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Green Synthesis and Catalysis 2024年第1期5卷 35-41页

作者： Yajun Zhang Hao Wang Dandan Jiang Naixian Sun Wei He Lili Zhao Ning Qin Ning Zhu Zheng Fang Kai Guo College of biotechnology and Pharmaceutical Engineering State Key Laboratory of Materials-Oriented Chemical EngineeringNanjing Tech UniversityNanjing211800China Institute of Advanced Synthesis School of Chemistry and Molecular EngineeringNanjing Tech UniversityNanjing211800China Department of Mechanical Engineering The University of SheffieldSheffieldS13JDUnited Kingdom

The design and synthesis of a novel organic photocatalyst(OPC)have attracted broad interest from both the organic chemistry and polymer ***,we presented a visible-light-induced alkylarylation of alkenes strategy for core modification of 5,10-diaryl-5,10-dihydrophenazines(DADHPs)under metal-and additive-free conditions,with the aim to develop powerful photocatalyst for organocatalyzed atom transfer radical polymerization(O-ATRP).Good tolerance for a wide variety of functional groups and affording the corresponding tetrasubstituted products in moderate to good yields were achieved in the batch and flow *** experimental and computational mechanistic study confirmed that DADHPs’radical cations andπ-πstacking interactions played important roles in the alkylarylation of alkenes.A series of the tetrasubstituted DADHPs were directly synthesized from the corresponding noncore-modified phenazines,which were demonstrated to be effective organic photoredox catalysts for *** responsive nature,controlled polymerization manner,and welldefined poly(methyl methacrylate)were achieved via tetrasubstituted DADHPs mediated O-ATRP.

关键词： Photoinduced Core modification Diaryl dihydrophenzine O-ATRP

Evaluation of effects of 1,3-dinitrobenzene on sperm motility of hamster using computer assisted semen analysis (CASA)

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Asian Journal of Andrology 2001年第2期3卷 109-114页

作者： L.D.C.Peiris H.D.M.Moore dept. of Zoology University of Colombo Colombo 03 Sri LankaDept. of Molecular Biology and Biotechnology University of Sheffield Sheffield S 10 2TN UK

Aim: To evaluate the effects of 1,3-dinitrobenznene (mDNB) on sperm motility of hamster and to correlate the re-sults with the fertility. Methods: Adult male hamsters were gavaged with one of the 3 dose regimes of mDNB(1.5 mg daily for 4 weeks ,1.5 mg one day a week for 4 weeks and 1.0 mg 3 days a week for 4 weeks). Computerassisted semen analysis (CASA) was used to analyse the sperm motility parameters, curvilinear velocity (VCL) andstraight line velocity (VSL) of sperm in distal corpus epididymides and distal cauda epididymides. In vitro fertilisationwas carried out only for 1.5 mg mDNB daily group to determine the sperm fertilising capacity. Results: There wasa significant reduction in sperm velocity parameters at weeks 3 and 4 after treatment, which was correlated with a de-cline in sperm fertility. Conclusion; Sperm velocity parameters may be used to determine the effect of a toxic insulton the sperm function.

关键词： mDNB sperm motility CASA sperm fertility

Effects of acut and chronic doses of methoxy acetic acid on hamster sperm fertilising ability

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Asian Journal of Andrology 2001年第3期3卷 209-216页

作者： L.D.C.Peiris H.D.M.Moore dept. of Zoology University of Colombo Colombo 03 Sri LankaDept. of Molecular Biology and Biotechnology University of Sheffield Sheffield S10 2TN UK

Aim: To evaluate the effects of acute and chronic doses of methoxy acetic acid (MAA) on in vitro fertilisation byhamster sperm and to correlate the data with the testicular damage. Methods: Adult male hamsters were gavagedwith 3 single doses (0, 80, 160 and 650 mg/kg) and 3 chronic doses (0, 8, 32 and 64 mg/kg daily for 5 weeks) ofMAA in distilled water. After treatment hamsters were killed at weekly intervals and spermatozoa recovered from thedistal cauda epididymides were used to assess the fertilising capacity in vitro. The testes were processed for histologicalexamination. Results; Acute doses showed a significant reduction in sperm fertilising ability from week 3 and 4 af-ter treatment and with the chronic doses, the effects were more extensive and persistent. The results were in correpon-dence with the testicular damages observed. Conclusion: It is evident that both acute and chronic doses of MAAcan impair the sperm function by damaging one or more cell populations in the testis.(Asian J Androl 2001 Sep; 3: 209- 216)

关键词： methoxy acetic acid fertilisation in vitro testis spermatozoa fertility

Growth Enhancement of Dunaliella salina by Microbubble Induced Airlift Loop Bioreactor (ALB)—The Relation between Mass Transfer and Growth Rate

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Journal of Biomaterials and Nanobiotechnology 2013年第2期4卷 1-9页

