the isolated P5CS proteins have a higher identity than other bean *** are located on the same branch of the phylogenetic ***(Pro) is one of the most accumulated osmolytes in salinity and water deficit conditions in plants.P5CS is a key regulatory enzyme involved in prolin biosynthesis in plants and is subject to feedback inhibition by *** conserved aspartate residue at 126 and the phenylalanine at 129 are involved in the feedback inhibition by Pro binding.A gene encoding a feedback insensitive P5CS enzyme was obtained by mutating the P5CS *** mutation was due to a change of an aspartate at position 125 to alanine in the *** mutated gene was cloned in pBT vector and *** gene will be transfered to plants to increase the level of proline overproduction and to enhance the drought tolerance ability.

关键词： drought,Glycine max,point mutation,P5CS,proline.

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Removal of nickel from spent electroless nickel-plating bath with nickel foam cathode

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Journal of Harbin Institute of Technology(New Series) 2002年第4期9卷 332-335页

作者：于秀娟郑彤魏杰周定 dept.of Environmental Engineering and Science

The electrochemical method was used to remove nickel ion from spent electroless nickel plating bath (pH=5 3). An electrolytic cell was composed of a porous nickel foam cathode and an inert RuO 2/Ti anode. Nickel ions were reduced and deposited on the surface of the nickel foam cathode. The effect of current density (i), linear velocity of wastewater(v), gap between cathode and anode(d C/A) and reaction time(t) on nickel removal rate and current efficiency were studied. As reaction time prolonged, nickel removal rate increased while current efficiency decreased. And larger v and smaller d C/A can enhance nickel removal rate and increase current efficiency by promoting mass transfer and dropping concentration polarization. The effect of current density on nickel removal by electrochemistry was related to other parameters. After three hours’ electrolysis with i=1 0 A/dm2, v=18 5 cm/min and d C/A=0 5 cm, nickel removal rate and current efficiency reached 85 6% and 29 1%, respectively.

关键词： spent electroless nickel plating bath electrochemical treatment nickel foam cathode

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Finding an Effective Distance Between T-cell and B-cell Using S/W ARQ in an Immune System Communication

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China Communications 2020年第1期17卷 174-185页

作者： Sanjit Ningthoujam Tekcham Chingkheinganba Swarnendu K Chakraborty dept.of Computer Science and Engineering NIT Arunachal Pradesh

In this paper,we present the effective distance between T-cell and B-cell in an immune system using Stop and Wait(S/W)Automatic Repeat Request(ARQ).The concentration of the molecules can be increased by increasing the transmitting number of molecules but it may reduce the performance of communication due to higher collision or interference with other *** is also reported in the literature that the concentration of the emitted molecules reduces if the distance from Transmitter(Tx)to Receiver(Rx)***,this paper mainly focuses on enhancing the receiver’s capture probability and higher successful complete transmission of the desired molecules by obtaining the effective distance from T-cell to *** order to find the effective distance,T-cell transmits the molecules 1(Interleukins-2)to B-cell,upon successful reception of molecules 1,antibodies(molecules 2)transmit back to ***,the effective distance of an immune system can be obtained after T-cell detects the concentration of the molecules 2 with respect to *** schemes of S/W ARQ protocols have implemented in Molecular Communication(MC)but it requires retransmission of duplicate copies due to the lack of addressing an effective ***,the simulations are performed in MATLAB and the results obtain higher capture probability and also successful complete transmission of the desired molecules.

关键词： T-cell B-cell MC antigens antibodies diffusion coefficient(D) round trip time(RTT)

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RTE, a novel regulator of the ethylene receptor ETRl in Arabidopsis

RTE, a novel regulator of the ethylene receptor ETRl in Arab...

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中国科学院植物分子生物学前沿国际研讨会

作者： Maximo Rivarola Josephine Resnick Jason Shockey dept.of cell biology and Molecular Genetics University of Maryland College Park dept.of cell biology and Molecular Genetics University of Maryland College Park dept.of cell biology and Molecular Genetics University of Maryland College Park

＜正＞Ethylene is a gaseous plant hormone that has profound effects on plant growth and development. Genetic analysis has been key to elucidating the ethylene signaling pathway. The isolation of ethylene response mutants in Arabidopsis has relied upon genetic screens for insensitive or constitutive response in dark-grown seedlings. In order to identify new components of the ethylene signaling pathway, a screen was carried out for suppressors of the dominant ethylene insensitive receptor mutant etr1-2.

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Impact of joint resource partitioning and routing on performance of relay enhanced cellular networks

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Journal of Harbin Institute of Technology(New Series) 2008年第4期15卷 465-469页

作者：刘涛戎蒙恬史宏逵 dept.of Electronic Engineering Shanghai Jiaotong University

Radio resource assignment schemes and routing strategies in relay enhanced cellular networks are proposed in this *** the reuse partitioning-based frequency planning framework,the intra-cell resource partitioning between the base station and relay nodes was addressed firstly by introducing a metric of effective reuse ***,coverage-oriented and capacity-oriented routings,as well as two link bandwidth assignment schemes" equal-bandwidth per link" and "equal-bandwidth per mobile station" were *** key issues and their impacts on the system performance were analyzed comprehensively and supported by *** show that the cell capacity and edge user throughput of the proposed network are superior to the traditional non-relay network when an appropriate effective reuse factor is adopted.

关键词：移动电话布线方法通信技术技术性能

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Development of the zebrafish circadian clock,and the embryonic events the clock controls

Development of the zebrafish circadian clock,and the embryon...

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2015年中国中西医结合学会时间生物医学专业委员会学术...

作者： David Whitmore Ricardo Laranjeiro Takako Katherine Tamai University College London Dept.of Cell and Developmental Biology

Zebrafish are an excellent model system in which to study the fundamental events of vertebrate *** contrast to mammals,large numbers of fertilized eggs develop outside of the *** embryos are also highly transparent,allowing imaging strategies to be readily *** such,zebrafish also represent a useful tool to study the development of the circadian clock.

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A New Traffic Model for Mobile Wireless Networks

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Journal of Shanghai Jiaotong university(Science) 2007年第4期12卷 471-476页

作者：赵方明蒋铃鸽何晨 dept.of Electronic Eng. Shanghai Jiaotong Univ.

A new traffic model for mobile wireless networks was given and analysed. In the model, it is considered of traffic characteristic for two special periods of time. A pre-emptive priority scheme combined with channel borrowing strategy according to the speciality was proposed. All the channels can be used by non-real-time services, and real-time services are given higher priority for they are allowed to pre-empt channels used by non-real-time services. Either real-time handoff requests or non-real-time handoff requests can be queued in queues when there is no channel can be used. Some channels are reserved for real-time handoff requests, which can also be used by non-real-time service when they are idle. Simulation results are also given. It is seen that our scheme performs better than other schemes when the arrival rate of real-time services is much higher than non-real-time services.

关键词： wireless network handoff channel allocation transition cell blocking probability

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