作者： Kezhen Ying Daniel J. Gilmour Yuzhen Shi William B. Zimmerman Department of Chemical and Biological Engineering University of Sheffield Sheffield UK Department of molecular biology and biotechnology University of Sheffield Sheffield UK

The efficiency of a novel microalgal culture system (an airlift loop bioreactor [ALB] engaged with a fluidic oscillator to produce microbubbles) is compared with both a conventional ALB (producing fine bubbles without the fluidic oscillator) and non-aerated flask culture. The impact of CO2 mass transfer on Dunaliella salina growth is assessed, through varying the gas (5% CO2, 95% N2) dosing flow rate. The results showed that approximately 6 - 8 times higher chlorophyll content was achieved in the aerated ALB cultures than in the non-aerated flasks, and there was a 20% - 40% increase in specific growth rate of D. salina in the novel ALB with microbubbles when compared with the conventional ALB cultures. The increase in chlorophyll content was found to be proportional to the total amount of CO2 mass transfer. For the same dosing time and flow rate, higher CO2 mass transfer rate (microbubble dosing) resulted in a greater growth rate.

关键词： Airlift Bioreactor Fluidic Oscillator Dunaliella salina Specific Growth Rate

Enhanced Mass Transfer in Microbubble Driven Airlift Bioreactor for Microalgal Culture

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Engineering（科研） 2013年第9期5卷 735-743页

作者： Kezhen Ying Mahmood K. H. Al-Mashhadani James O. Hanotu Daniel J. Gilmour William B. Zimmerman Department of Chemical and Biological Engineering University of Sheffield Sheffield UK Department of molecular biology and biotechnology University of Sheffield Sheffield UK

In this study, the effect of microfluidic microbubbles on overall gas-liquid mass transfer (CO2 dissolution and O2 removal) was investigated under five different flow rates. The effect of different liquid substrate on CO2 mass transfer properties was also tested. The results showed that the KLa can be enhanced by either increasing the dosing flowrate or reducing the bubble size;however, increasing the flow rate to achieve a higher KLa would ultimately lower the CO2 capture efficiency. In order to achieve both higher CO2 mass transfer rate and capture efficiency, reducing bubble size (e.g. using microbubbles) has been proved more promising than increasing flow rate. Microbubble dosing with 5% CO2 gas showed improved KLa by 30% -?100% across different flow rates, compared to fine-bubble dosing. In the real algal culture medium, there appears to be two distinct stages in terms of KLa, divided by the pH of 8.4.

关键词： Microbubbles KLa CO2 Capture Algal Culture

Engineering Antibody Fragment with the Quantum Dot in Cancer Cell Imaging and Diagnosis

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Engineering（科研） 2012年第10期4卷 126-128页

作者： Lizhi Liu Weiming Xu Krebs Institute Department of Molecular Biology and Biotechnology University of Sheffield Western Bank Firth Court Sheffield UK

The conjugates of monoclonal antibodies and nanoparticles, including quantum-dot(QD), offer significant advantages over conventional fluorescent probes to image and study biological processes. The extend stability, intense fluorescence and low toxicity of QDs are well suited for biological applications. In the present study, we used QD-conjugated anti-glucose-regulated protein 78(GRP78) antibody to examine the gene expression in prostate cancer cells under the unfolded protein response (UPR). QDs have got unique, simple and fast properties over current diagnostic techniques such as peroxidase-based immunohistochemical staining procedures, therefore the nanocarrier-conjugated antibody fragment has potential to become a new therapeutic tool for cancer diagnosis and treatment.

关键词： Quantum Dot Antibody Fragment Prostate Cancer GRP78 scFv UPR Immunohistochemistry

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大自然为什么选择磷主导生命

中国科学：化学 2010年第7期40卷 927-939页

作者： BOWLER Matthew W. CLIFF Matthew J. WALTHO Jonathan P. BLACKBURN G.Michael Structural biology Group European Synchrotron Radiation FacilityF-38043 GrenobleFrance Krebs Institute Department of Molecular Biology and BiotechnologySheffield University

磷酸单酯和双酯被漫长的进化过程选择为生命体的核心.磷酸酯功能的多样性需要磷酰基转移酶不断地进化来实现.现今磷酰基转移酶的催化机制已经得到了广泛研究.通过高分辨X射线晶体衍射技术(X-ray)与19FNMR的联用,来研究磷酰基转移酶的金... 详细信息

磷酸单酯和双酯被漫长的进化过程选择为生命体的核心.磷酸酯功能的多样性需要磷酰基转移酶不断地进化来实现.现今磷酰基转移酶的催化机制已经得到了广泛研究.通过高分辨X射线晶体衍射技术(X-ray)与19FNMR的联用,来研究磷酰基转移酶的金属氟化物过渡态类似物,可以从原子层面上阐述磷酰基转移的机理.该催化机理充分地解释了磷酸酯的一对相互"矛盾"的性质,即磷酸酯结构的极其稳定性与磷酰基在信号传导过程中的灵活可调性.通过对元素周期表中其他元素的含氧酸根及其酯的性质的研究,目前还无法找出一个更加合适的元素来替代磷的这些重要功能,因而这些证据都支持了Todd的说法:"哪里有生命,哪里就一定有磷".

关键词：生物磷酸酯宇宙中的磷酸盐磷酰基转移机理激酶催化机理磷酸酶催化机理生命体选择磷酸盐磷酸酯的稳定性激酶和磷酸酶的调控

Biocatalysis of phosphate transfer:mechanistic knowledge aids inhibitor design

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Biocatalysis of phosphate transfer:mechanistic knowledge aid...

Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes

第六届全国化学生物学学术会议

作者： Nikky J.Baxter Matt Cliff G.Michael Blackburn Department of molecular biology & biotechnology Krebs InstituteSheffield UniversitySheffield S10 2TNEngland

The activities of 60%of all proteins are regulated by phosphorylation by kinases and dephosphorylation by *** control by inhibitors of kinases or phosphatases is a major target for biotechnology. However,phosphoryl transfer is the most difficult of all reactions catalysed by enzymes and is the subject of intensive mechanistic and structural *** effective acceleration of phosphate monoester hydrolysis by monoesterases is estimated to be close to 10(10 for tyrosyl phosphate).

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npj Flexible Electronics 2020年第1期4卷 109-116页

作者： Markos Athanasiadis Dzmitry Afanasenkau Wouter Derks Christoph Tondera Francesca Murganti Volker Busskamp Olaf Bergmann Ivan R.Minev biotechnology Center(BIOTEC) Center for Molecular and Cellular Bioengineering(CMCB)Technische Universität Dresden01307 DresdenGermany Center for Regenerative Therapies Dresden(CRTD) Center for Molecular and Cellular Bioengineering(CMCB)Technische Universität Dresden01307 DresdenGermany Department of Cell and molecular biology Karolinska InstitutetSE-17177 StockholmSweden Present address:Universitätsklinikum Bonn University of BonnBonnGermany Present address:Department of Automatic Control and Systems Engineering University of SheffieldMappin StreetSheffield S13JDUK These authors contributed equally:Markos Athanasiadis Dzmitry AfanasenkauWouter Derks

Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study,repair and engineering of cardiac *** are typically constructed from rigid and brittle materials processed in a cleanroom *** outstanding technological challenge is the integration of soft materials enabling a closer match to the mechanical properties of biological cells and *** we present an algorithm for direct writing of elastic membranes with embedded electrodes,optical waveguides and microfluidics using a commercial 3D printing system and a palette of silicone *** proof of principle,we demonstrate interfacing of cardiomyocytes derived from human induced pluripotent stem cells(hiPSCs),which are engineered to express *** demonstrate electrical recording of cardiomyocyte field potentials and their concomitant modulation by optical and pharmacological stimulation delivered via the *** work contributes a simple prototyping strategy with potential applications in organ-on-chip or implantable systems that are multi-modal and mechanically soft.

关键词： recording modal closer

Neural plasticity and adult neurogenesis: the deep biology perspective

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Neural Regeneration Research 2019年第2期14卷 201-205页

作者： Anna Maria Colangelo Giovanni Cirillo Lilia Alberghina Michele Papa Hans V.Westerhoff Laboratory of Neuroscience "R.Levi-Montalcini" Dept.of Biotechnology and Biosciences University of Milano-Bicocca SYSBIO Centre of Systems biology University of Milano-Bicocca NeuroMI Milan Center for Neuroscience University of Milano-Bicocca Laboratory of Morphology of Neuronal Network Department of Public Medicine University of Campania "Luigi Vanvitelli" Synthetic Systems biology and Nuclear Organization University of Amsterdam Molecular Cell Physiology VU University Amsterdam and Infrastructure Systems Biology at NL (ISBE.NL) Amsterdam NL and Systems Biology School for Chemical Engineering and Analytical Science University of Manchester

The recognition that neurogenesis does not stop with adolescence has spun off research towards the reduction of brain disorders by enhancing brain regeneration. Adult neurogenesis is one of the tougher problems of developmental biology as it requires the generation of complex intracellular and pericellular anatomies, amidst the danger of neuroinflammation. We here review how a multitude of regulatory pathways optimized for early neurogenesis has to be revamped into a new choreography of time dependencies. Distinct pathways need to be regulated, ranging from neural growth factor induced differentiation to mitochondrial bioenergetics, reactive oxygen metabolism, and apoptosis. Requiring much Gibbs energy consumption, brain depends on aerobic energy metabolism, hence on mitochondrial activity. Mitochondrial fission and fusion, movement and perhaps even mitoptosis, thereby come into play. All these network processes are interlinked and involve a plethora of molecules. We recommend a deep thinking approach to adult neurobiology.

关键词： neurogenesis adult brain neuroregeneration neuron differentiation nerve growth factor energy homeostasis mitochondria deep biology systems biology